ID   KPYK_STAES              Reviewed;         585 AA.
AC   Q8CS69;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   16-JUN-2009, entry version 37.
DE   RecName: Full=Pyruvate kinase;
DE            Short=PK;
DE            EC=2.7.1.40;
GN   Name=pyk; OrderedLocusNames=SE_1373;
OS   Staphylococcus epidermidis (strain ATCC 12228).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=176280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22832016; PubMed=12950922;
RX   DOI=10.1046/j.1365-2958.2003.03671.x;
RA   Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J.,
RA   Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z.,
RA   Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT   "Genome-based analysis of virulence genes in a non-biofilm-forming
RT   Staphylococcus epidermidis strain (ATCC 12228).";
RL   Mol. Microbiol. 49:1577-1593(2003).
CC   -!- CATALYTIC ACTIVITY: ATP + pyruvate = ADP + phosphoenolpyruvate.
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- COFACTOR: Potassium (By similarity).
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 5/5.
CC   -!- SIMILARITY: Belongs to the pyruvate kinase family.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the PEP-
CC       utilizing enzyme family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AE015929; AAO04972.1; -; Genomic_DNA.
DR   RefSeq; NP_764928.1; -.
DR   HSSP; P14178; 1E0T.
DR   GeneID; 1058009; -.
DR   GenomeReviews; AE015929_GR; SE_1373.
DR   KEGG; sep:SE1373; -.
DR   HOGENOM; Q8CS69; -.
DR   OMA; Q8CS69; ATESGYT.
DR   BioCyc; SEPI176280:SE_1373-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030955; F:potassium ion binding; IEA:InterPro.
DR   GO; GO:0004743; F:pyruvate kinase activity; IEA:EC.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:InterPro.
DR   InterPro; IPR008279; PEP_mobile.
DR   InterPro; IPR001697; Pyr_Knase.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase_cat.
DR   InterPro; IPR015794; Pyrv_Knase_a/b.
DR   InterPro; IPR018209; Pyrv_Knase_AS.
DR   InterPro; IPR015793; Pyrv_Knase_brl.
DR   Gene3D; G3DSA:3.50.30.10; PEP_mobile; 1.
DR   Gene3D; G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 1.
DR   Gene3D; G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1.
DR   PANTHER; PTHR11817; Pyruvate_kinase; 1.
DR   Pfam; PF00391; PEP-utilizers; 1.
DR   Pfam; PF00224; PK; 1.
DR   Pfam; PF02887; PK_C; 1.
DR   PRINTS; PR01050; PYRUVTKNASE.
DR   ProDom; PD001009; Pyruvate_kinase; 2.
DR   TIGRFAMs; TIGR01064; pyruv_kin; 1.
DR   PROSITE; PS00110; PYRUVATE_KINASE; FALSE_NEG.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glycolysis; Kinase; Magnesium;
KW   Metal-binding; Nucleotide-binding; Pyruvate; Transferase.
FT   CHAIN         1    585       Pyruvate kinase.
FT                                /FTId=PRO_0000294137.
FT   ACT_SITE    219    219       By similarity.
FT   METAL       221    221       Magnesium (By similarity).
FT   METAL       242    242       Magnesium (By similarity).
FT   METAL       243    243       Magnesium (By similarity).
SQ   SEQUENCE   585 AA;  63033 MW;  7756EDD80D49128C CRC64;
     MRKTKIVCTI GPASESEEML EKLMNAGMNV ARLNFSHGSH EEHKARIDTI RKVAKRLNKT
     IGLLLDTKGP EIRTHNMKDG LIVLEKGKEV IVSMNEVEGT PEKFSVTYEN LINDVNIGSY
     ILLDDGLVEL QVKEINKDKG EVKCDILNTG ELKNKKGVNL PGVKVNLPGI TDKDADDIRF
     GIKENVDFIA ASFVRRPSDV LDIRQILEEE KAEITIFPKI ENQEGIDNIE EILEVSDGLM
     VARGDMGVEI PPESVPMVQK DLIRKCNKLG KPVITATQML DSMQRNPRAT RAEASDVANA
     IYDGTDAVML SGETAAGQYP EEAVKTMRNI AVSAEAAQDY KKLLSDRTKL VETSLVNAIG
     VSVAHTALNL NVKAIVAATE SGSTARTISK YRPHSDIIAV TPSEKTARQC AIVWGVNPVV
     KEGRKTTDAL LNNAVATAVE TGRVSNGDLI IITAGVPTGE KGTTNMMKIH LVGDEIAKGQ
     GVGRGSVVGH AIVADSASDL EGKDLSDKVI ITNSVDETLV PYVEKAIGLI TEENGITSPS
     AIIGLEKGIP TVVGVEQATK EIKNDMLVTL DASQGKVFEG YANVL
//