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Q8CPQ1

- ATL_STAES

UniProt

Q8CPQ1 - ATL_STAES

Protein

Bifunctional autolysin

Gene

atl

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -[(Man)5(GlcNAc)2]-Asn structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact. Cleaves the peptidoglycan connecting the daughter cells at the end of the cell division cycle, resulting in the separation of the two newly divided cells. Acts as an autolysin in penicillin-induced lysis By similarity.By similarity

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
    Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

    GO - Molecular functioni

    1. amidase activity Source: InterPro
    2. mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Source: UniProtKB-EC
    3. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell wall macromolecule metabolic process Source: InterPro
    2. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciSEPI176280:GCDG-755-MONOMER.

    Protein family/group databases

    CAZyiGH73. Glycoside Hydrolase Family 73.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional autolysin
    Including the following 2 domains:
    N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
    Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (EC:3.2.1.96)
    Gene namesi
    Name:atl
    Ordered Locus Names:SE_0750
    OrganismiStaphylococcus epidermidis (strain ATCC 12228)
    Taxonomic identifieri176280 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000001411: Chromosome

    Subcellular locationi

    Secreted By similarity
    Note: Secreted, and then anchored on the cell surface at the peripheral cell wall above the completed septum (septal region), for the next cell division cycle.By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 13351306Bifunctional autolysinPRO_0000045481Add
    BLAST

    Post-translational modificationi

    Undergoes proteolytic processing to generate the two extracellular lytic enzymes, probably at the septal region on the cell surface.By similarity

    Interactioni

    Subunit structurei

    Oligomer; forms a ring structure at the cell surface which is important for efficient partitioning of daughter cells after cell division.By similarity

    Protein-protein interaction databases

    STRINGi176280.SE0750.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CPQ1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati516 – 6811661Add
    BLAST
    Repeati682 – 8451642Add
    BLAST
    Repeati846 – 10151703Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni303 – 863561N-acetylmuramoyl-L-alanine amidaseAdd
    BLAST
    Regioni864 – 1335472Endo-beta-N-acetylglucosaminidaseAdd
    BLAST

    Domaini

    The repeat domains R1, R2 and R3 are responsible for directing the proteins to the septal region.By similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.Curated
    In the C-terminal section; belongs to the glycosyl hydrolase 73 family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG4193.
    KOiK13714.
    OMAiKSGWISK.
    OrthoDBiEOG6GXTN7.

    Family and domain databases

    Gene3Di3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    IPR013338. Lysozyme_subfam2_dom.
    IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
    [Graphical view]
    PfamiPF01510. Amidase_2. 1 hit.
    PF01832. Glucosaminidase. 1 hit.
    [Graphical view]
    SMARTiSM00644. Ami_2. 1 hit.
    SM00047. LYZ2. 1 hit.
    [Graphical view]
    SUPFAMiSSF55846. SSF55846. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8CPQ1-1 [UniParc]FASTAAdd to Basket

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    MAKKFNYKLP SMVALTLFGT AFTAHQANAA EQPQNQSNHK NVLDDQTALK     50
    QAEKAKSEVT QSTTNVSGTQ TYQDPTQVQP KQDTQSTTYD ASLDEMSTYN 100
    EISSNQKQQS LSTDDANQNQ TNSVTKNQQE ETNDLTQEDK TSTDTNQLQE 150
    TQSVAKENEK DLGANANNEQ QDKKMTASQP SENQAIETQT ASNDNESQQK 200
    SQQVTSEQNE TATPKVSNTN ASGYNFDYDD EDDDSSTDHL EPISLNNVNA 250
    TSKQTTSYKY KEPAQRVTTN TVKKETASNQ ATIDTKQFTP FSATAQPRTV 300
    YSVSSQKTSS LPKYTPKVNS SINNYIRKKN MKAPRIEEDY TSYFPKYGYR 350
    NGVGRPEGIV VHDTANDNST IDGEIAFMKR NYTNAFVHAF VDGNRIIETA 400
    PTDYLSWGAG PYGNQRFINV EIVHTHDYDS FARSMNNYAD YAATQLQYYN 450
    LKPDSAENDG RGTVWTHAAI SNFLGGTDHA DPHQYLRSHN YSYAELYDLI 500
    YEKYLIKTKQ VAPWGTTSTK PSQPSKPSGG TNNKLTVSAN RGVAQIKPTN 550
    NGLYTTVYDS KGHKTDQVQK TLSVTKTATL GNNKFYLVED YNSGKKYGWV 600
    KQGDVVYNTA KAPVKVNQTY NVKAGSTLYT VPWGTPKQVA SKVSGTGNQT 650
    FKATKQQQID KATYLYGTVN GKSGWISKYY LTTASKPSNP TKPSTNNQLT 700
    VTNNSGVAQI NAKNSGLYTT VYDTKGKTTN QIQRTLSVTK AATLGDKKFY 750
    LVGDYNTGTN YGWVKQDEVI YNTAKSPVKI NQTYNVKPGV KLHTVPWGTY 800
    NQVAGTVSGK GDQTFKATKQ QQIDKATYLY GTVNGKSGWI SKYYLTAPSK 850
    VQALSTQSTP APKQVKPSTQ TVNQIAQVKA NNSGIRASVY DKTAKSGTKY 900
    ANRTFLINKQ RTQGNNTYVL LQDGTSNTPL GWVNINDVTT QNIGKQTQSI 950
    GKYSVKPTNN GLYSIAWGTK NQQLLAPNTL ANQAFNASKA VYVGKDLYLY 1000
    GTVNNRTGWI AAKDLIQNST DAQSTPYNYT FVINNSKSYF YMDPTKANRY 1050
    SLKPYYEQTF TVIKQKNING VKWYYGQLLD GKYVWIKSTD LVKEKIKYAY 1100
    TGMTLNNAIN IQSRLKYKPQ VQNEPLKWSN ANYSQIKNAM DTKRLANDSS 1150
    LKYQFLRLDQ PQYLSAQALN KLLKGKGVLE NQGAAFSQAA RKYGLNEIYL 1200
    ISHALVETGN GTSQLAKGGD VSKGKFTTKT GHKYHNVFGI GAFDNNALVD 1250
    GIKYAKNAGW TSVSKAIIGG AKFIGNSYVK AGQNTLYKMR WNPANPGTHQ 1300
    YATDINWANV NAQVLKQFYD KIGEVGKYFE IPIYK 1335
    Length:1,335
    Mass (Da):148,285
    Last modified:March 1, 2003 - v1
    Checksum:i0D89974CB3DFF0A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015929 Genomic DNA. Translation: AAO04347.1.
    RefSeqiNP_764305.1. NC_004461.1.

    Genome annotation databases

    EnsemblBacteriaiAAO04347; AAO04347; SE_0750.
    GeneIDi1055684.
    KEGGisep:SE0750.
    PATRICi19607295. VBIStaEpi113981_0722.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015929 Genomic DNA. Translation: AAO04347.1 .
    RefSeqi NP_764305.1. NC_004461.1.

    3D structure databases

    ProteinModelPortali Q8CPQ1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 176280.SE0750.

    Protein family/group databases

    CAZyi GH73. Glycoside Hydrolase Family 73.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO04347 ; AAO04347 ; SE_0750 .
    GeneIDi 1055684.
    KEGGi sep:SE0750.
    PATRICi 19607295. VBIStaEpi113981_0722.

    Phylogenomic databases

    eggNOGi COG4193.
    KOi K13714.
    OMAi KSGWISK.
    OrthoDBi EOG6GXTN7.

    Enzyme and pathway databases

    BioCyci SEPI176280:GCDG-755-MONOMER.

    Family and domain databases

    Gene3Di 3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    IPR013338. Lysozyme_subfam2_dom.
    IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
    [Graphical view ]
    Pfami PF01510. Amidase_2. 1 hit.
    PF01832. Glucosaminidase. 1 hit.
    [Graphical view ]
    SMARTi SM00644. Ami_2. 1 hit.
    SM00047. LYZ2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
      Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
      Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 12228.

    Entry informationi

    Entry nameiATL_STAES
    AccessioniPrimary (citable) accession number: Q8CPQ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3