Reviewed, UniProtKB/Swiss-Prot Q8CNF7 (PTLCB_STAES)

Names and origin

Protein names

Recommended name:
    PTS system lactose-specific EIICB component
Alternative name(s):
    EIICB-Lac
      Short name=EII-Lac
Including the following 2 domains:
    1- Recommended name:
            Lactose permease IIC component
        Alternative name(s):
            PTS system lactose-specific EIIC component
    2- Recommended name:
            Lactose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system lactose-specific EIIB component

Gene names

Name:	lacE
Ordered Locus Names:	SE_1782

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lactose transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-3 domain. Contains 1 PTS EIIC type-3 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 582	582	PTS system lactose-specific EIICB component		PRO_0000186592
Regions
	Transmembrane	29 – 49	21	Potential
	Transmembrane	60 – 80	21	Potential
	Transmembrane	101 – 121	21	Potential
	Transmembrane	130 – 150	21	Potential
	Transmembrane	173 – 193	21	Potential
	Transmembrane	218 – 238	21	Potential
	Transmembrane	277 – 297	21	Potential
	Transmembrane	333 – 353	21	Potential
	Transmembrane	374 – 394	21	Potential
	Domain	479 – 582	104	PTS EIIB type-3
Sites
	Active site	486	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CNF7-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 256AC4A2AC536C1B	FASTA	582	63,765
	10 20 30 40 50 60 MNKLIAWIEK GKPFFEKISR NIYLRAIRDG FIAAIPIILF SSIFILITYV PNVFGFTWSK 70 80 90 100 110 120 TMEGILMKPY NYTMGIVGLL VAGTTAKSLT DSYNRKLDKT NQINFISTMM AAICGFLFLA 130 140 150 160 170 180 ADPVKDGGFS SAFMGTKGLL TAFISAFITV IVYNFFVKRN ITIKMPKEVP PNISQVFKDI 190 200 210 220 230 240 FPLSAVILIL YALDLLSRAI VHTNVANAVL KVFEPLFTAA DGWIGVTLIF GAFAFFWFVG 250 260 270 280 290 300 IHGPSIVEPA IAAITYANLE TNLHLIQAGE HADKVITPGT QMFVATMGGT GATLVVPFMF 310 320 330 340 350 360 MWLTKSKRNK AIGRASVVPT FFGVNEPILF GAPLVLNPVF FIPFIFAPIV NIWIFKFFVD 370 380 390 400 410 420 VLNMNSFSIF LPWTTPGPLG IVMGTGFAFW SFVLAILLIV VDVIIYYPFL KVYDEQVLEE 430 440 450 460 470 480 ELGNKEANNE LKEKVSANFD TKKADAILAT AGASEADTDD TSSVDETTST SSTDTISEQT 490 500 510 520 530 540 NVLVLCAGGG TSGLLANALN KAAEEYEVPV KAAAGGYGAH MDIMKDYQLI ILAPQVASNF 550 560 570 580 EDIKQDTDRL GIKLAKTEGA QYIKLTRDGE AALEFVKQQF NN

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015929 Genomic DNA. Translation: AAO05423.1.
RefSeq	NP_765337.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CNF7.
Genome annotation databases
GeneID	1057114.
GenomeReviews	Gene locus SE_1782 in contig AE015929_GR.
KEGG	sep:SE1782.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8CNF7.
OMA	WSKDIEN.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_1782-MON.
Family and domain databases
InterPro	IPR014350. PTrfase_system_EIIB_3_subgr. IPR013012. PTS_EIIB_3. IPR003352. PTS_EIIC. IPR003501. PTS_IIB_lac. IPR004501. PTS_IIC_lac. IPR004801. PTS_IIC_lac_spec. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00394. lac_pts_IIC. 1 hit. TIGR00410. lacE. 1 hit. TIGR00853. pts-lac. 1 hit.
PROSITE	PS51100. PTS_EIIB_TYPE_3. 1 hit. PS51105. PTS_EIIC_TYPE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTLCB_STAES

Accession

Primary (citable) accession number: Q8CNF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)