Q8CQ25 (MDH_STAES) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	SE_0461

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus epidermidis

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000113472
Regions
	Nucleotide binding	13 – 18	6	NAD By similarity
	Nucleotide binding	124 – 126	3	NAD By similarity
Sites
	Active site	181	1	Proton acceptor By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	101	1	NAD By similarity
	Binding site	126	1	Substrate By similarity
	Binding site	157	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CQ25 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 85AC28A6477330B9	FASTA	315	34,476
	10 20 30 40 50 60 MLMVNRRKIS IIGAGHTGGT LAFILAQKEL GDIVLIERQQ SEGMAKGKAL DILESGPIWG 70 80 90 100 110 120 FDTSVHGSVN IEDIKDSDIV VMTAGIPRKS GMTREELVQT NEQIVRETAL QIATYAPHSI 130 140 150 160 170 180 IIVLTNPVDV MTYTAFKASG FPKERIIGQS GILDAARYRT FIAQELNVSV KDVNGFVLGG 190 200 210 220 230 240 HGDTMLPLIN NTHINGIPVK HLISEEKIDQ IVERTRKGGA EIVALLGQGS AYYAPATAIY 250 260 270 280 290 300 ETIDAIFNDR KRLLPSIAYL EGEYGCSDIC FGVPTIIGYQ GIEKIIEVDM NNDEYQQLQH 310 SAQAVSEVKN SLKFK

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO04058.1.
RefSeq	NP_764016.1. NC_004461.1.
3D structure databases
ProteinModelPortal	Q8CQ25.
SMR	Q8CQ25. Positions 6-311.
ModBase	Search...
Protein-protein interaction databases
STRING	176280.SE0461.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO04058; AAO04058; SE_0461.
GeneID	1056153.
KEGG	sep:SE0461.
PATRIC	19606711. VBIStaEpi113981_0434.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
KO	K00024.
OMA	DAMTYVM.
ProtClustDB	PRK06223.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_STAES

Accession

Primary (citable) accession number: Q8CQ25

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program