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Q8CJG1

- AGO1_MOUSE

UniProt

Q8CJG1 - AGO1_MOUSE

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Protein

Protein argonaute-1

Gene
Ago1, Eif2c1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. May also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. RNA binding Source: MGI

GO - Biological processi

  1. negative regulation of translation involved in gene silencing by miRNA Source: UniProtKB
  2. nuclear-transcribed mRNA catabolic process Source: UniProtKB
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor, Ribonucleoprotein

Keywords - Biological processi

RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiREACT_198208. Post-transcriptional silencing by small RNAs.
REACT_201975. Small interfering RNA (siRNA) biogenesis.
REACT_221178. MicroRNA (miRNA) biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute-1
Short name:
Argonaute1
Short name:
mAgo1
Alternative name(s):
Argonaute RISC catalytic component 1
Eukaryotic translation initiation factor 2C 1
Short name:
eIF-2C 1
Short name:
eIF2C 1
Piwi/argonaute family protein meIF2C1
Gene namesi
Name:Ago1
Synonyms:Eif2c1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:2446630. Ago1.

Subcellular locationi

CytoplasmP-body By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytoplasmic mRNA processing body Source: MGI
  3. micro-ribonucleoprotein complex Source: UniProtKB
  4. polysome Source: UniProtKB
  5. ribonucleoprotein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 857857Protein argonaute-1PRO_0000194056Add
BLAST

Proteomic databases

PaxDbiQ8CJG1.
PRIDEiQ8CJG1.

PTM databases

PhosphoSiteiQ8CJG1.

Expressioni

Gene expression databases

ArrayExpressiQ8CJG1.
BgeeiQ8CJG1.
CleanExiMM_EIF2C1.
GenevestigatoriQ8CJG1.

Interactioni

Subunit structurei

Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs) By similarity. Interacts with LIMD1, WTIP and AJUBA By similarity. Interacts with APOBEC3F, APOBEC3G and APOBEC3H By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Trim32Q8CH722EBI-2291996,EBI-773837

Protein-protein interaction databases

BioGridi231767. 1 interaction.
IntActiQ8CJG1. 2 interactions.
MINTiMINT-4098243.
STRINGi10090.ENSMUSP00000095498.

Structurei

3D structure databases

ProteinModelPortaliQ8CJG1.
SMRiQ8CJG1. Positions 17-857.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini226 – 346121PAZAdd
BLAST
Domaini515 – 816302PiwiAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni309 – 3146Interaction with guide RNA By similarity
Regioni522 – 56443Interaction with guide RNA By similarityAdd
BLAST
Regioni670 – 6756Impairs access of bound RNA to the active site By similarity
Regioni708 – 7125Interaction with guide RNA By similarity
Regioni751 – 7599Interaction with guide RNA By similarity
Regioni788 – 81326Interaction with guide RNA By similarityAdd
BLAST

Sequence similaritiesi

Contains 1 PAZ domain.
Contains 1 Piwi domain.

Phylogenomic databases

eggNOGiNOG279895.
GeneTreeiENSGT00640000091310.
HOGENOMiHOG000116043.
HOVERGENiHBG006101.
InParanoidiA1L365.
KOiK11593.
OMAiEVTHRAN.
OrthoDBiEOG7HHWRC.
TreeFamiTF101510.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR014811. DUF1785.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. DUF1785. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CJG1-1 [UniParc]FASTAAdd to Basket

« Hide

MEAGPSGAAA GAYLPPLQQV FQAPRRPGIG TVGKPIKLLA NYFEVDIPKI    50
DVYHYEVDIK PDKCPRRVNR EVVEYMVQHF KPQIFGDRKP VYDGKKNIYT 100
VTALPIGNER VDFEVTIPGE GKDRIFKVSI KWLAIVSWRM LHEALVSGQI 150
PVPLESVQAL DVAMRHLASM RYTPVGRSFF SPPEGYYHPL GGGREVWFGF 200
HQSVRPAMWK MMLNIDVSAT AFYKAQPVIE FMCEVLDIRN IDEQPKPLTD 250
SQRVRFTKEI KGLKVEVTHC GQMKRKYRVC NVTRRPASHQ TFPLQLESGQ 300
TVECTVAQYF KQKYNLQLKY PHLPCLQVGQ EQKHTYLPLE VCNIVAGQRC 350
IKKLTDNQTS TMIKATARSA PDRQEEISRL MKNASYNLDP YIQEFGIKVK 400
DDMTEVTGRV LPAPILQYGG RNRAIATPNQ GVWDMRGKQF YNGIEIKVWA 450
IACFAPQKQC REEVLKNFTD QLRKISKDAG MPIQGQPCFC KYAQGADSVE 500
PMFRHLKNTY SGLQLIIVIL PGKTPVYAEV KRVGDTLLGM ATQCVQVKNV 550
VKTSPQTLSN LCLKINVKLG GINNILVPHQ RSAVFQQPVI FLGADVTHPP 600
AGDGKKPSIT AVVGSMDAHP SRYCATVRVQ RPRQEIIEDL SYMVRELLIQ 650
FYKSTRFKPT RIIFYRDGVP EGQLPQILHY ELLAIRDACI KLEKDYQPGI 700
TYIVVQKRHH TRLFCADKNE RIGKSGNIPA GTTVDTNITH PFEFDFYLCS 750
HAGIQGTSRP SHYYVLWDDN RFTADELQIL TYQLCHTYVR CTRSVSIPAP 800
AYYARLVAFR ARYHLVDKEH DSGEGSHISG QSNGRDPQAL AKAVQVHQDT 850
LRTMYFA 857
Length:857
Mass (Da):97,214
Last modified:July 27, 2011 - v2
Checksum:i1DBB524AE7CBAF66
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961K → E in BAC15766. 1 Publication
Sequence conflicti309 – 3091Y → H in BAC15766. 1 Publication
Sequence conflicti386 – 3861Y → C in BAC15766. 1 Publication
Sequence conflicti550 – 5501V → A in BAC15766. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB081471 mRNA. Translation: BAC15766.1.
AL606935, AL606976 Genomic DNA. Translation: CAM17423.1.
AL606976, AL606935 Genomic DNA. Translation: CAM45874.1.
BC129914 mRNA. Translation: AAI29915.1.
CCDSiCCDS18653.1.
RefSeqiNP_700452.2. NM_153403.2.
UniGeneiMm.30800.

Genome annotation databases

EnsembliENSMUST00000097888; ENSMUSP00000095498; ENSMUSG00000041530.
GeneIDi236511.
KEGGimmu:236511.
UCSCiuc008utj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB081471 mRNA. Translation: BAC15766.1 .
AL606935 , AL606976 Genomic DNA. Translation: CAM17423.1 .
AL606976 , AL606935 Genomic DNA. Translation: CAM45874.1 .
BC129914 mRNA. Translation: AAI29915.1 .
CCDSi CCDS18653.1.
RefSeqi NP_700452.2. NM_153403.2.
UniGenei Mm.30800.

3D structure databases

ProteinModelPortali Q8CJG1.
SMRi Q8CJG1. Positions 17-857.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 231767. 1 interaction.
IntActi Q8CJG1. 2 interactions.
MINTi MINT-4098243.
STRINGi 10090.ENSMUSP00000095498.

PTM databases

PhosphoSitei Q8CJG1.

Proteomic databases

PaxDbi Q8CJG1.
PRIDEi Q8CJG1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000097888 ; ENSMUSP00000095498 ; ENSMUSG00000041530 .
GeneIDi 236511.
KEGGi mmu:236511.
UCSCi uc008utj.2. mouse.

Organism-specific databases

CTDi 26523.
MGIi MGI:2446630. Ago1.

Phylogenomic databases

eggNOGi NOG279895.
GeneTreei ENSGT00640000091310.
HOGENOMi HOG000116043.
HOVERGENi HBG006101.
InParanoidi A1L365.
KOi K11593.
OMAi EVTHRAN.
OrthoDBi EOG7HHWRC.
TreeFami TF101510.

Enzyme and pathway databases

Reactomei REACT_198208. Post-transcriptional silencing by small RNAs.
REACT_201975. Small interfering RNA (siRNA) biogenesis.
REACT_221178. MicroRNA (miRNA) biogenesis.

Miscellaneous databases

NextBioi 382987.
PROi Q8CJG1.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8CJG1.
Bgeei Q8CJG1.
CleanExi MM_EIF2C1.
Genevestigatori Q8CJG1.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
InterProi IPR014811. DUF1785.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF08699. DUF1785. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view ]
SMARTi SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view ]
SUPFAMi SSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEi PS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors."
    Doi N., Zenno S., Ueda R., Ohki-Hamazaki H., Ui-Tei K., Saigo K.
    Curr. Biol. 13:41-46(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Essential and overlapping functions for mammalian Argonautes in microRNA silencing."
    Su H., Trombly M.I., Chen J., Wang X.
    Genes Dev. 23:304-317(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAGO1_MOUSE
AccessioniPrimary (citable) accession number: Q8CJG1
Secondary accession number(s): A1L365
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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