Plexin-B1 precursor - Mus musculus (Mouse)

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Q8CJH3 (PLXB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 86. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Plexin-B1

Gene names

Name:	Plxnb1
Synonyms:	Kiaa0407

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	2119 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for SEMA4D. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Ref.5
Subunit structure	Monomer, and heterodimer with PLXNB2 after proteolytic processing. Binds RAC1 that has been activated by GTP binding. Interaction with SEMA4D promotes binding of cytoplasmic ligands. Interacts with RRAS, ARHGEF11, ARHGEF12, ERBB2, MET, MST1R, RND1, RHOD, NRP1 and NRP2 By similarity. Binds PLXNA1. Ref.1
Subcellular location	Cell membrane; Single-pass type I membrane protein.
Tissue specificity	Detected in brain, heart, lung, liver, kidney, stomach, testis, uterus and placenta. Ref.1
Post-translational modification	Phosphorylated on tyrosine residues by ERBB2 and MET upon SEMA4D binding By similarity. Proteolytic processing favors heterodimerization with PLXNB2 and SEMA4D binding By similarity.
Sequence similarities	Belongs to the plexin family. Contains 3 IPT/TIG domains. Contains 1 Sema domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Coiled coil Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of osteoblast proliferation Inferred from mutant phenotype PubMed 22019888. Source: BHF-UCL neuron projection morphogenesis Inferred from mutant phenotype Ref.5. Source: UniProtKB ossification involved in bone maturation Inferred from mutant phenotype PubMed 22019888. Source: BHF-UCL positive regulation of Rho GTPase activity Inferred from mutant phenotype Ref.5. Source: UniProtKB positive regulation of axonogenesis Inferred from genetic interaction PubMed 15330859. Source: MGI positive regulation of phosphatidylinositol 3-kinase cascade Inferred from mutant phenotype Ref.5. Source: UniProtKB regulation of cell shape Inferred from mutant phenotype Ref.5. Source: UniProtKB regulation of cytoskeleton organization Inferred from sequence or structural similarity. Source: UniProtKB semaphorin-plexin signaling pathway involved in bone trabecula morphogenesis Inferred from mutant phenotype PubMed 22019888. Source: BHF-UCL
Cellular_component	integral to plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB intracellular Inferred from electronic annotation. Source: InterPro semaphorin receptor complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	semaphorin receptor activity Inferred from mutant phenotype Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

Potential

Chain

20 – 2119

2100

Plexin-B1

PRO_0000024672

Regions

Topological domain

20 – 1474

1455

Extracellular Potential

Transmembrane

1475 – 1495

Helical; Potential

Topological domain

1496 – 2119

624

Cytoplasmic Potential

Domain

20 – 479

460

Sema

Domain

1068 – 1158

IPT/TIG 1

Domain

1160 – 1247

IPT/TIG 2

Domain

1250 – 1359

110

IPT/TIG 3

Coiled coil

1492 – 1523

Potential

Compositional bias

678 – 835

158

Pro-rich

Sites

Site

1799

Important for interaction with RAC1 and RND1 By similarity

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

334

N-linked (GlcNAc...) Potential

Glycosylation

349

N-linked (GlcNAc...) Potential

Glycosylation

543

N-linked (GlcNAc...) Ref.6

Glycosylation

1251

N-linked (GlcNAc...) Ref.6

Glycosylation

1314

N-linked (GlcNAc...) Potential

Disulfide bond

79 ↔ 88

By similarity

Disulfide bond

111 ↔ 119

By similarity

Disulfide bond

252 ↔ 377

By similarity

Disulfide bond

268 ↔ 322

By similarity

Disulfide bond

340 ↔ 364

By similarity

Disulfide bond

482 ↔ 499

By similarity

Disulfide bond

488 ↔ 533

By similarity

Disulfide bond

491 ↔ 508

By similarity

Disulfide bond

502 ↔ 514

By similarity

Disulfide bond

570 ↔ 588

By similarity

Experimental info

Sequence conflict

Q → L in BAC22660. Ref.1

Sequence conflict

1777

P → L in BAC97943. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q8CJH3 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 08FB5ECD2E6DE342
Show »

FASTA

2,119

231,376

        10         20         30         40         50         60 
MSVLGPVLLQ VFWAGCVVTL RSPLPAAFTA NGTHLQHLAR DPTTGTLYVG ATNFLFQLSP 

        70         80         90        100        110        120 
GLQLEAVVST GPVNDSRDCL PPVIPDECPQ AQPTNNPNQL LLVSPEALVV CGSVHQGICE 

       130        140        150        160        170        180 
LRSLGQIRQL LLRPERPGDT QYVAANDPAV STVGLVAQGL VGEPLLFVGR GYTSRGVGGG 

       190        200        210        220        230        240 
IPPITTRALR PPDPQAAFSY EETAKLAVGR LSEYSHHFVS AFVRGASAYF LFLRRDLKAP 

       250        260        270        280        290        300 
SRAFRAYVSR VCLQDQHYYS YVELPLACQG GRYGLIQAAA VATSKEVARG DVLFAAFSSV 

       310        320        330        340        350        360 
APPTVDWPLS ASTGASGTSV LCAFPLDEVD QLANYTRDAC YTREGRAENG TKVADIAYDV 

       370        380        390        400        410        420 
LSDCAQLPVD TPDAFPCGSD HTPSPMVSCV PLEATPILEL PGVQLTAVAV TMEDGHTIAF 

       430        440        450        460        470        480 
LGDSQGQLHR VYLGPGRSAA PYSKQSIQPG SPVNRDLTFD GTFEHLYVAT QTTLVKVPVA 

       490        500        510        520        530        540 
PCAQHLDCDS CLAHRDPYCG WCVLLGRCSR RSECSRDQGP EQWLWSFQPE LGCLRVVAVS 

       550        560        570        580        590        600 
PANISREERR EVFLSVPGLP SLWPGESYFC YFGDQQSPAL LTSSGVMCPS PDPSEAPVLQ 

       610        620        630        640        650        660 
RGADHISVNV ELRFGAVVIA STSLSFYDCV AVTASSPSAP CRACVSSRWG CNWCVWQQLC 

       670        680        690        700        710        720 
THKASCDAGP MVASQQSPLL PLIPPARDEL TPFPPTVPQT TVTPTPNSFP IEPRAPSTAS 

       730        740        750        760        770        780 
DVLPGAKPSR LSLWGPWAGP GPILSPTSTE SPLHEKPLPP DPPTIPGTTV PAPTGLGPST 

       790        800        810        820        830        840 
TPEDLLASYP FPSDAAAVSP AEPGPEALPS MVALDQPPGT VPDTTFPGAP GSMKPVLDWL 

       850        860        870        880        890        900 
TKGGGELPEA DEWMGGDTPA FSTSTLLSGD GDSAEHEGPP APLILLSSLD YQYDTPGLWE 

       910        920        930        940        950        960 
LGEVNQRVSS CPCVETVQGS LLIPVHVERE VQLRGRNLWL FQDGPRSSEC VLELGSREVA 

       970        980        990       1000       1010       1020 
VEAQVECAPP PDVWCHIKCQ QHQFSYEALK PELQVGLFLR WAGGLRVDSA DGLHVVLYDC 

      1030       1040       1050       1060       1070       1080 
SVGHGDCSRC QTAMPQYDCV WCEGERPRCV AREACNEAET VATQCPAPLI HSVDPLTGPI 

      1090       1100       1110       1120       1130       1140 
DGGTRVTIRG SNLGQHVQDV LDMVRVAGVP CAVDAGEYDV SSSLVCITGA SGEEVTGTVA 

      1150       1160       1170       1180       1190       1200 
VEVPGRGHGV SEFSFAYQDP KVHSIFPARG PRAGGTRLTL HGSKLLTGRL EDIRVVVGDQ 

      1210       1220       1230       1240       1250       1260 
PCHLLLEQQS EQLHCETGPY PVPAELPVTV LFGATERRLQ HGQFKYTSDP NVTSVGPSKS 

      1270       1280       1290       1300       1310       1320 
FFSGGREIWV RGQDLDVVQR PRIRVTVVPR QHGQGLAQKQ HVVPEKFEEP CLVNSSHLLM 

      1330       1340       1350       1360       1370       1380 
CRTPALPGPP WDSGVQVEFI LDNMVFDFAA LSPTPFSYEA DPTLRSLNPE DPSTPFRHKP 

      1390       1400       1410       1420       1430       1440 
GSVFSVEGEN LDLAMSKEEV VAMIGDGPCV VKTLTRNHLY CEPPVEQPLP HPHALREAPD 

      1450       1460       1470       1480       1490       1500 
ALPEFTVQMG NLRFSLGHVQ YDGESPVAFP VAAQVGLGVG TSLLALGVII IVLIYRRKSK 

      1510       1520       1530       1540       1550       1560 
QALRDYKKVQ IQLENLESSV RDRCKKEFTD LMTEMTDLTS DLLGSGIPFL DYKVYAERVF 

      1570       1580       1590       1600       1610       1620 
FPGYRESPLH RDLGVPDSRR PTVEQGLGQL SNLLNSKLFL TKFIHTLESQ RTFSARDRAY 

      1630       1640       1650       1660       1670       1680 
VASLLTVALH GKLEYFTDIL RTLLSDLVAQ YVAKNPKLML RRTETVVEKL LTNWMSICLY 

      1690       1700       1710       1720       1730       1740 
TFVRDSVGEP LYMLFRGIKH QVDKGPVDSV TGKAKYTLND NRLLREDVEY RPLTLNALLA 

      1750       1760       1770       1780       1790       1800 
VGPGAGEAQC VPVKVLDCDT ISQAKEKMLD QLYKGVPLAQ RPDSCTLDVE WRSGVAGHLI 

      1810       1820       1830       1840       1850       1860 
LSDEDVTSEL QGLWRRLNTL QHYKVPDGAT VALVPCLTKH ILRENQDYVP GERTPMLEDV 

      1870       1880       1890       1900       1910       1920 
DEGGIRPWHL VKPSDEPEPP RPRRGSLRGG ERERAKAIPE IYLTRLLSMK GTLQKFVDDL 

      1930       1940       1950       1960       1970       1980 
FQVILSTSRP VPLAVKYFFD LLDEQAQQHG ISDQDTIHIW KTNSLPLRFW INIIKNPQFV 

      1990       2000       2010       2020       2030       2040 
FDVQTSDNMD AVLLVIAQTF MDACTLADHK LGRDSPINKL LYARDIPRYK QMVERYYADI 

      2050       2060       2070       2080       2090       2100 
RQTVPASDQE MNSVLAELSR NCSADLGARV ALHELYKYIN KYYDQIITAL EEDGTAQKMQ 

      2110 
LGYRLQQIAA AVENKVTDL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Plexin-A1 and plexin-B1 specifically interact at their cytoplasmic domains." Usui H., Taniguchi M., Yokomizo T., Shimizu T. Biochem. Biophys. Res. Commun. 300:927-931(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH PLXNA1. Tissue: Brain.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 913-2119. Tissue: Embryonic tail.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1885-2119. Tissue: Eye.
[5]	"The semaphorin 4D-plexin-B signalling complex regulates dendritic and axonal complexity in developing neurons via diverse pathways." Vodrazka P., Korostylev A., Hirschberg A., Swiercz J.M., Worzfeld T., Deng S., Fazzari P., Tamagnone L., Offermanns S., Kuner R. Eur. J. Neurosci. 30:1193-1208(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-543 AND ASN-1251, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB072381 mRNA. Translation: BAC22660.1. AC140383 Genomic DNA. No translation available. AC174646 Genomic DNA. No translation available. AK129133 mRNA. Translation: BAC97943.1. BC043322 mRNA. Translation: AAH43322.1.
IPI	IPI00229992.
RefSeq	NP_766363.2. NM_172775.2.
UniGene	Mm.53862.
3D structure databases
ProteinModelPortal	Q8CJH3.
SMR	Q8CJH3. Positions 25-533, 1544-2113.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8CJH3. 1 interaction.
MINT	MINT-1791147.
PTM databases
PhosphoSite	Q8CJH3.
Proteomic databases
PaxDb	Q8CJH3.
PRIDE	Q8CJH3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000072093; ENSMUSP00000071966; ENSMUSG00000053646.
GeneID	235611.
KEGG	mmu:235611.
UCSC	uc009rrz.2. mouse.
Organism-specific databases
CTD	5364.
MGI	MGI:2154238. Plxnb1.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG254546.
GeneTree	ENSGT00600000084313.
HOGENOM	HOG000231376.
HOVERGEN	HBG053404.
InParanoid	Q8CJH3.
KO	K06821.
OMA	MIGDGPC.
OrthoDB	EOG40K7XZ.
Gene expression databases
ArrayExpress	Q8CJH3.
Bgee	Q8CJH3.
CleanEx	MM_PLXNB1.
Genevestigator	Q8CJH3.
GermOnline	ENSMUSG00000053646. Mus musculus.
Family and domain databases
Gene3D	2.130.10.10. 1 hit. 2.60.40.10. 4 hits.
InterPro	IPR015716. CD2_adhesion_molc. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR002909. IPT_TIG_rcpt. IPR003659. Plexin-like. IPR016201. Plexin-like_fold. IPR013548. Plexin_cytoplasmic_RasGAP_dom. IPR002165. Plexin_repeat. IPR008936. Rho_GTPase_activation_prot. IPR001627. Semaphorin/CD100_Ag. IPR015943. WD40/YVTN_repeat-like_dom. [Graphical view]
PANTHER	PTHR22625:SF10. PTHR22625:SF10. 1 hit.
Pfam	PF08337. Plexin_cytopl. 1 hit. PF01437. PSI. 1 hit. PF01403. Sema. 1 hit. PF01833. TIG. 3 hits. [Graphical view]
SMART	SM00429. IPT. 3 hits. SM00423. PSI. 3 hits. SM00630. Sema. 1 hit. [Graphical view]
SUPFAM	SSF81296. Ig_E-set. 3 hits. SSF103575. Plexin-like_fold. 1 hit. SSF48350. Rho_GAP. 1 hit. SSF101912. Sema. 1 hit.
PROSITE	PS51004. SEMA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	382789.
SOURCE	Search...

Entry information

Entry name

PLXB1_MOUSE

Accession

Primary (citable) accession number: Q8CJH3
Secondary accession number(s): E9QKS4, Q6ZQC3, Q80ZZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents