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Protein

E3 ubiquitin-protein ligase BRE1B

Gene

Rnf40

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri949 – 98840RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • mRNA 3'-UTR binding Source: Ensembl
  • protein complex binding Source: RGD
  • syntaxin-1 binding Source: RGD
  • ubiquitin conjugating enzyme binding Source: RGD
  • ubiquitin-protein transferase activity Source: RGD
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.3.2.B10. 5301.
6.3.2.19. 5301.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase BRE1B (EC:6.3.2.-)
Short name:
BRE1-B
Alternative name(s):
RING finger protein 40
Syntaxin-1-interacting RING finger protein
Short name:
Protein staring
Gene namesi
Name:Rnf40
Synonyms:Bre1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi628638. Rnf40.

Subcellular locationi

GO - Cellular componenti

  • axon terminus Source: RGD
  • cytosol Source: RGD
  • extrinsic component of membrane Source: RGD
  • HULC complex Source: UniProtKB
  • neuron projection Source: RGD
  • nucleoplasm Source: Ensembl
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10021002E3 ubiquitin-protein ligase BRE1BPRO_0000055843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201N6-acetyllysineBy similarity
Modified residuei356 – 3561N6-acetyllysineBy similarity
Modified residuei518 – 5181N6-acetyllysineBy similarity
Modified residuei585 – 5851PhosphoserineCombined sources
Modified residuei586 – 5861PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8CJB9.
PRIDEiQ8CJB9.

PTM databases

iPTMnetiQ8CJB9.
PhosphoSiteiQ8CJB9.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed in brain, testis, heart, liver and kidney. Weakly expressed in lung, spleen and skeletal muscle (at protein level).1 Publication

Gene expression databases

GenevisibleiQ8CJB9. RN.

Interactioni

Subunit structurei

Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with RB1 and WAC (By similarity). May interact with STX1A.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Stx1bP612654EBI-6110162,EBI-2255905
UBE2L6O149332EBI-6110162,EBI-2129974From a different organism.

GO - Molecular functioni

  • protein complex binding Source: RGD
  • syntaxin-1 binding Source: RGD
  • ubiquitin conjugating enzyme binding Source: RGD

Protein-protein interaction databases

IntActiQ8CJB9. 2 interactions.
STRINGi10116.ENSRNOP00000025500.

Structurei

3D structure databases

ProteinModelPortaliQ8CJB9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili55 – 9137Sequence analysisAdd
BLAST
Coiled coili190 – 378189Sequence analysisAdd
BLAST
Coiled coili438 – 52689Sequence analysisAdd
BLAST
Coiled coili628 – 947320Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the BRE1 family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri949 – 98840RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0978. Eukaryota.
ENOG410Y5C6. LUCA.
GeneTreeiENSGT00390000002866.
HOGENOMiHOG000231526.
HOVERGENiHBG080312.
InParanoidiQ8CJB9.
KOiK10696.
OMAiPNLGHPE.
OrthoDBiEOG7DVD99.
PhylomeDBiQ8CJB9.
TreeFamiTF323183.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CJB9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLSNKRAA GDGGSGPPEK KLNREEKTTT TLIEPIRLGG ISSTEEMDSK
60 70 80 90 100
VLQFKNKKLA ERLEQRQACE DELRERIEKL EKRQATDDAT LLIVNRYWAQ
110 120 130 140 150
LDETVEALLQ CYENQRELSS SGTEVPGCQE GLTRDVIPRT DPGTSDLREP
160 170 180 190 200
LPMQFRAPLS EPALAFVVAL GASSCEEVEL QLQGRMEFSK AAVSRVVEAS
210 220 230 240 250
DRLQRQVEEL CQRVYSRGDS EAPGEVARAR TRELGRENRR LQDLATQLQE
260 270 280 290 300
KHHRISLEYS ELQDKVTSTE TKVLEMETTV EDLQWDIEKL RKREQKLNKH
310 320 330 340 350
LAEALEQLNS GYYVSGSSTG FQGGQITLSM QKFEMLNAEL EENQELANSR
360 370 380 390 400
MAELEKLQAE LQGAVRTNER LKVALRSLPE EVVRETGEYR MLQAQFSLLY
410 420 430 440 450
NESLQVKTQL DEARGLLLAS KNSHLRHIEH MESDELGLQK KLRTEVIQLE
460 470 480 490 500
DTLAQVRKEY EMLRIEFEQN LAANEQAGPI NREMRHLISS LQNHNHQLKG
510 520 530 540 550
DAQRYKRKLR EVQAEIGKLR AQASGSSHCG PNLSHPDDSG LNALAPGKED
560 570 580 590 600
SGPGPGGTPD SKKELALVAG ATSVASSVKK EELVSSEDDA QALAPGTQGL
610 620 630 640 650
PSRGREPEAR PKRELREREG PSLGPPPAAS TLSRADREKA KAEEARRKES
660 670 680 690 700
ELLKGLRAEL KKAQESQKEM KLLLDMYKSA PKEQRDKVQL MAAERKAKAE
710 720 730 740 750
VDELRSRIRD LEERDRRESK KIADEDALRR IRQAEEQIEH LQRKLGATKQ
760 770 780 790 800
EEEALLSEMD VTGQAFEDMQ EQNGRLLQQL REKDDANFKL MSERIKANQI
810 820 830 840 850
HKLLREEKDE LGEQVLGLKS QVDAQLLTVQ KLEEKERALQ GSLGGVEKEL
860 870 880 890 900
TLRSQALELN KRKAVEAAQL AEDLKVQLEH VQTRLREIQP CLAESRAARE
910 920 930 940 950
KESFNLKRAQ EDISRLRRKL EKQRKVEVYA DADEILQEEI KEYKARLTCP
960 970 980 990 1000
CCNTRKKDAV LTKCFHVFCF ECVRGRYEAR QRKCPKCNAA FGAHDFHRVY

IS
Length:1,002
Mass (Da):113,841
Last modified:March 1, 2003 - v1
Checksum:iEFF371C06B52D231
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF352815 mRNA. Translation: AAN16401.1.
RefSeqiNP_703201.1. NM_153471.1.
UniGeneiRn.162466.

Genome annotation databases

EnsembliENSRNOT00000025499; ENSRNOP00000025500; ENSRNOG00000018840.
GeneIDi266712.
KEGGirno:266712.
UCSCiRGD:628638. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF352815 mRNA. Translation: AAN16401.1.
RefSeqiNP_703201.1. NM_153471.1.
UniGeneiRn.162466.

3D structure databases

ProteinModelPortaliQ8CJB9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CJB9. 2 interactions.
STRINGi10116.ENSRNOP00000025500.

PTM databases

iPTMnetiQ8CJB9.
PhosphoSiteiQ8CJB9.

Proteomic databases

PaxDbiQ8CJB9.
PRIDEiQ8CJB9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025499; ENSRNOP00000025500; ENSRNOG00000018840.
GeneIDi266712.
KEGGirno:266712.
UCSCiRGD:628638. rat.

Organism-specific databases

CTDi9810.
RGDi628638. Rnf40.

Phylogenomic databases

eggNOGiKOG0978. Eukaryota.
ENOG410Y5C6. LUCA.
GeneTreeiENSGT00390000002866.
HOGENOMiHOG000231526.
HOVERGENiHBG080312.
InParanoidiQ8CJB9.
KOiK10696.
OMAiPNLGHPE.
OrthoDBiEOG7DVD99.
PhylomeDBiQ8CJB9.
TreeFamiTF323183.

Enzyme and pathway databases

UniPathwayiUPA00143.
BRENDAi2.3.2.B10. 5301.
6.3.2.19. 5301.

Miscellaneous databases

PROiQ8CJB9.

Gene expression databases

GenevisibleiQ8CJB9. RN.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Staring, a novel E3 ubiquitin-protein ligase that targets syntaxin 1 for degradation."
    Chin L.-S., Vavalle J.P., Li L.
    J. Biol. Chem. 277:35071-35079(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], POSSIBLE FUNCTION, TISSUE SPECIFICITY, POSSIBLE INTERACTION WITH STX1A.
    Strain: Sprague-Dawley.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-585 AND SER-586, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBRE1B_RAT
AccessioniPrimary (citable) accession number: Q8CJB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be cytoplasmic and membrane-associated and to mediate ubiquitination and subsequent degradation of STX1A.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.