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Q8CJ40 (CROCC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rootletin
Alternative name(s):
Ciliary rootlet coiled-coil protein
Gene names
Name:Crocc
Synonyms:Kiaa0445
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2009 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. Contributes to centrosome cohesion before mitosis. Ref.1 UniProtKB Q5TZA2

Subunit structure

Homopolymer. Interacts with KLC3, NEK2 and the N-terminus of CEP250. Ref.1 Ref.7 UniProtKB Q5TZA2

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole. Note: In ciliated cells, associated with ciliary rootlets. In non-ciliated cells, localized between, around and at the proximal ends of the centrioles. Dissociates from the centrioles at the onset of mitosis and reassociates with them at anaphase. Ref.1 Ref.7

Tissue specificity

Highest expression detected in photoreceptor cells of retina. Expressed at lower levels in brain, trachea and kidney. Detected in all major ciliated epithelia. During embryonic development, enriched along the apical domains of neuroepithelium in brain ventricular zone, in primordia of retinal pigment epithelia and in neural retina. Ref.1

Post-translational modification

Phosphorylated by NEK2 which may regulate its association with centrosomes. UniProtKB Q5TZA2

Disruption phenotype

Mice have no ciliary rootlets in ciliated cells. Ref.6

Sequence similarities

Belongs to the rootletin family.

Sequence caution

The sequence AAH27090.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q8CJ40-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.3 (identifier: Q8CJ40-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.
     120-209: Missing.
Note: No experimental confirmation available.
Isoform 3 Ref.4 (identifier: Q8CJ40-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1136-1156: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 20092009Rootletin
PRO_0000239944

Regions

Coiled coil74 – 265192 Potential
Coiled coil346 – 43893 Potential
Coiled coil550 – 1058509 Potential
Coiled coil1091 – 1439349 Potential
Coiled coil1498 – 1697200 Potential
Coiled coil1744 – 1998255 Potential

Amino acid modifications

Modified residue14531Phosphoserine By similarity

Natural variations

Alternative sequence1 – 7474Missing in isoform 2. Ref.3
VSP_052066
Alternative sequence120 – 20990Missing in isoform 2. Ref.3
VSP_052067
Alternative sequence1136 – 115621Missing in isoform 3. Ref.4
VSP_052068

Experimental info

Sequence conflict2891T → A in AAN73044. Ref.1
Sequence conflict2891T → A in AAH54054. Ref.3
Sequence conflict3601E → G in AAH54054. Ref.3
Sequence conflict8111V → M in AAH54054. Ref.3
Sequence conflict10411K → E in AAH54054. Ref.3
Sequence conflict13461L → V in BAC65567. Ref.3
Sequence conflict18641F → L in AAH54054. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 62D942401C1085DA

FASTA2,009226,945
        10         20         30         40         50         60 
MSLGLAGSLQ AQLALEIVIQ SLENCVLGPN QEKSLSVQNR VQDFQGASLL VCAREVIASN 

        70         80         90        100        110        120 
LSRPETPAPL QVPEMASLLS LQEENQLLQQ ELSRVEDLLA QSRAERDELA IKYNAVNERL 

       130        140        150        160        170        180 
EQAVRLETGE LEAQEPRGLV RQSVELRRQL QEEQSSYRRK LQAYQEGQQR QAQLVQRLQA 

       190        200        210        220        230        240 
KILQYKKQCS ELEKQLMDRS TELEQQRLRD TEHSQDLDSA LLRLEEEQQR SASLAQVNAM 

       250        260        270        280        290        300 
LREQLDQANL ANQALSEDIR KVTSDWTRSC KELEQREAVW RREEESFNTY FSSEHSRLLR 

       310        320        330        340        350        360 
LWRQVMGLRR QASEVKMGTE RDLLQLGGEL VRTSRAVQEL GLGLSASLHR AESKAEAALE 

       370        380        390        400        410        420 
KQKLLQAQLE EQLQAKLLRE KDLAQLQVQS DLDKADLSAR VTELALSVEH LQNQNSEKDQ 

       430        440        450        460        470        480 
VNRTLSDKLE ALESLRLQEQ TTLDTEDGEG LQQTLRDLAQ AALSDTESGV QLSSSERTAD 

       490        500        510        520        530        540 
TSDGSLRGFS GQRTPTPPRH SPGRGRSPRR GLSPACSDSS TLTLIHSALH KRQLQVQDMR 

       550        560        570        580        590        600 
GRYEASQELL GSVRKQLSDS EGERRGLEEQ LQRLRDQTAA SAQAQEDAQR EAQRLRSANE 

       610        620        630        640        650        660 
LLSREKGNLT HSLQVTQQQA KELRQELEKL QAAQEELKRQ HNQLEDAQED SVQEGARARR 

       670        680        690        700        710        720 
ELERSHRQLE QLEVKRSGLT KELVEVREAL SCAILQRDVL QTEKAEVAEA LTKAEAGRAQ 

       730        740        750        760        770        780 
LELSLTKLRA EEASLRDSLS KMSALNESLA QDKLELNRLI AQLEEEKVAL LGRQQQAEHA 

       790        800        810        820        830        840 
TTMAVEKQEL LEQLRLEQEV ERQGLQGSLC VAEQAREALE QQILVLRSER SHLQEQLAQL 

       850        860        870        880        890        900 
SRQLSGRDQE LEQALRESQR QVEALERAAR EKEAMAKERA GLAVKLAAAE REGRTLSEEA 

       910        920        930        940        950        960 
IRLRLEKEAL ESSLFDVQRQ LAQLEARREQ LEADSQALLL AKETLTGELA GLRQQVTSTE 

       970        980        990       1000       1010       1020 
EKAALDKELM TQKLVQAERE AQASLREQRA AHEEDLQRLQ HEKEAAWREL QAERAQLQGQ 

      1030       1040       1050       1060       1070       1080 
LQQEREELLA RMEAEKEELS KEIAALQQER DEGLLLAESE KQQALSLKES EKTALSEKLM 

      1090       1100       1110       1120       1130       1140 
GTRHSLAAIS LEMERQKRDA QSRQEQDRNT LNALTSELRD LRAQLEEATA AHAQTVKELE 

      1150       1160       1170       1180       1190       1200 
ERTGNLGRQR EACMREAEEL RTQLRVLEDT RDGLRRELLE AQRKGRDSQD SSEAHRQEAS 

      1210       1220       1230       1240       1250       1260 
ELRRSLSEGA KEREALRRSN EELRSAVKKA ESERISLKLA NEDKEQKLAL LEEARVSVAK 

      1270       1280       1290       1300       1310       1320 
EAGELRASLQ EVERSRLEAR RELQELRRQM KTLDSDNGRL GRELADLQGR LALGERTEKE 

      1330       1340       1350       1360       1370       1380 
SRREALGLRQ RLLKGESSLE ALKQELQGSQ RKLQEQEAEF RARERGLLGS LEEARGAEKR 

      1390       1400       1410       1420       1430       1440 
LLDSARSLEL RLEAVRAETS ELGLRLSAAE GRAQGLEVEL ARVEAQRRVA EAQLGGLRSA 

      1450       1460       1470       1480       1490       1500 
LRRGLGLGRV SSSPAREAPA GGSGDGLSSP SPLEYSPRSQ PPSPGLIASP APPDLDPEAV 

      1510       1520       1530       1540       1550       1560 
RDALRDFLQE LRSAQRERDE LKVQTSTLSQ QLVEMEAERD HAASRAKQLQ KAVAESEEAW 

      1570       1580       1590       1600       1610       1620 
RSADRRLSGA QAELALQEES VRRSKRECRA TLDQMAVLER SLQATESELR ASQEKVSKMK 

      1630       1640       1650       1660       1670       1680 
ATEAKLESDK RRLKEVLDAS ESRSIKLELQ RRALEGELQR SRLGLGDREA HAQALQDRVD 

      1690       1700       1710       1720       1730       1740 
SLQRQVADSE VKAGTLQLTV ERLSGALAKV EESEGNLRSK VQSLTDALTQ SSASLSSTQD 

      1750       1760       1770       1780       1790       1800 
KNLHLQKALS TCEHDRQVLQ ERLDAARQAL SEARRQSSSL GEQVQTLRGE LASLELQRGD 

      1810       1820       1830       1840       1850       1860 
AEGQLQQLQQ ALRQRQEGEA MALRSVQKLQ EERRLLQERL GSLQRALAQL EAEKRDLERS 

      1870       1880       1890       1900       1910       1920 
ALQFDKDRVA LRKTLDKVER EKLRSHEDTL RLNAERGRLD RTLTGAELDL AEAQQQIQHL 

      1930       1940       1950       1960       1970       1980 
EAQVDVALEG NHNPVQPEAG EQQLELQQEV ERLRSAQVQT ERTLEARERA HRQRVSGLEE 

      1990       2000 
QVSTLKAQLH QELRRSSASV SLPPGTPEK 

« Hide

Isoform 2 [UniParc].

Checksum: 12C8A15759C8B64D
Show »

FASTA1,845208,269
Isoform 3 [UniParc].

Checksum: 0F53D354E8559A2B
Show »

FASTA1,988224,458

References

« Hide 'large scale' references
[1]"Rootletin, a novel coiled-coil protein, is a structural component of the ciliary rootlet."
Yang J., Liu X., Yue G., Adamian M., Bulgakov O., Li T.
J. Cell Biol. 159:431-440(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH KLC3.
Strain: C57BL/6.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6 and FVB/N.
Tissue: Colon and Retina.
[4]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 391-2009 (ISOFORM 3).
Tissue: Brain.
[5]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 1261-1274, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[6]"The ciliary rootlet maintains long-term stability of sensory cilia."
Yang J., Gao J., Adamian M., Wen X.-H., Pawlyk B., Zhang L., Sanderson M.J., Zuo J., Makino C.L., Li T.
Mol. Cell. Biol. 25:4129-4137(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[7]"Rootletin interacts with C-Nap1 and may function as a physical linker between the pair of centrioles/basal bodies in cells."
Yang J., Adamian M., Li T.
Mol. Biol. Cell 17:1033-1040(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CEP250.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF527975 mRNA. Translation: AAN73044.1.
AL645625 Genomic DNA. Translation: CAM16757.1.
BC027090 mRNA. Translation: AAH27090.1. Different initiation.
BC054054 mRNA. Translation: AAH54054.1.
AK122285 Transcribed RNA. Translation: BAC65567.3.
RefSeqNP_001139430.1. NM_001145958.1.
NP_742120.2. NM_172122.2.
UniGeneMm.80685.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid231046. 4 interactions.
IntActQ8CJ40. 1 interaction.

PTM databases

PhosphoSiteQ8CJ40.

Proteomic databases

PaxDbQ8CJ40.
PRIDEQ8CJ40.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000040222; ENSMUSP00000037679; ENSMUSG00000040860. [Q8CJ40-2]
ENSMUST00000097816; ENSMUSP00000095425; ENSMUSG00000040860. [Q8CJ40-2]
ENSMUST00000102491; ENSMUSP00000099549; ENSMUSG00000040860. [Q8CJ40-1]
ENSMUST00000168157; ENSMUSP00000126543; ENSMUSG00000040860. [Q8CJ40-2]
GeneID230872.
KEGGmmu:230872.
UCSCuc008vns.2. mouse. [Q8CJ40-1]

Organism-specific databases

CTD9696.
MGIMGI:3529431. Crocc.
RougeSearch...

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00730000110800.
HOGENOMHOG000231187.
HOVERGENHBG080162.
InParanoidA2AA81.
KOK16469.
OMAATISLEM.
OrthoDBEOG7W6WJX.
TreeFamTF101138.

Gene expression databases

ArrayExpressQ8CJ40.
BgeeQ8CJ40.
CleanExMM_CROCC.
GenevestigatorQ8CJ40.

Family and domain databases

InterProIPR026733. Rootletin.
[Graphical view]
PANTHERPTHR23159:SF5. PTHR23159:SF5. 1 hit.
ProtoNetSearch...

Other

ChiTaRSCROCC. mouse.
NextBio380242.
PROQ8CJ40.
SOURCESearch...

Entry information

Entry nameCROCC_MOUSE
AccessionPrimary (citable) accession number: Q8CJ40
Secondary accession number(s): A2AA81 expand/collapse secondary AC list , Q7TQL2, Q80U01, Q8R0B9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot