Reviewed, UniProtKB/Swiss-Prot Q8CPP2 (PURK_STAES)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase ATPase subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purK
Ordered Locus Names:	SE_0763

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses an ATPase activity that is dependent on the presence of AIR (aminoimidazole ribonucleotide). The association of purK and purE produces an enzyme complex capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Phosphoribosylaminoimidazole carboxylase ATPase subunit		PRO_0000075010
Regions
	Domain	112 – 296	185	ATP-grasp
	Nucleotide binding	183 – 186	4	ATP By similarity
	Nucleotide binding	266 – 267	2	ATP By similarity
Sites
	Binding site	108	1	ATP By similarity
	Binding site	148	1	ATP By similarity
	Binding site	159	1	ATP By similarity
	Binding site	191	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CPP2-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F8FF4F1BA3995D16	FASTA	375	43,226
	10 20 30 40 50 60 MNFSKLKFGA TIGIIGGGQL GKMMAQSAQK MGYKVIVLDP NEDCPCRYVA HQFIHANYDD 70 80 90 100 110 120 EQALNQLGEN SDVVTYEFEN ISSEQLKKLT QLYHIPQGYQ AIELLQDRLT EKQTLLEANT 130 140 150 160 170 180 QIVPFVQIQT NQDLLKAIEK LGFPFIVKTR FGGYDGKGQI LVRNDSELDE AYQLVEKQEC 190 200 210 220 230 240 VAEQYLDIQK EVSLTVTIGN EQQTTYFPLQ ENEHQNQILF KTVVPARSDK ENEARKEVEK 250 260 270 280 290 300 ITRAIHFVGT FTVEFFIDKE NNLYVNEIAP RPHNSGHYSI EACDYSQFDT HILAITGQKL 310 320 330 340 350 360 PQAIELLKPT VMMNLLGRDL DLLENEFSRH PDWHIHIYGK KERKPDRKMG HMTLLTDDVN 370 QTEQYMLMKF EGRDK

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015929 Genomic DNA. Translation: AAO04360.1.
RefSeq	NP_764318.1.
3D structure databases
HSSP	HSSP built from PDB template 1B6R based on UniProtKB P09029.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CPP2.
Genome annotation databases
GeneID	1057700.
GenomeReviews	Gene locus SE_0763 in contig AE015929_GR.
KEGG	sep:SE0763.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8CPP2.
OMA	RKIGHVN.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_0763-MON.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
TIGRFAMs	TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_STAES

Accession

Primary (citable) accession number: Q8CPP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)