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Q8CJ40

- CROCC_MOUSE

UniProt

Q8CJ40 - CROCC_MOUSE

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Protein

Rootletin

Gene

Crocc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. Contributes to centrosome cohesion before mitosis.By similarity1 Publication

GO - Molecular functioni

  1. kinesin binding Source: MGI
  2. structural molecule activity Source: UniProtKB

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell projection organization Source: UniProtKB-KW
  3. centrosome organization Source: UniProtKB
  4. protein localization Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Rootletin
Alternative name(s):
Ciliary rootlet coiled-coil protein
Gene namesi
Name:CroccImported
Synonyms:Kiaa0445Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:3529431. Crocc.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole 2 Publications
Note: In ciliated cells, associated with ciliary rootlets. In non-ciliated cells, localized between, around and at the proximal ends of the centrioles. Dissociates from the centrioles at the onset of mitosis and reassociates with them at anaphase.

GO - Cellular componenti

  1. actin cytoskeleton Source: Ensembl
  2. centriole Source: UniProtKB
  3. ciliary rootlet Source: MGI
  4. extracellular vesicular exosome Source: Ensembl
  5. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Mice have no ciliary rootlets in ciliated cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20092009RootletinPRO_0000239944Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1453 – 14531PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by NEK2 which may regulate its association with centrosomes.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8CJ40.
PaxDbiQ8CJ40.
PRIDEiQ8CJ40.

PTM databases

PhosphoSiteiQ8CJ40.

Expressioni

Tissue specificityi

Highest expression detected in photoreceptor cells of retina. Expressed at lower levels in brain, trachea and kidney. Detected in all major ciliated epithelia. During embryonic development, enriched along the apical domains of neuroepithelium in brain ventricular zone, in primordia of retinal pigment epithelia and in neural retina.1 Publication

Gene expression databases

BgeeiQ8CJ40.
CleanExiMM_CROCC.
ExpressionAtlasiQ8CJ40. baseline and differential.
GenevestigatoriQ8CJ40.

Interactioni

Subunit structurei

Homopolymer. Interacts with KLC3, NEK2 and the N-terminus of CEP250.2 Publications

Protein-protein interaction databases

BioGridi231046. 5 interactions.
IntActiQ8CJ40. 1 interaction.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili74 – 265192Sequence AnalysisAdd
BLAST
Coiled coili346 – 43893Sequence AnalysisAdd
BLAST
Coiled coili550 – 1058509Sequence AnalysisAdd
BLAST
Coiled coili1091 – 1439349Sequence AnalysisAdd
BLAST
Coiled coili1498 – 1697200Sequence AnalysisAdd
BLAST
Coiled coili1744 – 1998255Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the rootletin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00730000110800.
HOGENOMiHOG000231187.
HOVERGENiHBG080162.
InParanoidiQ8CJ40.
KOiK16469.
OMAiYKKRCSE.
OrthoDBiEOG7W6WJX.
TreeFamiTF101138.

Family and domain databases

InterProiIPR026733. Rootletin.
[Graphical view]
PANTHERiPTHR23159:SF11. PTHR23159:SF11. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: Q8CJ40-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLGLAGSLQ AQLALEIVIQ SLENCVLGPN QEKSLSVQNR VQDFQGASLL
60 70 80 90 100
VCAREVIASN LSRPETPAPL QVPEMASLLS LQEENQLLQQ ELSRVEDLLA
110 120 130 140 150
QSRAERDELA IKYNAVNERL EQAVRLETGE LEAQEPRGLV RQSVELRRQL
160 170 180 190 200
QEEQSSYRRK LQAYQEGQQR QAQLVQRLQA KILQYKKQCS ELEKQLMDRS
210 220 230 240 250
TELEQQRLRD TEHSQDLDSA LLRLEEEQQR SASLAQVNAM LREQLDQANL
260 270 280 290 300
ANQALSEDIR KVTSDWTRSC KELEQREAVW RREEESFNTY FSSEHSRLLR
310 320 330 340 350
LWRQVMGLRR QASEVKMGTE RDLLQLGGEL VRTSRAVQEL GLGLSASLHR
360 370 380 390 400
AESKAEAALE KQKLLQAQLE EQLQAKLLRE KDLAQLQVQS DLDKADLSAR
410 420 430 440 450
VTELALSVEH LQNQNSEKDQ VNRTLSDKLE ALESLRLQEQ TTLDTEDGEG
460 470 480 490 500
LQQTLRDLAQ AALSDTESGV QLSSSERTAD TSDGSLRGFS GQRTPTPPRH
510 520 530 540 550
SPGRGRSPRR GLSPACSDSS TLTLIHSALH KRQLQVQDMR GRYEASQELL
560 570 580 590 600
GSVRKQLSDS EGERRGLEEQ LQRLRDQTAA SAQAQEDAQR EAQRLRSANE
610 620 630 640 650
LLSREKGNLT HSLQVTQQQA KELRQELEKL QAAQEELKRQ HNQLEDAQED
660 670 680 690 700
SVQEGARARR ELERSHRQLE QLEVKRSGLT KELVEVREAL SCAILQRDVL
710 720 730 740 750
QTEKAEVAEA LTKAEAGRAQ LELSLTKLRA EEASLRDSLS KMSALNESLA
760 770 780 790 800
QDKLELNRLI AQLEEEKVAL LGRQQQAEHA TTMAVEKQEL LEQLRLEQEV
810 820 830 840 850
ERQGLQGSLC VAEQAREALE QQILVLRSER SHLQEQLAQL SRQLSGRDQE
860 870 880 890 900
LEQALRESQR QVEALERAAR EKEAMAKERA GLAVKLAAAE REGRTLSEEA
910 920 930 940 950
IRLRLEKEAL ESSLFDVQRQ LAQLEARREQ LEADSQALLL AKETLTGELA
960 970 980 990 1000
GLRQQVTSTE EKAALDKELM TQKLVQAERE AQASLREQRA AHEEDLQRLQ
1010 1020 1030 1040 1050
HEKEAAWREL QAERAQLQGQ LQQEREELLA RMEAEKEELS KEIAALQQER
1060 1070 1080 1090 1100
DEGLLLAESE KQQALSLKES EKTALSEKLM GTRHSLAAIS LEMERQKRDA
1110 1120 1130 1140 1150
QSRQEQDRNT LNALTSELRD LRAQLEEATA AHAQTVKELE ERTGNLGRQR
1160 1170 1180 1190 1200
EACMREAEEL RTQLRVLEDT RDGLRRELLE AQRKGRDSQD SSEAHRQEAS
1210 1220 1230 1240 1250
ELRRSLSEGA KEREALRRSN EELRSAVKKA ESERISLKLA NEDKEQKLAL
1260 1270 1280 1290 1300
LEEARVSVAK EAGELRASLQ EVERSRLEAR RELQELRRQM KTLDSDNGRL
1310 1320 1330 1340 1350
GRELADLQGR LALGERTEKE SRREALGLRQ RLLKGESSLE ALKQELQGSQ
1360 1370 1380 1390 1400
RKLQEQEAEF RARERGLLGS LEEARGAEKR LLDSARSLEL RLEAVRAETS
1410 1420 1430 1440 1450
ELGLRLSAAE GRAQGLEVEL ARVEAQRRVA EAQLGGLRSA LRRGLGLGRV
1460 1470 1480 1490 1500
SSSPAREAPA GGSGDGLSSP SPLEYSPRSQ PPSPGLIASP APPDLDPEAV
1510 1520 1530 1540 1550
RDALRDFLQE LRSAQRERDE LKVQTSTLSQ QLVEMEAERD HAASRAKQLQ
1560 1570 1580 1590 1600
KAVAESEEAW RSADRRLSGA QAELALQEES VRRSKRECRA TLDQMAVLER
1610 1620 1630 1640 1650
SLQATESELR ASQEKVSKMK ATEAKLESDK RRLKEVLDAS ESRSIKLELQ
1660 1670 1680 1690 1700
RRALEGELQR SRLGLGDREA HAQALQDRVD SLQRQVADSE VKAGTLQLTV
1710 1720 1730 1740 1750
ERLSGALAKV EESEGNLRSK VQSLTDALTQ SSASLSSTQD KNLHLQKALS
1760 1770 1780 1790 1800
TCEHDRQVLQ ERLDAARQAL SEARRQSSSL GEQVQTLRGE LASLELQRGD
1810 1820 1830 1840 1850
AEGQLQQLQQ ALRQRQEGEA MALRSVQKLQ EERRLLQERL GSLQRALAQL
1860 1870 1880 1890 1900
EAEKRDLERS ALQFDKDRVA LRKTLDKVER EKLRSHEDTL RLNAERGRLD
1910 1920 1930 1940 1950
RTLTGAELDL AEAQQQIQHL EAQVDVALEG NHNPVQPEAG EQQLELQQEV
1960 1970 1980 1990 2000
ERLRSAQVQT ERTLEARERA HRQRVSGLEE QVSTLKAQLH QELRRSSASV

SLPPGTPEK
Length:2,009
Mass (Da):226,945
Last modified:July 27, 2011 - v2
Checksum:i62D942401C1085DA
GO
Isoform 21 Publication (identifier: Q8CJ40-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.
     120-209: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:1,845
Mass (Da):208,269
Checksum:i12C8A15759C8B64D
GO
Isoform 31 Publication (identifier: Q8CJ40-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1136-1156: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:1,988
Mass (Da):224,458
Checksum:i0F53D354E8559A2B
GO

Sequence cautioni

The sequence AAH27090.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti289 – 2891T → A in AAN73044. (PubMed:12427867)Curated
Sequence conflicti289 – 2891T → A in AAH54054. (PubMed:15489334)Curated
Sequence conflicti360 – 3601E → G in AAH54054. (PubMed:15489334)Curated
Sequence conflicti811 – 8111V → M in AAH54054. (PubMed:15489334)Curated
Sequence conflicti1041 – 10411K → E in AAH54054. (PubMed:15489334)Curated
Sequence conflicti1346 – 13461L → V in BAC65567. (PubMed:15489334)Curated
Sequence conflicti1864 – 18641F → L in AAH54054. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7474Missing in isoform 2. 1 PublicationVSP_052066Add
BLAST
Alternative sequencei120 – 20990Missing in isoform 2. 1 PublicationVSP_052067Add
BLAST
Alternative sequencei1136 – 115621Missing in isoform 3. 1 PublicationVSP_052068Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF527975 mRNA. Translation: AAN73044.1.
AL645625 Genomic DNA. Translation: CAM16757.1.
BC027090 mRNA. Translation: AAH27090.1. Different initiation.
BC054054 mRNA. Translation: AAH54054.1.
AK122285 Transcribed RNA. Translation: BAC65567.3.
CCDSiCCDS18861.1. [Q8CJ40-1]
CCDS51346.1. [Q8CJ40-2]
RefSeqiNP_001139430.1. NM_001145958.1. [Q8CJ40-2]
NP_742120.2. NM_172122.2. [Q8CJ40-1]
UniGeneiMm.80685.

Genome annotation databases

EnsembliENSMUST00000040222; ENSMUSP00000037679; ENSMUSG00000040860. [Q8CJ40-2]
ENSMUST00000097816; ENSMUSP00000095425; ENSMUSG00000040860. [Q8CJ40-2]
ENSMUST00000102491; ENSMUSP00000099549; ENSMUSG00000040860. [Q8CJ40-1]
ENSMUST00000168157; ENSMUSP00000126543; ENSMUSG00000040860. [Q8CJ40-2]
GeneIDi230872.
KEGGimmu:230872.
UCSCiuc008vns.2. mouse. [Q8CJ40-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF527975 mRNA. Translation: AAN73044.1 .
AL645625 Genomic DNA. Translation: CAM16757.1 .
BC027090 mRNA. Translation: AAH27090.1 . Different initiation.
BC054054 mRNA. Translation: AAH54054.1 .
AK122285 Transcribed RNA. Translation: BAC65567.3 .
CCDSi CCDS18861.1. [Q8CJ40-1 ]
CCDS51346.1. [Q8CJ40-2 ]
RefSeqi NP_001139430.1. NM_001145958.1. [Q8CJ40-2 ]
NP_742120.2. NM_172122.2. [Q8CJ40-1 ]
UniGenei Mm.80685.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 231046. 5 interactions.
IntActi Q8CJ40. 1 interaction.

PTM databases

PhosphoSitei Q8CJ40.

Proteomic databases

MaxQBi Q8CJ40.
PaxDbi Q8CJ40.
PRIDEi Q8CJ40.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000040222 ; ENSMUSP00000037679 ; ENSMUSG00000040860 . [Q8CJ40-2 ]
ENSMUST00000097816 ; ENSMUSP00000095425 ; ENSMUSG00000040860 . [Q8CJ40-2 ]
ENSMUST00000102491 ; ENSMUSP00000099549 ; ENSMUSG00000040860 . [Q8CJ40-1 ]
ENSMUST00000168157 ; ENSMUSP00000126543 ; ENSMUSG00000040860 . [Q8CJ40-2 ]
GeneIDi 230872.
KEGGi mmu:230872.
UCSCi uc008vns.2. mouse. [Q8CJ40-1 ]

Organism-specific databases

CTDi 9696.
MGIi MGI:3529431. Crocc.
Rougei Search...

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00730000110800.
HOGENOMi HOG000231187.
HOVERGENi HBG080162.
InParanoidi Q8CJ40.
KOi K16469.
OMAi YKKRCSE.
OrthoDBi EOG7W6WJX.
TreeFami TF101138.

Miscellaneous databases

ChiTaRSi CROCC. mouse.
NextBioi 380242.
PROi Q8CJ40.
SOURCEi Search...

Gene expression databases

Bgeei Q8CJ40.
CleanExi MM_CROCC.
ExpressionAtlasi Q8CJ40. baseline and differential.
Genevestigatori Q8CJ40.

Family and domain databases

InterProi IPR026733. Rootletin.
[Graphical view ]
PANTHERi PTHR23159:SF11. PTHR23159:SF11. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rootletin, a novel coiled-coil protein, is a structural component of the ciliary rootlet."
    Yang J., Liu X., Yue G., Adamian M., Bulgakov O., Li T.
    J. Cell Biol. 159:431-440(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH KLC3.
    Strain: C57BL/6Imported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6Imported and FVB/NImported.
    Tissue: ColonImported and RetinaImported.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 391-2009 (ISOFORM 3).
    Tissue: BrainImported.
  5. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1261-1274, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  6. "The ciliary rootlet maintains long-term stability of sensory cilia."
    Yang J., Gao J., Adamian M., Wen X.-H., Pawlyk B., Zhang L., Sanderson M.J., Zuo J., Makino C.L., Li T.
    Mol. Cell. Biol. 25:4129-4137(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Rootletin interacts with C-Nap1 and may function as a physical linker between the pair of centrioles/basal bodies in cells."
    Yang J., Adamian M., Li T.
    Mol. Biol. Cell 17:1033-1040(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CEP250.

Entry informationi

Entry nameiCROCC_MOUSE
AccessioniPrimary (citable) accession number: Q8CJ40
Secondary accession number(s): A2AA81
, Q7TQL2, Q80U01, Q8R0B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3