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Q8CJ52

- PROM2_RAT

UniProt

Q8CJ52 - PROM2_RAT

Protein

Prominin-2

Gene

Prom2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. cholesterol binding Source: UniProt
    2. growth factor activity Source: RGD
    3. transmembrane signaling receptor activity Source: RGD

    GO - Biological processi

    1. signal transduction Source: GOC

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prominin-2
    Short name:
    PROM-2
    Alternative name(s):
    Prominin-like protein 2
    Short name:
    rPROML2
    Testosterone-regulated prominin-related protein
    Gene namesi
    Name:Prom2
    Synonyms:Proml2, Trprp
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621435. Prom2.

    Subcellular locationi

    Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Cell projectionmicrovillus membrane; Multi-pass membrane protein. Cell projectioncilium membrane; Multi-pass membrane protein
    Note: Localizes to the apical and basolateral membranes of epithelial cells. Associates with membrane in a cholesterol-dependent manner. Colocalizes with PROM1.

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. basolateral plasma membrane Source: UniProtKB-SubCell
    3. cell surface Source: UniProt
    4. ciliary membrane Source: UniProtKB-SubCell
    5. cilium Source: UniProt
    6. cytoplasmic vesicle Source: UniProt
    7. integral component of plasma membrane Source: UniProt
    8. microspike Source: UniProt
    9. microvillus Source: UniProt
    10. microvillus membrane Source: UniProtKB-SubCell
    11. nuclear envelope Source: RGD
    12. prominosome Source: UniProt

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cilium, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 834807Prominin-2PRO_0000331241Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8CJ52.
    PRIDEiQ8CJ52.

    Expressioni

    Tissue specificityi

    In the ventral prostate, expressed in glandular epithelial cells.1 Publication

    Inductioni

    Up-regulated by androgen.1 Publication

    Gene expression databases

    GenevestigatoriQ8CJ52.

    Interactioni

    Subunit structurei

    Binds to cholesterol.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000020354.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini28 – 10679ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini128 – 15326CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini175 – 434260ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini456 – 47217CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini494 – 779286ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini801 – 83434CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei107 – 12721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei154 – 17421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei435 – 45521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei473 – 49321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei780 – 80021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili637 – 67438Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prominin family.Curated

    Keywords - Domaini

    Coiled coil, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG322325.
    HOGENOMiHOG000115705.
    HOVERGENiHBG101108.
    InParanoidiQ8CJ52.
    KOiK15602.
    PhylomeDBiQ8CJ52.

    Family and domain databases

    InterProiIPR008795. Prominin.
    [Graphical view]
    PANTHERiPTHR22730. PTHR22730. 1 hit.
    PfamiPF05478. Prominin. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8CJ52-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTRTLDLMVP LLGLSLGLAL SLPRAVAADC GSLGRVEHLA FAPVPGTEPT    50
    APRVRAPWPL DSLYGTVRRF LSVVQLNPFP AELIKTLLND PSSVKTDEVV 100
    RYEAGYVVCA VIAGLYLLLV PITGLCFCCC RCRRRCGGRV KTEHKAMACE 150
    RGTLMTFLLL TTLMLLIGMV CAFATNQFTH SQTGPSVEAV PETLLSLRGL 200
    VSDVPKKLQA VADQFSSPQK QVSKDLDGVG ENLGNIIHNQ LKSTVYPVLA 250
    SVHGLGQALQ VSIDHLQSVN ATSVELREGQ QHLGPPVQAH RERLLALLQE 300
    SWCHENCKGA LSQASALQLG ADFSQMPPVD DVLHRLQGVP EANFSSMVQE 350
    ENATFNNLPI LVHMQMVSVV KDLKKALAEQ PEGVRMLAQA FPGSEATSRW 400
    SQALEGLEQR SRPYLQDVQR YETYRWILGC VLCSAILLVV ICNLLGLSLG 450
    IWGLFAREDP SHSETKGEAG ARFLMAGVAF SFLFAAPLIL LVFATFLVGG 500
    NVQTLVCRSW ESGELYEFVD TPGNLPPSMN LSYLLGLRKN ISVFQAYRQC 550
    KAGTALWKVL QLNDSYDLDK HLDIKQYTHE LQQELQSFKV DLKDLDLLNP 600
    AARQNLEALQ SSGLEKIHYR DFLVQIQKPV VKTDMGQLAK ELEGLAQAQN 650
    ESLRRQQLQE EARELRSLYQ EKVVPQENLV AKLNPSIRVL ESSAPKLQVN 700
    TSDLLDIVAR LKGELPAQLN HILRNATECF LTREMGYFSQ YVTWVREEVT 750
    QHIATCQPFS KALDDGHMIL CDMMANPWNA FWFCLGWCTF FLIPSIIFAV 800
    KTSKYFRPIR KRLSSTSSEE TQLFHIPRVT SLKL 834
    Length:834
    Mass (Da):92,841
    Last modified:March 1, 2003 - v1
    Checksum:iCE1A53DB5CBEF57A
    GO

    Sequence cautioni

    The sequence AAM03109.1 differs from that shown. Reason: Frameshift at position 775.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti217 – 2171S → L in AAM03109. (PubMed:12446606)Curated
    Sequence conflicti534 – 5341L → I in AAM03109. (PubMed:12446606)Curated
    Sequence conflicti580 – 5801E → K in AAM03109. (PubMed:12446606)Curated
    Sequence conflicti649 – 6491Q → H in AAM03109. (PubMed:12446606)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF486828 mRNA. Translation: AAM03109.1. Frameshift.
    AF508942 mRNA. Translation: AAN63818.1.
    RefSeqiNP_620212.1. NM_138857.1.
    UniGeneiRn.82155.

    Genome annotation databases

    GeneIDi192211.
    KEGGirno:192211.
    UCSCiRGD:621435. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF486828 mRNA. Translation: AAM03109.1 . Frameshift.
    AF508942 mRNA. Translation: AAN63818.1 .
    RefSeqi NP_620212.1. NM_138857.1.
    UniGenei Rn.82155.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000020354.

    Proteomic databases

    PaxDbi Q8CJ52.
    PRIDEi Q8CJ52.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 192211.
    KEGGi rno:192211.
    UCSCi RGD:621435. rat.

    Organism-specific databases

    CTDi 150696.
    RGDi 621435. Prom2.

    Phylogenomic databases

    eggNOGi NOG322325.
    HOGENOMi HOG000115705.
    HOVERGENi HBG101108.
    InParanoidi Q8CJ52.
    KOi K15602.
    PhylomeDBi Q8CJ52.

    Miscellaneous databases

    NextBioi 622797.
    PROi Q8CJ52.

    Gene expression databases

    Genevestigatori Q8CJ52.

    Family and domain databases

    InterProi IPR008795. Prominin.
    [Graphical view ]
    PANTHERi PTHR22730. PTHR22730. 1 hit.
    Pfami PF05478. Prominin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a novel testosterone-regulated prominin-like gene in the rat ventral prostate."
      Zhang Q., Haleem R., Cai X., Wang Z.
      Endocrinology 143:4788-4796(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
    2. "Characterization of prominin-2, a new member of the prominin family of pentaspan membrane glycoproteins."
      Fargeas C.A., Florek M., Huttner W.B., Corbeil D.
      J. Biol. Chem. 278:8586-8596(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    3. "AC133 antigen, CD133, prominin-1, prominin-2, etc.: prominin family gene products in need of a rational nomenclature."
      Fargeas C.A., Corbeil D., Huttner W.B.
      Stem Cells 21:506-508(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    4. "Prominin-2 is a cholesterol-binding protein associated with apical and basolateral plasmalemmal protrusions in polarized epithelial cells and released into urine."
      Florek M., Bauer N., Janich P., Wilsch-Braeuninger M., Fargeas C.A., Marzesco A.-M., Ehninger G., Thiele C., Huttner W.B., Corbeil D.
      Cell Tissue Res. 328:31-47(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHOLESTEROL, GLYCOSYLATION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPROM2_RAT
    AccessioniPrimary (citable) accession number: Q8CJ52
    Secondary accession number(s): Q8R4B6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3