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Q8CJ40

- CROCC_MOUSE

UniProt

Q8CJ40 - CROCC_MOUSE

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Protein
Rootletin
Gene
Crocc, Kiaa0445
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. Contributes to centrosome cohesion before mitosis.By similarity1 Publication

GO - Molecular functioni

  1. kinesin binding Source: MGI
  2. structural molecule activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell projection organization Source: UniProtKB-KW
  3. centrosome organization Source: UniProtKB
  4. protein localization Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Rootletin
Alternative name(s):
Ciliary rootlet coiled-coil protein
Gene namesi
Name:Crocc
Synonyms:Kiaa0445
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:3529431. Crocc.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole
Note: In ciliated cells, associated with ciliary rootlets. In non-ciliated cells, localized between, around and at the proximal ends of the centrioles. Dissociates from the centrioles at the onset of mitosis and reassociates with them at anaphase.2 Publications

GO - Cellular componenti

  1. actin cytoskeleton Source: Ensembl
  2. centriole Source: UniProtKB
  3. ciliary rootlet Source: MGI
  4. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Mice have no ciliary rootlets in ciliated cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20092009Rootletin
PRO_0000239944Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1453 – 14531Phosphoserine By similarity

Post-translational modificationi

Phosphorylated by NEK2 which may regulate its association with centrosomes.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8CJ40.
PaxDbiQ8CJ40.
PRIDEiQ8CJ40.

PTM databases

PhosphoSiteiQ8CJ40.

Expressioni

Tissue specificityi

Highest expression detected in photoreceptor cells of retina. Expressed at lower levels in brain, trachea and kidney. Detected in all major ciliated epithelia. During embryonic development, enriched along the apical domains of neuroepithelium in brain ventricular zone, in primordia of retinal pigment epithelia and in neural retina.1 Publication

Gene expression databases

ArrayExpressiQ8CJ40.
BgeeiQ8CJ40.
CleanExiMM_CROCC.
GenevestigatoriQ8CJ40.

Interactioni

Subunit structurei

Homopolymer. Interacts with KLC3, NEK2 and the N-terminus of CEP250.By similarity2 Publications

Protein-protein interaction databases

BioGridi231046. 5 interactions.
IntActiQ8CJ40. 1 interaction.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili74 – 265192 Reviewed prediction
Add
BLAST
Coiled coili346 – 43893 Reviewed prediction
Add
BLAST
Coiled coili550 – 1058509 Reviewed prediction
Add
BLAST
Coiled coili1091 – 1439349 Reviewed prediction
Add
BLAST
Coiled coili1498 – 1697200 Reviewed prediction
Add
BLAST
Coiled coili1744 – 1998255 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the rootletin family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00730000110800.
HOGENOMiHOG000231187.
HOVERGENiHBG080162.
InParanoidiA2AA81.
KOiK16469.
OMAiYKKRCSE.
OrthoDBiEOG7W6WJX.
TreeFamiTF101138.

Family and domain databases

InterProiIPR026733. Rootletin.
[Graphical view]
PANTHERiPTHR23159:SF11. PTHR23159:SF11. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: Q8CJ40-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSLGLAGSLQ AQLALEIVIQ SLENCVLGPN QEKSLSVQNR VQDFQGASLL     50
VCAREVIASN LSRPETPAPL QVPEMASLLS LQEENQLLQQ ELSRVEDLLA 100
QSRAERDELA IKYNAVNERL EQAVRLETGE LEAQEPRGLV RQSVELRRQL 150
QEEQSSYRRK LQAYQEGQQR QAQLVQRLQA KILQYKKQCS ELEKQLMDRS 200
TELEQQRLRD TEHSQDLDSA LLRLEEEQQR SASLAQVNAM LREQLDQANL 250
ANQALSEDIR KVTSDWTRSC KELEQREAVW RREEESFNTY FSSEHSRLLR 300
LWRQVMGLRR QASEVKMGTE RDLLQLGGEL VRTSRAVQEL GLGLSASLHR 350
AESKAEAALE KQKLLQAQLE EQLQAKLLRE KDLAQLQVQS DLDKADLSAR 400
VTELALSVEH LQNQNSEKDQ VNRTLSDKLE ALESLRLQEQ TTLDTEDGEG 450
LQQTLRDLAQ AALSDTESGV QLSSSERTAD TSDGSLRGFS GQRTPTPPRH 500
SPGRGRSPRR GLSPACSDSS TLTLIHSALH KRQLQVQDMR GRYEASQELL 550
GSVRKQLSDS EGERRGLEEQ LQRLRDQTAA SAQAQEDAQR EAQRLRSANE 600
LLSREKGNLT HSLQVTQQQA KELRQELEKL QAAQEELKRQ HNQLEDAQED 650
SVQEGARARR ELERSHRQLE QLEVKRSGLT KELVEVREAL SCAILQRDVL 700
QTEKAEVAEA LTKAEAGRAQ LELSLTKLRA EEASLRDSLS KMSALNESLA 750
QDKLELNRLI AQLEEEKVAL LGRQQQAEHA TTMAVEKQEL LEQLRLEQEV 800
ERQGLQGSLC VAEQAREALE QQILVLRSER SHLQEQLAQL SRQLSGRDQE 850
LEQALRESQR QVEALERAAR EKEAMAKERA GLAVKLAAAE REGRTLSEEA 900
IRLRLEKEAL ESSLFDVQRQ LAQLEARREQ LEADSQALLL AKETLTGELA 950
GLRQQVTSTE EKAALDKELM TQKLVQAERE AQASLREQRA AHEEDLQRLQ 1000
HEKEAAWREL QAERAQLQGQ LQQEREELLA RMEAEKEELS KEIAALQQER 1050
DEGLLLAESE KQQALSLKES EKTALSEKLM GTRHSLAAIS LEMERQKRDA 1100
QSRQEQDRNT LNALTSELRD LRAQLEEATA AHAQTVKELE ERTGNLGRQR 1150
EACMREAEEL RTQLRVLEDT RDGLRRELLE AQRKGRDSQD SSEAHRQEAS 1200
ELRRSLSEGA KEREALRRSN EELRSAVKKA ESERISLKLA NEDKEQKLAL 1250
LEEARVSVAK EAGELRASLQ EVERSRLEAR RELQELRRQM KTLDSDNGRL 1300
GRELADLQGR LALGERTEKE SRREALGLRQ RLLKGESSLE ALKQELQGSQ 1350
RKLQEQEAEF RARERGLLGS LEEARGAEKR LLDSARSLEL RLEAVRAETS 1400
ELGLRLSAAE GRAQGLEVEL ARVEAQRRVA EAQLGGLRSA LRRGLGLGRV 1450
SSSPAREAPA GGSGDGLSSP SPLEYSPRSQ PPSPGLIASP APPDLDPEAV 1500
RDALRDFLQE LRSAQRERDE LKVQTSTLSQ QLVEMEAERD HAASRAKQLQ 1550
KAVAESEEAW RSADRRLSGA QAELALQEES VRRSKRECRA TLDQMAVLER 1600
SLQATESELR ASQEKVSKMK ATEAKLESDK RRLKEVLDAS ESRSIKLELQ 1650
RRALEGELQR SRLGLGDREA HAQALQDRVD SLQRQVADSE VKAGTLQLTV 1700
ERLSGALAKV EESEGNLRSK VQSLTDALTQ SSASLSSTQD KNLHLQKALS 1750
TCEHDRQVLQ ERLDAARQAL SEARRQSSSL GEQVQTLRGE LASLELQRGD 1800
AEGQLQQLQQ ALRQRQEGEA MALRSVQKLQ EERRLLQERL GSLQRALAQL 1850
EAEKRDLERS ALQFDKDRVA LRKTLDKVER EKLRSHEDTL RLNAERGRLD 1900
RTLTGAELDL AEAQQQIQHL EAQVDVALEG NHNPVQPEAG EQQLELQQEV 1950
ERLRSAQVQT ERTLEARERA HRQRVSGLEE QVSTLKAQLH QELRRSSASV 2000
SLPPGTPEK 2009
Length:2,009
Mass (Da):226,945
Last modified:July 27, 2011 - v2
Checksum:i62D942401C1085DA
GO
Isoform 21 Publication (identifier: Q8CJ40-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.
     120-209: Missing.

Note: No experimental confirmation available.

Show »
Length:1,845
Mass (Da):208,269
Checksum:i12C8A15759C8B64D
GO
Isoform 31 Publication (identifier: Q8CJ40-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1136-1156: Missing.

Note: No experimental confirmation available.

Show »
Length:1,988
Mass (Da):224,458
Checksum:i0F53D354E8559A2B
GO

Sequence cautioni

The sequence AAH27090.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7474Missing in isoform 2. 1 Publication
VSP_052066Add
BLAST
Alternative sequencei120 – 20990Missing in isoform 2. 1 Publication
VSP_052067Add
BLAST
Alternative sequencei1136 – 115621Missing in isoform 3. 1 Publication
VSP_052068Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti289 – 2891T → A in AAN73044. 1 Publication
Sequence conflicti289 – 2891T → A in AAH54054. 1 Publication
Sequence conflicti360 – 3601E → G in AAH54054. 1 Publication
Sequence conflicti811 – 8111V → M in AAH54054. 1 Publication
Sequence conflicti1041 – 10411K → E in AAH54054. 1 Publication
Sequence conflicti1346 – 13461L → V in BAC65567. 1 Publication
Sequence conflicti1864 – 18641F → L in AAH54054. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF527975 mRNA. Translation: AAN73044.1.
AL645625 Genomic DNA. Translation: CAM16757.1.
BC027090 mRNA. Translation: AAH27090.1. Different initiation.
BC054054 mRNA. Translation: AAH54054.1.
AK122285 Transcribed RNA. Translation: BAC65567.3.
CCDSiCCDS18861.1. [Q8CJ40-1]
CCDS51346.1. [Q8CJ40-2]
RefSeqiNP_001139430.1. NM_001145958.1. [Q8CJ40-2]
NP_742120.2. NM_172122.2. [Q8CJ40-1]
UniGeneiMm.80685.

Genome annotation databases

EnsembliENSMUST00000040222; ENSMUSP00000037679; ENSMUSG00000040860. [Q8CJ40-2]
ENSMUST00000097816; ENSMUSP00000095425; ENSMUSG00000040860. [Q8CJ40-2]
ENSMUST00000102491; ENSMUSP00000099549; ENSMUSG00000040860. [Q8CJ40-1]
ENSMUST00000168157; ENSMUSP00000126543; ENSMUSG00000040860. [Q8CJ40-2]
GeneIDi230872.
KEGGimmu:230872.
UCSCiuc008vns.2. mouse. [Q8CJ40-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF527975 mRNA. Translation: AAN73044.1 .
AL645625 Genomic DNA. Translation: CAM16757.1 .
BC027090 mRNA. Translation: AAH27090.1 . Different initiation.
BC054054 mRNA. Translation: AAH54054.1 .
AK122285 Transcribed RNA. Translation: BAC65567.3 .
CCDSi CCDS18861.1. [Q8CJ40-1 ]
CCDS51346.1. [Q8CJ40-2 ]
RefSeqi NP_001139430.1. NM_001145958.1. [Q8CJ40-2 ]
NP_742120.2. NM_172122.2. [Q8CJ40-1 ]
UniGenei Mm.80685.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

BioGridi 231046. 5 interactions.
IntActi Q8CJ40. 1 interaction.

PTM databases

PhosphoSitei Q8CJ40.

Proteomic databases

MaxQBi Q8CJ40.
PaxDbi Q8CJ40.
PRIDEi Q8CJ40.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000040222 ; ENSMUSP00000037679 ; ENSMUSG00000040860 . [Q8CJ40-2 ]
ENSMUST00000097816 ; ENSMUSP00000095425 ; ENSMUSG00000040860 . [Q8CJ40-2 ]
ENSMUST00000102491 ; ENSMUSP00000099549 ; ENSMUSG00000040860 . [Q8CJ40-1 ]
ENSMUST00000168157 ; ENSMUSP00000126543 ; ENSMUSG00000040860 . [Q8CJ40-2 ]
GeneIDi 230872.
KEGGi mmu:230872.
UCSCi uc008vns.2. mouse. [Q8CJ40-1 ]

Organism-specific databases

CTDi 9696.
MGIi MGI:3529431. Crocc.
Rougei Search...

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00730000110800.
HOGENOMi HOG000231187.
HOVERGENi HBG080162.
InParanoidi A2AA81.
KOi K16469.
OMAi YKKRCSE.
OrthoDBi EOG7W6WJX.
TreeFami TF101138.

Miscellaneous databases

ChiTaRSi CROCC. mouse.
NextBioi 380242.
PROi Q8CJ40.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8CJ40.
Bgeei Q8CJ40.
CleanExi MM_CROCC.
Genevestigatori Q8CJ40.

Family and domain databases

InterProi IPR026733. Rootletin.
[Graphical view ]
PANTHERi PTHR23159:SF11. PTHR23159:SF11. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rootletin, a novel coiled-coil protein, is a structural component of the ciliary rootlet."
    Yang J., Liu X., Yue G., Adamian M., Bulgakov O., Li T.
    J. Cell Biol. 159:431-440(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH KLC3.
    Strain: C57BL/6.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6 and FVB/N.
    Tissue: Colon and Retina.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 391-2009 (ISOFORM 3).
    Tissue: Brain.
  5. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1261-1274, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  6. "The ciliary rootlet maintains long-term stability of sensory cilia."
    Yang J., Gao J., Adamian M., Wen X.-H., Pawlyk B., Zhang L., Sanderson M.J., Zuo J., Makino C.L., Li T.
    Mol. Cell. Biol. 25:4129-4137(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Rootletin interacts with C-Nap1 and may function as a physical linker between the pair of centrioles/basal bodies in cells."
    Yang J., Adamian M., Li T.
    Mol. Biol. Cell 17:1033-1040(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CEP250.

Entry informationi

Entry nameiCROCC_MOUSE
AccessioniPrimary (citable) accession number: Q8CJ40
Secondary accession number(s): A2AA81
, Q7TQL2, Q80U01, Q8R0B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi