Q8CS97 (RELA_STAES) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP pyrophosphokinase
EC=2.7.6.5

Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I

Gene names

Name:	relA
Ordered Locus Names:	SE_1315

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus epidermidis

Protein attributes

Sequence length	729 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp By similarity.
Catalytic activity	ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.
Sequence similarities	Belongs to the RelA/SpoT family. Contains 1 ACT domain. Contains 1 HD domain.

Ontologies

Keywords
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	guanosine tetraphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP diphosphokinase activity Inferred from electronic annotation. Source: EC kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 729	729	GTP pyrophosphokinase		PRO_0000166561
Regions
	Domain	50 – 149	100	HD
	Domain	654 – 727	74	ACT

Sequences

	Sequence		Length	Mass (Da)
	Q8CS97 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 2EEE5C9BEF92E0DA	FASTA	729	83,468
	10 20 30 40 50 60 MNNEYPYSAD EVLYKAKSYL STSEYEYVLK SYHIAYEAHK GQFRKNGLPY IMHPIQVAGI 70 80 90 100 110 120 LTEMRLDGPT IVAGFLHDVI EDTSYTFEDV KDMFNEEIAR IVDGVTKLKK VKYRSKEEQQ 130 140 150 160 170 180 AENHRKLFIA IAKDVRVILV KLADRLHNMR TLKAMPREKQ VRISKETLEI YAPLAHRLGI 190 200 210 220 230 240 NTIKWELEDT ALRYIDSVQY FRIVNLMKKK RSEREAYITN AINKIKNEMT KMNLSGEING 250 260 270 280 290 300 RPKHIYSIYR KMIKQKKQFD QIFDLLAIRI IVNSINDCYA TLGLVHTLWK PMPGRFKDYI 310 320 330 340 350 360 AMPKQNMYQS LHTTVVGPNG DPLEIQIRTH EMHEIAEHGV AAHWAYKEGK TVNQKTQDFQ 370 380 390 400 410 420 NKLNWLKELA ETDHTSADAQ EFMESLKYDL QSDKVYAFTP ASDVIELPYG AVPIDFAYAI 430 440 450 460 470 480 HSEVGNKMIG AKVNGKIVPI DYVLQTGDII EIRTSKHSYG PSRDWLKIVK SSSAKSKIKS 490 500 510 520 530 540 FFKKQDRSSN IEKGKFMVEA EIKEQGFRVE DILTEKNLEV VNEKYHFAND EDLYAAVGFG 550 560 570 580 590 600 GVTSIQIVNK LTERQRILDK QKALNEAQEV TKSVPIKKDI TTDSGVYVEG LENVLIKLSK 610 620 630 640 650 660 CCNPIPGDDI VGYITKGHGI KVHRTDCPNI KNETDRLISV EWVKSKDSTQ QYQVDLEVTA 670 680 690 700 710 720 YDRNGLLNEV LQAVNSTAGS LIKVSGRSDI DKNAVINISV MVKNVNDVYR VVEKIKQLGD VYTVSRVWN

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO04914.1.
RefSeq	NP_764870.1. NC_004461.1.
3D structure databases
ProteinModelPortal	Q8CS97.
SMR	Q8CS97. Positions 7-350.
ModBase	Search...
Protein-protein interaction databases
STRING	176280.SE1315.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO04914; AAO04914; SE_1315.
GeneID	1056237.
KEGG	sep:SE1315.
PATRIC	19608422. VBIStaEpi113981_1284.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0317.
KO	K00951.
OMA	LSWFREI.
ProtClustDB	CLSK885475.
Enzyme and pathway databases
UniPathway	UPA00908; UER00884.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR026020. (p)ppGpp_Synthase. IPR012675. Beta-grasp_dom. IPR003607. HD/PDEase_dom. IPR004811. RelA/Spo_fam. IPR007685. RelA_SpoT. IPR004095. TGS. IPR012676. TGS-like. [Graphical view]
PANTHER	PTHR21262. PTHR21262. 1 hit.
Pfam	PF04607. RelA_SpoT. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. SM00954. RelA_SpoT. 1 hit. [Graphical view]
SUPFAM	SSF81271. TGS-like. 1 hit.
TIGRFAMs	TIGR00691. spoT_relA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RELA_STAES

Accession

Primary (citable) accession number: Q8CS97

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program