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Q8CJG1

- AGO1_MOUSE

UniProt

Q8CJG1 - AGO1_MOUSE

Protein

Protein argonaute-1

Gene

Ago1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. May also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA binding Source: MGI

    GO - Biological processi

    1. negative regulation of translation involved in gene silencing by miRNA Source: UniProtKB
    2. nuclear-transcribed mRNA catabolic process Source: UniProtKB
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor, Ribonucleoprotein

    Keywords - Biological processi

    RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198208. Post-transcriptional silencing by small RNAs.
    REACT_201975. Small interfering RNA (siRNA) biogenesis.
    REACT_221178. MicroRNA (miRNA) biogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein argonaute-1
    Short name:
    Argonaute1
    Short name:
    mAgo1
    Alternative name(s):
    Argonaute RISC catalytic component 1
    Eukaryotic translation initiation factor 2C 1
    Short name:
    eIF-2C 1
    Short name:
    eIF2C 1
    Piwi/argonaute family protein meIF2C1
    Gene namesi
    Name:Ago1
    Synonyms:Eif2c1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:2446630. Ago1.

    Subcellular locationi

    CytoplasmP-body By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. cytoplasmic mRNA processing body Source: MGI
    3. micro-ribonucleoprotein complex Source: UniProtKB
    4. polysome Source: UniProtKB
    5. ribonucleoprotein complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 857857Protein argonaute-1PRO_0000194056Add
    BLAST

    Proteomic databases

    PaxDbiQ8CJG1.
    PRIDEiQ8CJG1.

    PTM databases

    PhosphoSiteiQ8CJG1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8CJG1.
    BgeeiQ8CJG1.
    CleanExiMM_EIF2C1.
    GenevestigatoriQ8CJG1.

    Interactioni

    Subunit structurei

    Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs) By similarity. Interacts with LIMD1, WTIP and AJUBA By similarity. Interacts with APOBEC3F, APOBEC3G and APOBEC3H By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Trim32Q8CH722EBI-2291996,EBI-773837

    Protein-protein interaction databases

    BioGridi231767. 1 interaction.
    IntActiQ8CJG1. 2 interactions.
    MINTiMINT-4098243.
    STRINGi10090.ENSMUSP00000095498.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CJG1.
    SMRiQ8CJG1. Positions 17-857.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini226 – 346121PAZPROSITE-ProRule annotationAdd
    BLAST
    Domaini515 – 816302PiwiPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni309 – 3146Interaction with guide RNABy similarity
    Regioni522 – 56443Interaction with guide RNABy similarityAdd
    BLAST
    Regioni670 – 6756Impairs access of bound RNA to the active siteBy similarity
    Regioni708 – 7125Interaction with guide RNABy similarity
    Regioni751 – 7599Interaction with guide RNABy similarity
    Regioni788 – 81326Interaction with guide RNABy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the argonaute family. Ago subfamily.Curated
    Contains 1 PAZ domain.PROSITE-ProRule annotation
    Contains 1 Piwi domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG279895.
    GeneTreeiENSGT00640000091310.
    HOGENOMiHOG000116043.
    HOVERGENiHBG006101.
    InParanoidiA1L365.
    KOiK11593.
    OMAiEVTHRAN.
    OrthoDBiEOG7HHWRC.
    TreeFamiTF101510.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    InterProiIPR014811. DUF1785.
    IPR003100. PAZ_dom.
    IPR003165. Piwi.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF08699. DUF1785. 1 hit.
    PF02170. PAZ. 1 hit.
    PF02171. Piwi. 1 hit.
    [Graphical view]
    SMARTiSM00949. PAZ. 1 hit.
    SM00950. Piwi. 1 hit.
    [Graphical view]
    SUPFAMiSSF101690. SSF101690. 1 hit.
    SSF53098. SSF53098. 1 hit.
    PROSITEiPS50821. PAZ. 1 hit.
    PS50822. PIWI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CJG1-1 [UniParc]FASTAAdd to Basket

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    MEAGPSGAAA GAYLPPLQQV FQAPRRPGIG TVGKPIKLLA NYFEVDIPKI    50
    DVYHYEVDIK PDKCPRRVNR EVVEYMVQHF KPQIFGDRKP VYDGKKNIYT 100
    VTALPIGNER VDFEVTIPGE GKDRIFKVSI KWLAIVSWRM LHEALVSGQI 150
    PVPLESVQAL DVAMRHLASM RYTPVGRSFF SPPEGYYHPL GGGREVWFGF 200
    HQSVRPAMWK MMLNIDVSAT AFYKAQPVIE FMCEVLDIRN IDEQPKPLTD 250
    SQRVRFTKEI KGLKVEVTHC GQMKRKYRVC NVTRRPASHQ TFPLQLESGQ 300
    TVECTVAQYF KQKYNLQLKY PHLPCLQVGQ EQKHTYLPLE VCNIVAGQRC 350
    IKKLTDNQTS TMIKATARSA PDRQEEISRL MKNASYNLDP YIQEFGIKVK 400
    DDMTEVTGRV LPAPILQYGG RNRAIATPNQ GVWDMRGKQF YNGIEIKVWA 450
    IACFAPQKQC REEVLKNFTD QLRKISKDAG MPIQGQPCFC KYAQGADSVE 500
    PMFRHLKNTY SGLQLIIVIL PGKTPVYAEV KRVGDTLLGM ATQCVQVKNV 550
    VKTSPQTLSN LCLKINVKLG GINNILVPHQ RSAVFQQPVI FLGADVTHPP 600
    AGDGKKPSIT AVVGSMDAHP SRYCATVRVQ RPRQEIIEDL SYMVRELLIQ 650
    FYKSTRFKPT RIIFYRDGVP EGQLPQILHY ELLAIRDACI KLEKDYQPGI 700
    TYIVVQKRHH TRLFCADKNE RIGKSGNIPA GTTVDTNITH PFEFDFYLCS 750
    HAGIQGTSRP SHYYVLWDDN RFTADELQIL TYQLCHTYVR CTRSVSIPAP 800
    AYYARLVAFR ARYHLVDKEH DSGEGSHISG QSNGRDPQAL AKAVQVHQDT 850
    LRTMYFA 857
    Length:857
    Mass (Da):97,214
    Last modified:July 27, 2011 - v2
    Checksum:i1DBB524AE7CBAF66
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti96 – 961K → E in BAC15766. (PubMed:12526743)Curated
    Sequence conflicti309 – 3091Y → H in BAC15766. (PubMed:12526743)Curated
    Sequence conflicti386 – 3861Y → C in BAC15766. (PubMed:12526743)Curated
    Sequence conflicti550 – 5501V → A in BAC15766. (PubMed:12526743)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB081471 mRNA. Translation: BAC15766.1.
    AL606935, AL606976 Genomic DNA. Translation: CAM17423.1.
    AL606976, AL606935 Genomic DNA. Translation: CAM45874.1.
    BC129914 mRNA. Translation: AAI29915.1.
    CCDSiCCDS18653.1.
    RefSeqiNP_700452.2. NM_153403.2.
    UniGeneiMm.30800.

    Genome annotation databases

    EnsembliENSMUST00000097888; ENSMUSP00000095498; ENSMUSG00000041530.
    GeneIDi236511.
    KEGGimmu:236511.
    UCSCiuc008utj.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB081471 mRNA. Translation: BAC15766.1 .
    AL606935 , AL606976 Genomic DNA. Translation: CAM17423.1 .
    AL606976 , AL606935 Genomic DNA. Translation: CAM45874.1 .
    BC129914 mRNA. Translation: AAI29915.1 .
    CCDSi CCDS18653.1.
    RefSeqi NP_700452.2. NM_153403.2.
    UniGenei Mm.30800.

    3D structure databases

    ProteinModelPortali Q8CJG1.
    SMRi Q8CJG1. Positions 17-857.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 231767. 1 interaction.
    IntActi Q8CJG1. 2 interactions.
    MINTi MINT-4098243.
    STRINGi 10090.ENSMUSP00000095498.

    PTM databases

    PhosphoSitei Q8CJG1.

    Proteomic databases

    PaxDbi Q8CJG1.
    PRIDEi Q8CJG1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000097888 ; ENSMUSP00000095498 ; ENSMUSG00000041530 .
    GeneIDi 236511.
    KEGGi mmu:236511.
    UCSCi uc008utj.2. mouse.

    Organism-specific databases

    CTDi 26523.
    MGIi MGI:2446630. Ago1.

    Phylogenomic databases

    eggNOGi NOG279895.
    GeneTreei ENSGT00640000091310.
    HOGENOMi HOG000116043.
    HOVERGENi HBG006101.
    InParanoidi A1L365.
    KOi K11593.
    OMAi EVTHRAN.
    OrthoDBi EOG7HHWRC.
    TreeFami TF101510.

    Enzyme and pathway databases

    Reactomei REACT_198208. Post-transcriptional silencing by small RNAs.
    REACT_201975. Small interfering RNA (siRNA) biogenesis.
    REACT_221178. MicroRNA (miRNA) biogenesis.

    Miscellaneous databases

    NextBioi 382987.
    PROi Q8CJG1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CJG1.
    Bgeei Q8CJG1.
    CleanExi MM_EIF2C1.
    Genevestigatori Q8CJG1.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    InterProi IPR014811. DUF1785.
    IPR003100. PAZ_dom.
    IPR003165. Piwi.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF08699. DUF1785. 1 hit.
    PF02170. PAZ. 1 hit.
    PF02171. Piwi. 1 hit.
    [Graphical view ]
    SMARTi SM00949. PAZ. 1 hit.
    SM00950. Piwi. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101690. SSF101690. 1 hit.
    SSF53098. SSF53098. 1 hit.
    PROSITEi PS50821. PAZ. 1 hit.
    PS50822. PIWI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors."
      Doi N., Zenno S., Ueda R., Ohki-Hamazaki H., Ui-Tei K., Saigo K.
      Curr. Biol. 13:41-46(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Essential and overlapping functions for mammalian Argonautes in microRNA silencing."
      Su H., Trombly M.I., Chen J., Wang X.
      Genes Dev. 23:304-317(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiAGO1_MOUSE
    AccessioniPrimary (citable) accession number: Q8CJG1
    Secondary accession number(s): A1L365
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 14, 2003
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Translation initiation factors
      List of translation initiation factor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3