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Protein

Rootletin

Gene

Crocc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. Contributes to centrosome cohesion before mitosis.By similarity1 Publication

GO - Molecular functioni

  • actin binding Source: MGI
  • kinesin binding Source: MGI
  • structural molecule activity Source: UniProtKB

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • centriole-centriole cohesion Source: MGI
  • centrosome organization Source: UniProtKB
  • ciliary basal body organization Source: MGI
  • epithelial structure maintenance Source: MGI
  • establishment of organelle localization Source: MGI
  • photoreceptor cell maintenance Source: MGI
  • protein localization Source: MGI
  • protein localization to organelle Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Rootletin
Alternative name(s):
Ciliary rootlet coiled-coil protein
Gene namesi
Name:CroccImported
Synonyms:Kiaa0445Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:3529431. Crocc.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • centriole Source: UniProtKB
  • centrosome Source: MGI
  • ciliary rootlet Source: MGI
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • photoreceptor inner segment Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Mice have no ciliary rootlets in ciliated cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002399441 – 2009RootletinAdd BLAST2009

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1453PhosphoserineBy similarity1
Modified residuei1463PhosphoserineCombined sources1
Modified residuei1469PhosphoserineCombined sources1
Modified residuei1475PhosphotyrosineCombined sources1
Modified residuei1476PhosphoserineCombined sources1
Modified residuei1479PhosphoserineCombined sources1
Modified residuei1483PhosphoserineCombined sources1
Modified residuei1489PhosphoserineCombined sources1
Modified residuei1568PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by NEK2 which may regulate its association with centrosomes.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CJ40.
PeptideAtlasiQ8CJ40.
PRIDEiQ8CJ40.

PTM databases

iPTMnetiQ8CJ40.
PhosphoSitePlusiQ8CJ40.

Expressioni

Tissue specificityi

Highest expression detected in photoreceptor cells of retina. Expressed at lower levels in brain, trachea and kidney. Detected in all major ciliated epithelia. During embryonic development, enriched along the apical domains of neuroepithelium in brain ventricular zone, in primordia of retinal pigment epithelia and in neural retina.1 Publication

Gene expression databases

BgeeiENSMUSG00000040860.
CleanExiMM_CROCC.
ExpressionAtlasiQ8CJ40. baseline and differential.
GenevisibleiQ8CJ40. MM.

Interactioni

Subunit structurei

Homopolymer. Interacts with KLC3, NEK2 and the N-terminus of CEP250.2 Publications

GO - Molecular functioni

  • actin binding Source: MGI
  • kinesin binding Source: MGI

Protein-protein interaction databases

BioGridi231046. 9 interactors.
IntActiQ8CJ40. 5 interactors.
STRINGi10090.ENSMUSP00000099549.

Structurei

3D structure databases

ProteinModelPortaliQ8CJ40.
SMRiQ8CJ40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili74 – 265Sequence analysisAdd BLAST192
Coiled coili346 – 438Sequence analysisAdd BLAST93
Coiled coili550 – 1058Sequence analysisAdd BLAST509
Coiled coili1091 – 1439Sequence analysisAdd BLAST349
Coiled coili1498 – 1697Sequence analysisAdd BLAST200
Coiled coili1744 – 1998Sequence analysisAdd BLAST255

Sequence similaritiesi

Belongs to the rootletin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFAN. Eukaryota.
ENOG410XPG3. LUCA.
GeneTreeiENSGT00730000110800.
HOGENOMiHOG000231187.
HOVERGENiHBG080162.
InParanoidiQ8CJ40.
KOiK16469.
OMAiSDWTRSC.
OrthoDBiEOG091G034H.
TreeFamiTF101138.

Family and domain databases

InterProiIPR026733. Rootletin.
[Graphical view]
PANTHERiPTHR23159:SF17. PTHR23159:SF17. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q8CJ40-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLGLAGSLQ AQLALEIVIQ SLENCVLGPN QEKSLSVQNR VQDFQGASLL
60 70 80 90 100
VCAREVIASN LSRPETPAPL QVPEMASLLS LQEENQLLQQ ELSRVEDLLA
110 120 130 140 150
QSRAERDELA IKYNAVNERL EQAVRLETGE LEAQEPRGLV RQSVELRRQL
160 170 180 190 200
QEEQSSYRRK LQAYQEGQQR QAQLVQRLQA KILQYKKQCS ELEKQLMDRS
210 220 230 240 250
TELEQQRLRD TEHSQDLDSA LLRLEEEQQR SASLAQVNAM LREQLDQANL
260 270 280 290 300
ANQALSEDIR KVTSDWTRSC KELEQREAVW RREEESFNTY FSSEHSRLLR
310 320 330 340 350
LWRQVMGLRR QASEVKMGTE RDLLQLGGEL VRTSRAVQEL GLGLSASLHR
360 370 380 390 400
AESKAEAALE KQKLLQAQLE EQLQAKLLRE KDLAQLQVQS DLDKADLSAR
410 420 430 440 450
VTELALSVEH LQNQNSEKDQ VNRTLSDKLE ALESLRLQEQ TTLDTEDGEG
460 470 480 490 500
LQQTLRDLAQ AALSDTESGV QLSSSERTAD TSDGSLRGFS GQRTPTPPRH
510 520 530 540 550
SPGRGRSPRR GLSPACSDSS TLTLIHSALH KRQLQVQDMR GRYEASQELL
560 570 580 590 600
GSVRKQLSDS EGERRGLEEQ LQRLRDQTAA SAQAQEDAQR EAQRLRSANE
610 620 630 640 650
LLSREKGNLT HSLQVTQQQA KELRQELEKL QAAQEELKRQ HNQLEDAQED
660 670 680 690 700
SVQEGARARR ELERSHRQLE QLEVKRSGLT KELVEVREAL SCAILQRDVL
710 720 730 740 750
QTEKAEVAEA LTKAEAGRAQ LELSLTKLRA EEASLRDSLS KMSALNESLA
760 770 780 790 800
QDKLELNRLI AQLEEEKVAL LGRQQQAEHA TTMAVEKQEL LEQLRLEQEV
810 820 830 840 850
ERQGLQGSLC VAEQAREALE QQILVLRSER SHLQEQLAQL SRQLSGRDQE
860 870 880 890 900
LEQALRESQR QVEALERAAR EKEAMAKERA GLAVKLAAAE REGRTLSEEA
910 920 930 940 950
IRLRLEKEAL ESSLFDVQRQ LAQLEARREQ LEADSQALLL AKETLTGELA
960 970 980 990 1000
GLRQQVTSTE EKAALDKELM TQKLVQAERE AQASLREQRA AHEEDLQRLQ
1010 1020 1030 1040 1050
HEKEAAWREL QAERAQLQGQ LQQEREELLA RMEAEKEELS KEIAALQQER
1060 1070 1080 1090 1100
DEGLLLAESE KQQALSLKES EKTALSEKLM GTRHSLAAIS LEMERQKRDA
1110 1120 1130 1140 1150
QSRQEQDRNT LNALTSELRD LRAQLEEATA AHAQTVKELE ERTGNLGRQR
1160 1170 1180 1190 1200
EACMREAEEL RTQLRVLEDT RDGLRRELLE AQRKGRDSQD SSEAHRQEAS
1210 1220 1230 1240 1250
ELRRSLSEGA KEREALRRSN EELRSAVKKA ESERISLKLA NEDKEQKLAL
1260 1270 1280 1290 1300
LEEARVSVAK EAGELRASLQ EVERSRLEAR RELQELRRQM KTLDSDNGRL
1310 1320 1330 1340 1350
GRELADLQGR LALGERTEKE SRREALGLRQ RLLKGESSLE ALKQELQGSQ
1360 1370 1380 1390 1400
RKLQEQEAEF RARERGLLGS LEEARGAEKR LLDSARSLEL RLEAVRAETS
1410 1420 1430 1440 1450
ELGLRLSAAE GRAQGLEVEL ARVEAQRRVA EAQLGGLRSA LRRGLGLGRV
1460 1470 1480 1490 1500
SSSPAREAPA GGSGDGLSSP SPLEYSPRSQ PPSPGLIASP APPDLDPEAV
1510 1520 1530 1540 1550
RDALRDFLQE LRSAQRERDE LKVQTSTLSQ QLVEMEAERD HAASRAKQLQ
1560 1570 1580 1590 1600
KAVAESEEAW RSADRRLSGA QAELALQEES VRRSKRECRA TLDQMAVLER
1610 1620 1630 1640 1650
SLQATESELR ASQEKVSKMK ATEAKLESDK RRLKEVLDAS ESRSIKLELQ
1660 1670 1680 1690 1700
RRALEGELQR SRLGLGDREA HAQALQDRVD SLQRQVADSE VKAGTLQLTV
1710 1720 1730 1740 1750
ERLSGALAKV EESEGNLRSK VQSLTDALTQ SSASLSSTQD KNLHLQKALS
1760 1770 1780 1790 1800
TCEHDRQVLQ ERLDAARQAL SEARRQSSSL GEQVQTLRGE LASLELQRGD
1810 1820 1830 1840 1850
AEGQLQQLQQ ALRQRQEGEA MALRSVQKLQ EERRLLQERL GSLQRALAQL
1860 1870 1880 1890 1900
EAEKRDLERS ALQFDKDRVA LRKTLDKVER EKLRSHEDTL RLNAERGRLD
1910 1920 1930 1940 1950
RTLTGAELDL AEAQQQIQHL EAQVDVALEG NHNPVQPEAG EQQLELQQEV
1960 1970 1980 1990 2000
ERLRSAQVQT ERTLEARERA HRQRVSGLEE QVSTLKAQLH QELRRSSASV

SLPPGTPEK
Length:2,009
Mass (Da):226,945
Last modified:July 27, 2011 - v2
Checksum:i62D942401C1085DA
GO
Isoform 21 Publication (identifier: Q8CJ40-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.
     120-209: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:1,845
Mass (Da):208,269
Checksum:i12C8A15759C8B64D
GO
Isoform 31 Publication (identifier: Q8CJ40-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1136-1156: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:1,988
Mass (Da):224,458
Checksum:i0F53D354E8559A2B
GO

Sequence cautioni

The sequence AAH27090 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti289T → A in AAN73044 (PubMed:12427867).Curated1
Sequence conflicti289T → A in AAH54054 (PubMed:15489334).Curated1
Sequence conflicti360E → G in AAH54054 (PubMed:15489334).Curated1
Sequence conflicti811V → M in AAH54054 (PubMed:15489334).Curated1
Sequence conflicti1041K → E in AAH54054 (PubMed:15489334).Curated1
Sequence conflicti1346L → V in BAC65567 (PubMed:15489334).Curated1
Sequence conflicti1864F → L in AAH54054 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0520661 – 74Missing in isoform 2. 1 PublicationAdd BLAST74
Alternative sequenceiVSP_052067120 – 209Missing in isoform 2. 1 PublicationAdd BLAST90
Alternative sequenceiVSP_0520681136 – 1156Missing in isoform 3. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF527975 mRNA. Translation: AAN73044.1.
AL645625 Genomic DNA. Translation: CAM16757.1.
BC027090 mRNA. Translation: AAH27090.1. Different initiation.
BC054054 mRNA. Translation: AAH54054.1.
AK122285 Transcribed RNA. Translation: BAC65567.3.
CCDSiCCDS18861.1. [Q8CJ40-1]
CCDS51346.1. [Q8CJ40-2]
RefSeqiNP_001139430.1. NM_001145958.1. [Q8CJ40-2]
NP_742120.2. NM_172122.2. [Q8CJ40-1]
UniGeneiMm.80685.

Genome annotation databases

EnsembliENSMUST00000040222; ENSMUSP00000037679; ENSMUSG00000040860. [Q8CJ40-2]
ENSMUST00000097816; ENSMUSP00000095425; ENSMUSG00000040860. [Q8CJ40-2]
ENSMUST00000102491; ENSMUSP00000099549; ENSMUSG00000040860. [Q8CJ40-1]
ENSMUST00000168157; ENSMUSP00000126543; ENSMUSG00000040860. [Q8CJ40-2]
GeneIDi230872.
KEGGimmu:230872.
UCSCiuc008vns.2. mouse. [Q8CJ40-1]
uc012dnz.1. mouse. [Q8CJ40-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF527975 mRNA. Translation: AAN73044.1.
AL645625 Genomic DNA. Translation: CAM16757.1.
BC027090 mRNA. Translation: AAH27090.1. Different initiation.
BC054054 mRNA. Translation: AAH54054.1.
AK122285 Transcribed RNA. Translation: BAC65567.3.
CCDSiCCDS18861.1. [Q8CJ40-1]
CCDS51346.1. [Q8CJ40-2]
RefSeqiNP_001139430.1. NM_001145958.1. [Q8CJ40-2]
NP_742120.2. NM_172122.2. [Q8CJ40-1]
UniGeneiMm.80685.

3D structure databases

ProteinModelPortaliQ8CJ40.
SMRiQ8CJ40.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231046. 9 interactors.
IntActiQ8CJ40. 5 interactors.
STRINGi10090.ENSMUSP00000099549.

PTM databases

iPTMnetiQ8CJ40.
PhosphoSitePlusiQ8CJ40.

Proteomic databases

PaxDbiQ8CJ40.
PeptideAtlasiQ8CJ40.
PRIDEiQ8CJ40.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040222; ENSMUSP00000037679; ENSMUSG00000040860. [Q8CJ40-2]
ENSMUST00000097816; ENSMUSP00000095425; ENSMUSG00000040860. [Q8CJ40-2]
ENSMUST00000102491; ENSMUSP00000099549; ENSMUSG00000040860. [Q8CJ40-1]
ENSMUST00000168157; ENSMUSP00000126543; ENSMUSG00000040860. [Q8CJ40-2]
GeneIDi230872.
KEGGimmu:230872.
UCSCiuc008vns.2. mouse. [Q8CJ40-1]
uc012dnz.1. mouse. [Q8CJ40-2]

Organism-specific databases

CTDi9696.
MGIiMGI:3529431. Crocc.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IFAN. Eukaryota.
ENOG410XPG3. LUCA.
GeneTreeiENSGT00730000110800.
HOGENOMiHOG000231187.
HOVERGENiHBG080162.
InParanoidiQ8CJ40.
KOiK16469.
OMAiSDWTRSC.
OrthoDBiEOG091G034H.
TreeFamiTF101138.

Miscellaneous databases

ChiTaRSiCrocc. mouse.
PROiQ8CJ40.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040860.
CleanExiMM_CROCC.
ExpressionAtlasiQ8CJ40. baseline and differential.
GenevisibleiQ8CJ40. MM.

Family and domain databases

InterProiIPR026733. Rootletin.
[Graphical view]
PANTHERiPTHR23159:SF17. PTHR23159:SF17. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCROCC_MOUSE
AccessioniPrimary (citable) accession number: Q8CJ40
Secondary accession number(s): A2AA81
, Q7TQL2, Q80U01, Q8R0B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.