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Protein

Neuronal PAS domain-containing protein 4

Gene

Npas4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor expressed in neurons of the brain that regulates the excitatory-inhibitory balance within neural circuits and is required for contextual memory in the hyppocampus (By similarity). Plays a key role in the structural and functional plasticity of neurons (By similarity). Acts as an early-response transcription factor in both excitatory and inhibitory neurons, where it induces distinct but overlapping sets of late-response genes in these two types of neurons, allowing the synapses that form on inhibitory and excitatory neurons to be modified by neuronal activity in a manner specific to their function within a circuit, thereby facilitating appropriate circuit responses to sensory experience (By similarity). In excitatory neurons, activates transcription of BDNF, which in turn controls the number of GABA-releasing synapses that form on excitatory neurons, thereby promoting an increased number of inhibitory synapses on excitatory neurons (By similarity). In inhibitory neurons, regulates a distinct set of target genes that serve to increase excitatory input onto somatostatin neurons, probably resulting in enhanced feedback inhibition within cortical circuits (By similarity). The excitatory and inhibitory balance in neurons affects a number of processes, such as short-term and long-term memory, acquisition of experience, fear memory, response to stress and social behavior (PubMed:21887312). Acts as a regulator of dendritic spine development in olfactory bulb granule cells in a sensory-experience-dependent manner by regulating expression of MDM2 (By similarity). Efficient DNA binding requires dimerization with another bHLH protein, such as ARNT, ARNT2 or BMAL1 (By similarity). Can activate the CME (CNS midline enhancer) element (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal PAS domain-containing protein 4Curated
Short name:
Neuronal PAS4Curated
Alternative name(s):
HLH-PAS transcription factor NXF1 Publication
Gene namesi
Name:Npas4Imported
Synonyms:Nxf1 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi628866 Npas4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002482241 – 802Neuronal PAS domain-containing protein 4Add BLAST802

Post-translational modificationi

Ubiquitinated, leading to degradation by the proteosome.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ8CJH6
PRIDEiQ8CJH6

Expressioni

Tissue specificityi

Specifically expressed in neurons (PubMed:18815592). Expressed in the lateral nucleus of the amygdala (at protein level) (PubMed:21887312).2 Publications

Inductioni

Expression is regulated by neuronal activity (PubMed:18815592). Induced in excitatory neurons specifically upon calcium influx (PubMed:18815592). Induced in the lateral nucleus of the amygdala in a learning-dependent manner (at protein level) (PubMed:21887312).2 Publications

Gene expression databases

BgeeiENSRNOG00000020009

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Heterodimer; forms a heterodimer with ARNT, ARNT2 or BMAL1.By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027119

Structurei

3D structure databases

ProteinModelPortaliQ8CJH6
SMRiQ8CJH6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 53bHLHPROSITE-ProRule annotationAdd BLAST53
Domaini70 – 144PAS 1PROSITE-ProRule annotationAdd BLAST75
Domaini203 – 275PAS 2PROSITE-ProRule annotationAdd BLAST73
Domaini280 – 319PACAdd BLAST40

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 13Basic motif; degeneratePROSITE-ProRule annotationAdd BLAST13
Regioni14 – 53Helix-loop-helix motifPROSITE-ProRule annotationAdd BLAST40

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili5 – 38Sequence analysisAdd BLAST34
Coiled coili624 – 648Sequence analysisAdd BLAST25

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IISB Eukaryota
ENOG4110GBU LUCA
GeneTreeiENSGT00530000064165
HOGENOMiHOG000113839
HOVERGENiHBG082054
InParanoidiQ8CJH6
OMAiPDPNGEW
OrthoDBiEOG091G0X71
PhylomeDBiQ8CJH6
TreeFamiTF319684

Family and domain databases

CDDicd00130 PAS, 2 hits
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013655 PAS_fold_3
PfamiView protein in Pfam
PF08447 PAS_3, 1 hit
SMARTiView protein in SMART
SM00091 PAS, 2 hits
SUPFAMiSSF55785 SSF55785, 2 hits
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 2 hits

Sequencei

Sequence statusi: Complete.

Q8CJH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRSTKGASK ARRDQINAEI RNLKELLPLA EADKVRLSYL HIMSLACIYT
60 70 80 90 100
RKGVFFAGGT PLAGPTGLLS AQELEDIVAA LPGFLLVFTA EGKLLYLSES
110 120 130 140 150
VSEHLGHSMV DLVAQGDSIY DIIDPADHLT VRQQLTMPSA LDADRLFRCR
160 170 180 190 200
FNTSKSLRRQ SAGNKLVLIR GRFHAHPPGA YWAGNPVFTA FCAPLEPRPR
210 220 230 240 250
PGPGPGPGPG PASLFLAMFQ SRHAKDLALL DISESVLIYL GFERSELLCK
260 270 280 290 300
SWYGLLHPED LAHASSQHYR LLAENGDIQA EMVVRLQAKH GGWTWIYCML
310 320 330 340 350
YSDGPEGPIT ANNYPISDTE AWSLRQQLNS ENTQAAYVLG TPAVLPSFSE
360 370 380 390 400
NVFSQEHCSN PLFTPALGTP RSASFPRAPE LGVISTSEEL AQPSKELDFS
410 420 430 440 450
YLPFPARPEP SLQADLSKDL VCTPPYTPHQ PGGCAFLFSL HEPFQTHLPP
460 470 480 490 500
PSSSLQEQLT PSTVTFSEQL TPSSATFPDP LTSSLQGQLT ESSARSFEEQ
510 520 530 540 550
LTPCTSTFPD QLLPSTATFP EPLGSPTHEQ LTPPSTAFQA HLNSPSQTFP
560 570 580 590 600
EQLSPNPTKT YFAQEGCSFL YEKLPPSPSS PGNGDCTLLA LAQLRGPLSV
610 620 630 640 650
DVPLVPEGLL TPEASPVKQS FFHYTEKEQN EIDRLIQQIS QLAQGMDRPF
660 670 680 690 700
SAEAGTGGLE PLGGLEPLNP NLSLSGAGPP VLSLDLKPWK CQELDFLVDP
710 720 730 740 750
DNLFLEETPV EDIFMDLSTP DPNGEWGSGD PEAEVPGGTL SPCNNLSPED
760 770 780 790 800
HSFLEDLATY ETAFETGVST FPYEGFADEL HQLQSQVQDS FHEDGSGGEP

TF
Length:802
Mass (Da):87,318
Last modified:March 1, 2003 - v1
Checksum:i600AA9FBB6D11E25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050103 mRNA Translation: BAC19832.1
RefSeqiNP_705890.1, NM_153626.1
XP_017444330.1, XM_017588841.1
UniGeneiRn.86654

Genome annotation databases

EnsembliENSRNOT00000027119; ENSRNOP00000027119; ENSRNOG00000020009
GeneIDi266734
KEGGirno:266734
UCSCiRGD:628866 rat

Similar proteinsi

Entry informationi

Entry nameiNPAS4_RAT
AccessioniPrimary (citable) accession number: Q8CJH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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