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Protein

Oxygen sensor histidine kinase NreB

Gene

nreB

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Member of the two-component regulatory system NreB/NreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxygen, probably at the conserved histidine residue, and transfers its phosphate group probably to a conserved aspartate residue of NreC. NreB/NreC activates the expression of the nitrate (narGHJI) and nitrite (nir) reductase operons, as well as the putative nitrate transporter gene narT (By similarity).By similarity

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

[4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi58 – 581Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi61 – 611Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi73 – 731Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi76 – 761Iron-sulfur (4Fe-4S)Sequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSEPI176280:GCDG-2029-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen sensor histidine kinase NreB (EC:2.7.13.3)
Alternative name(s):
Nitrogen regulation protein B
Gene namesi
Name:nreB
Ordered Locus Names:SE_1970
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000001411 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Oxygen sensor histidine kinase NreBPRO_0000349339Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei158 – 1581Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi176280.SE1970.

Structurei

3D structure databases

ProteinModelPortaliQ8CR97.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini147 – 344198Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
KOiK07683.
OMAiRCEGYSN.
OrthoDBiEOG69GZGV.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR004358. Sig_transdc_His_kin-like_C.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
IPR017203. Sig_transdc_His_kinase_NreB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037432. STHK_NreB. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CR97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEQTDLSLE QLLKNYYETT NEKIVFVNRQ GKIIAMNDAA KDILTEEDNY
60 70 80 90 100
NAMTNAICHR CEGYSNEYDV QSCKDCFLET TQLQHSNFQV FMKTKDNEIK
110 120 130 140 150
PFTAMYQNID EQRGISAFTL QNVAPQIERQ EKMYQQKMLH RSIQAQENER
160 170 180 190 200
KRISRELHDS VIQDMLNIDV ELRLLKYKHR DKVLAETSQR IEGLLSQLID
210 220 230 240 250
DIRNMSVELR PSSLDDLGIE AAFKSYFKQF EENYGMHIKY DSNIKGMRFD
260 270 280 290 300
NEIETVVYRV VQEGVFNALK YAEVNEIEVS THSDGKQLVA EVVDRGKGFS
310 320 330 340
LDHHPKGSGL GLYGMRERAE LVNGHVNIET HINRGTIITL DIPI
Length:344
Mass (Da):39,871
Last modified:March 1, 2003 - v1
Checksum:i2F1D0199595716B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA. Translation: AAO05611.1.
RefSeqiNP_765525.1. NC_004461.1.
WP_002456059.1. NC_004461.1.

Genome annotation databases

EnsemblBacteriaiAAO05611; AAO05611; SE_1970.
GeneIDi1057872.
KEGGisep:SE1970.
PATRICi19609806. VBIStaEpi113981_1923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA. Translation: AAO05611.1.
RefSeqiNP_765525.1. NC_004461.1.
WP_002456059.1. NC_004461.1.

3D structure databases

ProteinModelPortaliQ8CR97.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176280.SE1970.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO05611; AAO05611; SE_1970.
GeneIDi1057872.
KEGGisep:SE1970.
PATRICi19609806. VBIStaEpi113981_1923.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
KOiK07683.
OMAiRCEGYSN.
OrthoDBiEOG69GZGV.

Enzyme and pathway databases

BioCyciSEPI176280:GCDG-2029-MONOMER.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR004358. Sig_transdc_His_kin-like_C.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
IPR017203. Sig_transdc_His_kinase_NreB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037432. STHK_NreB. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
    Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
    Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 12228.

Entry informationi

Entry nameiNREB_STAES
AccessioniPrimary (citable) accession number: Q8CR97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 1, 2003
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.