Reviewed, UniProtKB/Swiss-Prot Q8CMQ1 (AHPF_STAES)

Names and origin

Protein names

Recommended name:
    Alkyl hydroperoxide reductase subunit F
    EC=1.6.4.-

Gene names

Name:	ahpF
Ordered Locus Names:	SE_2358

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the ahpC protein By similarity.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Domain	Redox-active center
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on NADH or NADPH Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	Alkyl hydroperoxide reductase subunit F		PRO_0000166785
Regions
	Nucleotide binding	207 – 222	16	FAD By similarity
	Nucleotide binding	347 – 361	15	NAD or NADP By similarity
	Nucleotide binding	467 – 477	11	FAD By similarity
Amino acid modifications
	Disulfide bond	335 ↔ 338		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CMQ1-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C95C637261C5F571	FASTA	507	54,670
	10 20 30 40 50 60 MLNADLKQQL QQLLELMEGD VEFVASLGSD DKSNELKELL NEIAEMSAHI TITEKSLKRT 70 80 90 100 110 120 PSFSVNRPGE ETGITFAGIP LGHEFNSLVL AILQVSGRAP KEKQSIIDQI KGLEGPFHFE 130 140 150 160 170 180 TFVSLTCQKC PDVVQALNLM SVINPNITHT MIDGAVFREE SENIMAVPAV FLDGQEFGNG 190 200 210 220 230 240 RMTVQDILTK LGSTQDASEF NDKDPYDVLI VGGGPASGSA AIYTARKGLR TGIVADRIGG 250 260 270 280 290 300 QVNDTAGIEN FITVKETTGS EFSSNLAEHI AQYDIDTMTG IRATNIEKTD SAIRVTLEND 310 320 330 340 350 360 AVLESKTVII STGASWRKLN IPGEDRLINK GVAFCPHCDG PLFENKDVAV IGGGNSGVEA 370 380 390 400 410 420 AIDLAGIVKH VTLFEYASEL KADSVLQERL RSLPNVDIKT SAKTTEVIGD DYVTGISYED 430 440 450 460 470 480 MTTGESQVVN LDGIFVQIGL VPNTSWLQNA VELNERGEVM INRDNATNVP GIFAAGDVTD 490 500 QKNKQIIISM GAGANAALNA FDYIIRN

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015929 Genomic DNA. Translation: AAO06001.1.
RefSeq	NP_765913.1.
3D structure databases
HSSP	HSSP built from PDB template 1FL2 based on UniProtKB P35340.
ModBase	Search...
Genome annotation databases
GeneID	1056715.
GenomeReviews	Gene locus SE_2358 in contig AE015929_GR.
KEGG	sep:SE2358.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8CMQ1.
OMA	Q8CMQ1. ELEGVFV.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_2358-MON.
Family and domain databases
InterPro	IPR012081. Alkyl_hydroperoxide_Rdtase_suF. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PIRSF	PIRSF000238. AhpF. 1 hit.
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03140. AhpF. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AHPF_STAES

Accession

Primary (citable) accession number: Q8CMQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)