Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8CJG1 (AGO1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein argonaute-1

Short name=Argonaute1
Short name=mAgo1
Alternative name(s):
Argonaute RISC catalytic component 1
Eukaryotic translation initiation factor 2C 1
Short name=eIF-2C 1
Short name=eIF2C 1
Piwi/argonaute family protein meIF2C1
Gene names
Name:Ago1
Synonyms:Eif2c1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length857 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. May also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). Ref.4

Subunit structure

Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs) By similarity. Interacts with LIMD1, WTIP and AJUBA By similarity. Interacts with APOBEC3F, APOBEC3G and APOBEC3H By similarity.

Subcellular location

CytoplasmP-body By similarity.

Sequence similarities

Belongs to the argonaute family. Ago subfamily.

Contains 1 PAZ domain.

Contains 1 Piwi domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Trim32Q8CH722EBI-2291996,EBI-773837

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 857857Protein argonaute-1
PRO_0000194056

Regions

Domain226 – 346121PAZ
Domain515 – 816302Piwi
Region309 – 3146Interaction with guide RNA By similarity
Region522 – 56443Interaction with guide RNA By similarity
Region670 – 6756Impairs access of bound RNA to the active site By similarity
Region708 – 7125Interaction with guide RNA By similarity
Region751 – 7599Interaction with guide RNA By similarity
Region788 – 81326Interaction with guide RNA By similarity

Experimental info

Sequence conflict961K → E in BAC15766. Ref.1
Sequence conflict3091Y → H in BAC15766. Ref.1
Sequence conflict3861Y → C in BAC15766. Ref.1
Sequence conflict5501V → A in BAC15766. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8CJG1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 1DBB524AE7CBAF66

FASTA85797,214
        10         20         30         40         50         60 
MEAGPSGAAA GAYLPPLQQV FQAPRRPGIG TVGKPIKLLA NYFEVDIPKI DVYHYEVDIK 

        70         80         90        100        110        120 
PDKCPRRVNR EVVEYMVQHF KPQIFGDRKP VYDGKKNIYT VTALPIGNER VDFEVTIPGE 

       130        140        150        160        170        180 
GKDRIFKVSI KWLAIVSWRM LHEALVSGQI PVPLESVQAL DVAMRHLASM RYTPVGRSFF 

       190        200        210        220        230        240 
SPPEGYYHPL GGGREVWFGF HQSVRPAMWK MMLNIDVSAT AFYKAQPVIE FMCEVLDIRN 

       250        260        270        280        290        300 
IDEQPKPLTD SQRVRFTKEI KGLKVEVTHC GQMKRKYRVC NVTRRPASHQ TFPLQLESGQ 

       310        320        330        340        350        360 
TVECTVAQYF KQKYNLQLKY PHLPCLQVGQ EQKHTYLPLE VCNIVAGQRC IKKLTDNQTS 

       370        380        390        400        410        420 
TMIKATARSA PDRQEEISRL MKNASYNLDP YIQEFGIKVK DDMTEVTGRV LPAPILQYGG 

       430        440        450        460        470        480 
RNRAIATPNQ GVWDMRGKQF YNGIEIKVWA IACFAPQKQC REEVLKNFTD QLRKISKDAG 

       490        500        510        520        530        540 
MPIQGQPCFC KYAQGADSVE PMFRHLKNTY SGLQLIIVIL PGKTPVYAEV KRVGDTLLGM 

       550        560        570        580        590        600 
ATQCVQVKNV VKTSPQTLSN LCLKINVKLG GINNILVPHQ RSAVFQQPVI FLGADVTHPP 

       610        620        630        640        650        660 
AGDGKKPSIT AVVGSMDAHP SRYCATVRVQ RPRQEIIEDL SYMVRELLIQ FYKSTRFKPT 

       670        680        690        700        710        720 
RIIFYRDGVP EGQLPQILHY ELLAIRDACI KLEKDYQPGI TYIVVQKRHH TRLFCADKNE 

       730        740        750        760        770        780 
RIGKSGNIPA GTTVDTNITH PFEFDFYLCS HAGIQGTSRP SHYYVLWDDN RFTADELQIL 

       790        800        810        820        830        840 
TYQLCHTYVR CTRSVSIPAP AYYARLVAFR ARYHLVDKEH DSGEGSHISG QSNGRDPQAL 

       850 
AKAVQVHQDT LRTMYFA 

« Hide

References

« Hide 'large scale' references
[1]"Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors."
Doi N., Zenno S., Ueda R., Ohki-Hamazaki H., Ui-Tei K., Saigo K.
Curr. Biol. 13:41-46(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Essential and overlapping functions for mammalian Argonautes in microRNA silencing."
Su H., Trombly M.I., Chen J., Wang X.
Genes Dev. 23:304-317(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB081471 mRNA. Translation: BAC15766.1.
AL606935, AL606976 Genomic DNA. Translation: CAM17423.1.
AL606976, AL606935 Genomic DNA. Translation: CAM45874.1.
BC129914 mRNA. Translation: AAI29915.1.
RefSeqNP_700452.2. NM_153403.2.
UniGeneMm.30800.

3D structure databases

ProteinModelPortalQ8CJG1.
SMRQ8CJG1. Positions 17-857.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid231767. 1 interaction.
IntActQ8CJG1. 2 interactions.
MINTMINT-4098243.
STRING10090.ENSMUSP00000095498.

PTM databases

PhosphoSiteQ8CJG1.

Proteomic databases

PaxDbQ8CJG1.
PRIDEQ8CJG1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000097888; ENSMUSP00000095498; ENSMUSG00000041530.
GeneID236511.
KEGGmmu:236511.
UCSCuc008utj.2. mouse.

Organism-specific databases

CTD26523.
MGIMGI:2446630. Ago1.

Phylogenomic databases

eggNOGNOG279895.
GeneTreeENSGT00640000091310.
HOGENOMHOG000116043.
HOVERGENHBG006101.
InParanoidA1L365.
KOK11593.
OMAENDILRT.
OrthoDBEOG7HHWRC.
TreeFamTF101510.

Gene expression databases

ArrayExpressQ8CJG1.
BgeeQ8CJG1.
CleanExMM_EIF2C1.
GenevestigatorQ8CJG1.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
InterProIPR014811. DUF1785.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF08699. DUF1785. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTSM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio382987.
PROQ8CJG1.
SOURCESearch...

Entry information

Entry nameAGO1_MOUSE
AccessionPrimary (citable) accession number: Q8CJG1
Secondary accession number(s): A1L365
Entry history
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Translation initiation factors

List of translation initiation factor entries