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Protein

Beta sliding clamp

Gene

dnaN

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Beta sliding clamp
Short name:
Beta clamp
Short name:
Sliding clamp
Alternative name(s):
Beta-clamp processivity factor
DNA polymerase III beta sliding clamp subunit
DNA polymerase III subunit beta
Gene namesi
Name:dnaN
Ordered Locus Names:SE_0002
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000001411 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054691 – 377Beta sliding clampAdd BLAST377

Proteomic databases

PRIDEiQ8CQK6.

Interactioni

Subunit structurei

Forms a ring-shaped head-to-tail homodimer around DNA which binds and tethers DNA polymerases and other proteins to the DNA. The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase.By similarity

Protein-protein interaction databases

STRINGi176280.SE0002.

Structurei

3D structure databases

ProteinModelPortaliQ8CQK6.
SMRiQ8CQK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta sliding clamp family.Curated

Phylogenomic databases

eggNOGiENOG4105CZ8. Bacteria.
COG0592. LUCA.
KOiK02338.
OMAiNYEAVIP.

Family and domain databases

CDDicd00140. beta_clamp. 1 hit.
InterProiView protein in InterPro
IPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
PANTHERiPTHR30478. PTHR30478. 1 hit.
PfamiView protein in Pfam
PF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
PIRSFiPIRSF000804. DNA_pol_III_b. 1 hit.
SMARTiView protein in SMART
SM00480. POL3Bc. 1 hit.
TIGRFAMsiTIGR00663. dnan. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CQK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMEFTIKRDY FINQLNDTLK AISPRTTLPI LTGIKIDAKE NEVILTGSDS
60 70 80 90 100
EISIEITIPK QVDGEEIVEI TETGSVVLPG RFFVDIIKKL PGKEVKLSTN
110 120 130 140 150
EQFQTLITSG HSEFNLSGLD PDQYPLLPEV SRDDAIQLSV KVLKNIIAQT
160 170 180 190 200
NFAVSTSETR PVLTGVNWLI QDNELICTAT DSHRLAVRKL QLEDESENKN
210 220 230 240 250
VIIPGKALSE LNKIMSDSDE DIDIFFASNQ VLFRVGNINF ISRLLEGHYP
260 270 280 290 300
DTTRLFPENY EIKLGINNGD FYHAIDRASL LAREGGNNVI KLSTGNELVE
310 320 330 340 350
LSSTSPEIGT VKEEVNANDV EGGNLKISFN SKYMMDALKA IDNDEVEVEF
360 370
FGTMKPFILK PKDDDSVTQL ILPIRTY
Length:377
Mass (Da):42,102
Last modified:March 1, 2003 - v1
Checksum:i4188F2AFFD3BCBDD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA. Translation: AAO03599.1.
RefSeqiNP_763557.1. NC_004461.1.
WP_001831813.1. NC_004461.1.

Genome annotation databases

EnsemblBacteriaiAAO03599; AAO03599; SE_0002.
GeneIDi1056819.
KEGGisep:SE0002.
PATRICifig|176280.10.peg.2.

Similar proteinsi

Entry informationi

Entry nameiDPO3B_STAES
AccessioniPrimary (citable) accession number: Q8CQK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2003
Last modified: October 25, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families