Q8CSU3 (TOP1_STAES) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme

Gene names

Name:	topA
Ordered Locus Names:	SE_0926

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	DNA topoisomerase 1		PRO_0000285944
Regions
	Domain	3 – 112	110	Toprim
	Zinc finger	577 – 603	27	C4-type 1
	Zinc finger	617 – 645	29	C4-type 2
	Zinc finger	658 – 681	24	C4-type 3
Sites
	Active site	298	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CSU3 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 4F520E2D44D29A07	FASTA	689	79,519
	10 20 30 40 50 60 MADNLVIVES PAKAKTIEKY LGKRYKVIAS MGHVRDLPRS QMGVDTEDNY EPKYITIRGK 70 80 90 100 110 120 GPVVKDLKKH AKKAKKIFLA SDPDREGEAI AWHLSKILEL EDSKENRVVF NEITKDAVKD 130 140 150 160 170 180 SFKHPRGIEM DLVDAQQARR ILDRLVGYNI SPVLWKKVKK GLSAGRVQSV ALRLVIDREN 190 200 210 220 230 240 EIRNFKPEEY WSIEGEFRYK KSKFTAKFLH YKNKPYKLNN KDDVQRITEA LNGDQFEITN 250 260 270 280 290 300 VNRKEKTRYP AHPFTTSTLQ QEAARKLNFK ARKTMMLAQQ LYEGIDLKRQ GTVGLITYMR 310 320 330 340 350 360 TDSTRISTSA KSEAQQYIND KYGEQYVSQR KSSGKQGDQD AHEAIRPTST MRTPDDMKAF 370 380 390 400 410 420 LTRDQHRLYK LIWERFVASQ MAPAILDTVA LDVTQNDIKF RANGQTIKFK GFMTLYVEAK 430 440 450 460 470 480 DDKENDKENK LPQLDKGDKV TATKIEPAQH FTQPPPRYTE ARLVKTLEEL KIGRPSTYAP 490 500 510 520 530 540 TIDTIQKRNY VKLESKRFIP TELGEIVYEQ VKEYFPEIID VEFTVNMETL LDKIAEGDMN 550 560 570 580 590 600 WRKVIGDFYN SFKQDVERAE SEMEKIEIKD EPAGEDCEVC GSPMVIKMGR YGKFMACSNF 610 620 630 640 650 660 PDCRNTKAIV KTIGVTCPKC NEGDVVERKS KKNRIFYGCS RYPECDFISW DKPVGRDCPK 670 680 CHHYLVNKKK GKSSQVVCSN CDYEEEVQK

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO04523.1.
RefSeq	NP_764481.1. NC_004461.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ProteinModelPortal	Q8CSU3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CSU3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000039681; EBSTAP00000038314; EBSTAG00000039679.
GeneID	1057530.
GenomeReviews	Gene locus SE_0926 in contig AE015929_GR.
KEGG	sep:SE0926.
PATRIC	19607655. VBIStaEpi113981_0901.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00050000024420.
HOGENOM	HBG512935.
OMA	EEYWSID.
PhylomeDB	Q8CSU3.
ProtClustDB	PRK05582.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_0926-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 3 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_STAES

Accession

Primary (citable) accession number: Q8CSU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program