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Q8CJG1

- AGO1_MOUSE

UniProt

Q8CJG1 - AGO1_MOUSE

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Protein

Protein argonaute-1

Gene

Ago1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. May also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).1 Publication

GO - Molecular functioni

  1. miRNA binding Source: MGI
  2. poly(A) RNA binding Source: Ensembl
  3. RNA binding Source: MGI
  4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: MGI

GO - Biological processi

  1. miRNA metabolic process Source: MGI
  2. negative regulation of translation involved in gene silencing by miRNA Source: UniProtKB
  3. nuclear-transcribed mRNA catabolic process Source: UniProtKB
  4. positive regulation of gene expression Source: MGI
  5. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor, Ribonucleoprotein

Keywords - Biological processi

RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiREACT_198208. Post-transcriptional silencing by small RNAs.
REACT_201975. Small interfering RNA (siRNA) biogenesis.
REACT_221178. MicroRNA (miRNA) biogenesis.
REACT_270446. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute-1
Short name:
Argonaute1
Short name:
mAgo1
Alternative name(s):
Argonaute RISC catalytic component 1
Eukaryotic translation initiation factor 2C 1
Short name:
eIF-2C 1
Short name:
eIF2C 1
Piwi/argonaute family protein meIF2C1
Gene namesi
Name:Ago1
Synonyms:Eif2c1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:2446630. Ago1.

Subcellular locationi

CytoplasmP-body By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytoplasmic mRNA processing body Source: MGI
  3. cytosol Source: MGI
  4. micro-ribonucleoprotein complex Source: UniProtKB
  5. nucleus Source: MGI
  6. polysome Source: UniProtKB
  7. ribonucleoprotein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 857857Protein argonaute-1PRO_0000194056Add
BLAST

Proteomic databases

MaxQBiQ8CJG1.
PaxDbiQ8CJG1.
PRIDEiQ8CJG1.

PTM databases

PhosphoSiteiQ8CJG1.

Expressioni

Gene expression databases

BgeeiQ8CJG1.
CleanExiMM_EIF2C1.
ExpressionAtlasiQ8CJG1. baseline and differential.
GenevestigatoriQ8CJG1.

Interactioni

Subunit structurei

Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs) (By similarity). Interacts with LIMD1, WTIP and AJUBA (By similarity). Interacts with APOBEC3F, APOBEC3G and APOBEC3H (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Trim32Q8CH722EBI-2291996,EBI-773837

Protein-protein interaction databases

BioGridi231767. 1 interaction.
IntActiQ8CJG1. 2 interactions.
MINTiMINT-4098243.
STRINGi10090.ENSMUSP00000095498.

Structurei

3D structure databases

ProteinModelPortaliQ8CJG1.
SMRiQ8CJG1. Positions 17-857.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini226 – 346121PAZPROSITE-ProRule annotationAdd
BLAST
Domaini515 – 816302PiwiPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni309 – 3146Interaction with guide RNABy similarity
Regioni522 – 56443Interaction with guide RNABy similarityAdd
BLAST
Regioni670 – 6756Impairs access of bound RNA to the active siteBy similarity
Regioni708 – 7125Interaction with guide RNABy similarity
Regioni751 – 7599Interaction with guide RNABy similarity
Regioni788 – 81326Interaction with guide RNABy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the argonaute family. Ago subfamily.Curated
Contains 1 PAZ domain.PROSITE-ProRule annotation
Contains 1 Piwi domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG279895.
GeneTreeiENSGT00760000119148.
HOGENOMiHOG000116043.
HOVERGENiHBG006101.
InParanoidiQ8CJG1.
KOiK11593.
OMAiEVTHRAN.
OrthoDBiEOG7HHWRC.
TreeFamiTF101510.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR014811. DUF1785.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. DUF1785. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CJG1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEAGPSGAAA GAYLPPLQQV FQAPRRPGIG TVGKPIKLLA NYFEVDIPKI
60 70 80 90 100
DVYHYEVDIK PDKCPRRVNR EVVEYMVQHF KPQIFGDRKP VYDGKKNIYT
110 120 130 140 150
VTALPIGNER VDFEVTIPGE GKDRIFKVSI KWLAIVSWRM LHEALVSGQI
160 170 180 190 200
PVPLESVQAL DVAMRHLASM RYTPVGRSFF SPPEGYYHPL GGGREVWFGF
210 220 230 240 250
HQSVRPAMWK MMLNIDVSAT AFYKAQPVIE FMCEVLDIRN IDEQPKPLTD
260 270 280 290 300
SQRVRFTKEI KGLKVEVTHC GQMKRKYRVC NVTRRPASHQ TFPLQLESGQ
310 320 330 340 350
TVECTVAQYF KQKYNLQLKY PHLPCLQVGQ EQKHTYLPLE VCNIVAGQRC
360 370 380 390 400
IKKLTDNQTS TMIKATARSA PDRQEEISRL MKNASYNLDP YIQEFGIKVK
410 420 430 440 450
DDMTEVTGRV LPAPILQYGG RNRAIATPNQ GVWDMRGKQF YNGIEIKVWA
460 470 480 490 500
IACFAPQKQC REEVLKNFTD QLRKISKDAG MPIQGQPCFC KYAQGADSVE
510 520 530 540 550
PMFRHLKNTY SGLQLIIVIL PGKTPVYAEV KRVGDTLLGM ATQCVQVKNV
560 570 580 590 600
VKTSPQTLSN LCLKINVKLG GINNILVPHQ RSAVFQQPVI FLGADVTHPP
610 620 630 640 650
AGDGKKPSIT AVVGSMDAHP SRYCATVRVQ RPRQEIIEDL SYMVRELLIQ
660 670 680 690 700
FYKSTRFKPT RIIFYRDGVP EGQLPQILHY ELLAIRDACI KLEKDYQPGI
710 720 730 740 750
TYIVVQKRHH TRLFCADKNE RIGKSGNIPA GTTVDTNITH PFEFDFYLCS
760 770 780 790 800
HAGIQGTSRP SHYYVLWDDN RFTADELQIL TYQLCHTYVR CTRSVSIPAP
810 820 830 840 850
AYYARLVAFR ARYHLVDKEH DSGEGSHISG QSNGRDPQAL AKAVQVHQDT

LRTMYFA
Length:857
Mass (Da):97,214
Last modified:July 27, 2011 - v2
Checksum:i1DBB524AE7CBAF66
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961K → E in BAC15766. (PubMed:12526743)Curated
Sequence conflicti309 – 3091Y → H in BAC15766. (PubMed:12526743)Curated
Sequence conflicti386 – 3861Y → C in BAC15766. (PubMed:12526743)Curated
Sequence conflicti550 – 5501V → A in BAC15766. (PubMed:12526743)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081471 mRNA. Translation: BAC15766.1.
AL606935, AL606976 Genomic DNA. Translation: CAM17423.1.
AL606976, AL606935 Genomic DNA. Translation: CAM45874.1.
BC129914 mRNA. Translation: AAI29915.1.
CCDSiCCDS18653.1.
RefSeqiNP_700452.2. NM_153403.2.
UniGeneiMm.30800.

Genome annotation databases

EnsembliENSMUST00000097888; ENSMUSP00000095498; ENSMUSG00000041530.
GeneIDi236511.
KEGGimmu:236511.
UCSCiuc008utj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081471 mRNA. Translation: BAC15766.1 .
AL606935 , AL606976 Genomic DNA. Translation: CAM17423.1 .
AL606976 , AL606935 Genomic DNA. Translation: CAM45874.1 .
BC129914 mRNA. Translation: AAI29915.1 .
CCDSi CCDS18653.1.
RefSeqi NP_700452.2. NM_153403.2.
UniGenei Mm.30800.

3D structure databases

ProteinModelPortali Q8CJG1.
SMRi Q8CJG1. Positions 17-857.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 231767. 1 interaction.
IntActi Q8CJG1. 2 interactions.
MINTi MINT-4098243.
STRINGi 10090.ENSMUSP00000095498.

PTM databases

PhosphoSitei Q8CJG1.

Proteomic databases

MaxQBi Q8CJG1.
PaxDbi Q8CJG1.
PRIDEi Q8CJG1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000097888 ; ENSMUSP00000095498 ; ENSMUSG00000041530 .
GeneIDi 236511.
KEGGi mmu:236511.
UCSCi uc008utj.2. mouse.

Organism-specific databases

CTDi 26523.
MGIi MGI:2446630. Ago1.

Phylogenomic databases

eggNOGi NOG279895.
GeneTreei ENSGT00760000119148.
HOGENOMi HOG000116043.
HOVERGENi HBG006101.
InParanoidi Q8CJG1.
KOi K11593.
OMAi EVTHRAN.
OrthoDBi EOG7HHWRC.
TreeFami TF101510.

Enzyme and pathway databases

Reactomei REACT_198208. Post-transcriptional silencing by small RNAs.
REACT_201975. Small interfering RNA (siRNA) biogenesis.
REACT_221178. MicroRNA (miRNA) biogenesis.
REACT_270446. Transcriptional regulation by small RNAs.

Miscellaneous databases

NextBioi 382987.
PROi Q8CJG1.
SOURCEi Search...

Gene expression databases

Bgeei Q8CJG1.
CleanExi MM_EIF2C1.
ExpressionAtlasi Q8CJG1. baseline and differential.
Genevestigatori Q8CJG1.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
InterProi IPR014811. DUF1785.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF08699. DUF1785. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view ]
SMARTi SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view ]
SUPFAMi SSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEi PS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors."
    Doi N., Zenno S., Ueda R., Ohki-Hamazaki H., Ui-Tei K., Saigo K.
    Curr. Biol. 13:41-46(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Essential and overlapping functions for mammalian Argonautes in microRNA silencing."
    Su H., Trombly M.I., Chen J., Wang X.
    Genes Dev. 23:304-317(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAGO1_MOUSE
AccessioniPrimary (citable) accession number: Q8CJG1
Secondary accession number(s): A1L365
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3