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Protein

Protein argonaute-1

Gene

Ago1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. May also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).1 Publication

GO - Molecular functioni

  • core promoter binding Source: MGI
  • double-stranded RNA binding Source: MGI
  • miRNA binding Source: MGI
  • poly(A) RNA binding Source: MGI
  • RNA binding Source: MGI
  • RNA polymerase II core binding Source: MGI
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: MGI
  • single-stranded RNA binding Source: MGI
  • transcription regulatory region DNA binding Source: BHF-UCL

GO - Biological processi

  • cellular response to virus Source: GOC-OWL
  • miRNA loading onto RISC involved in gene silencing by miRNA Source: MGI
  • miRNA mediated inhibition of translation Source: UniProtKB
  • miRNA metabolic process Source: MGI
  • nuclear-transcribed mRNA catabolic process Source: UniProtKB
  • positive regulation of gene expression Source: BHF-UCL
  • positive regulation of NIK/NF-kappaB signaling Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • pre-miRNA processing Source: MGI
  • production of miRNAs involved in gene silencing by miRNA Source: MGI
  • RNA secondary structure unwinding Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor, Ribonucleoprotein

Keywords - Biological processi

RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-426496. Post-transcriptional silencing by small RNAs.
R-MMU-5578749. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute-1
Short name:
Argonaute1
Short name:
mAgo1
Alternative name(s):
Argonaute RISC catalytic component 1
Eukaryotic translation initiation factor 2C 1
Short name:
eIF-2C 1
Short name:
eIF2C 1
Piwi/argonaute family protein meIF2C1
Gene namesi
Name:Ago1
Synonyms:Eif2c1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2446630. Ago1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoplasmic mRNA processing body Source: MGI
  • cytosol Source: MGI
  • intracellular ribonucleoprotein complex Source: UniProtKB
  • micro-ribonucleoprotein complex Source: UniProtKB
  • nucleus Source: MGI
  • polysome Source: UniProtKB
  • RISC complex Source: MGI
  • RISC-loading complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940561 – 857Protein argonaute-1Add BLAST857

Proteomic databases

EPDiQ8CJG1.
MaxQBiQ8CJG1.
PaxDbiQ8CJG1.
PRIDEiQ8CJG1.

PTM databases

iPTMnetiQ8CJG1.
PhosphoSitePlusiQ8CJG1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000041530.
CleanExiMM_EIF2C1.
ExpressionAtlasiQ8CJG1. baseline and differential.
GenevisibleiQ8CJG1. MM.

Interactioni

Subunit structurei

Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs) (By similarity). Interacts with LIMD1, WTIP and AJUBA (By similarity). Interacts with APOBEC3F, APOBEC3G and APOBEC3H (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Trim32Q8CH722EBI-2291996,EBI-773837

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231767. 1 interactor.
IntActiQ8CJG1. 2 interactors.
MINTiMINT-4098243.
STRINGi10090.ENSMUSP00000095498.

Structurei

3D structure databases

ProteinModelPortaliQ8CJG1.
SMRiQ8CJG1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini226 – 346PAZPROSITE-ProRule annotationAdd BLAST121
Domaini515 – 816PiwiPROSITE-ProRule annotationAdd BLAST302

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni309 – 314Interaction with guide RNABy similarity6
Regioni522 – 564Interaction with guide RNABy similarityAdd BLAST43
Regioni670 – 675Impairs access of bound RNA to the active siteBy similarity6
Regioni708 – 712Interaction with guide RNABy similarity5
Regioni751 – 759Interaction with guide RNABy similarity9
Regioni788 – 813Interaction with guide RNABy similarityAdd BLAST26

Sequence similaritiesi

Belongs to the argonaute family. Ago subfamily.Curated
Contains 1 PAZ domain.PROSITE-ProRule annotation
Contains 1 Piwi domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1041. Eukaryota.
ENOG410XP07. LUCA.
GeneTreeiENSGT00760000119148.
HOGENOMiHOG000116043.
HOVERGENiHBG006101.
InParanoidiQ8CJG1.
KOiK11593.
OMAiEREYDIM.
OrthoDBiEOG091G020J.
TreeFamiTF101510.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032473. Argonaute_Mid_dom.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16487. ArgoMid. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM01163. DUF1785. 1 hit.
SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CJG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAGPSGAAA GAYLPPLQQV FQAPRRPGIG TVGKPIKLLA NYFEVDIPKI
60 70 80 90 100
DVYHYEVDIK PDKCPRRVNR EVVEYMVQHF KPQIFGDRKP VYDGKKNIYT
110 120 130 140 150
VTALPIGNER VDFEVTIPGE GKDRIFKVSI KWLAIVSWRM LHEALVSGQI
160 170 180 190 200
PVPLESVQAL DVAMRHLASM RYTPVGRSFF SPPEGYYHPL GGGREVWFGF
210 220 230 240 250
HQSVRPAMWK MMLNIDVSAT AFYKAQPVIE FMCEVLDIRN IDEQPKPLTD
260 270 280 290 300
SQRVRFTKEI KGLKVEVTHC GQMKRKYRVC NVTRRPASHQ TFPLQLESGQ
310 320 330 340 350
TVECTVAQYF KQKYNLQLKY PHLPCLQVGQ EQKHTYLPLE VCNIVAGQRC
360 370 380 390 400
IKKLTDNQTS TMIKATARSA PDRQEEISRL MKNASYNLDP YIQEFGIKVK
410 420 430 440 450
DDMTEVTGRV LPAPILQYGG RNRAIATPNQ GVWDMRGKQF YNGIEIKVWA
460 470 480 490 500
IACFAPQKQC REEVLKNFTD QLRKISKDAG MPIQGQPCFC KYAQGADSVE
510 520 530 540 550
PMFRHLKNTY SGLQLIIVIL PGKTPVYAEV KRVGDTLLGM ATQCVQVKNV
560 570 580 590 600
VKTSPQTLSN LCLKINVKLG GINNILVPHQ RSAVFQQPVI FLGADVTHPP
610 620 630 640 650
AGDGKKPSIT AVVGSMDAHP SRYCATVRVQ RPRQEIIEDL SYMVRELLIQ
660 670 680 690 700
FYKSTRFKPT RIIFYRDGVP EGQLPQILHY ELLAIRDACI KLEKDYQPGI
710 720 730 740 750
TYIVVQKRHH TRLFCADKNE RIGKSGNIPA GTTVDTNITH PFEFDFYLCS
760 770 780 790 800
HAGIQGTSRP SHYYVLWDDN RFTADELQIL TYQLCHTYVR CTRSVSIPAP
810 820 830 840 850
AYYARLVAFR ARYHLVDKEH DSGEGSHISG QSNGRDPQAL AKAVQVHQDT

LRTMYFA
Length:857
Mass (Da):97,214
Last modified:July 27, 2011 - v2
Checksum:i1DBB524AE7CBAF66
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96K → E in BAC15766 (PubMed:12526743).Curated1
Sequence conflicti309Y → H in BAC15766 (PubMed:12526743).Curated1
Sequence conflicti386Y → C in BAC15766 (PubMed:12526743).Curated1
Sequence conflicti550V → A in BAC15766 (PubMed:12526743).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081471 mRNA. Translation: BAC15766.1.
AL606935, AL606976 Genomic DNA. Translation: CAM17423.1.
AL606976, AL606935 Genomic DNA. Translation: CAM45874.1.
BC129914 mRNA. Translation: AAI29915.1.
CCDSiCCDS18653.1.
RefSeqiNP_001304102.1. NM_001317173.1.
NP_700452.2. NM_153403.3.
UniGeneiMm.30800.

Genome annotation databases

EnsembliENSMUST00000097888; ENSMUSP00000095498; ENSMUSG00000041530.
GeneIDi236511.
KEGGimmu:236511.
UCSCiuc008utj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081471 mRNA. Translation: BAC15766.1.
AL606935, AL606976 Genomic DNA. Translation: CAM17423.1.
AL606976, AL606935 Genomic DNA. Translation: CAM45874.1.
BC129914 mRNA. Translation: AAI29915.1.
CCDSiCCDS18653.1.
RefSeqiNP_001304102.1. NM_001317173.1.
NP_700452.2. NM_153403.3.
UniGeneiMm.30800.

3D structure databases

ProteinModelPortaliQ8CJG1.
SMRiQ8CJG1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231767. 1 interactor.
IntActiQ8CJG1. 2 interactors.
MINTiMINT-4098243.
STRINGi10090.ENSMUSP00000095498.

PTM databases

iPTMnetiQ8CJG1.
PhosphoSitePlusiQ8CJG1.

Proteomic databases

EPDiQ8CJG1.
MaxQBiQ8CJG1.
PaxDbiQ8CJG1.
PRIDEiQ8CJG1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097888; ENSMUSP00000095498; ENSMUSG00000041530.
GeneIDi236511.
KEGGimmu:236511.
UCSCiuc008utj.2. mouse.

Organism-specific databases

CTDi26523.
MGIiMGI:2446630. Ago1.

Phylogenomic databases

eggNOGiKOG1041. Eukaryota.
ENOG410XP07. LUCA.
GeneTreeiENSGT00760000119148.
HOGENOMiHOG000116043.
HOVERGENiHBG006101.
InParanoidiQ8CJG1.
KOiK11593.
OMAiEREYDIM.
OrthoDBiEOG091G020J.
TreeFamiTF101510.

Enzyme and pathway databases

ReactomeiR-MMU-426496. Post-transcriptional silencing by small RNAs.
R-MMU-5578749. Transcriptional regulation by small RNAs.

Miscellaneous databases

PROiQ8CJG1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041530.
CleanExiMM_EIF2C1.
ExpressionAtlasiQ8CJG1. baseline and differential.
GenevisibleiQ8CJG1. MM.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032473. Argonaute_Mid_dom.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16487. ArgoMid. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM01163. DUF1785. 1 hit.
SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGO1_MOUSE
AccessioniPrimary (citable) accession number: Q8CJG1
Secondary accession number(s): A1L365
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.