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Protein

Testis-specific H1 histone

Gene

H1fnt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Essential for normal spermatogenesis and male fertility. Required for proper cell restructuring and DNA condensation during the elongation phase of spermiogenesis. Involved in the histone-protamine transition of sperm chromatin and the subsequent production of functional sperm. Binds both double-stranded and single-stranded DNA, ATP and protamine-1.2 Publications

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB

GO - Biological processi

  • chromosome condensation Source: MGI
  • multicellular organism development Source: UniProtKB-KW
  • nucleus organization Source: MGI
  • spermatid nucleus elongation Source: UniProtKB
  • sperm chromatin condensation Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processDifferentiation, DNA condensation, Spermatogenesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific H1 histone
Alternative name(s):
Haploid germ cell-specific nuclear protein 1
Histone H1t2
Gene namesi
Name:H1fnt
Synonyms:Hanp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1917319 H1fnt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003434151 – 398Testis-specific H1 histoneAdd BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei38PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CJI4
PRIDEiQ8CJI4

PTM databases

iPTMnetiQ8CJI4
PhosphoSitePlusiQ8CJI4

Expressioni

Tissue specificityi

Testis-specific. Specifically expressed in haploid germ cells.2 Publications

Developmental stagei

First detected in round spermatids at stage 4 and expression strongly increases through stages 5-8. Localization in the nucleus is highly polar and it is concentrated in a cap-like structure at the inner periphery of the nuclear membrane. Polarized expression persists in steps 9-14 elongating spermatids before rapidly disappearing by stage 15.1 Publication

Gene expression databases

BgeeiENSMUSG00000048077
GenevisibleiQ8CJI4 MM

Interactioni

Protein-protein interaction databases

BioGridi213847, 1 interactor
IntActiQ8CJI4, 1 interactor
MINTiQ8CJI4
STRINGi10090.ENSMUSP00000127616

Structurei

3D structure databases

ProteinModelPortaliQ8CJI4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili241 – 312Sequence analysisAdd BLAST72

Sequence similaritiesi

Belongs to the histone H1/H5 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IX90 Eukaryota
ENOG4111BIG LUCA
GeneTreeiENSGT00730000111596
HOVERGENiHBG097311
InParanoidiQ8CJI4
OMAiWRRKARA
OrthoDBiEOG091G0RLA
TreeFamiTF338403

Sequencei

Sequence statusi: Complete.

Q8CJI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAVQPSGE SQGAELTIQI QQPAERALRT PAKRGTQSVL RVSQLLLRAI
60 70 80 90 100
AGHQHLTLDA LKKELGNAGY EVRREISSHH EGKSTRLEKG TLLRVSGSDA
110 120 130 140 150
AGYFRVWKIS KPREKAGQSR LTLGSHSSGK TVLKSPRPLR PRSRRKAAKK
160 170 180 190 200
AREVWRRKAR ALKARSRRVR TRSTSGARSR TRSRASSRAT SRATSRARSR
210 220 230 240 250
ARSRAQSSAR SSARSSAKSS AKSSTRSSAK SWARSKARSR ARSRAKDLVR
260 270 280 290 300
SKAREQAQAR EQARARAREQ AHARARTQDW VRAKAQEFVS AKEQQYVRAK
310 320 330 340 350
EQERAKAREQ VRIGARDEAR IKAKDYNRVR PTKEDTSPRP AEEKSSNSKL
360 370 380 390
REEKGQEPER PVKQTIQKPA LDNAPSIQGK ACTKSFTKSG QPGDTESP
Length:398
Mass (Da):44,400
Last modified:July 1, 2008 - v2
Checksum:i162206F0E2DCFC57
GO

Sequence cautioni

The sequence BAB24570 differs from that shown. Reason: Frameshift at position 317.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16L → R in AAS49491 (PubMed:15710904).Curated1
Sequence conflicti264R → H in BAC20949 (PubMed:16055721).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016273 mRNA Translation: BAC20949.1
AY496853 mRNA Translation: AAS49491.1
BC052363 mRNA Translation: AAH52363.1
AK006402 mRNA Translation: BAB24570.1 Frameshift.
CCDSiCCDS49721.1
RefSeqiNP_081580.2, NM_027304.2
UniGeneiMm.107718

Genome annotation databases

EnsembliENSMUST00000060855; ENSMUSP00000127616; ENSMUSG00000048077
GeneIDi70069
KEGGimmu:70069
UCSCiuc007xmd.2 mouse

Entry informationi

Entry nameiH1FNT_MOUSE
AccessioniPrimary (citable) accession number: Q8CJI4
Secondary accession number(s): Q5GKZ6, Q5RKV2, Q9CVV3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: March 28, 2018
This is version 67 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health