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Q8CNF8 (LACG_STAES) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
6-phospho-beta-galactosidase
EC=3.2.1.85
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase
Short name=PGALase
P-beta-Gal
Short name=PBG
Gene names
Name:lacG
Ordered Locus Names:SE_1781
OrganismStaphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]
Taxonomic identifier176280 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Staphylococcus ›

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP-Rule MF_01574

Pathway

Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP-Rule MF_01574

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlactose catabolic process via tagatose-6-phosphate

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phospho-beta-galactosidase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4704706-phospho-beta-galactosidase HAMAP-Rule MF_01574
PRO_0000063891

Sites

Active site1601Proton donor By similarity
Active site3751Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CNF8 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: E81C610AB95A77EE

FASTA47054,836
        10         20         30         40         50         60 
MTKKLPDDFI FGGATAAYQA EGATQTDGKG RVAWDTYLEE NYWYTAEPAS DFYNRYPVDL 

        70         80         90        100        110        120 
ELSERFGVNG IRISIAWSRI FPKGYGEVNQ KGVEYYHNLF KECHKRHVEP FVTLHHFDTP 

       130        140        150        160        170        180 
EVLHKDGDFL NRKTIDYFVD YAEFCFKEFP EVKYWTTFNE IGPIGDGQYL VGKFPPGIKY 

       190        200        210        220        230        240 
DFEKVFQSHH NMMVAHARAV KLFKDENYKG EIGVVHALPT KYPYDPSNPE DVRAAELEDI 

       250        260        270        280        290        300 
IHNKFILDAT YLGKYSRETM EGVQHILSVN GGQLEISDED YKILDEAKDL NDFLGINYYM 

       310        320        330        340        350        360 
SDWMRGFEGE SEITHNATGD KGGSKYQLKG VGQREFDVDV PRTDWDWMIY PQGLYDQIMR 

       370        380        390        400        410        420 
VVKDYPNYHK IYITENGLGY KDVFDEKEKT VHDDARIDYI KQHLSVIADA IADGANVKGY 

       430        440        450        460        470 
FLWSLMDVFS WSNGYEKRYG LFYVDFETQE RFPKKSAYWY KELAESKEIK

« Hide

References

[1]"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE015929 Genomic DNA. Translation: AAO05422.1.
RefSeqNP_765336.1. NC_004461.1.

3D structure databases

ProteinModelPortalQ8CNF8.
SMRQ8CNF8. Positions 1-470.
ModBaseSearch...

Protein-protein interaction databases

STRING176280.SE1781.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO05422; AAO05422; SE_1781.
GeneID1057113.
KEGGsep:SE1781.
PATRIC19609436. VBIStaEpi113981_1738.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2723.
KOK01220.
OMAPYSGFPP.
ProtClustDBPRK13511.

Enzyme and pathway databases

UniPathwayUPA00542; UER00605.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
HAMAPMF_01574. LacG.
InterProIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMsTIGR01233. lacG. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLACG_STAES
AccessionPrimary (citable) accession number: Q8CNF8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2003
Last modified: May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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