Reviewed, UniProtKB/Swiss-Prot Q8CNX4 (IDH_STAES)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP]
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP

Gene names

Name:	icd
Synonyms:	citC
Ordered Locus Names:	SE_1370

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. Oxalosuccinate + NADP+ = 2-oxoglutarate + CO2 + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Isocitrate dehydrogenase [NADP]		PRO_0000083565
Regions
	Nucleotide binding	344 – 350	7	NADP By similarity
Sites
	Metal binding	310	1	Magnesium or manganese By similarity
	Binding site	94	1	NADP By similarity
	Binding site	103	1	Substrate By similarity
	Binding site	105	1	Substrate By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	143	1	Substrate By similarity
	Binding site	357	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	396	1	NADP By similarity
	Binding site	400	1	NADP By similarity
	Site	150	1	Critical for catalysis By similarity
	Site	220	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CNX4-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 31EA599CF275A83C	FASTA	422	46,619
	10 20 30 40 50 60 MSAEKITQSK DGLNVPNEPI IPFIIGDGIG PDIWKAASRV IDAAVEKAYN GEKRIEWKEV 70 80 90 100 110 120 LAGQKAYDET GEWLPQETLE TIKEYLIAVK GPLTTPIGGG IRSLNVALRQ ELDLFTCLRP 130 140 150 160 170 180 VRWFKGVPSP VKRPEDVDMV IFRENTEDIY AGIEFKQGTS EVKKVIDFLQ NEMGATNIRF 190 200 210 220 230 240 PETSGIGIKP VSKEGTERLV RAAIQYALDN NRKSVTLVHK GNIMKFTEGS FKQWGYDLAH 250 260 270 280 290 300 NEFGDKVFTW QQYDEIVEQK GKDAANEAQS KAEQEGKIII KDSIADIFLQ QILTRPAEHD 310 320 330 340 350 360 VVATMNLNGD YISDALAAQV GGIGIAPGAN INYETGHAIF EATHGTAPKY AGLNKVNPSS 370 380 390 400 410 420 EILSSVLMLE HLGWQEAADK ITDSIEATIA SKIVTYDFAR LMDGAKEVST SDFADELIKN IR

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO04969.1.
RefSeq	NP_764925.1.
3D structure databases
SMR	Q8CNX4. Positions 3-421.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CNX4.
Genome annotation databases
GeneID	1058012.
GenomeReviews	Gene locus SE_1370 in contig AE015929_GR.
KEGG	sep:SE1370.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0538.
HOGENOM	HBG518924.
OMA	RDWGYEL.
PhylomeDB	Q8CNX4.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_1370-MONOMER.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004439. Isocitrate_DH_NADP_prok. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF1. NADP_IDH_prok. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00183. prok_nadp_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH_STAES

Accession

Primary (citable) accession number: Q8CNX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)