Reviewed, UniProtKB/Swiss-Prot Q8CN22 (LDHD_STAES)

Names and origin

Protein names

Recommended name:
    D-lactate dehydrogenase
      Short name=D-LDH
    EC=1.1.1.28
Alternative name(s):
    D-specific D-2-hydroxyacid dehydrogenase

Gene names

Name:	ldhD
Synonyms:	ddh
Ordered Locus Names:	SE_2074

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-lactate + NAD+ = pyruvate + NADH.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	D-lactate dehydrogenase		PRO_0000075966
Regions
	Nucleotide binding	156 – 157	2	NAD By similarity
	Nucleotide binding	233 – 235	3	NAD By similarity
	Nucleotide binding	296 – 299	4	NAD By similarity
Sites
	Active site	235	1	By similarity
	Active site	264	1	By similarity
	Active site	296	1	Proton donor By similarity
	Binding site	259	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CN22-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E49590DB57226722	FASTA	330	36,526
	10 20 30 40 50 60 MTKIMFFGTR AYEKDMALRW GKKNNIDVTT STELLSVDTV DQLKDYDGVT TMQFGKLEPE 70 80 90 100 110 120 VYPKLESYGI KQIAQRTAGF DMYDLELAKK HEIIISNIPS YSPETIAEYS VSIALQLVRK 130 140 150 160 170 180 FPTIEKRVQA HNFTWASPIM SRPVKNMTVA IIGTGRIGAA TGKIYAGFGA RVVGYDAYPN 190 200 210 220 230 240 HSLSFLEYKE TVEDAIKDAD IISLHVPANK DSFHLFDNNM FKNVKKGAVL VNAARGAVIN 250 260 270 280 290 300 TPDLIEAVNN GTLSGAAIDT YENEANYFTF DCSNQTIDDP ILLDLIRNEN ILVTPHIAFF 310 320 330 SDEAVQNLVE GGLNAALSVI NTGTCDTRLN

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO05716.1.
RefSeq	NP_765629.1.
3D structure databases
SMR	Q8CN22. Positions 1-329.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CN22.
Genome annotation databases
GeneID	1057713.
GenomeReviews	Gene locus SE_2074 in contig AE015929_GR.
KEGG	sep:SE2074.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1052.
HOGENOM	HBG731446.
OMA	TWGLPIR.
PhylomeDB	Q8CN22.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_2074-MONOMER.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHD_STAES

Accession

Primary (citable) accession number: Q8CN22

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2003
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)