Reviewed, UniProtKB/Swiss-Prot Q8CSL7 (ARLS_STAES)

Names and origin

Protein names

Recommended name:
    Signal transduction histidine-protein kinase arlS
    EC=2.7.13.3

Gene names

Name:	arlS
Ordered Locus Names:	SE_1099

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of the two-component regulatory system arlS/arlR. ArlS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to arlR By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Signal transduction histidine-protein kinase arlS		PRO_0000293449
Regions
	Transmembrane	13 – 33	21	Potential
	Transmembrane	157 – 177	21	Potential
	Domain	179 – 232	54	HAMP
	Domain	240 – 456	217	Histidine kinase
Amino acid modifications
	Modified residue	243	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CSL7-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8ACF8A6CF9D7F77B	FASTA	456	52,860
	10 20 30 40 50 60 MIKRQKLKYK WMLITTLITF TTILLFCLII IFFLKDTLRS SEIDEAERSS NDIANLFHSK 70 80 90 100 110 120 SLSDISALDL NASLENFQEI LIYDDKGRKL IQTSNDNTLA YDNKIDFKHP ERIHIQRSHG 130 140 150 160 170 180 INYLVITEPI RSKDFSGYSV LVHSLQNYDN LVKSLYIVAL AFGLIATIIT AGVSYIFSSQ 190 200 210 220 230 240 ITKPIVTMSN KMNQIRRDGF QNKLELTTNY EETDNLIDTF NEMMYQIEES FNQQRQFVED 250 260 270 280 290 300 ASHELRTPLQ IIQGHLNLIQ RWGKKDPAVL EESLNISIEE VNRITKLVEE LLLLTKDRVN 310 320 330 340 350 360 HNVLECENVD VNSEIQSRVK SLQHLHPDYT FETHLATKPI QLKINRHQFE QLLLIFIDNA 370 380 390 400 410 420 MKYDTEHKHI KIVTQLKNKM IMIDITDHGM GIPKADLEFI FDRFYRVDKS RARSQGGNGL 430 440 450 GLSIAEKIVQ LNGGMIQVES ELQKYTTFKI SFPVLN

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015929 Genomic DNA. Translation: AAO04696.1.
RefSeq	NP_764654.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CSL7.
Genome annotation databases
GeneID	1057471.
GenomeReviews	Gene locus SE_1099 in contig AE015929_GR.
KEGG	sep:SE1099.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8CSL7.
OMA	FIDNAMK.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_1099-MON.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLS_STAES

Accession

Primary (citable) accession number: Q8CSL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)