Reviewed, UniProtKB/Swiss-Prot Q8CS69 (KPYK_STAES)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40

Gene names

Name:	pyk
Ordered Locus Names:	SE_1373

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Sequence similarities	Belongs to the pyruvate kinase family. In the C-terminal section; belongs to the PEP-utilizing enzyme family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Pyruvate kinase		PRO_0000294137
Sites
	Active site	219	1	By similarity
	Metal binding	221	1	Magnesium By similarity
	Metal binding	242	1	Magnesium By similarity
	Metal binding	243	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CS69-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 7756EDD80D49128C	FASTA	585	63,033
	10 20 30 40 50 60 MRKTKIVCTI GPASESEEML EKLMNAGMNV ARLNFSHGSH EEHKARIDTI RKVAKRLNKT 70 80 90 100 110 120 IGLLLDTKGP EIRTHNMKDG LIVLEKGKEV IVSMNEVEGT PEKFSVTYEN LINDVNIGSY 130 140 150 160 170 180 ILLDDGLVEL QVKEINKDKG EVKCDILNTG ELKNKKGVNL PGVKVNLPGI TDKDADDIRF 190 200 210 220 230 240 GIKENVDFIA ASFVRRPSDV LDIRQILEEE KAEITIFPKI ENQEGIDNIE EILEVSDGLM 250 260 270 280 290 300 VARGDMGVEI PPESVPMVQK DLIRKCNKLG KPVITATQML DSMQRNPRAT RAEASDVANA 310 320 330 340 350 360 IYDGTDAVML SGETAAGQYP EEAVKTMRNI AVSAEAAQDY KKLLSDRTKL VETSLVNAIG 370 380 390 400 410 420 VSVAHTALNL NVKAIVAATE SGSTARTISK YRPHSDIIAV TPSEKTARQC AIVWGVNPVV 430 440 450 460 470 480 KEGRKTTDAL LNNAVATAVE TGRVSNGDLI IITAGVPTGE KGTTNMMKIH LVGDEIAKGQ 490 500 510 520 530 540 GVGRGSVVGH AIVADSASDL EGKDLSDKVI ITNSVDETLV PYVEKAIGLI TEENGITSPS 550 560 570 580 AIIGLEKGIP TVVGVEQATK EIKNDMLVTL DASQGKVFEG YANVL

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015929 Genomic DNA. Translation: AAO04972.1.
RefSeq	NP_764928.1.
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBase	Search...
Genome annotation databases
GeneID	1058009.
GenomeReviews	Gene locus SE_1373 in contig AE015929_GR.
KEGG	sep:SE1373.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8CS69.
OMA	Q8CS69. ATESGYT.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_1373-MON.
Family and domain databases
InterPro	IPR008279. PEP_mobile. IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.50.30.10. PEP_mobile. 1 hit. G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00391. PEP-utilizers. 1 hit. PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_STAES

Accession

Primary (citable) accession number: Q8CS69

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)