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Protein

Protein argonaute-1

Gene

Ago1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. May also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).1 Publication

GO - Molecular functioni

  • core promoter binding Source: MGI
  • double-stranded RNA binding Source: MGI
  • miRNA binding Source: MGI
  • RNA binding Source: MGI
  • RNA polymerase II core binding Source: MGI
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
  • single-stranded RNA binding Source: MGI
  • transcription regulatory region DNA binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionRepressor, Ribonucleoprotein, RNA-binding
Biological processRNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-203927 MicroRNA (miRNA) biogenesis
R-MMU-426486 Small interfering RNA (siRNA) biogenesis
R-MMU-426496 Post-transcriptional silencing by small RNAs
R-MMU-5578749 Transcriptional regulation by small RNAs

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute-1
Short name:
Argonaute1
Short name:
mAgo1
Alternative name(s):
Argonaute RISC catalytic component 1
Eukaryotic translation initiation factor 2C 1
Short name:
eIF-2C 1
Short name:
eIF2C 1
Piwi/argonaute family protein meIF2C1
Gene namesi
Name:Ago1
Synonyms:Eif2c1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2446630 Ago1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940561 – 857Protein argonaute-1Add BLAST857

Proteomic databases

EPDiQ8CJG1
MaxQBiQ8CJG1
PaxDbiQ8CJG1
PRIDEiQ8CJG1

PTM databases

iPTMnetiQ8CJG1
PhosphoSitePlusiQ8CJG1

Expressioni

Gene expression databases

BgeeiENSMUSG00000041530
CleanExiMM_EIF2C1
ExpressionAtlasiQ8CJG1 baseline and differential
GenevisibleiQ8CJG1 MM

Interactioni

Subunit structurei

Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs) (By similarity). Interacts with LIMD1, WTIP and AJUBA (By similarity). Interacts with APOBEC3F, APOBEC3G and APOBEC3H (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Trim32Q8CH722EBI-2291996,EBI-773837

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231767, 5 interactors
IntActiQ8CJG1, 5 interactors
MINTiQ8CJG1
STRINGi10090.ENSMUSP00000095498

Structurei

3D structure databases

ProteinModelPortaliQ8CJG1
SMRiQ8CJG1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini226 – 346PAZPROSITE-ProRule annotationAdd BLAST121
Domaini515 – 816PiwiPROSITE-ProRule annotationAdd BLAST302

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni309 – 314Interaction with guide RNABy similarity6
Regioni522 – 564Interaction with guide RNABy similarityAdd BLAST43
Regioni670 – 675Impairs access of bound RNA to the active siteBy similarity6
Regioni708 – 712Interaction with guide RNABy similarity5
Regioni751 – 759Interaction with guide RNABy similarity9
Regioni788 – 813Interaction with guide RNABy similarityAdd BLAST26

Sequence similaritiesi

Belongs to the argonaute family. Ago subfamily.Curated

Phylogenomic databases

eggNOGiKOG1041 Eukaryota
ENOG410XP07 LUCA
GeneTreeiENSGT00760000119148
HOGENOMiHOG000116043
HOVERGENiHBG006101
InParanoidiQ8CJG1
KOiK11593
OMAiTNPNNIE
OrthoDBiEOG091G020J
TreeFamiTF101510

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR014811 ArgoL1
IPR032472 ArgoL2
IPR032473 Argonaute_Mid_dom
IPR032474 Argonaute_N
IPR003100 PAZ_dom
IPR036085 PAZ_dom_sf
IPR003165 Piwi
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF08699 ArgoL1, 1 hit
PF16488 ArgoL2, 1 hit
PF16487 ArgoMid, 1 hit
PF16486 ArgoN, 1 hit
PF02170 PAZ, 1 hit
PF02171 Piwi, 1 hit
SMARTiView protein in SMART
SM01163 DUF1785, 1 hit
SM00949 PAZ, 1 hit
SM00950 Piwi, 1 hit
SUPFAMiSSF101690 SSF101690, 1 hit
SSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS50821 PAZ, 1 hit
PS50822 PIWI, 1 hit

Sequencei

Sequence statusi: Complete.

Q8CJG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAGPSGAAA GAYLPPLQQV FQAPRRPGIG TVGKPIKLLA NYFEVDIPKI
60 70 80 90 100
DVYHYEVDIK PDKCPRRVNR EVVEYMVQHF KPQIFGDRKP VYDGKKNIYT
110 120 130 140 150
VTALPIGNER VDFEVTIPGE GKDRIFKVSI KWLAIVSWRM LHEALVSGQI
160 170 180 190 200
PVPLESVQAL DVAMRHLASM RYTPVGRSFF SPPEGYYHPL GGGREVWFGF
210 220 230 240 250
HQSVRPAMWK MMLNIDVSAT AFYKAQPVIE FMCEVLDIRN IDEQPKPLTD
260 270 280 290 300
SQRVRFTKEI KGLKVEVTHC GQMKRKYRVC NVTRRPASHQ TFPLQLESGQ
310 320 330 340 350
TVECTVAQYF KQKYNLQLKY PHLPCLQVGQ EQKHTYLPLE VCNIVAGQRC
360 370 380 390 400
IKKLTDNQTS TMIKATARSA PDRQEEISRL MKNASYNLDP YIQEFGIKVK
410 420 430 440 450
DDMTEVTGRV LPAPILQYGG RNRAIATPNQ GVWDMRGKQF YNGIEIKVWA
460 470 480 490 500
IACFAPQKQC REEVLKNFTD QLRKISKDAG MPIQGQPCFC KYAQGADSVE
510 520 530 540 550
PMFRHLKNTY SGLQLIIVIL PGKTPVYAEV KRVGDTLLGM ATQCVQVKNV
560 570 580 590 600
VKTSPQTLSN LCLKINVKLG GINNILVPHQ RSAVFQQPVI FLGADVTHPP
610 620 630 640 650
AGDGKKPSIT AVVGSMDAHP SRYCATVRVQ RPRQEIIEDL SYMVRELLIQ
660 670 680 690 700
FYKSTRFKPT RIIFYRDGVP EGQLPQILHY ELLAIRDACI KLEKDYQPGI
710 720 730 740 750
TYIVVQKRHH TRLFCADKNE RIGKSGNIPA GTTVDTNITH PFEFDFYLCS
760 770 780 790 800
HAGIQGTSRP SHYYVLWDDN RFTADELQIL TYQLCHTYVR CTRSVSIPAP
810 820 830 840 850
AYYARLVAFR ARYHLVDKEH DSGEGSHISG QSNGRDPQAL AKAVQVHQDT

LRTMYFA
Length:857
Mass (Da):97,214
Last modified:July 27, 2011 - v2
Checksum:i1DBB524AE7CBAF66
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96K → E in BAC15766 (PubMed:12526743).Curated1
Sequence conflicti309Y → H in BAC15766 (PubMed:12526743).Curated1
Sequence conflicti386Y → C in BAC15766 (PubMed:12526743).Curated1
Sequence conflicti550V → A in BAC15766 (PubMed:12526743).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081471 mRNA Translation: BAC15766.1
AL606935, AL606976 Genomic DNA Translation: CAM17423.1
AL606976, AL606935 Genomic DNA Translation: CAM45874.1
BC129914 mRNA Translation: AAI29915.1
CCDSiCCDS18653.1
RefSeqiNP_001304102.1, NM_001317173.1
NP_700452.2, NM_153403.3
UniGeneiMm.30800

Genome annotation databases

EnsembliENSMUST00000097888; ENSMUSP00000095498; ENSMUSG00000041530
GeneIDi236511
KEGGimmu:236511
UCSCiuc008utj.2 mouse

Similar proteinsi

Entry informationi

Entry nameiAGO1_MOUSE
AccessioniPrimary (citable) accession number: Q8CJG1
Secondary accession number(s): A1L365
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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