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Protein

Testis-specific H1 histone

Gene

H1fnt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for normal spermatogenesis and male fertility. Required for proper cell restructuring and DNA condensation during the elongation phase of spermiogenesis. Involved in the histone-protamine transition of sperm chromatin and the subsequent production of functional sperm. Binds both double-stranded and single-stranded DNA, ATP and protamine-1.2 Publications

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB

GO - Biological processi

  • chromosome condensation Source: MGI
  • multicellular organism development Source: UniProtKB-KW
  • nucleus organization Source: MGI
  • spermatid nucleus elongation Source: UniProtKB
  • sperm chromatin condensation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, DNA condensation, Spermatogenesis

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific H1 histone
Alternative name(s):
Haploid germ cell-specific nuclear protein 1
Histone H1t2
Gene namesi
Name:H1fnt
Synonyms:Hanp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1917319. H1fnt.

Subcellular locationi

  • Nucleus
  • Chromosome

  • Note: In round and elongating spermatids, specifically localizes to a chromatin domain at the apical pole.

GO - Cellular componenti

  • nuclear chromatin Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Testis-specific H1 histonePRO_0000343415Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei38 – 381PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CJI4.
PRIDEiQ8CJI4.

PTM databases

iPTMnetiQ8CJI4.
PhosphoSiteiQ8CJI4.

Expressioni

Tissue specificityi

Testis-specific. Specifically expressed in haploid germ cells.2 Publications

Developmental stagei

First detected in round spermatids at stage 4 and expression strongly increases through stages 5-8. Localization in the nucleus is highly polar and it is concentrated in a cap-like structure at the inner periphery of the nuclear membrane. Polarized expression persists in steps 9-14 elongating spermatids before rapidly disappearing by stage 15.1 Publication

Gene expression databases

BgeeiENSMUSG00000048077.
GenevisibleiQ8CJI4. MM.

Interactioni

Protein-protein interaction databases

BioGridi213847. 1 interaction.
IntActiQ8CJI4. 1 interaction.
STRINGi10090.ENSMUSP00000127616.

Structurei

3D structure databases

ProteinModelPortaliQ8CJI4.
SMRiQ8CJI4. Positions 41-108.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili241 – 31272Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the histone H1/H5 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IX90. Eukaryota.
ENOG4111BIG. LUCA.
GeneTreeiENSGT00730000111596.
HOVERGENiHBG097311.
InParanoidiQ8CJI4.
OMAiGAELTIQ.
OrthoDBiEOG091G0RLA.
TreeFamiTF338403.

Sequencei

Sequence statusi: Complete.

Q8CJI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAVQPSGE SQGAELTIQI QQPAERALRT PAKRGTQSVL RVSQLLLRAI
60 70 80 90 100
AGHQHLTLDA LKKELGNAGY EVRREISSHH EGKSTRLEKG TLLRVSGSDA
110 120 130 140 150
AGYFRVWKIS KPREKAGQSR LTLGSHSSGK TVLKSPRPLR PRSRRKAAKK
160 170 180 190 200
AREVWRRKAR ALKARSRRVR TRSTSGARSR TRSRASSRAT SRATSRARSR
210 220 230 240 250
ARSRAQSSAR SSARSSAKSS AKSSTRSSAK SWARSKARSR ARSRAKDLVR
260 270 280 290 300
SKAREQAQAR EQARARAREQ AHARARTQDW VRAKAQEFVS AKEQQYVRAK
310 320 330 340 350
EQERAKAREQ VRIGARDEAR IKAKDYNRVR PTKEDTSPRP AEEKSSNSKL
360 370 380 390
REEKGQEPER PVKQTIQKPA LDNAPSIQGK ACTKSFTKSG QPGDTESP
Length:398
Mass (Da):44,400
Last modified:July 1, 2008 - v2
Checksum:i162206F0E2DCFC57
GO

Sequence cautioni

The sequence BAB24570 differs from that shown. Reason: Frameshift at position 317. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161L → R in AAS49491 (PubMed:15710904).Curated
Sequence conflicti264 – 2641R → H in BAC20949 (PubMed:16055721).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016273 mRNA. Translation: BAC20949.1.
AY496853 mRNA. Translation: AAS49491.1.
BC052363 mRNA. Translation: AAH52363.1.
AK006402 mRNA. Translation: BAB24570.1. Frameshift.
CCDSiCCDS49721.1.
RefSeqiNP_081580.2. NM_027304.2.
UniGeneiMm.107718.

Genome annotation databases

EnsembliENSMUST00000060855; ENSMUSP00000127616; ENSMUSG00000048077.
GeneIDi70069.
KEGGimmu:70069.
UCSCiuc007xmd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016273 mRNA. Translation: BAC20949.1.
AY496853 mRNA. Translation: AAS49491.1.
BC052363 mRNA. Translation: AAH52363.1.
AK006402 mRNA. Translation: BAB24570.1. Frameshift.
CCDSiCCDS49721.1.
RefSeqiNP_081580.2. NM_027304.2.
UniGeneiMm.107718.

3D structure databases

ProteinModelPortaliQ8CJI4.
SMRiQ8CJI4. Positions 41-108.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213847. 1 interaction.
IntActiQ8CJI4. 1 interaction.
STRINGi10090.ENSMUSP00000127616.

PTM databases

iPTMnetiQ8CJI4.
PhosphoSiteiQ8CJI4.

Proteomic databases

PaxDbiQ8CJI4.
PRIDEiQ8CJI4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060855; ENSMUSP00000127616; ENSMUSG00000048077.
GeneIDi70069.
KEGGimmu:70069.
UCSCiuc007xmd.2. mouse.

Organism-specific databases

CTDi341567.
MGIiMGI:1917319. H1fnt.

Phylogenomic databases

eggNOGiENOG410IX90. Eukaryota.
ENOG4111BIG. LUCA.
GeneTreeiENSGT00730000111596.
HOVERGENiHBG097311.
InParanoidiQ8CJI4.
OMAiGAELTIQ.
OrthoDBiEOG091G0RLA.
TreeFamiTF338403.

Miscellaneous databases

PROiQ8CJI4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048077.
GenevisibleiQ8CJI4. MM.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiH1FNT_MOUSE
AccessioniPrimary (citable) accession number: Q8CJI4
Secondary accession number(s): Q5GKZ6, Q5RKV2, Q9CVV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: September 7, 2016
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.