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Q8CNU8 (SYL_STAES) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:SE_1431
OrganismStaphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]
Taxonomic identifier176280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAO05030.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152088

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CNU8 [UniParc].

Last modified July 19, 2005. Version 2.
Checksum: 173B55466D9C0725

FASTA80492,164
        10         20         30         40         50         60 
MNYNHKEIEK KWQNYWEENK TFKTNDNLGQ KKFYALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
ISRYKRMQGY NVLHPMGWDA FGLPAEQYAL DTGNDPREFT QKNIQTFKRQ IQELGFSYDW 

       130        140        150        160        170        180 
DREVNTTDPE YYKWTQWIFI QLYNKGLAYV DEVAVNWCPA LGTVLSNEEV VDGVSERGGH 

       190        200        210        220        230        240 
PVYRKPMKQW VLKITEYADR LLEDLDELDW PESIKDMQRN WIGRSEGAKV TFKIEQSDQN 

       250        260        270        280        290        300 
IEVFTTRPDT IYGTSFLVLS PEHPLVNEIT TSDKEQEVKL YQNEASKKSD LERTDLAKEK 

       310        320        330        340        350        360 
TGVFTGTFAI NPLSGDKLPI WIADYVLSTY GTGAVMAVPG HDERDHEFAT KFNLPIIEVI 

       370        380        390        400        410        420 
EGGEVQKYAY TGEGKHINSG ELDGLENEAA ISKAIELLES KGAGEKKVNY KLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPII HWEDGSMTTV PEDELPLLLP ETDEIKPSGT GESPLANIDA FVNVIDEKTG 

       490        500        510        520        530        540 
MKGRRETNTM PQWAGSCWYY LRYIDPHNEK MIADPEKLKH WLPVDLYIGG VEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKVLYD LGVVPTKEPF QKLYNQGMIL GEGNEKMSKS KGNVINPDDI VASHGADTLR 

       610        620        630        640        650        660 
LYEMFMGPLD AAIAWSEKGL DGSRRFLDRV WRLIITDENS INKKIVDSNN HSLDKVYNQT 

       670        680        690        700        710        720 
VKKVTEDFDT LSFNTAISQL MVFINECYKT NEVYKPYIEG FVKMLSPIAP HIGEELWDRL 

       730        740        750        760        770        780 
GHENTITYQP WPTFDESLLV DDEVEIVVQV NGKVRAKINI PKDLSKEEMQ DLALSNDNVK 

       790        800 
MSIEGKEVKK VIAVPQKLVN IVAK 

« Hide

References

[1]"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015929 Genomic DNA. Translation: AAO05030.1. Different initiation.
RefSeqNP_764986.1. NC_004461.1.

3D structure databases

ProteinModelPortalQ8CNU8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176280.SE1431.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO05030; AAO05030; SE_1431.
GeneID1056606.
KEGGsep:SE1431.
PATRIC19608648. VBIStaEpi113981_1397.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMADKPKYYA.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycSEPI176280:GCDG-1437-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_STAES
AccessionPrimary (citable) accession number: Q8CNU8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: July 19, 2005
Last modified: June 11, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries