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Q8CJI3 (S12A8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 12 member 8
Alternative name(s):
Cation-chloride cotransporter 9
Gene names
Name:Slc12a8
Synonyms:Ccc9
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length707 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cation/chloride cotransporter that may play a role in the control of keratinocyte proliferation By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the SLC12A transporter family.

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Symport
Transport
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandChloride
Potassium
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpotassium ion transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionsymporter activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8CJI3-1)

Also known as: ccc9a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8CJI3-2)

Also known as: ccc9b;

The sequence of this isoform differs from the canonical sequence as follows:
     354-379: Missing.
     594-611: ALASLLIMFVIQWLYTLA → EPEIHPGANHLGTVPSQG
     612-707: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 707707Solute carrier family 12 member 8
PRO_0000305289

Regions

Transmembrane36 – 5924Helical; Potential
Transmembrane71 – 9222Helical; Potential
Transmembrane98 – 11518Helical; Potential
Transmembrane122 – 14120Helical; Potential
Transmembrane153 – 17220Helical; Potential
Transmembrane184 – 20421Helical; Potential
Transmembrane232 – 25120Helical; Potential
Transmembrane272 – 29524Helical; Potential
Transmembrane307 – 32923Helical; Potential
Transmembrane361 – 38020Helical; Potential
Transmembrane392 – 41221Helical; Potential
Transmembrane579 – 60022Helical; Potential
Transmembrane606 – 62318Helical; Potential

Natural variations

Alternative sequence354 – 37926Missing in isoform 2.
VSP_028337
Alternative sequence594 – 61118ALASL…LYTLA → EPEIHPGANHLGTVPSQG in isoform 2.
VSP_028338
Alternative sequence612 – 70796Missing in isoform 2.
VSP_028339

Experimental info

Sequence conflict4311E → L in BAC20265. Ref.1
Sequence conflict4331L → R in BAC20265. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (ccc9a) [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 96A01BA7A04EDC57

FASTA70777,073
        10         20         30         40         50         60 
MAQRSPQELF HEAAQQGVLA RSQPWWKIQL FMWEPVLFGT WDGVFTSCMI NIFGVVLFLR 

        70         80         90        100        110        120 
TGWLVGNTGV LLGLLLVSFV ILVALITVLS GIGVAEHGWM GSGGVYSMIS SVLGGQMGGT 

       130        140        150        160        170        180 
VGLLYVFGQC VAGAMYLTGF AESISDLLGL GNIWAVRGIS VAVLLALLGI NLAGVKWIIR 

       190        200        210        220        230        240 
LQLLLLSLLA VSTLDFVVGS FTHLDPEHGF IGYSPELLQS NILPEYSPGE SFFTVFGVFF 

       250        260        270        280        290        300 
PAATGVMAGF NMGGDLREPA DQHTPRLPSS CWVSRGFSNI IFAFLLGAVC TREALRSDFL 

       310        320        330        340        350        360 
IAEKVSLVGF LFLLGLYISS LASCMGGLYG APRILQCIAQ DKVIPALAFL GNGKGPNKTP 

       370        380        390        400        410        420 
VAAICLTSLV TMAFVLVGQV NVLAPVVTIN FMLTYIAVDY SYFALSMAHC GLAQSPEPMP 

       430        440        450        460        470        480 
RQGPDALHCS EHLLQDKAPS YGSDTPAGSL SEGTLLEFTK DMDQLLQPIG GLESQPRSRE 

       490        500        510        520        530        540 
VNQVLKSQKH KCKKDAKQTL QDSFLLDAES PLSFSMGASE RLSVASCGEQ ESYQNQQTSR 

       550        560        570        580        590        600 
SESHDHLVPD LCTQPRANGE DFFLKCRLQE QQVQRRPSAF YVCMCNPWVS LLGALASLLI 

       610        620        630        640        650        660 
MFVIQWLYTL ASMGVAALVY LYIGQASPGL YLGSASNFSF FQWMKSFLVP SCRSLRSTRE 

       670        680        690        700 
QIILAPSPAK VDMAMTQAYP GQWQTSATRE RYHRSSFLSR EQLMPPY 

« Hide

Isoform 2 (ccc9b) [UniParc].

Checksum: 6073486F2785D71D
Show »

FASTA58563,375

References

[1]"Molecular cloning of a new member of cation chloride cotransporters."
Ishibashi K., Imai M.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB024430 mRNA. Translation: BAC20264.1.
AB024431 mRNA. Translation: BAC20265.1.
RefSeqNP_705889.1. NM_153625.1. [Q8CJI3-1]
UniGeneRn.15381.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ8CJI3.
PRIDEQ8CJI3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID266733.
KEGGrno:266733.
UCSCRGD:628730. rat. [Q8CJI3-1]

Organism-specific databases

CTD84561.
RGD628730. Slc12a8.

Phylogenomic databases

eggNOGCOG0531.
HOGENOMHOG000045198.
HOVERGENHBG108430.
InParanoidQ8CJI3.
KOK14428.
PhylomeDBQ8CJI3.

Gene expression databases

GenevestigatorQ8CJI3.

Family and domain databases

InterProIPR004841. AA-permease/SLC12A_dom.
[Graphical view]
PfamPF00324. AA_permease. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio624577.

Entry information

Entry nameS12A8_RAT
AccessionPrimary (citable) accession number: Q8CJI3
Secondary accession number(s): Q8CJI2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families