Q8CMX3 (CYSH_STAES) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase

Gene names

Name:	cysH
Ordered Locus Names:	SE_2181

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_0000100646

Sequences

	Sequence		Length	Mass (Da)
	Q8CMX3 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: BB97CF62DB714A93	FASTA	243	28,338
	10 20 30 40 50 60 MSIERITYDN FQNDPFINEL DINDETKGAY EILKWAYQTY ENDIVYSCSF GAESMVLIDL 70 80 90 100 110 120 ISQIKPDAQI VFLDTDLHFQ ETYDLIDRVK DKYPQLRIKM KKPELTLEEQ GEKYNPALWK 130 140 150 160 170 180 NDPNQCCYIR KIKPLEDVLS GAVAWISGLR RAQSPTRAHT NFINKDERFK SIKVCPLIYW 190 200 210 220 230 240 TEEEVWSYIR DKDLPYNELH DQNYPSIGCI PCTSPVFDSN DSRAGRWSNS SKTECGLHVA DKP

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO05823.1.
RefSeq	NP_765736.1. NC_004461.1.
3D structure databases
ProteinModelPortal	Q8CMX3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CMX3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000041021; EBSTAP00000039654; EBSTAG00000041019.
GeneID	1056387.
GenomeReviews	Gene locus SE_2181 in contig AE015929_GR.
KEGG	sep:SE2181.
PATRIC	19610220. VBIStaEpi113981_2130.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
GeneTree	EBGT00050000026343.
HOGENOM	HBG758022.
OMA	DETKGAY.
PhylomeDB	Q8CMX3.
ProtClustDB	CLSK313238.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_2181-MONOMER.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR011798. APS_reductase. IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMs	TIGR02055. APS_reductase. 1 hit. TIGR00434. CysH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_STAES

Accession

Primary (citable) accession number: Q8CMX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program