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Protein

Pyruvate kinase

Gene

pyk

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2 (gapA2), Glyceraldehyde-3-phosphate dehydrogenase 1 (gapA1)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32SubstrateBy similarity1
Metal bindingi34PotassiumBy similarity1
Metal bindingi36PotassiumBy similarity1
Metal bindingi66PotassiumBy similarity1
Metal bindingi67Potassium; via carbonyl oxygenBy similarity1
Sitei219Transition state stabilizerBy similarity1
Metal bindingi221MagnesiumBy similarity1
Binding sitei244Substrate; via amide nitrogenBy similarity1
Metal bindingi245MagnesiumBy similarity1
Binding sitei245Substrate; via amide nitrogenBy similarity1
Binding sitei277SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciSEPI176280:G1G05-1379-MONOMER
UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Ordered Locus Names:SE_1373
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000001411 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002941371 – 585Pyruvate kinaseAdd BLAST585

Interactioni

Protein-protein interaction databases

STRINGi176280.SE1373

Structurei

3D structure databases

ProteinModelPortaliQ8CS69
SMRiQ8CS69
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated
In the C-terminal section; belongs to the PEP-utilizing enzyme family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9 Bacteria
COG0469 LUCA
COG3848 LUCA
KOiK00873
OMAiKHEAIEQ

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR008279 PEP-util_enz_mobile_dom
IPR036637 Phosphohistidine_dom_sf
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00391 PEP-utilizers, 1 hit
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52009 SSF52009, 1 hit
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit

Sequencei

Sequence statusi: Complete.

Q8CS69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKTKIVCTI GPASESEEML EKLMNAGMNV ARLNFSHGSH EEHKARIDTI
60 70 80 90 100
RKVAKRLNKT IGLLLDTKGP EIRTHNMKDG LIVLEKGKEV IVSMNEVEGT
110 120 130 140 150
PEKFSVTYEN LINDVNIGSY ILLDDGLVEL QVKEINKDKG EVKCDILNTG
160 170 180 190 200
ELKNKKGVNL PGVKVNLPGI TDKDADDIRF GIKENVDFIA ASFVRRPSDV
210 220 230 240 250
LDIRQILEEE KAEITIFPKI ENQEGIDNIE EILEVSDGLM VARGDMGVEI
260 270 280 290 300
PPESVPMVQK DLIRKCNKLG KPVITATQML DSMQRNPRAT RAEASDVANA
310 320 330 340 350
IYDGTDAVML SGETAAGQYP EEAVKTMRNI AVSAEAAQDY KKLLSDRTKL
360 370 380 390 400
VETSLVNAIG VSVAHTALNL NVKAIVAATE SGSTARTISK YRPHSDIIAV
410 420 430 440 450
TPSEKTARQC AIVWGVNPVV KEGRKTTDAL LNNAVATAVE TGRVSNGDLI
460 470 480 490 500
IITAGVPTGE KGTTNMMKIH LVGDEIAKGQ GVGRGSVVGH AIVADSASDL
510 520 530 540 550
EGKDLSDKVI ITNSVDETLV PYVEKAIGLI TEENGITSPS AIIGLEKGIP
560 570 580
TVVGVEQATK EIKNDMLVTL DASQGKVFEG YANVL
Length:585
Mass (Da):63,033
Last modified:March 1, 2003 - v1
Checksum:i7756EDD80D49128C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA Translation: AAO04972.1
RefSeqiNP_764928.1, NC_004461.1
WP_001830831.1, NC_004461.1

Genome annotation databases

EnsemblBacteriaiAAO04972; AAO04972; SE_1373
GeneIDi1058009
KEGGisep:SE1373
PATRICifig|176280.10.peg.1341

Similar proteinsi

Entry informationi

Entry nameiKPYK_STAES
AccessioniPrimary (citable) accession number: Q8CS69
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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