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Q8CRU9

- MAP1_STAES

UniProt

Q8CRU9 - MAP1_STAES

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Protein
Methionine aminopeptidase
Gene
map, SE_1573
Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed By similarity.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei76 – 761Substrate By similarity
Metal bindingi93 – 931Divalent metal cation 1 By similarity
Metal bindingi104 – 1041Divalent metal cation 1 By similarity
Metal bindingi104 – 1041Divalent metal cation 2; catalytic By similarity
Metal bindingi168 – 1681Divalent metal cation 2; catalytic; via tele nitrogen By similarity
Binding sitei175 – 1751Substrate By similarity
Metal bindingi202 – 2021Divalent metal cation 2; catalytic By similarity
Metal bindingi233 – 2331Divalent metal cation 1 By similarity
Metal bindingi233 – 2331Divalent metal cation 2; catalytic By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciSEPI176280:GCDG-1618-MONOMER.

Protein family/group databases

MEROPSiM24.036.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase (EC:3.4.11.18)
Short name:
MAP
Short name:
MetAP
Alternative name(s):
Peptidase M
Gene namesi
Name:map
Ordered Locus Names:SE_1573
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000001411: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Methionine aminopeptidaseUniRule annotation
PRO_0000148961Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi176280.SE1573.

Structurei

3D structure databases

ProteinModelPortaliQ8CRU9.
SMRiQ8CRU9. Positions 1-249.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0024.
KOiK01265.
OrthoDBiEOG6MWNDS.

Family and domain databases

Gene3Di3.90.230.10. 1 hit.
HAMAPiMF_01974. MetAP_1.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CRU9-1 [UniParc]FASTAAdd to Basket

« Hide

MIVKTDEELQ ALKEIGYICA KVRDTMKEAT KPGVTTRELD HIAKDLFEEH    50
GAISAPIHDE NFPGQTCISV NEEVAHGIPG KRVIREGDLV NIDVSALKNG 100
YYADTGISFV VGKSDQPLKQ KVCDVATMAF ENAMKKVKPG TKLSNIGKAV 150
HATARQNDLT VIKNLTGHGV GQSLHEAPNH VMNYFDPKDK TLLKEGQVIA 200
VEPFISTHAT FVTEGKNEWA FETKDKSYVA QIEHTVIVTK DGPLLTTKID 250
D 251
Length:251
Mass (Da):27,550
Last modified:December 6, 2005 - v2
Checksum:i449764CBF1B98BB4
GO

Sequence cautioni

The sequence AAO05172.1 differs from that shown. Reason: Frameshift at position 5.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015929 Genomic DNA. Translation: AAO05172.1. Sequence problems.
RefSeqiNP_765128.1. NC_004461.1.

Genome annotation databases

EnsemblBacteriaiAAO05172; AAO05172; SE_1573.
GeneIDi1057259.
KEGGisep:SE1573.
PATRICi19609006. VBIStaEpi113981_1537.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015929 Genomic DNA. Translation: AAO05172.1 . Sequence problems.
RefSeqi NP_765128.1. NC_004461.1.

3D structure databases

ProteinModelPortali Q8CRU9.
SMRi Q8CRU9. Positions 1-249.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 176280.SE1573.

Protein family/group databases

MEROPSi M24.036.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO05172 ; AAO05172 ; SE_1573 .
GeneIDi 1057259.
KEGGi sep:SE1573.
PATRICi 19609006. VBIStaEpi113981_1537.

Phylogenomic databases

eggNOGi COG0024.
KOi K01265.
OrthoDBi EOG6MWNDS.

Enzyme and pathway databases

BioCyci SEPI176280:GCDG-1618-MONOMER.

Family and domain databases

Gene3Di 3.90.230.10. 1 hit.
HAMAPi MF_01974. MetAP_1.
InterProi IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view ]
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
PRINTSi PR00599. MAPEPTIDASE.
SUPFAMi SSF55920. SSF55920. 1 hit.
TIGRFAMsi TIGR00500. met_pdase_I. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
    Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
    Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 12228.

Entry informationi

Entry nameiMAP1_STAES
AccessioniPrimary (citable) accession number: Q8CRU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: May 14, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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