Q8CRK4 (HDOX_STAES) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heme-degrading monooxygenase
EC=1.14.99.-

Alternative name(s):
Heme oxygenase
Iron-regulated surface determinant
Iron-responsive surface determinant

Gene names

Name:	isdG
Ordered Locus Names:	SE_1764

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus epidermidis

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the oxo-bilirubin chromophore staphylobilin (a mixture of the linear tetrapyrroles 5-oxo-delta-bilirubin and 15-oxo-beta-bilirubin) in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron By similarity. HAMAP-Rule MF_01272
Catalytic activity	Heme + 3 AH2 + 3 O2 = staphylobilins + Fe2+ + CO + 3 A + 3 H2O. HAMAP-Rule MF_01272
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase IsdG subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	heme catabolic process Inferred from electronic annotation. Source: HAMAP iron assimilation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heme binding Inferred from electronic annotation. Source: HAMAP heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 104	104	Heme-degrading monooxygenase HAMAP-Rule MF_01272		PRO_0000270097
Sites
	Metal binding	6	1	Iron By similarity
	Metal binding	76	1	Iron (heme axial ligand) By similarity
	Site	66	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CRK4 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: D4D94713D8EDF66F	FASTA	104	12,023
	10 20 30 40 50 60 MFVVTNRITV KKGYAKQMAP NFTKGGPIES LKGFEGIEVW QIDKDDYSED MYVNSWWETE 70 80 90 100 EDFKNWVNSD VFKQAHKNTG KSEDSPVIKS EIVKSNVLSS LNRR

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO05405.1.
RefSeq	NP_765319.1. NC_004461.1.
3D structure databases
ProteinModelPortal	Q8CRK4.
ModBase	Search...
Protein-protein interaction databases
STRING	176280.SE1764.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO05405; AAO05405; SE_1764.
GeneID	1057869.
KEGG	sep:SE1764.
PATRIC	19609404. VBIStaEpi113981_1722.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2329.
KO	K07145.
OMA	GFAEKMA.
ProtClustDB	PRK13315.
Family and domain databases
HAMAP	MF_01272. Heme_degrading_monooxygenase.
InterPro	IPR007138. Antibiotic_mOase. IPR011008. Dimeric_a/b-barrel. IPR023953. Heme-degrad_mOase. [Graphical view]
Pfam	PF03992. ABM. 1 hit. [Graphical view]
SUPFAM	SSF54909. Dimer_A_B_barrel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HDOX_STAES

Accession

Primary (citable) accession number: Q8CRK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program