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Protein

Arginine biosynthesis bifunctional protein ArgJ

Gene

argJ

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N2-acetylornithine and glutamate.UniRule annotation

Miscellaneous

Some bacteria possess a monofunctional ArgJ, i.e. capable of catalyzing only the fifth step of the arginine biosynthetic pathway.

Catalytic activityi

N2-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate.UniRule annotation
Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Arginine biosynthesis bifunctional protein ArgJ (argJ)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic), the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Arginine biosynthesis bifunctional protein ArgJ (argJ)
  2. Acetylglutamate kinase (argB)
  3. N-acetyl-gamma-glutamyl-phosphate reductase (argC)
  4. Acetylornithine aminotransferase (argD)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei109Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion holeUniRule annotation1
Sitei110Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion holeUniRule annotation1
Binding sitei146SubstrateUniRule annotation1
Binding sitei168SubstrateUniRule annotation1
Active sitei179NucleophileUniRule annotation1
Binding sitei179SubstrateUniRule annotation1
Binding sitei259SubstrateUniRule annotation1
Binding sitei378SubstrateUniRule annotation1
Binding sitei383SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Multifunctional enzyme, Transferase
Biological processAmino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00068; UER00106
UPA00068; UER00111

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine biosynthesis bifunctional protein ArgJUniRule annotation
Cleaved into the following 2 chains:
Arginine biosynthesis bifunctional protein ArgJ alpha chainUniRule annotation
Arginine biosynthesis bifunctional protein ArgJ beta chainUniRule annotation
Including the following 2 domains:
Glutamate N-acetyltransferaseUniRule annotation (EC:2.3.1.35UniRule annotation)
Alternative name(s):
Ornithine acetyltransferaseUniRule annotation
Short name:
OATaseUniRule annotation
Ornithine transacetylaseUniRule annotation
Amino-acid acetyltransferaseUniRule annotation (EC:2.3.1.1UniRule annotation)
Alternative name(s):
N-acetylglutamate synthaseUniRule annotation
Short name:
AGSaseUniRule annotation
Gene namesi
Name:argJUniRule annotation
Ordered Locus Names:SCO1579
ORF Names:SCI35.01c, SCL24.15c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000022471 – 178Arginine biosynthesis bifunctional protein ArgJ alpha chainUniRule annotationAdd BLAST178
ChainiPRO_0000002248179 – 383Arginine biosynthesis bifunctional protein ArgJ beta chainUniRule annotationAdd BLAST205

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei178 – 179Cleavage; by autolysisUniRule annotation2

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PRIDEiQ8CK24

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta chains.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO1579

Structurei

3D structure databases

ProteinModelPortaliQ8CK24
SMRiQ8CK24
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ArgJ family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C5V Bacteria
COG1364 LUCA
HOGENOMiHOG000022797
InParanoidiQ8CK24
KOiK00620
OMAiGMIAPNM
OrthoDBiPOG091H070E
PhylomeDBiQ8CK24

Family and domain databases

CDDicd02152 OAT, 1 hit
Gene3Di3.60.70.12, 1 hit
HAMAPiMF_01106 ArgJ, 1 hit
InterProiView protein in InterPro
IPR002813 Arg_biosynth_ArgJ
IPR016117 ArgJ-like_dom_sf
PANTHERiPTHR23100 PTHR23100, 1 hit
PfamiView protein in Pfam
PF01960 ArgJ, 1 hit
SUPFAMiSSF56266 SSF56266, 1 hit
TIGRFAMsiTIGR00120 ArgJ, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CK24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVTAAKGFT AAGITAGIKE SGSPDLALVV NTGPRRSAAG VFTSNRVKAA
60 70 80 90 100
PVLWSEQVLK SGEVTAVVLN SGGANACTGP KGFQDTHATA EKAADVLGTG
110 120 130 140 150
AGEVAVCSTG LIGVLLPMDK LLPGVEAAAG QLSEHGGEKA AIAIKTTDTV
160 170 180 190 200
HKTSVVTRDG WTVGGMAKGA GMLAPGLATM LVVITTDADL ETEALDRALR
210 220 230 240 250
AATRVTFDRV DSDGCMSTND TVLLLSSGSS GVTPEYDAFA EAVRTVCDDL
260 270 280 290 300
GQQLIRDAEG ASKDIKVEVV NAATEDEAVQ VGRTIARNNL LKCAIHGEDP
310 320 330 340 350
NWGRVLSAIG TTDAAFEPDR LNVAINGVWV CKNGGVGEDR ELVDMRYREV
360 370 380
HIVADLAAGD ATATIWTNDL TADYVHENSA YSS
Length:383
Mass (Da):39,512
Last modified:March 1, 2003 - v1
Checksum:i4A7345A0A7754B3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939109 Genomic DNA Translation: CAD55284.1
RefSeqiNP_733539.1, NC_003888.3
WP_011027859.1, NC_003888.3

Genome annotation databases

EnsemblBacteriaiCAD55284; CAD55284; CAD55284
GeneIDi1097010
KEGGisco:SCO1579
PATRICifig|100226.15.peg.1591

Similar proteinsi

Entry informationi

Entry nameiARGJ_STRCO
AccessioniPrimary (citable) accession number: Q8CK24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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