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Q8CJ40

- CROCC_MOUSE

UniProt

Q8CJ40 - CROCC_MOUSE

Protein

Rootletin

Gene

Crocc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. Contributes to centrosome cohesion before mitosis.By similarity1 Publication

    GO - Molecular functioni

    1. kinesin binding Source: MGI
    2. structural molecule activity Source: UniProtKB

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell projection organization Source: UniProtKB-KW
    3. centrosome organization Source: UniProtKB
    4. protein localization Source: Ensembl

    Keywords - Biological processi

    Cell cycle, Cilium biogenesis/degradation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rootletin
    Alternative name(s):
    Ciliary rootlet coiled-coil protein
    Gene namesi
    Name:CroccImported
    Synonyms:Kiaa0445Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:3529431. Crocc.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole 2 Publications
    Note: In ciliated cells, associated with ciliary rootlets. In non-ciliated cells, localized between, around and at the proximal ends of the centrioles. Dissociates from the centrioles at the onset of mitosis and reassociates with them at anaphase.

    GO - Cellular componenti

    1. actin cytoskeleton Source: Ensembl
    2. centriole Source: UniProtKB
    3. ciliary rootlet Source: MGI
    4. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Disruption phenotypei

    Mice have no ciliary rootlets in ciliated cells.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 20092009RootletinPRO_0000239944Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1453 – 14531PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by NEK2 which may regulate its association with centrosomes.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8CJ40.
    PaxDbiQ8CJ40.
    PRIDEiQ8CJ40.

    PTM databases

    PhosphoSiteiQ8CJ40.

    Expressioni

    Tissue specificityi

    Highest expression detected in photoreceptor cells of retina. Expressed at lower levels in brain, trachea and kidney. Detected in all major ciliated epithelia. During embryonic development, enriched along the apical domains of neuroepithelium in brain ventricular zone, in primordia of retinal pigment epithelia and in neural retina.1 Publication

    Gene expression databases

    ArrayExpressiQ8CJ40.
    BgeeiQ8CJ40.
    CleanExiMM_CROCC.
    GenevestigatoriQ8CJ40.

    Interactioni

    Subunit structurei

    Homopolymer. Interacts with KLC3, NEK2 and the N-terminus of CEP250.2 Publications

    Protein-protein interaction databases

    BioGridi231046. 5 interactions.
    IntActiQ8CJ40. 1 interaction.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili74 – 265192Sequence AnalysisAdd
    BLAST
    Coiled coili346 – 43893Sequence AnalysisAdd
    BLAST
    Coiled coili550 – 1058509Sequence AnalysisAdd
    BLAST
    Coiled coili1091 – 1439349Sequence AnalysisAdd
    BLAST
    Coiled coili1498 – 1697200Sequence AnalysisAdd
    BLAST
    Coiled coili1744 – 1998255Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rootletin family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00730000110800.
    HOGENOMiHOG000231187.
    HOVERGENiHBG080162.
    InParanoidiA2AA81.
    KOiK16469.
    OMAiYKKRCSE.
    OrthoDBiEOG7W6WJX.
    TreeFamiTF101138.

    Family and domain databases

    InterProiIPR026733. Rootletin.
    [Graphical view]
    PANTHERiPTHR23159:SF11. PTHR23159:SF11. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: Q8CJ40-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLGLAGSLQ AQLALEIVIQ SLENCVLGPN QEKSLSVQNR VQDFQGASLL     50
    VCAREVIASN LSRPETPAPL QVPEMASLLS LQEENQLLQQ ELSRVEDLLA 100
    QSRAERDELA IKYNAVNERL EQAVRLETGE LEAQEPRGLV RQSVELRRQL 150
    QEEQSSYRRK LQAYQEGQQR QAQLVQRLQA KILQYKKQCS ELEKQLMDRS 200
    TELEQQRLRD TEHSQDLDSA LLRLEEEQQR SASLAQVNAM LREQLDQANL 250
    ANQALSEDIR KVTSDWTRSC KELEQREAVW RREEESFNTY FSSEHSRLLR 300
    LWRQVMGLRR QASEVKMGTE RDLLQLGGEL VRTSRAVQEL GLGLSASLHR 350
    AESKAEAALE KQKLLQAQLE EQLQAKLLRE KDLAQLQVQS DLDKADLSAR 400
    VTELALSVEH LQNQNSEKDQ VNRTLSDKLE ALESLRLQEQ TTLDTEDGEG 450
    LQQTLRDLAQ AALSDTESGV QLSSSERTAD TSDGSLRGFS GQRTPTPPRH 500
    SPGRGRSPRR GLSPACSDSS TLTLIHSALH KRQLQVQDMR GRYEASQELL 550
    GSVRKQLSDS EGERRGLEEQ LQRLRDQTAA SAQAQEDAQR EAQRLRSANE 600
    LLSREKGNLT HSLQVTQQQA KELRQELEKL QAAQEELKRQ HNQLEDAQED 650
    SVQEGARARR ELERSHRQLE QLEVKRSGLT KELVEVREAL SCAILQRDVL 700
    QTEKAEVAEA LTKAEAGRAQ LELSLTKLRA EEASLRDSLS KMSALNESLA 750
    QDKLELNRLI AQLEEEKVAL LGRQQQAEHA TTMAVEKQEL LEQLRLEQEV 800
    ERQGLQGSLC VAEQAREALE QQILVLRSER SHLQEQLAQL SRQLSGRDQE 850
    LEQALRESQR QVEALERAAR EKEAMAKERA GLAVKLAAAE REGRTLSEEA 900
    IRLRLEKEAL ESSLFDVQRQ LAQLEARREQ LEADSQALLL AKETLTGELA 950
    GLRQQVTSTE EKAALDKELM TQKLVQAERE AQASLREQRA AHEEDLQRLQ 1000
    HEKEAAWREL QAERAQLQGQ LQQEREELLA RMEAEKEELS KEIAALQQER 1050
    DEGLLLAESE KQQALSLKES EKTALSEKLM GTRHSLAAIS LEMERQKRDA 1100
    QSRQEQDRNT LNALTSELRD LRAQLEEATA AHAQTVKELE ERTGNLGRQR 1150
    EACMREAEEL RTQLRVLEDT RDGLRRELLE AQRKGRDSQD SSEAHRQEAS 1200
    ELRRSLSEGA KEREALRRSN EELRSAVKKA ESERISLKLA NEDKEQKLAL 1250
    LEEARVSVAK EAGELRASLQ EVERSRLEAR RELQELRRQM KTLDSDNGRL 1300
    GRELADLQGR LALGERTEKE SRREALGLRQ RLLKGESSLE ALKQELQGSQ 1350
    RKLQEQEAEF RARERGLLGS LEEARGAEKR LLDSARSLEL RLEAVRAETS 1400
    ELGLRLSAAE GRAQGLEVEL ARVEAQRRVA EAQLGGLRSA LRRGLGLGRV 1450
    SSSPAREAPA GGSGDGLSSP SPLEYSPRSQ PPSPGLIASP APPDLDPEAV 1500
    RDALRDFLQE LRSAQRERDE LKVQTSTLSQ QLVEMEAERD HAASRAKQLQ 1550
    KAVAESEEAW RSADRRLSGA QAELALQEES VRRSKRECRA TLDQMAVLER 1600
    SLQATESELR ASQEKVSKMK ATEAKLESDK RRLKEVLDAS ESRSIKLELQ 1650
    RRALEGELQR SRLGLGDREA HAQALQDRVD SLQRQVADSE VKAGTLQLTV 1700
    ERLSGALAKV EESEGNLRSK VQSLTDALTQ SSASLSSTQD KNLHLQKALS 1750
    TCEHDRQVLQ ERLDAARQAL SEARRQSSSL GEQVQTLRGE LASLELQRGD 1800
    AEGQLQQLQQ ALRQRQEGEA MALRSVQKLQ EERRLLQERL GSLQRALAQL 1850
    EAEKRDLERS ALQFDKDRVA LRKTLDKVER EKLRSHEDTL RLNAERGRLD 1900
    RTLTGAELDL AEAQQQIQHL EAQVDVALEG NHNPVQPEAG EQQLELQQEV 1950
    ERLRSAQVQT ERTLEARERA HRQRVSGLEE QVSTLKAQLH QELRRSSASV 2000
    SLPPGTPEK 2009
    Length:2,009
    Mass (Da):226,945
    Last modified:July 27, 2011 - v2
    Checksum:i62D942401C1085DA
    GO
    Isoform 21 Publication (identifier: Q8CJ40-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-74: Missing.
         120-209: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:1,845
    Mass (Da):208,269
    Checksum:i12C8A15759C8B64D
    GO
    Isoform 31 Publication (identifier: Q8CJ40-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1136-1156: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:1,988
    Mass (Da):224,458
    Checksum:i0F53D354E8559A2B
    GO

    Sequence cautioni

    The sequence AAH27090.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti289 – 2891T → A in AAN73044. (PubMed:12427867)Curated
    Sequence conflicti289 – 2891T → A in AAH54054. (PubMed:15489334)Curated
    Sequence conflicti360 – 3601E → G in AAH54054. (PubMed:15489334)Curated
    Sequence conflicti811 – 8111V → M in AAH54054. (PubMed:15489334)Curated
    Sequence conflicti1041 – 10411K → E in AAH54054. (PubMed:15489334)Curated
    Sequence conflicti1346 – 13461L → V in BAC65567. (PubMed:15489334)Curated
    Sequence conflicti1864 – 18641F → L in AAH54054. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7474Missing in isoform 2. 1 PublicationVSP_052066Add
    BLAST
    Alternative sequencei120 – 20990Missing in isoform 2. 1 PublicationVSP_052067Add
    BLAST
    Alternative sequencei1136 – 115621Missing in isoform 3. 1 PublicationVSP_052068Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF527975 mRNA. Translation: AAN73044.1.
    AL645625 Genomic DNA. Translation: CAM16757.1.
    BC027090 mRNA. Translation: AAH27090.1. Different initiation.
    BC054054 mRNA. Translation: AAH54054.1.
    AK122285 Transcribed RNA. Translation: BAC65567.3.
    CCDSiCCDS18861.1. [Q8CJ40-1]
    CCDS51346.1. [Q8CJ40-2]
    RefSeqiNP_001139430.1. NM_001145958.1. [Q8CJ40-2]
    NP_742120.2. NM_172122.2. [Q8CJ40-1]
    UniGeneiMm.80685.

    Genome annotation databases

    EnsembliENSMUST00000040222; ENSMUSP00000037679; ENSMUSG00000040860. [Q8CJ40-2]
    ENSMUST00000097816; ENSMUSP00000095425; ENSMUSG00000040860. [Q8CJ40-2]
    ENSMUST00000102491; ENSMUSP00000099549; ENSMUSG00000040860. [Q8CJ40-1]
    ENSMUST00000168157; ENSMUSP00000126543; ENSMUSG00000040860. [Q8CJ40-2]
    GeneIDi230872.
    KEGGimmu:230872.
    UCSCiuc008vns.2. mouse. [Q8CJ40-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF527975 mRNA. Translation: AAN73044.1 .
    AL645625 Genomic DNA. Translation: CAM16757.1 .
    BC027090 mRNA. Translation: AAH27090.1 . Different initiation.
    BC054054 mRNA. Translation: AAH54054.1 .
    AK122285 Transcribed RNA. Translation: BAC65567.3 .
    CCDSi CCDS18861.1. [Q8CJ40-1 ]
    CCDS51346.1. [Q8CJ40-2 ]
    RefSeqi NP_001139430.1. NM_001145958.1. [Q8CJ40-2 ]
    NP_742120.2. NM_172122.2. [Q8CJ40-1 ]
    UniGenei Mm.80685.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 231046. 5 interactions.
    IntActi Q8CJ40. 1 interaction.

    PTM databases

    PhosphoSitei Q8CJ40.

    Proteomic databases

    MaxQBi Q8CJ40.
    PaxDbi Q8CJ40.
    PRIDEi Q8CJ40.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000040222 ; ENSMUSP00000037679 ; ENSMUSG00000040860 . [Q8CJ40-2 ]
    ENSMUST00000097816 ; ENSMUSP00000095425 ; ENSMUSG00000040860 . [Q8CJ40-2 ]
    ENSMUST00000102491 ; ENSMUSP00000099549 ; ENSMUSG00000040860 . [Q8CJ40-1 ]
    ENSMUST00000168157 ; ENSMUSP00000126543 ; ENSMUSG00000040860 . [Q8CJ40-2 ]
    GeneIDi 230872.
    KEGGi mmu:230872.
    UCSCi uc008vns.2. mouse. [Q8CJ40-1 ]

    Organism-specific databases

    CTDi 9696.
    MGIi MGI:3529431. Crocc.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00730000110800.
    HOGENOMi HOG000231187.
    HOVERGENi HBG080162.
    InParanoidi A2AA81.
    KOi K16469.
    OMAi YKKRCSE.
    OrthoDBi EOG7W6WJX.
    TreeFami TF101138.

    Miscellaneous databases

    ChiTaRSi CROCC. mouse.
    NextBioi 380242.
    PROi Q8CJ40.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CJ40.
    Bgeei Q8CJ40.
    CleanExi MM_CROCC.
    Genevestigatori Q8CJ40.

    Family and domain databases

    InterProi IPR026733. Rootletin.
    [Graphical view ]
    PANTHERi PTHR23159:SF11. PTHR23159:SF11. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Rootletin, a novel coiled-coil protein, is a structural component of the ciliary rootlet."
      Yang J., Liu X., Yue G., Adamian M., Bulgakov O., Li T.
      J. Cell Biol. 159:431-440(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH KLC3.
      Strain: C57BL/6Imported.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6Imported and FVB/NImported.
      Tissue: ColonImported and RetinaImported.
    4. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 391-2009 (ISOFORM 3).
      Tissue: BrainImported.
    5. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 1261-1274, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    6. "The ciliary rootlet maintains long-term stability of sensory cilia."
      Yang J., Gao J., Adamian M., Wen X.-H., Pawlyk B., Zhang L., Sanderson M.J., Zuo J., Makino C.L., Li T.
      Mol. Cell. Biol. 25:4129-4137(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    7. "Rootletin interacts with C-Nap1 and may function as a physical linker between the pair of centrioles/basal bodies in cells."
      Yang J., Adamian M., Li T.
      Mol. Biol. Cell 17:1033-1040(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CEP250.

    Entry informationi

    Entry nameiCROCC_MOUSE
    AccessioniPrimary (citable) accession number: Q8CJ40
    Secondary accession number(s): A2AA81
    , Q7TQL2, Q80U01, Q8R0B9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3