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Q8CS68

- PFKA_STAES

UniProt

Q8CS68 - PFKA_STAES

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (16 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111ATP; via amide nitrogenUniRule annotation
    Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
    Active sitei129 – 1291Proton acceptorUniRule annotation
    Binding sitei156 – 1561Allosteric activator ADPUniRule annotation
    Binding sitei164 – 1641Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei213 – 2131Allosteric activator ADPUniRule annotation
    Binding sitei224 – 2241SubstrateUniRule annotation
    Binding sitei245 – 2451Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 732ATPUniRule annotation
    Nucleotide bindingi102 – 1054ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSEPI176280:GCDG-1380-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:SE_1374
    OrganismiStaphylococcus epidermidis (strain ATCC 12228)
    Taxonomic identifieri176280 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000001411: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 322322ATP-dependent 6-phosphofructokinasePRO_0000111982Add
    BLAST

    Proteomic databases

    PRIDEiQ8CS68.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi176280.SE1374.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CS68.
    SMRiQ8CS68. Positions 1-322.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni127 – 1293Substrate bindingUniRule annotation
    Regioni171 – 1733Substrate bindingUniRule annotation
    Regioni187 – 1893Allosteric activator ADP bindingUniRule annotation
    Regioni215 – 2173Allosteric activator ADP bindingUniRule annotation
    Regioni251 – 2544Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    KOiK00850.
    OMAiMELREGH.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase_I_B1.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CS68-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKIAVLTSG GDSPGMNAAV RAVTRTAIYN NIEVYGVYQG YQGLLDDDIH    50
    KLELGSVGDT IQRGGTFLFS ARCPQFKEED VRKKAIENLR KRGIEGLVVI 100
    GGDGSYRGAQ RISEECKEIQ TIGIPGTIDN DINGTDFTIG FDTALNTIIE 150
    SVDKIRDTAS SHARTFIVEV MGRDCGDLAL WAGLSVGAET IVLPEVNTDI 200
    KDVAEKIEQG IKRGKKHSIV MVAEGCMSGQ ECADELTKYI NIDTRVSVLG 250
    HIQRGGSPSG ADRVLASRLG GYAVELLKQG ETAKGVGIRN NQLTSTPFDE 300
    IFAESDRKFN SQMYELAKEL SI 322
    Length:322
    Mass (Da):34,882
    Last modified:June 16, 2003 - v2
    Checksum:iBC40971A7BDD042E
    GO

    Sequence cautioni

    The sequence AAO04973.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015929 Genomic DNA. Translation: AAO04973.1. Different initiation.
    RefSeqiNP_764929.1. NC_004461.1.

    Genome annotation databases

    EnsemblBacteriaiAAO04973; AAO04973; SE_1374.
    GeneIDi1056395.
    KEGGisep:SE1374.
    PATRICi19608538. VBIStaEpi113981_1342.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015929 Genomic DNA. Translation: AAO04973.1 . Different initiation.
    RefSeqi NP_764929.1. NC_004461.1.

    3D structure databases

    ProteinModelPortali Q8CS68.
    SMRi Q8CS68. Positions 1-322.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 176280.SE1374.

    Proteomic databases

    PRIDEi Q8CS68.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO04973 ; AAO04973 ; SE_1374 .
    GeneIDi 1056395.
    KEGGi sep:SE1374.
    PATRICi 19608538. VBIStaEpi113981_1342.

    Phylogenomic databases

    eggNOGi COG0205.
    KOi K00850.
    OMAi MELREGH.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci SEPI176280:GCDG-1380-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase_I_B1.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
      Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
      Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 12228.

    Entry informationi

    Entry nameiPFKA_STAES
    AccessioniPrimary (citable) accession number: Q8CS68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: June 16, 2003
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3