ID   HEM2_STAES              Reviewed;         324 AA.
AC   Q8CNZ0;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   16-JUN-2009, entry version 41.
DE   RecName: Full=Delta-aminolevulinic acid dehydratase;
DE            Short=ALADH;
DE            Short=ALAD;
DE            EC=4.2.1.24;
DE   AltName: Full=Porphobilinogen synthase;
GN   Name=hemB; OrderedLocusNames=SE_1343;
OS   Staphylococcus epidermidis (strain ATCC 12228).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=176280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22832016; PubMed=12950922;
RX   DOI=10.1046/j.1365-2958.2003.03671.x;
RA   Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J.,
RA   Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z.,
RA   Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT   "Genome-based analysis of virulence genes in a non-biofilm-forming
RT   Staphylococcus epidermidis strain (ATCC 12228).";
RL   Mol. Microbiol. 49:1577-1593(2003).
CC   -!- CATALYTIC ACTIVITY: 2 5-aminolevulinate = porphobilinogen + 2
CC       H(2)O.
CC   -!- COFACTOR: Zinc (By similarity).
CC   -!- PATHWAY: Porphyrin metabolism; protoporphyrin-IX biosynthesis;
CC       coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
CC   -!- SUBUNIT: Homooctamer (By similarity).
CC   -!- SIMILARITY: Belongs to the ALADH family.
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DR   EMBL; AE015929; AAO04942.1; -; Genomic_DNA.
DR   RefSeq; NP_764898.1; -.
DR   HSSP; Q59643; 1B4K.
DR   GeneID; 1056288; -.
DR   GenomeReviews; AE015929_GR; SE_1343.
DR   KEGG; sep:SE1343; -.
DR   HOGENOM; Q8CNZ0; -.
DR   OMA; Q8CNZ0; AESTPQF.
DR   BioCyc; SEPI176280:SE_1343-MON; -.
DR   GO; GO:0004655; F:porphobilinogen synthase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006779; P:porphyrin biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001731; 4pyrrol_synth_porphobiln_synth.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   PANTHER; PTHR11458; AlaD_dehydratase; 1.
DR   Pfam; PF00490; ALAD; 1.
DR   PIRSF; PIRSF001415; Porphbilin_synth; 1.
DR   PRINTS; PR00144; DALDHYDRTASE.
DR   ProDom; PD002304; AlaD_dehydratase; 1.
DR   PROSITE; PS00169; D_ALA_DEHYDRATASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Lyase; Porphyrin biosynthesis; Zinc.
FT   CHAIN         1    324       Delta-aminolevulinic acid dehydratase.
FT                                /FTId=PRO_0000140517.
FT   REGION      115    133       Zinc-binding (By similarity).
FT   ACT_SITE    248    248       By similarity.
SQ   SEQUENCE   324 AA;  36570 MW;  31685329B5943A51 CRC64;
     MKFDRHRRLR SSKTMRDLVR ETHVRKEDLI YPIFVVEQDD IKSEIKSLPG IYQISLNLLH
     EEIKEAYDLG IRAIMFFGVP NDKDDIGSGA YDHNGVVQEA TRISKNLYKD LLIVADTCLC
     EYTDHGHCGV IDDHTHDVDN DKSLPLLVKT AISQVEAGAD IIAPSNMMDG FVAEIREGLD
     QAGYQNIPIM SYGIKYASSF FGPFRDAADS APSFGDRKTY QMDPANRLEA LRELESDLKE
     GCDMMIVKPS LSYLDIIRDV KNNTNVPVVA YNVSGEYSMT KAAALNGWID EEKIVMEQMI
     SMKRAGADLI ITYFAKDICR YLDK
//