Reviewed, UniProtKB/Swiss-Prot Q8CJP7 (DXS2_STRCO)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase 2
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase 2
      Short name=DXP synthase 2
      Short name=DXPS 2

Gene names

Name:	dxs2
Ordered Locus Names:	SCO6013
ORF Names:	SC1C3.01, SC7B7.10

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	1-deoxy-D-xylulose-5-phosphate synthase 2 HAMAP MF_00315		PRO_0000189158

Sequences

	Sequence		Length	Mass (Da)
	Q8CJP7-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 3EC51E08AA9516A8	FASTA	642	68,615
	10 20 30 40 50 60 MPLLTRITGP RDLDRLSLEE LTQLAEEIRT FLVDAVSKTG GHLGPNLGVV ELTLALHRVF 70 80 90 100 110 120 ESPKDKVLWD TGHQSYVHKL LTGRQDFSKL KMKGGLSGYP SQGESEHDVI ENSHASTVLG 130 140 150 160 170 180 WADGIAKANQ VMERDDHVVA VIGDGALTGG MAWEALNNIA AAKDRPLVIV VNDNERSYAP 190 200 210 220 230 240 TIGGLANHLA TLRTTDGYER FLARTKEVLE RTPVVGRPLY DTLHGAKKGL KDFIAPQGMF 250 260 270 280 290 300 EDLGLKYVGP IDGHDLEALE SALTRAKRFG GPVIVHCLTE KGRGYQPALQ DEADRFHAVG 310 320 330 340 350 360 KIHPDTGLPI STSGADWTSV FGDEMLKLGK EREDVVAITA AMLQPVGLDK FAKAFPDRVY 370 380 390 400 410 420 DVGIAEQHGA VSAAGLAHGG VHPVFAVYAT FLNRAFDQVL MDVALHKCGV TFVLDRAGVT 430 440 450 460 470 480 GTDGASHNGM WDMSILQVVP GLRLAAPRDA DQVRAQLREA VVVDDAPTVV RFSKGAVGPA 490 500 510 520 530 540 VPAVGRVGGM DVLRAPGTGT PDVLLVSVGA LAPMCLEVAD LLNKQGISTT VVDPRWVKPV 550 560 570 580 590 600 DEAMAPLAER HRVVVTVEDN SRVGGVGSAV AQALRDAGVD VPLRDFGIPP RFLDHASRAE 610 620 630 640 VLAEIGLTAP DIARQVTGLV ARLDGRYDRA GAEVDQVEAA RD

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL939126 Genomic DNA. Translation: CAD55365.1.
RefSeq	NP_733683.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1101455.
GenomeReviews	Gene locus SCO6013 in contig AL645882_GR.
KEGG	sco:SCO6013.
NMPDR	fig|100226.1.peg.5960.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8CJP7.
OMA	QRFPDRY.
Enzyme and pathway databases
BRENDA	2.2.1.7. 1084.
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS2_STRCO

Accession

Primary (citable) accession number: Q8CJP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)