Reviewed, UniProtKB/Swiss-Prot Q8CPC2 (ACON_STAES)

Names and origin

Protein names

Recommended name:
    Aconitate hydratase
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase

Gene names

Name:	acnA
Synonyms:	citB
Ordered Locus Names:	SE_1032

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	901 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 901	901	Aconitate hydratase		PRO_0000076672
Sites
	Metal binding	443	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	509	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	512	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CPC2-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E94F6B620AFAA38A	FASTA	901	99,287
	10 20 30 40 50 60 MASNIKEQAK KQFELNGQSY TYYDLQTLEE KGLAKISKLP YSIRVLLESV LRQEDDFVIT 70 80 90 100 110 120 DDHIKALSKF GNAGNEGEVP FKPSRVILQD FTGVPAVVDL ASLRKAMNDV GGDINKINPE 130 140 150 160 170 180 VPVDLVIDHS VQVDSYANPE ALERNMKLEF ERNYERYQFL NWATKAFDNY NAVPPATGIV 190 200 210 220 230 240 HQVNLEYLAN VVHVRDVDGE KTAFPDTLVG TDSHTTMING IGVLGWGVGG IEAEAGMLGQ 250 260 270 280 290 300 PSYFPIPEVI GVRLTHSLPQ GSTATDLALR VTEELRKKGV VGKFVEFFGP GVQHLPLADR 310 320 330 340 350 360 ATIANMAPEY GATCGFFPVD EESLKYMKLT GRDEEHIELV KEYLQQNHMF FDVEKEDPEY 370 380 390 400 410 420 TDVIDLDLST VEASLSGPKR PQDLIFLSDM KKEFEKSVTA PAGNQGHGLD QSEFDKKAEI 430 440 450 460 470 480 NFNDGSKATM KTGDIAIAAI TSCTNTSNPY VMLGAGLVAK KAVEKGLKVP EFVKTSLAPG 490 500 510 520 530 540 SKVVTGYLRD SGLQQYLDDL GFNLVGYGCT TCIGNSGPLL PEIEKAVADE DLLVTSVLSG 550 560 570 580 590 600 NRNFEGRIHP LVKANYLASP QLVVAYALAG TVDIDLQNEP IGKGKDGKDV YLQDIWPSIQ 610 620 630 640 650 660 EVSDTVDKVV TPELFLEEYK NVYHNNEMWN EIDVTDEPLY DFDPNSTYIQ NPTFFQGLSK 670 680 690 700 710 720 EPGKIEPLKS LRVMGKFGDS VTTDHISPAG AIGKDTPAGK YLLDHDVAIR NFNSYGSRRG 730 740 750 760 770 780 NHEVMVRGTF ANIRIKNQLA PGTEGGFTTY WPTGEIMPIY DAAMKYKEDG TGLVVLAGND 790 800 810 820 830 840 YGMGSSRDWA AKGTNLLGVK TVIAQSYERI HRSNLVMMGV LPLQFQQGES AEALGLDGKE 850 860 870 880 890 900 EISVDINEDV QPHDLVNVTA KKENGEIINF KAIVRFDSLV ELDYYRHGGI LQMVLRNKLA Q

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015929 Genomic DNA. Translation: AAO04629.1.
RefSeq	NP_764587.1.
3D structure databases
HSSP	HSSP built from PDB template 7ACN based on UniProtKB P16276.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CPC2.
Genome annotation databases
GeneID	1057349.
GenomeReviews	Gene locus SE_1032 in contig AE015929_GR.
KEGG	sep:SE1032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8CPC2.
OMA	RIKNEML.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_1032-MON.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01341. aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_STAES

Accession

Primary (citable) accession number: Q8CPC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)