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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).By similarity

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.

Cofactori

Mg2+By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 218ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciSEPI176280:GCDG-987-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferase (EC:2.5.1.75)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferase
Short name:
DMAPP:tRNA dimethylallyltransferase
Short name:
DMATase
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name:
IPP transferase
Short name:
IPPT
Short name:
IPTase
Gene namesi
Name:miaA
Ordered Locus Names:SE_0981
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000001411 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332tRNA dimethylallyltransferasePRO_0000163978Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei105 – 1051Interaction with substrate tRNABy similarity

Protein-protein interaction databases

STRINGi176280.SE0981.

Structurei

Secondary structure

1
332
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 136Combined sources
Helixi20 – 3011Combined sources
Beta strandi33 – 375Combined sources
Turni49 – 524Combined sources
Turni56 – 616Combined sources
Beta strandi64 – 685Combined sources
Helixi78 – 9417Combined sources
Beta strandi98 – 1025Combined sources
Helixi106 – 1138Combined sources
Helixi125 – 13612Combined sources
Beta strandi139 – 1413Combined sources
Helixi143 – 15311Combined sources
Helixi155 – 1606Combined sources
Helixi166 – 17914Combined sources
Helixi184 – 1929Combined sources
Beta strandi196 – 2049Combined sources
Helixi207 – 22317Combined sources
Helixi226 – 23510Combined sources
Helixi243 – 2453Combined sources
Turni247 – 2515Combined sources
Helixi252 – 2576Combined sources
Helixi262 – 28625Combined sources
Beta strandi291 – 2944Combined sources
Turni295 – 2973Combined sources
Helixi300 – 31415Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3D3QX-ray2.70A/B1-332[»]
ProteinModelPortaliQ8CQL3.
SMRiQ8CQL3. Positions 6-315.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8CQL3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni16 – 216Substrate bindingBy similarity
Regioni39 – 424Interaction with substrate tRNABy similarity
Regioni167 – 1715Interaction with substrate tRNABy similarity
Regioni245 – 2506Interaction with substrate tRNABy similarity
Regioni278 – 2858Interaction with substrate tRNABy similarity

Sequence similaritiesi

Belongs to the IPP transferase family.Curated

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
KOiK00791.
OMAiHTRNYAK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CQL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEMTKPFLI VIVGPTASGK TELSIEVAKK FNGEIISGDS MQVYQGMDIG
60 70 80 90 100
TAKVTTEEME GIPHYMIDIL PPDASFSAYE FKKRAEKYIK DITRRGKVPI
110 120 130 140 150
IAGGTGLYIQ SLLYNYAFED ESISEDKMKQ VKLKLKELEH LNNNKLHEYL
160 170 180 190 200
ASFDKESAKD IHPNNRKRVL RAIEYYLKTK KLLSSRKKVQ QFTENYDTLL
210 220 230 240 250
IGIEMSRETL YLRINKRVDI MLGHGLFNEV QHLVEQGFEA SQSMQAIGYK
260 270 280 290 300
ELVPVIKGNI SMENAVEKLK QHSRQYAKRQ LTWFKNKMNV HWLNKERMSL
310 320 330
QMMLDEITTQ INKRSSNHDC KRKHPRPSTR EL
Length:332
Mass (Da):38,526
Last modified:March 1, 2003 - v1
Checksum:i8AFDFCA1DE3F9F55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA. Translation: AAO04578.1.
RefSeqiNP_764536.1. NC_004461.1.
WP_002439583.1. NC_004461.1.

Genome annotation databases

EnsemblBacteriaiAAO04578; AAO04578; SE_0981.
GeneIDi1057636.
KEGGisep:SE0981.
PATRICi19607764. VBIStaEpi113981_0955.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA. Translation: AAO04578.1.
RefSeqiNP_764536.1. NC_004461.1.
WP_002439583.1. NC_004461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3D3QX-ray2.70A/B1-332[»]
ProteinModelPortaliQ8CQL3.
SMRiQ8CQL3. Positions 6-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176280.SE0981.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO04578; AAO04578; SE_0981.
GeneIDi1057636.
KEGGisep:SE0981.
PATRICi19607764. VBIStaEpi113981_0955.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
KOiK00791.
OMAiHTRNYAK.

Enzyme and pathway databases

BioCyciSEPI176280:GCDG-987-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8CQL3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_STAES
AccessioniPrimary (citable) accession number: Q8CQL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.