Q8CQ77 (ISPD_STAES) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	SE_0319

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_0000075625
Sites
	Site	14	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	160	1	Positions MEP for the nucleophilic attack By similarity
	Site	217	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CQ77 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F9DBC597EE3C27EC	FASTA	238	26,799
	10 20 30 40 50 60 MIYAGILAGG IGSRMGNVPL PKQFLSLQGK PIIIHTVEKF LMYKDFDEII IATPQKWINY 70 80 90 100 110 120 MLDLLNNYQL DDKKIKVIQG GDDRNHSIMN IIESIEQHKK LNDEDIIVTH DAVRPFLTNR 130 140 150 160 170 180 IIRENVEYAS QYGAVDTVVN AVDTIISSND AQFISGIPIR SEMYQGQTPQ TFKIKELKDS 190 200 210 220 230 YLSLTQSQKE ILTDACKILV ELGKPVKLVK GELFNIKITT PYDLKVANSI ITGAVDND

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO03916.1.
RefSeq	NP_763874.1. NC_004461.1.
3D structure databases
ProteinModelPortal	Q8CQ77.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CQ77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000039070; EBSTAP00000037703; EBSTAG00000039068.
GeneID	1058046.
GenomeReviews	Gene locus SE_0319 in contig AE015929_GR.
KEGG	sep:SE0319.
PATRIC	19606431. VBIStaEpi113981_0294.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
GeneTree	EBGT00050000024510.
HOGENOM	HBG672839.
OMA	GELFNIK.
PhylomeDB	Q8CQ77.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_0319-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_STAES

Accession

Primary (citable) accession number: Q8CQ77

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program