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Q8CRU5

- DAGK_STAES

UniProt

Q8CRU5 - DAGK_STAES

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Protein
Diacylglycerol kinase
Gene
dagK, SE_1583
Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthetic pathway By similarity.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Cofactori

Binds 1 magnesium ion per subunit. This ion appears to have a structural role and is required for catalytic activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATP By similarity
Binding sitei94 – 941ATP By similarity
Metal bindingi213 – 2131Magnesium; via carbonyl oxygen By similarity
Metal bindingi216 – 2161Magnesium By similarity
Metal bindingi218 – 2181Magnesium; via carbonyl oxygen By similarity
Active sitei273 – 2731Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 145ATP By similarity
Nucleotide bindingi67 – 737ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: InterPro
  3. diacylglycerol kinase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. phospholipid biosynthetic process Source: UniProtKB-KW
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSEPI176280:GCDG-1628-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAG kinase
Short name:
DAGK
Gene namesi
Name:dagK
Ordered Locus Names:SE_1583
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000001411: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Diacylglycerol kinase
PRO_0000386501Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi176280.SE1583.

Structurei

3D structure databases

ProteinModelPortaliQ8CRU5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 132132DAGKc
Add
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.

Phylogenomic databases

eggNOGiCOG1597.
KOiK07029.
OMAiYLKGMEM.
OrthoDBiEOG613098.

Family and domain databases

Gene3Di3.40.50.10330. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CRU5-1 [UniParc]FASTAAdd to Basket

« Hide

MRKRARIIYN PTSGKELFKR VLPDALIKLE KAGYETSAYA TEKIGDATFE    50
AERALESEYD LLIAAGGDGT LNEVVNGIAE QPNRPKLGVI PMGTVNDFGR 100
ALHLPSDIMG AIDVIIDGHT TKVDIGKMNN RYFINLAAGG KLTQVSYETP 150
SKLKSIVGPF AYYIKGFEML PQMKAVDVRI EYDDNIFQGE ALLFLLGLTN 200
SMAGFEKLVP DAKLDDGYFT LIIVEKANLA ELGHIMTLAS RGEHTKHPKV 250
IYAKAKSINI SSFTDMQLNV DGEYGGKLPA NFLNLEQHIE IFTPKDVFNE 300
ELLENDTITD ITPDKQ 316
Length:316
Mass (Da):34,950
Last modified:March 1, 2003 - v1
Checksum:iA3F39142BEB35A53
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015929 Genomic DNA. Translation: AAO05182.1.
RefSeqiNP_765138.1. NC_004461.1.

Genome annotation databases

EnsemblBacteriaiAAO05182; AAO05182; SE_1583.
GeneIDi1057085.
KEGGisep:SE1583.
PATRICi19609026. VBIStaEpi113981_1547.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015929 Genomic DNA. Translation: AAO05182.1 .
RefSeqi NP_765138.1. NC_004461.1.

3D structure databases

ProteinModelPortali Q8CRU5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 176280.SE1583.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO05182 ; AAO05182 ; SE_1583 .
GeneIDi 1057085.
KEGGi sep:SE1583.
PATRICi 19609026. VBIStaEpi113981_1547.

Phylogenomic databases

eggNOGi COG1597.
KOi K07029.
OMAi YLKGMEM.
OrthoDBi EOG613098.

Enzyme and pathway databases

BioCyci SEPI176280:GCDG-1628-MONOMER.

Family and domain databases

Gene3Di 3.40.50.10330. 1 hit.
InterProi IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
[Graphical view ]
Pfami PF00781. DAGK_cat. 1 hit.
[Graphical view ]
SMARTi SM00046. DAGKc. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
TIGRFAMsi TIGR00147. TIGR00147. 1 hit.
PROSITEi PS50146. DAGK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
    Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
    Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 12228.

Entry informationi

Entry nameiDAGK_STAES
AccessioniPrimary (citable) accession number: Q8CRU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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