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Protein

Adhesion G-protein coupled receptor G2

Gene

Adgrg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Orphan receptor. Could be involved in a signal transduction pathway controlling epididymal function and male fertility. May regulate fluid exchange within epididymis.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Protein family/group databases

MEROPSiP02.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G-protein coupled receptor G2
Alternative name(s):
G-protein coupled receptor 64
Mouse epididymis-specific protein 6
Short name:
Me6
Gene namesi
Name:Adgrg2Imported
Synonyms:Gpr64, Me6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2446854. Adgrg2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini38 – 619ExtracellularCuratedAdd BLAST582
Transmembranei620 – 640Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini641 – 659CytoplasmicCuratedAdd BLAST19
Transmembranei660 – 680Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini681 – 683ExtracellularCurated3
Transmembranei684 – 704Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini705 – 729CytoplasmicCuratedAdd BLAST25
Transmembranei730 – 750Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini751 – 781ExtracellularCuratedAdd BLAST31
Transmembranei782 – 802Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini803 – 826CytoplasmicCuratedAdd BLAST24
Transmembranei827 – 847Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini848 – 849ExtracellularCurated2
Transmembranei850 – 870Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini871 – 1009CytoplasmicCuratedAdd BLAST139

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant male are infertile. Targeted disruption leads to sperm stasis and duct obstruction, resulting from dysregulation of fluid reabsorption.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Sequence analysisAdd BLAST37
ChainiPRO_000001288738 – 1009Adhesion G-protein coupled receptor G2Add BLAST972

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi43N-linked (GlcNAc...)Sequence analysis1
Glycosylationi77N-linked (GlcNAc...)Sequence analysis1
Glycosylationi91N-linked (GlcNAc...)Sequence analysis1
Glycosylationi103N-linked (GlcNAc...)Sequence analysis1
Glycosylationi109N-linked (GlcNAc...)Sequence analysis1
Glycosylationi127N-linked (GlcNAc...)Sequence analysis1
Glycosylationi136N-linked (GlcNAc...)Sequence analysis1
Glycosylationi154N-linked (GlcNAc...)Sequence analysis1
Glycosylationi178N-linked (GlcNAc...)Sequence analysis1
Glycosylationi186N-linked (GlcNAc...)Sequence analysis1
Glycosylationi362N-linked (GlcNAc...)Sequence analysis1
Glycosylationi427N-linked (GlcNAc...)Sequence analysis1
Glycosylationi448N-linked (GlcNAc...)Sequence analysis1
Glycosylationi453N-linked (GlcNAc...)Sequence analysis1
Glycosylationi520N-linked (GlcNAc...)Sequence analysis1
Glycosylationi534N-linked (GlcNAc...)Sequence analysis1
Glycosylationi539N-linked (GlcNAc...)Sequence analysis1
Glycosylationi543N-linked (GlcNAc...)Sequence analysis1
Glycosylationi589N-linked (GlcNAc...)Sequence analysis1
Glycosylationi849N-linked (GlcNAc...)Sequence analysis1
Modified residuei1002PhosphoserineCombined sources1

Post-translational modificationi

Proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit.Curated
Highly glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8CJ12.
PRIDEiQ8CJ12.

PTM databases

iPTMnetiQ8CJ12.
PhosphoSitePlusiQ8CJ12.

Expressioni

Tissue specificityi

Epididymis-specific expression (at protein level). Associated with apical membranes of efferent ductule and proximal epididymal duct epithelia. Mainly expressed in the nonciliated principal cells of the proximal excurrent ducts.2 Publications

Gene expression databases

BgeeiENSMUSG00000031298.
CleanExiMM_GPR64.
ExpressionAtlasiQ8CJ12. baseline and differential.
GenevisibleiQ8CJ12. MM.

Interactioni

Subunit structurei

Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked.Curated

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000108019.

Structurei

3D structure databases

ProteinModelPortaliQ8CJ12.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini559 – 610GPSPROSITE-ProRule annotationAdd BLAST52

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi248 – 251Poly-Ser4
Compositional biasi664 – 669Poly-Leu6
Compositional biasi808 – 811Poly-Lys4
Compositional biasi917 – 922Poly-Ser6

Sequence similaritiesi

Contains 1 GPS domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOVERGENiHBG051817.
InParanoidiQ8CJ12.
KOiK08451.
OMAiFIFYCVA.
OrthoDBiEOG091G00P5.
PhylomeDBiQ8CJ12.
TreeFamiTF321769.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
SMARTiSM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CJ12-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLFSGGQYSP VGRPEEVLLI YKIFLVIICF HVILVTSLKE NGNSSLLSPS
60 70 80 90 100
AESSLVSLIP YSNGTPDAAS EVLSTLNKTE KSKITIVKTF NASGVKSQRN
110 120 130 140 150
ICNLSSLCND SVFFRGEIVF QHDEDHNVTQ NQDTANGTFA GVLSLSELKR
160 170 180 190 200
SELNKTLQTL SETYFIVCAT AEAQSTVNCT FTVKLNETMN VCAMMVTFQT
210 220 230 240 250
VQIRPMEQCC CSPRTPCPSS PEELEKLQCE LQDPIVCLAD QPHGPPLSSS
260 270 280 290 300
SKPVVPQATI ISHVASDFSL AEPLDHALMT PSTPSLTQES NLPSPQPTIP
310 320 330 340 350
LASSPATDLP VQSVVVSSLP QTDLSHTLSP VQSSIPSPTT PAPSVPTELV
360 370 380 390 400
TISTPPGETV VNTSTVSDLE AQVSQMEKAL SLGSLEPNLA GEMVNRVSKL
410 420 430 440 450
LHSPPALLAP LAQRLLKVVD AIGLQLNFSS TTISLTSPSL ALAVIRVNAS
460 470 480 490 500
NFNTTTFAAQ DPTNLQVSLE TPPPENSIGA ITLPSSLMNN LPANDVELAS
510 520 530 540 550
RIQFNFFETP ALFQDPSLEN LTLISYVISS SVTNMTIKNL TRNVTVALKH
560 570 580 590 600
INPSPDDLTV KCVFWDLGRN GGKGGWSSDG CSVKDKRMNE TICTCSHLTS
610 620 630 640 650
FGILLDLSRT SLPPSQMMAL TFITYIGCGL SSIFLSVTLV TYIAFEKIRR
660 670 680 690 700
DYPSKILIQL CAALLLLNLI FLLDSWIALY NTRGFCIAVA VFLHYFLLVS
710 720 730 740 750
FTWMGLEAFH MYLALVKVFN TYIRKYILKF CIVGWGIPAV VVSIVLTISP
760 770 780 790 800
DNYGIGSYGK FPNGTPDDFC WINSNVVFYI TVVGYFCVIF LLNVSMFIVV
810 820 830 840 850
LVQLCRIKKK KQLGAQRKTS IQDLRSIAGL TFLLGITWGF AFFAWGPVNV
860 870 880 890 900
TFMYLFAIFN TLQGFFIFIF YCAAKENVRK QWRRYLCCGK LRLAENSDWS
910 920 930 940 950
KTATNGLKKQ TVNQGVSSSS NSLQSSCNST NSTTLLVNSD CSVHASGNGN
960 970 980 990 1000
ASTERNGVSF SVQNGDVCLH DLTGKQHMFS DKEDSCNGKS RIALRRTSKR

GSLHFIEQM
Length:1,009
Mass (Da):110,200
Last modified:March 1, 2003 - v1
Checksum:iA53C67C5527A5B6C
GO
Isoform 2 (identifier: Q8CJ12-2) [UniParc]FASTAAdd to basket
Also known as: d2

The sequence of this isoform differs from the canonical sequence as follows:
     64-66: Missing.

Show »
Length:1,006
Mass (Da):109,945
Checksum:i84EB92A305DAAF64
GO
Isoform 3 (identifier: Q8CJ12-3) [UniParc]FASTAAdd to basket
Also known as: d1

The sequence of this isoform differs from the canonical sequence as follows:
     80-93: Missing.

Show »
Length:995
Mass (Da):108,652
Checksum:i0B11972D6B6DD226
GO
Isoform 4 (identifier: Q8CJ12-4) [UniParc]FASTAAdd to basket
Also known as: d3

The sequence of this isoform differs from the canonical sequence as follows:
     51-66: Missing.

Show »
Length:993
Mass (Da):108,583
Checksum:i4E63FEF1D355E6EF
GO
Isoform 5 (identifier: Q8CJ12-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     40-66: Missing.

Show »
Length:982
Mass (Da):107,497
Checksum:iA73D80E41A0E08B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti781T → K in BAC32902 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00980640 – 66Missing in isoform 5. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_00980751 – 66Missing in isoform 4. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_00980864 – 66Missing in isoform 2. 1 Publication3
Alternative sequenceiVSP_00980980 – 93Missing in isoform 3. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF538952 mRNA. Translation: AAN33054.1.
AF538955 mRNA. Translation: AAN33057.1.
AF538956 mRNA. Translation: AAN33058.1.
AF538957 mRNA. Translation: AAN33059.1.
AK046871 mRNA. Translation: BAC32902.1.
AL731801 Genomic DNA. Translation: CAM16675.1.
AL731801 Genomic DNA. Translation: CAM16676.1.
AL731801 Genomic DNA. Translation: CAM16677.1.
AL731801 Genomic DNA. Translation: CAM16680.1.
CCDSiCCDS41196.1. [Q8CJ12-1]
CCDS41197.1. [Q8CJ12-3]
CCDS41199.1. [Q8CJ12-5]
CCDS72459.1. [Q8CJ12-2]
CCDS81192.1. [Q8CJ12-4]
RefSeqiNP_001073317.1. NM_001079848.2. [Q8CJ12-5]
NP_001073326.1. NM_001079857.2. [Q8CJ12-3]
NP_001277374.1. NM_001290445.1. [Q8CJ12-4]
NP_001277375.1. NM_001290446.1. [Q8CJ12-2]
NP_848827.1. NM_178712.4. [Q8CJ12-1]
UniGeneiMm.213016.

Genome annotation databases

EnsembliENSMUST00000112400; ENSMUSP00000108019; ENSMUSG00000031298. [Q8CJ12-1]
ENSMUST00000112402; ENSMUSP00000108021; ENSMUSG00000031298. [Q8CJ12-3]
ENSMUST00000112404; ENSMUSP00000108023; ENSMUSG00000031298. [Q8CJ12-5]
ENSMUST00000112405; ENSMUSP00000108024; ENSMUSG00000031298. [Q8CJ12-4]
ENSMUST00000112408; ENSMUSP00000108027; ENSMUSG00000031298. [Q8CJ12-2]
GeneIDi237175.
KEGGimmu:237175.
UCSCiuc009utd.2. mouse. [Q8CJ12-1]
uc009ute.2. mouse. [Q8CJ12-3]
uc009utg.2. mouse. [Q8CJ12-5]
uc009uth.2. mouse. [Q8CJ12-2]
uc009uti.2. mouse. [Q8CJ12-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF538952 mRNA. Translation: AAN33054.1.
AF538955 mRNA. Translation: AAN33057.1.
AF538956 mRNA. Translation: AAN33058.1.
AF538957 mRNA. Translation: AAN33059.1.
AK046871 mRNA. Translation: BAC32902.1.
AL731801 Genomic DNA. Translation: CAM16675.1.
AL731801 Genomic DNA. Translation: CAM16676.1.
AL731801 Genomic DNA. Translation: CAM16677.1.
AL731801 Genomic DNA. Translation: CAM16680.1.
CCDSiCCDS41196.1. [Q8CJ12-1]
CCDS41197.1. [Q8CJ12-3]
CCDS41199.1. [Q8CJ12-5]
CCDS72459.1. [Q8CJ12-2]
CCDS81192.1. [Q8CJ12-4]
RefSeqiNP_001073317.1. NM_001079848.2. [Q8CJ12-5]
NP_001073326.1. NM_001079857.2. [Q8CJ12-3]
NP_001277374.1. NM_001290445.1. [Q8CJ12-4]
NP_001277375.1. NM_001290446.1. [Q8CJ12-2]
NP_848827.1. NM_178712.4. [Q8CJ12-1]
UniGeneiMm.213016.

3D structure databases

ProteinModelPortaliQ8CJ12.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000108019.

Protein family/group databases

MEROPSiP02.007.
GPCRDBiSearch...

PTM databases

iPTMnetiQ8CJ12.
PhosphoSitePlusiQ8CJ12.

Proteomic databases

PaxDbiQ8CJ12.
PRIDEiQ8CJ12.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112400; ENSMUSP00000108019; ENSMUSG00000031298. [Q8CJ12-1]
ENSMUST00000112402; ENSMUSP00000108021; ENSMUSG00000031298. [Q8CJ12-3]
ENSMUST00000112404; ENSMUSP00000108023; ENSMUSG00000031298. [Q8CJ12-5]
ENSMUST00000112405; ENSMUSP00000108024; ENSMUSG00000031298. [Q8CJ12-4]
ENSMUST00000112408; ENSMUSP00000108027; ENSMUSG00000031298. [Q8CJ12-2]
GeneIDi237175.
KEGGimmu:237175.
UCSCiuc009utd.2. mouse. [Q8CJ12-1]
uc009ute.2. mouse. [Q8CJ12-3]
uc009utg.2. mouse. [Q8CJ12-5]
uc009uth.2. mouse. [Q8CJ12-2]
uc009uti.2. mouse. [Q8CJ12-4]

Organism-specific databases

CTDi10149.
MGIiMGI:2446854. Adgrg2.

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOVERGENiHBG051817.
InParanoidiQ8CJ12.
KOiK08451.
OMAiFIFYCVA.
OrthoDBiEOG091G00P5.
PhylomeDBiQ8CJ12.
TreeFamiTF321769.

Miscellaneous databases

PROiQ8CJ12.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031298.
CleanExiMM_GPR64.
ExpressionAtlasiQ8CJ12. baseline and differential.
GenevisibleiQ8CJ12. MM.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
SMARTiSM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGRG2_MOUSE
AccessioniPrimary (citable) accession number: Q8CJ12
Secondary accession number(s): A2AHP8
, A2AHP9, A2AHQ0, A2AHQ3, Q8BL10, Q8CJ08, Q8CJ09, Q8CJ10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.