Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Orotidine 5'-phosphate decarboxylase

Gene

pyrF

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10SubstrateUniRule annotation1
Binding sitei31SubstrateUniRule annotation1
Active sitei60Proton donorUniRule annotation1
Binding sitei117SubstrateUniRule annotation1
Binding sitei179SubstrateUniRule annotation1
Binding sitei188SubstrateUniRule annotation1
Binding sitei208Substrate; via amide nitrogenUniRule annotation1
Binding sitei209SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processPyrimidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00070; UER00120

Names & Taxonomyi

Protein namesi
Recommended name:
Orotidine 5'-phosphate decarboxylaseUniRule annotation (EC:4.1.1.23UniRule annotation)
Alternative name(s):
OMP decarboxylaseUniRule annotation
Short name:
OMPDCaseUniRule annotation
Short name:
OMPdecaseUniRule annotation
Gene namesi
Name:pyrFUniRule annotation
Ordered Locus Names:SE_0880
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000001411 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001345801 – 230Orotidine 5'-phosphate decarboxylaseAdd BLAST230

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi176280.SE0880

Structurei

3D structure databases

ProteinModelPortaliQ8CPJ3
SMRiQ8CPJ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 67Substrate bindingUniRule annotation10

Sequence similaritiesi

Belongs to the OMP decarboxylase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106EG9 Bacteria
COG0284 LUCA
KOiK01591

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01200_B OMPdecase_type1_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR014732 OMPdecase
IPR018089 OMPdecase_AS
IPR001754 OMPdeCOase_dom
IPR011060 RibuloseP-bd_barrel
PANTHERiPTHR32119:SF2 PTHR32119:SF2, 1 hit
PfamiView protein in Pfam
PF00215 OMPdecase, 1 hit
SMARTiView protein in SMART
SM00934 OMPdecase, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR01740 pyrF, 1 hit
PROSITEiView protein in PROSITE
PS00156 OMPDECASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8CPJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNLPIIALD FKSADEVHTF LNKFNEPLCV KIGMELFYQT GPALIKSIKK
60 70 80 90 100
RGHDIFLDLK LHDIPNTVSK AMEGLARLDV DLVNVHAAGG IKMMEEAKKG
110 120 130 140 150
LRKHNADIKI IAVTQLTSTT ETQLHEEQNI QTSIEEAVLN YARLTKKAGL
160 170 180 190 200
DGVVCSPLEA EMISKELGTD FLKVTPGIRP KGAARNDQQR ITTPEEAKTL
210 220 230
GSTHIVVGRP ITQSEHPIDS YHKIKESWLS
Length:230
Mass (Da):25,556
Last modified:November 22, 2005 - v2
Checksum:i7AA420D123443B2C
GO

Sequence cautioni

The sequence AAO04477 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA Translation: AAO04477.1 Different initiation.
RefSeqiNP_764435.1, NC_004461.1

Genome annotation databases

EnsemblBacteriaiAAO04477; AAO04477; SE_0880
GeneIDi1057619
KEGGisep:SE0880
PATRICifig|176280.10.peg.852

Similar proteinsi

Entry informationi

Entry nameiPYRF_STAES
AccessioniPrimary (citable) accession number: Q8CPJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: November 22, 2005
Last modified: May 23, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health