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Protein

Malate dehydrogenase

Gene

mdh

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881SubstrateUniRule annotation
Binding sitei94 – 941SubstrateUniRule annotation
Binding sitei101 – 1011NADUniRule annotation
Binding sitei126 – 1261SubstrateUniRule annotation
Binding sitei157 – 1571SubstrateUniRule annotation
Active sitei181 – 1811Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 186NADUniRule annotation
Nucleotide bindingi124 – 1263NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSEPI176280:GCDG-463-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:SE_0461
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Malate dehydrogenasePRO_0000113472Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi176280.SE0461.

Structurei

3D structure databases

ProteinModelPortaliQ8CQ25.
SMRiQ8CQ25. Positions 6-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
KOiK00024.
OMAiAGNVGEH.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CQ25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMVNRRKIS IIGAGHTGGT LAFILAQKEL GDIVLIERQQ SEGMAKGKAL
60 70 80 90 100
DILESGPIWG FDTSVHGSVN IEDIKDSDIV VMTAGIPRKS GMTREELVQT
110 120 130 140 150
NEQIVRETAL QIATYAPHSI IIVLTNPVDV MTYTAFKASG FPKERIIGQS
160 170 180 190 200
GILDAARYRT FIAQELNVSV KDVNGFVLGG HGDTMLPLIN NTHINGIPVK
210 220 230 240 250
HLISEEKIDQ IVERTRKGGA EIVALLGQGS AYYAPATAIY ETIDAIFNDR
260 270 280 290 300
KRLLPSIAYL EGEYGCSDIC FGVPTIIGYQ GIEKIIEVDM NNDEYQQLQH
310
SAQAVSEVKN SLKFK
Length:315
Mass (Da):34,476
Last modified:March 1, 2003 - v1
Checksum:i85AC28A6477330B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA. Translation: AAO04058.1.
RefSeqiNP_764016.1. NC_004461.1.
WP_011082618.1. NC_004461.1.

Genome annotation databases

EnsemblBacteriaiAAO04058; AAO04058; SE_0461.
GeneIDi1056153.
KEGGisep:SE0461.
PATRICi19606711. VBIStaEpi113981_0434.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA. Translation: AAO04058.1.
RefSeqiNP_764016.1. NC_004461.1.
WP_011082618.1. NC_004461.1.

3D structure databases

ProteinModelPortaliQ8CQ25.
SMRiQ8CQ25. Positions 6-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176280.SE0461.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO04058; AAO04058; SE_0461.
GeneIDi1056153.
KEGGisep:SE0461.
PATRICi19606711. VBIStaEpi113981_0434.

Phylogenomic databases

eggNOGiCOG0039.
KOiK00024.
OMAiAGNVGEH.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

BioCyciSEPI176280:GCDG-463-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
    Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
    Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 12228.

Entry informationi

Entry nameiMDH_STAES
AccessioniPrimary (citable) accession number: Q8CQ25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: March 1, 2003
Last modified: June 24, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.