Prominin-2 precursor - Rattus norvegicus (Rat)

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Q8CJ52 (PROM2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 60. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Prominin-2
Short name=PROM-2

Alternative name(s):
Prominin-like protein 2
Short name=rPROML2
Testosterone-regulated prominin-related protein

Gene names

Name:	Prom2
Synonyms:	Proml2, Trprp

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Binds to cholesterol. Ref.4
Subcellular location	Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Cell projection › microvillus membrane; Multi-pass membrane protein. Cell projection › cilium membrane; Multi-pass membrane protein. Note: Localizes to the apical and basolateral membranes of epithelial cells. Associates with membrane in a cholesterol-dependent manner. Colocalizes with PROM1. Ref.2 Ref.4
Tissue specificity	In the ventral prostate, expressed in glandular epithelial cells. Ref.1
Induction	Up-regulated by androgen. Ref.1
Post-translational modification	Glycosylated. Ref.4
Sequence similarities	Belongs to the prominin family.
Sequence caution	The sequence AAM03109.1 differs from that shown. Reason: Frameshift at position 775.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Cilium Membrane
Domain	Coiled coil Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cilium membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microvillus membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear envelope Traceable author statement Ref.1. Source: RGD
Molecular_function	growth factor activity Traceable author statement Ref.1. Source: RGD transmembrane signaling receptor activity Traceable author statement Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

Potential

Chain

28 – 834

807

Prominin-2

PRO_0000331241

Regions

Topological domain

28 – 106

Extracellular Potential

Transmembrane

107 – 127

Helical; Potential

Topological domain

128 – 153

Cytoplasmic Potential

Transmembrane

154 – 174

Helical; Potential

Topological domain

175 – 434

260

Extracellular Potential

Transmembrane

435 – 455

Helical; Potential

Topological domain

456 – 472

Cytoplasmic Potential

Transmembrane

473 – 493

Helical; Potential

Topological domain

494 – 779

286

Extracellular Potential

Transmembrane

780 – 800

Helical; Potential

Topological domain

801 – 834

Cytoplasmic Potential

Coiled coil

637 – 674

Potential

Amino acid modifications

Glycosylation

270

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

217

S → L in AAM03109. Ref.1

Sequence conflict

534

L → I in AAM03109. Ref.1

Sequence conflict

580

E → K in AAM03109. Ref.1

Sequence conflict

649

Q → H in AAM03109. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q8CJ52 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: CE1A53DB5CBEF57A
Show »

FASTA

834

92,841

        10         20         30         40         50         60 
MTRTLDLMVP LLGLSLGLAL SLPRAVAADC GSLGRVEHLA FAPVPGTEPT APRVRAPWPL 

        70         80         90        100        110        120 
DSLYGTVRRF LSVVQLNPFP AELIKTLLND PSSVKTDEVV RYEAGYVVCA VIAGLYLLLV 

       130        140        150        160        170        180 
PITGLCFCCC RCRRRCGGRV KTEHKAMACE RGTLMTFLLL TTLMLLIGMV CAFATNQFTH 

       190        200        210        220        230        240 
SQTGPSVEAV PETLLSLRGL VSDVPKKLQA VADQFSSPQK QVSKDLDGVG ENLGNIIHNQ 

       250        260        270        280        290        300 
LKSTVYPVLA SVHGLGQALQ VSIDHLQSVN ATSVELREGQ QHLGPPVQAH RERLLALLQE 

       310        320        330        340        350        360 
SWCHENCKGA LSQASALQLG ADFSQMPPVD DVLHRLQGVP EANFSSMVQE ENATFNNLPI 

       370        380        390        400        410        420 
LVHMQMVSVV KDLKKALAEQ PEGVRMLAQA FPGSEATSRW SQALEGLEQR SRPYLQDVQR 

       430        440        450        460        470        480 
YETYRWILGC VLCSAILLVV ICNLLGLSLG IWGLFAREDP SHSETKGEAG ARFLMAGVAF 

       490        500        510        520        530        540 
SFLFAAPLIL LVFATFLVGG NVQTLVCRSW ESGELYEFVD TPGNLPPSMN LSYLLGLRKN 

       550        560        570        580        590        600 
ISVFQAYRQC KAGTALWKVL QLNDSYDLDK HLDIKQYTHE LQQELQSFKV DLKDLDLLNP 

       610        620        630        640        650        660 
AARQNLEALQ SSGLEKIHYR DFLVQIQKPV VKTDMGQLAK ELEGLAQAQN ESLRRQQLQE 

       670        680        690        700        710        720 
EARELRSLYQ EKVVPQENLV AKLNPSIRVL ESSAPKLQVN TSDLLDIVAR LKGELPAQLN 

       730        740        750        760        770        780 
HILRNATECF LTREMGYFSQ YVTWVREEVT QHIATCQPFS KALDDGHMIL CDMMANPWNA 

       790        800        810        820        830 
FWFCLGWCTF FLIPSIIFAV KTSKYFRPIR KRLSSTSSEE TQLFHIPRVT SLKL

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References

[1]	"Identification and characterization of a novel testosterone-regulated prominin-like gene in the rat ventral prostate." Zhang Q., Haleem R., Cai X., Wang Z. Endocrinology 143:4788-4796(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY. Strain: Sprague-Dawley.
[2]	"Characterization of prominin-2, a new member of the prominin family of pentaspan membrane glycoproteins." Fargeas C.A., Florek M., Huttner W.B., Corbeil D. J. Biol. Chem. 278:8586-8596(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION. Strain: Sprague-Dawley. Tissue: Kidney.
[3]	"AC133 antigen, CD133, prominin-1, prominin-2, etc.: prominin family gene products in need of a rational nomenclature." Fargeas C.A., Corbeil D., Huttner W.B. Stem Cells 21:506-508(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Kidney.
[4]	"Prominin-2 is a cholesterol-binding protein associated with apical and basolateral plasmalemmal protrusions in polarized epithelial cells and released into urine." Florek M., Bauer N., Janich P., Wilsch-Braeuninger M., Fargeas C.A., Marzesco A.-M., Ehninger G., Thiele C., Huttner W.B., Corbeil D. Cell Tissue Res. 328:31-47(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CHOLESTEROL, GLYCOSYLATION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF486828 mRNA. Translation: AAM03109.1. Frameshift. AF508942 mRNA. Translation: AAN63818.1.
IPI	IPI00560623.
RefSeq	NP_620212.1. NM_138857.1.
UniGene	Rn.82155.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000020354.
Proteomic databases
PaxDb	Q8CJ52.
PRIDE	Q8CJ52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	192211.
KEGG	rno:192211.
UCSC	RGD:621435. rat.
Organism-specific databases
CTD	150696.
RGD	621435. Prom2.
Phylogenomic databases
eggNOG	NOG322325.
HOGENOM	HOG000115705.
HOVERGEN	HBG101108.
InParanoid	Q8CJ52.
KO	K15602.
OrthoDB	EOG4RV2QS.
Gene expression databases
ArrayExpress	Q8CJ52.
Genevestigator	Q8CJ52.
Family and domain databases
InterPro	IPR008795. Prominin. [Graphical view]
PANTHER	PTHR22730. PTHR22730. 1 hit.
Pfam	PF05478. Prominin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	622797.

Entry information

Entry name

PROM2_RAT

Accession

Primary (citable) accession number: Q8CJ52
Secondary accession number(s): Q8R4B6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents