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Q8CJH3

- PLXB1_MOUSE

UniProt

Q8CJH3 - PLXB1_MOUSE

Protein

Plexin-B1

Gene

Plxnb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Receptor for SEMA4D. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei1799 – 17991Important for interaction with RAC1 and RND1By similarity

    GO - Molecular functioni

    1. GTPase activating protein binding Source: BHF-UCL
    2. protein binding Source: IntAct
    3. semaphorin receptor activity Source: UniProtKB

    GO - Biological processi

    1. negative regulation of cell adhesion Source: UniProtKB
    2. negative regulation of osteoblast proliferation Source: BHF-UCL
    3. neuron projection morphogenesis Source: UniProtKB
    4. ossification involved in bone maturation Source: BHF-UCL
    5. positive regulation of axonogenesis Source: MGI
    6. positive regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
    7. positive regulation of Rho GTPase activity Source: UniProtKB
    8. regulation of cell shape Source: UniProtKB
    9. regulation of cytoskeleton organization Source: UniProtKB
    10. semaphorin-plexin signaling pathway Source: UniProtKB
    11. semaphorin-plexin signaling pathway involved in bone trabecula morphogenesis Source: BHF-UCL

    Keywords - Molecular functioni

    Receptor

    Enzyme and pathway databases

    ReactomeiREACT_204733. G alpha (12/13) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Plexin-B1
    Gene namesi
    Name:Plxnb1
    Synonyms:Kiaa0407
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:2154238. Plxnb1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: UniProtKB
    2. intracellular Source: InterPro
    3. semaphorin receptor complex Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 21192100Plexin-B1PRO_0000024672Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi31 – 311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi79 ↔ 88PROSITE-ProRule annotation
    Disulfide bondi111 ↔ 119PROSITE-ProRule annotation
    Disulfide bondi252 ↔ 377PROSITE-ProRule annotation
    Disulfide bondi268 ↔ 322PROSITE-ProRule annotation
    Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi340 ↔ 364PROSITE-ProRule annotation
    Glycosylationi349 – 3491N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi482 ↔ 499PROSITE-ProRule annotation
    Disulfide bondi488 ↔ 533PROSITE-ProRule annotation
    Disulfide bondi491 ↔ 508PROSITE-ProRule annotation
    Disulfide bondi502 ↔ 514PROSITE-ProRule annotation
    Glycosylationi543 – 5431N-linked (GlcNAc...)1 Publication
    Disulfide bondi570 ↔ 588PROSITE-ProRule annotation
    Glycosylationi1251 – 12511N-linked (GlcNAc...)1 Publication
    Glycosylationi1314 – 13141N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Phosphorylated on tyrosine residues by ERBB2 and MET upon SEMA4D binding.By similarity
    Proteolytic processing favors heterodimerization with PLXNB2 and SEMA4D binding.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ8CJH3.
    PaxDbiQ8CJH3.
    PRIDEiQ8CJH3.

    PTM databases

    PhosphoSiteiQ8CJH3.

    Expressioni

    Tissue specificityi

    Detected in brain, heart, lung, liver, kidney, stomach, testis, uterus and placenta.1 Publication

    Gene expression databases

    ArrayExpressiQ8CJH3.
    BgeeiQ8CJH3.
    CleanExiMM_PLXNB1.
    GenevestigatoriQ8CJH3.

    Interactioni

    Subunit structurei

    Monomer, and heterodimer with PLXNB2 after proteolytic processing. Binds RAC1 that has been activated by GTP binding. Interaction with SEMA4D promotes binding of cytoplasmic ligands. Interacts with RRAS, ARHGEF11, ARHGEF12, ERBB2, MET, MST1R, RND1, RHOD, NRP1 and NRP2 By similarity. Binds PLXNA1.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Arhgef11Q68FM75EBI-2637650,EBI-2365869
    Arhgef12Q8R4H22EBI-2637650,EBI-8046267

    Protein-protein interaction databases

    IntActiQ8CJH3. 4 interactions.
    MINTiMINT-1791147.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CJH3.
    SMRiQ8CJH3. Positions 25-533, 1544-2113.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 14741455ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1496 – 2119624CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1475 – 149521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 479460SemaPROSITE-ProRule annotationAdd
    BLAST
    Domaini1068 – 115891IPT/TIG 1Add
    BLAST
    Domaini1160 – 124788IPT/TIG 2Add
    BLAST
    Domaini1250 – 1359110IPT/TIG 3Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1492 – 152332Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi678 – 835158Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the plexin family.Curated
    Contains 3 IPT/TIG domains.Curated
    Contains 1 Sema domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG254546.
    GeneTreeiENSGT00600000084313.
    HOGENOMiHOG000231376.
    HOVERGENiHBG053404.
    InParanoidiQ8CJH3.
    KOiK06821.
    OMAiGVMCPSP.
    OrthoDBiEOG7Q8CM9.
    TreeFamiTF312962.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    2.60.40.10. 4 hits.
    InterProiIPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002909. IPT.
    IPR016201. Plexin-like_fold.
    IPR013548. Plexin_cytoplasmic_RasGAP_dom.
    IPR002165. Plexin_repeat.
    IPR008936. Rho_GTPase_activation_prot.
    IPR001627. Semap_dom.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view]
    PfamiPF08337. Plexin_cytopl. 1 hit.
    PF01437. PSI. 1 hit.
    PF01403. Sema. 1 hit.
    PF01833. TIG. 3 hits.
    [Graphical view]
    SMARTiSM00429. IPT. 3 hits.
    SM00423. PSI. 3 hits.
    SM00630. Sema. 1 hit.
    [Graphical view]
    SUPFAMiSSF101912. SSF101912. 1 hit.
    SSF103575. SSF103575. 1 hit.
    SSF48350. SSF48350. 2 hits.
    SSF81296. SSF81296. 3 hits.
    PROSITEiPS51004. SEMA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8CJH3-1 [UniParc]FASTAAdd to Basket

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    MSVLGPVLLQ VFWAGCVVTL RSPLPAAFTA NGTHLQHLAR DPTTGTLYVG     50
    ATNFLFQLSP GLQLEAVVST GPVNDSRDCL PPVIPDECPQ AQPTNNPNQL 100
    LLVSPEALVV CGSVHQGICE LRSLGQIRQL LLRPERPGDT QYVAANDPAV 150
    STVGLVAQGL VGEPLLFVGR GYTSRGVGGG IPPITTRALR PPDPQAAFSY 200
    EETAKLAVGR LSEYSHHFVS AFVRGASAYF LFLRRDLKAP SRAFRAYVSR 250
    VCLQDQHYYS YVELPLACQG GRYGLIQAAA VATSKEVARG DVLFAAFSSV 300
    APPTVDWPLS ASTGASGTSV LCAFPLDEVD QLANYTRDAC YTREGRAENG 350
    TKVADIAYDV LSDCAQLPVD TPDAFPCGSD HTPSPMVSCV PLEATPILEL 400
    PGVQLTAVAV TMEDGHTIAF LGDSQGQLHR VYLGPGRSAA PYSKQSIQPG 450
    SPVNRDLTFD GTFEHLYVAT QTTLVKVPVA PCAQHLDCDS CLAHRDPYCG 500
    WCVLLGRCSR RSECSRDQGP EQWLWSFQPE LGCLRVVAVS PANISREERR 550
    EVFLSVPGLP SLWPGESYFC YFGDQQSPAL LTSSGVMCPS PDPSEAPVLQ 600
    RGADHISVNV ELRFGAVVIA STSLSFYDCV AVTASSPSAP CRACVSSRWG 650
    CNWCVWQQLC THKASCDAGP MVASQQSPLL PLIPPARDEL TPFPPTVPQT 700
    TVTPTPNSFP IEPRAPSTAS DVLPGAKPSR LSLWGPWAGP GPILSPTSTE 750
    SPLHEKPLPP DPPTIPGTTV PAPTGLGPST TPEDLLASYP FPSDAAAVSP 800
    AEPGPEALPS MVALDQPPGT VPDTTFPGAP GSMKPVLDWL TKGGGELPEA 850
    DEWMGGDTPA FSTSTLLSGD GDSAEHEGPP APLILLSSLD YQYDTPGLWE 900
    LGEVNQRVSS CPCVETVQGS LLIPVHVERE VQLRGRNLWL FQDGPRSSEC 950
    VLELGSREVA VEAQVECAPP PDVWCHIKCQ QHQFSYEALK PELQVGLFLR 1000
    WAGGLRVDSA DGLHVVLYDC SVGHGDCSRC QTAMPQYDCV WCEGERPRCV 1050
    AREACNEAET VATQCPAPLI HSVDPLTGPI DGGTRVTIRG SNLGQHVQDV 1100
    LDMVRVAGVP CAVDAGEYDV SSSLVCITGA SGEEVTGTVA VEVPGRGHGV 1150
    SEFSFAYQDP KVHSIFPARG PRAGGTRLTL HGSKLLTGRL EDIRVVVGDQ 1200
    PCHLLLEQQS EQLHCETGPY PVPAELPVTV LFGATERRLQ HGQFKYTSDP 1250
    NVTSVGPSKS FFSGGREIWV RGQDLDVVQR PRIRVTVVPR QHGQGLAQKQ 1300
    HVVPEKFEEP CLVNSSHLLM CRTPALPGPP WDSGVQVEFI LDNMVFDFAA 1350
    LSPTPFSYEA DPTLRSLNPE DPSTPFRHKP GSVFSVEGEN LDLAMSKEEV 1400
    VAMIGDGPCV VKTLTRNHLY CEPPVEQPLP HPHALREAPD ALPEFTVQMG 1450
    NLRFSLGHVQ YDGESPVAFP VAAQVGLGVG TSLLALGVII IVLIYRRKSK 1500
    QALRDYKKVQ IQLENLESSV RDRCKKEFTD LMTEMTDLTS DLLGSGIPFL 1550
    DYKVYAERVF FPGYRESPLH RDLGVPDSRR PTVEQGLGQL SNLLNSKLFL 1600
    TKFIHTLESQ RTFSARDRAY VASLLTVALH GKLEYFTDIL RTLLSDLVAQ 1650
    YVAKNPKLML RRTETVVEKL LTNWMSICLY TFVRDSVGEP LYMLFRGIKH 1700
    QVDKGPVDSV TGKAKYTLND NRLLREDVEY RPLTLNALLA VGPGAGEAQC 1750
    VPVKVLDCDT ISQAKEKMLD QLYKGVPLAQ RPDSCTLDVE WRSGVAGHLI 1800
    LSDEDVTSEL QGLWRRLNTL QHYKVPDGAT VALVPCLTKH ILRENQDYVP 1850
    GERTPMLEDV DEGGIRPWHL VKPSDEPEPP RPRRGSLRGG ERERAKAIPE 1900
    IYLTRLLSMK GTLQKFVDDL FQVILSTSRP VPLAVKYFFD LLDEQAQQHG 1950
    ISDQDTIHIW KTNSLPLRFW INIIKNPQFV FDVQTSDNMD AVLLVIAQTF 2000
    MDACTLADHK LGRDSPINKL LYARDIPRYK QMVERYYADI RQTVPASDQE 2050
    MNSVLAELSR NCSADLGARV ALHELYKYIN KYYDQIITAL EEDGTAQKMQ 2100
    LGYRLQQIAA AVENKVTDL 2119
    Length:2,119
    Mass (Da):231,376
    Last modified:July 27, 2011 - v2
    Checksum:i08FB5ECD2E6DE342
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti36 – 361Q → L in BAC22660. (PubMed:12559962)Curated
    Sequence conflicti1777 – 17771P → L in BAC97943. (PubMed:14621295)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB072381 mRNA. Translation: BAC22660.1.
    AC140383 Genomic DNA. No translation available.
    AC174646 Genomic DNA. No translation available.
    AK129133 mRNA. Translation: BAC97943.1.
    BC043322 mRNA. Translation: AAH43322.1.
    CCDSiCCDS23546.1.
    RefSeqiNP_766363.2. NM_172775.2.
    XP_006512138.1. XM_006512075.1.
    XP_006512139.1. XM_006512076.1.
    XP_006512140.1. XM_006512077.1.
    XP_006512141.1. XM_006512078.1.
    XP_006512142.1. XM_006512079.1.
    XP_006512143.1. XM_006512080.1.
    UniGeneiMm.53862.

    Genome annotation databases

    EnsembliENSMUST00000072093; ENSMUSP00000071966; ENSMUSG00000053646.
    GeneIDi235611.
    KEGGimmu:235611.
    UCSCiuc009rrz.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB072381 mRNA. Translation: BAC22660.1 .
    AC140383 Genomic DNA. No translation available.
    AC174646 Genomic DNA. No translation available.
    AK129133 mRNA. Translation: BAC97943.1 .
    BC043322 mRNA. Translation: AAH43322.1 .
    CCDSi CCDS23546.1.
    RefSeqi NP_766363.2. NM_172775.2.
    XP_006512138.1. XM_006512075.1.
    XP_006512139.1. XM_006512076.1.
    XP_006512140.1. XM_006512077.1.
    XP_006512141.1. XM_006512078.1.
    XP_006512142.1. XM_006512079.1.
    XP_006512143.1. XM_006512080.1.
    UniGenei Mm.53862.

    3D structure databases

    ProteinModelPortali Q8CJH3.
    SMRi Q8CJH3. Positions 25-533, 1544-2113.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8CJH3. 4 interactions.
    MINTi MINT-1791147.

    PTM databases

    PhosphoSitei Q8CJH3.

    Proteomic databases

    MaxQBi Q8CJH3.
    PaxDbi Q8CJH3.
    PRIDEi Q8CJH3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000072093 ; ENSMUSP00000071966 ; ENSMUSG00000053646 .
    GeneIDi 235611.
    KEGGi mmu:235611.
    UCSCi uc009rrz.2. mouse.

    Organism-specific databases

    CTDi 5364.
    MGIi MGI:2154238. Plxnb1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG254546.
    GeneTreei ENSGT00600000084313.
    HOGENOMi HOG000231376.
    HOVERGENi HBG053404.
    InParanoidi Q8CJH3.
    KOi K06821.
    OMAi GVMCPSP.
    OrthoDBi EOG7Q8CM9.
    TreeFami TF312962.

    Enzyme and pathway databases

    Reactomei REACT_204733. G alpha (12/13) signalling events.

    Miscellaneous databases

    NextBioi 382789.
    PROi Q8CJH3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CJH3.
    Bgeei Q8CJH3.
    CleanExi MM_PLXNB1.
    Genevestigatori Q8CJH3.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    2.60.40.10. 4 hits.
    InterProi IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002909. IPT.
    IPR016201. Plexin-like_fold.
    IPR013548. Plexin_cytoplasmic_RasGAP_dom.
    IPR002165. Plexin_repeat.
    IPR008936. Rho_GTPase_activation_prot.
    IPR001627. Semap_dom.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view ]
    Pfami PF08337. Plexin_cytopl. 1 hit.
    PF01437. PSI. 1 hit.
    PF01403. Sema. 1 hit.
    PF01833. TIG. 3 hits.
    [Graphical view ]
    SMARTi SM00429. IPT. 3 hits.
    SM00423. PSI. 3 hits.
    SM00630. Sema. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101912. SSF101912. 1 hit.
    SSF103575. SSF103575. 1 hit.
    SSF48350. SSF48350. 2 hits.
    SSF81296. SSF81296. 3 hits.
    PROSITEi PS51004. SEMA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Plexin-A1 and plexin-B1 specifically interact at their cytoplasmic domains."
      Usui H., Taniguchi M., Yokomizo T., Shimizu T.
      Biochem. Biophys. Res. Commun. 300:927-931(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH PLXNA1.
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 913-2119.
      Tissue: Embryonic tail.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1885-2119.
      Tissue: Eye.
    5. "The semaphorin 4D-plexin-B signalling complex regulates dendritic and axonal complexity in developing neurons via diverse pathways."
      Vodrazka P., Korostylev A., Hirschberg A., Swiercz J.M., Worzfeld T., Deng S., Fazzari P., Tamagnone L., Offermanns S., Kuner R.
      Eur. J. Neurosci. 30:1193-1208(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-543 AND ASN-1251.

    Entry informationi

    Entry nameiPLXB1_MOUSE
    AccessioniPrimary (citable) accession number: Q8CJH3
    Secondary accession number(s): E9QKS4, Q6ZQC3, Q80ZZ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3