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Protein

Protein argonaute-4

Gene

Ago4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

  • male gonad development Source: MGI
  • male meiosis Source: MGI
  • miRNA loading onto RISC involved in gene silencing by miRNA Source: MGI
  • miRNA mediated inhibition of translation Source: UniProtKB
  • miRNA metabolic process Source: MGI
  • mRNA catabolic process Source: UniProtKB
  • negative regulation of apoptotic process Source: MGI
  • pre-miRNA processing Source: MGI
  • production of miRNAs involved in gene silencing by miRNA Source: MGI
  • regulation of cell morphogenesis Source: MGI
  • RNA secondary structure unwinding Source: MGI
  • synaptonemal complex assembly Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

RNA-mediated gene silencing, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-426496. Post-transcriptional silencing by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute-4UniRule annotation
Short name:
Argonaute4UniRule annotation
Short name:
mAgo4
Alternative name(s):
Argonaute RISC catalytic component 4
Eukaryotic translation initiation factor 2C 4UniRule annotation
Short name:
eIF-2C 4UniRule annotation
Short name:
eIF2C 4UniRule annotation
Piwi/argonaute family protein meIF2C4
Gene namesi
Name:Ago4
Synonyms:Eif2c4, Kiaa1567
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1924100. Ago4.

Subcellular locationi

  • CytoplasmP-body UniRule annotation

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoplasmic mRNA processing body Source: MGI
  • membrane Source: MGI
  • micro-ribonucleoprotein complex Source: MGI
  • nucleus Source: MGI
  • RISC complex Source: MGI
  • RISC-loading complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 861861Protein argonaute-4PRO_0000194064Add
BLAST

Proteomic databases

EPDiQ8CJF8.
MaxQBiQ8CJF8.
PaxDbiQ8CJF8.
PRIDEiQ8CJF8.

PTM databases

iPTMnetiQ8CJF8.
PhosphoSiteiQ8CJF8.

Expressioni

Gene expression databases

BgeeiQ8CJF8.
GenevisibleiQ8CJF8. MM.

Interactioni

Subunit structurei

Interacts with EIF4B, IMP8, PRMT5, TNRC6A and TNRC6B.UniRule annotation

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000081312.

Structurei

3D structure databases

ProteinModelPortaliQ8CJF8.
SMRiQ8CJF8. Positions 12-861.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini225 – 338114PAZUniRule annotationAdd
BLAST
Domaini509 – 820312PiwiUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the argonaute family. Ago subfamily.UniRule annotation
Contains 1 PAZ domain.UniRule annotation
Contains 1 Piwi domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG1041. Eukaryota.
ENOG410XP07. LUCA.
GeneTreeiENSGT00760000119148.
HOGENOMiHOG000116043.
InParanoidiQ8CJF8.
KOiK11593.
OMAiADKTERX.
OrthoDBiEOG7HHWRC.
PhylomeDBiQ8CJF8.
TreeFamiTF101510.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_03033. AGO4.
InterProiIPR028604. AGO4.
IPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032473. Argonaute_Mid_dom.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16487. ArgoMid. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM01163. DUF1785. 1 hit.
SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CJF8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEALGPGPPA SLFQPPRRPG LGTVGKPIRL LANHFQVQIP KIDVYHYDVD
60 70 80 90 100
IKPEKRPRRV NREVVDTMVR HFKMQIFGDR QPGYDGKRNM YTAHPLPIGR
110 120 130 140 150
DRIDMEVTLP GEGKDQTFKV SVQWVSVVSL QLLLEALAGH LNEVPDDSVQ
160 170 180 190 200
ALDVITRHLP SMRYTPVGRS FFSPPEGYYH PLGGGREVWF GFHQSVRPAM
210 220 230 240 250
WNMMLNIDVS ATAFYRAQPI IEFMCEVLDI QNINEQTKPL TDSQRVKFTK
260 270 280 290 300
EIRGLKVEVT HCGQMKRKYR VCNVTRRPAS HQTFPLQLEN GQAMECTVAQ
310 320 330 340 350
YFKQKYSLQL KHPHLPCLQV GQEQKHTYLP LEVCNIVAGQ RCIKKLTDNQ
360 370 380 390 400
TSTMIKATAR SAPDRQEEIS RLVKSNSMVG GPDPYLKEFG IVVHNEMTEL
410 420 430 440 450
TGRVLPAPML QYGGRNKTVA TPSQGVWDMR GKQFYAGIEI KVWAVACFAP
460 470 480 490 500
QKQCREDLLK SFTDQLRKIS KDAGMPIQGQ PCFCKYAQGA DSVEPMFKHL
510 520 530 540 550
KMTYVGLQLI VVILPGKTPV YAEVKRVGDT LLGMATQCVQ VKNVVKTSPQ
560 570 580 590 600
TLSNLCLKMN AKLGGINNVL VPHQRPSVFQ QPVIFLGADV THPPAGDGKK
610 620 630 640 650
PSIAAVVGSM DGHPSRYCAT VRVQTSRQEI TQELLYSQEV VQDLTSMARE
660 670 680 690 700
LLIQFYKSTR FKPTRIIYYR GGVSEGQMKQ VAWPELIAIR KACISLEEDY
710 720 730 740 750
RPGITYIVVQ KRHHTRLFCA DKMERVGKSG NVPAGTTVDS TVTHPSEFDF
760 770 780 790 800
YLCSHAGIQG TSRPSHYQVL WDDNCFTADE LQLLTYQLCH TYVRCTRSVS
810 820 830 840 850
IPAPAYYARL VAFRARYHLV DKDHDSAEGS HVSGQSNGRD PQALAKAVQI
860
HHDTQHTMYF A
Length:861
Mass (Da):97,037
Last modified:March 3, 2009 - v2
Checksum:i5C570D5A22414E14
GO
Isoform 2 (identifier: Q8CJF8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     399-460: Missing.

Note: No experimental confirmation available.
Show »
Length:799
Mass (Da):90,102
Checksum:iF3FBC51B27BA43D8
GO

Sequence cautioni

The sequence BAC26738.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC98205.2 differs from that shown.Intron retention.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti128 – 1281V → A in BAC15769 (PubMed:12526743).Curated
Sequence conflicti541 – 5411V → I in BAC15769 (PubMed:12526743).Curated
Sequence conflicti570 – 5701L → P in BAC15769 (PubMed:12526743).Curated
Sequence conflicti622 – 6221R → W in BAC15769 (PubMed:12526743).Curated
Sequence conflicti631 – 6311T → A in BAC15769 (PubMed:12526743).Curated
Sequence conflicti649 – 6491R → W in BAC98205 (PubMed:14621295).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei399 – 46062Missing in isoform 2. 1 PublicationVSP_036487Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081474 mRNA. Translation: BAC15769.1.
AK030018 mRNA. Translation: BAC26738.1. Different initiation.
AL606935 Genomic DNA. Translation: CAM17428.1.
BC096023 mRNA. Translation: AAH96023.1.
AK129395 Transcribed RNA. Translation: BAC98205.2. Sequence problems.
CCDSiCCDS18654.1. [Q8CJF8-1]
RefSeqiNP_694817.2. NM_153177.3. [Q8CJF8-1]
UniGeneiMm.86705.

Genome annotation databases

EnsembliENSMUST00000084289; ENSMUSP00000081312; ENSMUSG00000042500. [Q8CJF8-1]
GeneIDi76850.
KEGGimmu:76850.
UCSCiuc008utk.2. mouse. [Q8CJF8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081474 mRNA. Translation: BAC15769.1.
AK030018 mRNA. Translation: BAC26738.1. Different initiation.
AL606935 Genomic DNA. Translation: CAM17428.1.
BC096023 mRNA. Translation: AAH96023.1.
AK129395 Transcribed RNA. Translation: BAC98205.2. Sequence problems.
CCDSiCCDS18654.1. [Q8CJF8-1]
RefSeqiNP_694817.2. NM_153177.3. [Q8CJF8-1]
UniGeneiMm.86705.

3D structure databases

ProteinModelPortaliQ8CJF8.
SMRiQ8CJF8. Positions 12-861.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000081312.

PTM databases

iPTMnetiQ8CJF8.
PhosphoSiteiQ8CJF8.

Proteomic databases

EPDiQ8CJF8.
MaxQBiQ8CJF8.
PaxDbiQ8CJF8.
PRIDEiQ8CJF8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084289; ENSMUSP00000081312; ENSMUSG00000042500. [Q8CJF8-1]
GeneIDi76850.
KEGGimmu:76850.
UCSCiuc008utk.2. mouse. [Q8CJF8-1]

Organism-specific databases

CTDi192670.
MGIiMGI:1924100. Ago4.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1041. Eukaryota.
ENOG410XP07. LUCA.
GeneTreeiENSGT00760000119148.
HOGENOMiHOG000116043.
InParanoidiQ8CJF8.
KOiK11593.
OMAiADKTERX.
OrthoDBiEOG7HHWRC.
PhylomeDBiQ8CJF8.
TreeFamiTF101510.

Enzyme and pathway databases

ReactomeiR-MMU-426496. Post-transcriptional silencing by small RNAs.

Miscellaneous databases

PROiQ8CJF8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CJF8.
GenevisibleiQ8CJF8. MM.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_03033. AGO4.
InterProiIPR028604. AGO4.
IPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032473. Argonaute_Mid_dom.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16487. ArgoMid. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM01163. DUF1785. 1 hit.
SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors."
    Doi N., Zenno S., Ueda R., Ohki-Hamazaki H., Ui-Tei K., Saigo K.
    Curr. Biol. 13:41-46(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Eye.
  5. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 144-861 (ISOFORM 2).
    Tissue: Pancreatic islet.
  6. "Essential and overlapping functions for mammalian Argonautes in microRNA silencing."
    Su H., Trombly M.I., Chen J., Wang X.
    Genes Dev. 23:304-317(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAGO4_MOUSE
AccessioniPrimary (citable) accession number: Q8CJF8
Secondary accession number(s): Q4VBD7, Q6ZPM6, Q8BTF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: March 3, 2009
Last modified: June 8, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.