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Protein

Protein argonaute-3

Gene

Ago3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processRNA-mediated gene silencing, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-203927 MicroRNA (miRNA) biogenesis
R-MMU-426486 Small interfering RNA (siRNA) biogenesis
R-MMU-426496 Post-transcriptional silencing by small RNAs

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute-3UniRule annotation
Short name:
Argonaute3UniRule annotation
Short name:
mAgo3
Alternative name(s):
Argonaute RISC catalytic component 3
Eukaryotic translation initiation factor 2C 3UniRule annotation
Short name:
eIF-2C 3UniRule annotation
Short name:
eIF2C 3UniRule annotation
Piwi/argonaute family protein meIF2C3
Gene namesi
Name:Ago3
Synonyms:Eif2c3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:2446634 Ago3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940621 – 860Protein argonaute-3Add BLAST860

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei825PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8CJF9
MaxQBiQ8CJF9
PaxDbiQ8CJF9
PeptideAtlasiQ8CJF9
PRIDEiQ8CJF9

PTM databases

iPTMnetiQ8CJF9
PhosphoSitePlusiQ8CJF9

Expressioni

Gene expression databases

BgeeiENSMUSG00000028842
CleanExiMM_EIF2C3
ExpressionAtlasiQ8CJF9 baseline and differential
GenevisibleiQ8CJF9 MM

Interactioni

Subunit structurei

Interacts with EIF4B, IMP8, PRMT5 and TNRC6B (By similarity). Interacts with APOBEC3F, APOBEC3G and APOBEC3H. Interacts with EDC4 (By similarity).UniRule annotation

Protein-protein interaction databases

BioGridi229499, 1 interactor
IntActiQ8CJF9, 2 interactors
STRINGi10090.ENSMUSP00000066633

Structurei

3D structure databases

ProteinModelPortaliQ8CJF9
SMRiQ8CJF9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini236 – 349PAZUniRule annotationAdd BLAST114
Domaini518 – 819PiwiUniRule annotationAdd BLAST302

Sequence similaritiesi

Belongs to the argonaute family. Ago subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG1041 Eukaryota
ENOG410XP07 LUCA
GeneTreeiENSGT00760000119148
HOGENOMiHOG000116043
HOVERGENiHBG006101
InParanoidiQ8CJF9
KOiK11593
OMAiQPANPQY
OrthoDBiEOG091G020J
TreeFamiTF101510

Family and domain databases

Gene3Di3.30.420.10, 1 hit
HAMAPiMF_03032 AGO3, 1 hit
InterProiView protein in InterPro
IPR028603 AGO3
IPR014811 ArgoL1
IPR032472 ArgoL2
IPR032473 Argonaute_Mid_dom
IPR032474 Argonaute_N
IPR003100 PAZ_dom
IPR036085 PAZ_dom_sf
IPR003165 Piwi
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF08699 ArgoL1, 1 hit
PF16488 ArgoL2, 1 hit
PF16487 ArgoMid, 1 hit
PF16486 ArgoN, 1 hit
PF02170 PAZ, 1 hit
PF02171 Piwi, 1 hit
SMARTiView protein in SMART
SM01163 DUF1785, 1 hit
SM00949 PAZ, 1 hit
SM00950 Piwi, 1 hit
SUPFAMiSSF101690 SSF101690, 1 hit
SSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS50821 PAZ, 1 hit
PS50822 PIWI, 1 hit

Sequencei

Sequence statusi: Complete.

Q8CJF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIGSAGPIG AQPLFIVPRR PGYGTMGKPI KLLANCFQVE IPKIDVYLYE
60 70 80 90 100
VDIKPDKCPR RVNREVVDSM VQHFKVTIFG DRRPVYDGKR SLYTANPLPV
110 120 130 140 150
ATTGVDLDVT LPGEGGKDRP FKVSVKFVSR VSWHLLHEAL AGGTLPEPLE
160 170 180 190 200
LDKPVSTNPV HAVDVVLRHL PSMKYTPVGR SFFSAPEGYD HPLGGGREVW
210 220 230 240 250
FGFHQSVRPA MWKMMLNIDV SATAFYKAQP VIQFMCEVLD IHNIDEQPRP
260 270 280 290 300
LTDSHRVKFT KEIKGLKVEV THCGTMRRKY RVCNVTRRPA SHQTFPLQLE
310 320 330 340 350
NGQTVERTVA QYFREKYTLQ LKYPHLPCLQ VGQEQKHTYL PLEVCNIVAG
360 370 380 390 400
QRCIKKLTDN QTSTMIKATA RSAPDRQEEI SRLVRSANYE TDPFVQEFQF
410 420 430 440 450
KVRDEMAHVT GRVLPAPMLQ YGGRNRTVAT PSHGVWDMRG KQFHTGVEIK
460 470 480 490 500
MWAIACFATQ RQCREEILKG FTDQLRKISK DAGMPIQGQP CFCKYAQGAD
510 520 530 540 550
SVEPMFRHLK NTYSGLQLII VILPGKTPVY AEVKRVGDTL LGMATQCVQV
560 570 580 590 600
KNVIKTSPQT LSNLCLKINV KLGGINNILV PHQRPSVFQQ PVIFLGADVT
610 620 630 640 650
HPPAGDGKKP SIAAVVGSMD AHPSRYCATV RVQRPRQEII QDLASMVREL
660 670 680 690 700
LIQFYKSTRF KPTRIIFYRD GVSEGQFRQV LYYELLAIRE ACISLEKDYQ
710 720 730 740 750
PGITYIVVQK RHHTRLFCAD RTERVGRSGN IPAGTTVDTD ITHPYEFDFY
760 770 780 790 800
LCSHAGIQGT SRPSHYHVLW DDNFFTADEL QLLTYQLCHT YVRCTRSVSI
810 820 830 840 850
PAPAYYAHLV AFRARYHLVD KEHDSAEGSH VSGQSNGRDP QALAKAVQIH
860
QDTLRTMYFA
Length:860
Mass (Da):97,277
Last modified:July 27, 2011 - v2
Checksum:iB457A31093BB5BB0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70M → K in BAC15768 (PubMed:12526743).Curated1
Sequence conflicti400F → L in BAC15768 (PubMed:12526743).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081473 mRNA Translation: BAC15768.1
AK154436 mRNA Translation: BAE32586.1
AK171118 mRNA Translation: BAE42260.1
AL606976 Genomic DNA Translation: CAM45883.1
CH466552 Genomic DNA Translation: EDL30287.1
BC137964 mRNA Translation: AAI37965.1
CCDSiCCDS18652.1
RefSeqiNP_700451.2, NM_153402.2
UniGeneiMm.440915
Mm.482388
Mm.483093

Genome annotation databases

EnsembliENSMUST00000069097; ENSMUSP00000066633; ENSMUSG00000028842
GeneIDi214150
KEGGimmu:214150
UCSCiuc008uth.2 mouse

Entry informationi

Entry nameiAGO3_MOUSE
AccessioniPrimary (citable) accession number: Q8CJF9
Secondary accession number(s): Q3TBP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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