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Protein

Protein argonaute-3

Gene

Ago3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

RNA-mediated gene silencing, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-426496. Post-transcriptional silencing by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute-3UniRule annotation
Short name:
Argonaute3UniRule annotation
Short name:
mAgo3
Alternative name(s):
Argonaute RISC catalytic component 3
Eukaryotic translation initiation factor 2C 3UniRule annotation
Short name:
eIF-2C 3UniRule annotation
Short name:
eIF2C 3UniRule annotation
Piwi/argonaute family protein meIF2C3
Gene namesi
Name:Ago3
Synonyms:Eif2c3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2446634. Ago3.

Subcellular locationi

  • CytoplasmP-body UniRule annotation

GO - Cellular componenti

  • condensed nuclear chromosome Source: MGI
  • cytoplasm Source: MGI
  • cytoplasmic mRNA processing body Source: UniProtKB-SubCell
  • membrane Source: MGI
  • micro-ribonucleoprotein complex Source: MGI
  • RISC complex Source: MGI
  • RISC-loading complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 860860Protein argonaute-3PRO_0000194062Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8CJF9.
MaxQBiQ8CJF9.
PaxDbiQ8CJF9.
PRIDEiQ8CJF9.

PTM databases

iPTMnetiQ8CJF9.
PhosphoSiteiQ8CJF9.

Expressioni

Gene expression databases

BgeeiQ8CJF9.
CleanExiMM_EIF2C3.
ExpressionAtlasiQ8CJF9. baseline and differential.
GenevisibleiQ8CJF9. MM.

Interactioni

Subunit structurei

Interacts with EIF4B, IMP8, PRMT5 and TNRC6B (By similarity). Interacts with APOBEC3F, APOBEC3G and APOBEC3H. Interacts with EDC4 (By similarity).UniRule annotation

Protein-protein interaction databases

IntActiQ8CJF9. 1 interaction.
STRINGi10090.ENSMUSP00000066633.

Structurei

3D structure databases

ProteinModelPortaliQ8CJF9.
SMRiQ8CJF9. Positions 12-860.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini236 – 349114PAZUniRule annotationAdd
BLAST
Domaini518 – 819302PiwiUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the argonaute family. Ago subfamily.UniRule annotation
Contains 1 PAZ domain.UniRule annotation
Contains 1 Piwi domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG1041. Eukaryota.
ENOG410XP07. LUCA.
GeneTreeiENSGT00760000119148.
HOGENOMiHOG000116043.
HOVERGENiHBG006101.
InParanoidiQ8CJF9.
KOiK11593.
OMAiKSLYTAN.
OrthoDBiEOG7HHWRC.
TreeFamiTF101510.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_03032. AGO3.
InterProiIPR028603. AGO3.
IPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032473. Argonaute_Mid_dom.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16487. ArgoMid. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM01163. DUF1785. 1 hit.
SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CJF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIGSAGPIG AQPLFIVPRR PGYGTMGKPI KLLANCFQVE IPKIDVYLYE
60 70 80 90 100
VDIKPDKCPR RVNREVVDSM VQHFKVTIFG DRRPVYDGKR SLYTANPLPV
110 120 130 140 150
ATTGVDLDVT LPGEGGKDRP FKVSVKFVSR VSWHLLHEAL AGGTLPEPLE
160 170 180 190 200
LDKPVSTNPV HAVDVVLRHL PSMKYTPVGR SFFSAPEGYD HPLGGGREVW
210 220 230 240 250
FGFHQSVRPA MWKMMLNIDV SATAFYKAQP VIQFMCEVLD IHNIDEQPRP
260 270 280 290 300
LTDSHRVKFT KEIKGLKVEV THCGTMRRKY RVCNVTRRPA SHQTFPLQLE
310 320 330 340 350
NGQTVERTVA QYFREKYTLQ LKYPHLPCLQ VGQEQKHTYL PLEVCNIVAG
360 370 380 390 400
QRCIKKLTDN QTSTMIKATA RSAPDRQEEI SRLVRSANYE TDPFVQEFQF
410 420 430 440 450
KVRDEMAHVT GRVLPAPMLQ YGGRNRTVAT PSHGVWDMRG KQFHTGVEIK
460 470 480 490 500
MWAIACFATQ RQCREEILKG FTDQLRKISK DAGMPIQGQP CFCKYAQGAD
510 520 530 540 550
SVEPMFRHLK NTYSGLQLII VILPGKTPVY AEVKRVGDTL LGMATQCVQV
560 570 580 590 600
KNVIKTSPQT LSNLCLKINV KLGGINNILV PHQRPSVFQQ PVIFLGADVT
610 620 630 640 650
HPPAGDGKKP SIAAVVGSMD AHPSRYCATV RVQRPRQEII QDLASMVREL
660 670 680 690 700
LIQFYKSTRF KPTRIIFYRD GVSEGQFRQV LYYELLAIRE ACISLEKDYQ
710 720 730 740 750
PGITYIVVQK RHHTRLFCAD RTERVGRSGN IPAGTTVDTD ITHPYEFDFY
760 770 780 790 800
LCSHAGIQGT SRPSHYHVLW DDNFFTADEL QLLTYQLCHT YVRCTRSVSI
810 820 830 840 850
PAPAYYAHLV AFRARYHLVD KEHDSAEGSH VSGQSNGRDP QALAKAVQIH
860
QDTLRTMYFA
Length:860
Mass (Da):97,277
Last modified:July 27, 2011 - v2
Checksum:iB457A31093BB5BB0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701M → K in BAC15768 (PubMed:12526743).Curated
Sequence conflicti400 – 4001F → L in BAC15768 (PubMed:12526743).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081473 mRNA. Translation: BAC15768.1.
AK154436 mRNA. Translation: BAE32586.1.
AK171118 mRNA. Translation: BAE42260.1.
AL606976 Genomic DNA. Translation: CAM45883.1.
CH466552 Genomic DNA. Translation: EDL30287.1.
BC137964 mRNA. Translation: AAI37965.1.
CCDSiCCDS18652.1.
RefSeqiNP_700451.2. NM_153402.2.
UniGeneiMm.440915.
Mm.482388.
Mm.483093.

Genome annotation databases

EnsembliENSMUST00000069097; ENSMUSP00000066633; ENSMUSG00000028842.
GeneIDi214150.
KEGGimmu:214150.
UCSCiuc008uth.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081473 mRNA. Translation: BAC15768.1.
AK154436 mRNA. Translation: BAE32586.1.
AK171118 mRNA. Translation: BAE42260.1.
AL606976 Genomic DNA. Translation: CAM45883.1.
CH466552 Genomic DNA. Translation: EDL30287.1.
BC137964 mRNA. Translation: AAI37965.1.
CCDSiCCDS18652.1.
RefSeqiNP_700451.2. NM_153402.2.
UniGeneiMm.440915.
Mm.482388.
Mm.483093.

3D structure databases

ProteinModelPortaliQ8CJF9.
SMRiQ8CJF9. Positions 12-860.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CJF9. 1 interaction.
STRINGi10090.ENSMUSP00000066633.

PTM databases

iPTMnetiQ8CJF9.
PhosphoSiteiQ8CJF9.

Proteomic databases

EPDiQ8CJF9.
MaxQBiQ8CJF9.
PaxDbiQ8CJF9.
PRIDEiQ8CJF9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069097; ENSMUSP00000066633; ENSMUSG00000028842.
GeneIDi214150.
KEGGimmu:214150.
UCSCiuc008uth.2. mouse.

Organism-specific databases

CTDi192669.
MGIiMGI:2446634. Ago3.

Phylogenomic databases

eggNOGiKOG1041. Eukaryota.
ENOG410XP07. LUCA.
GeneTreeiENSGT00760000119148.
HOGENOMiHOG000116043.
HOVERGENiHBG006101.
InParanoidiQ8CJF9.
KOiK11593.
OMAiKSLYTAN.
OrthoDBiEOG7HHWRC.
TreeFamiTF101510.

Enzyme and pathway databases

ReactomeiR-MMU-426496. Post-transcriptional silencing by small RNAs.

Miscellaneous databases

PROiQ8CJF9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CJF9.
CleanExiMM_EIF2C3.
ExpressionAtlasiQ8CJF9. baseline and differential.
GenevisibleiQ8CJF9. MM.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_03032. AGO3.
InterProiIPR028603. AGO3.
IPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032473. Argonaute_Mid_dom.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16487. ArgoMid. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM01163. DUF1785. 1 hit.
SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors."
    Doi N., Zenno S., Ueda R., Ohki-Hamazaki H., Ui-Tei K., Saigo K.
    Curr. Biol. 13:41-46(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "Essential and overlapping functions for mammalian Argonautes in microRNA silencing."
    Su H., Trombly M.I., Chen J., Wang X.
    Genes Dev. 23:304-317(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAGO3_MOUSE
AccessioniPrimary (citable) accession number: Q8CJF9
Secondary accession number(s): Q3TBP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.