Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADPH oxidase activator 1

Gene

Noxa1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions as an activator of NOX1, a superoxide-producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-5668599. RHO GTPases Activate NADPH Oxidases.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH oxidase activator 1
Gene namesi
Name:Noxa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2449980. Noxa1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444NADPH oxidase activator 1PRO_0000314610Add
BLAST

Proteomic databases

PaxDbiQ8CJ00.
PRIDEiQ8CJ00.

PTM databases

iPTMnetiQ8CJ00.
PhosphoSiteiQ8CJ00.

Expressioni

Tissue specificityi

Widely expressed with a tissue distribution similar to the one of NOX1. Detected in colon, uterus, prostate, small intestine, stomach, lung, thyroid, aorta, inner ear and salivary glands. Expressed in colon, small intestine and aortic smooth muscle cells (at protein level).4 Publications

Gene expression databases

BgeeiQ8CJ00.
CleanExiMM_NOXA1.

Interactioni

Subunit structurei

NOX1, NOXA1, NOXO1, RAC1 and CYBA forms a functional multimeric complex supporting ROS production. Interaction with YWHAZ prevents the interaction of NOXA1 with NOXO1 and RAC1 and its targeting to membranes, hence reducing its ability to activate NOX1. Interacts (via N-terminus) with SH3PXD2A and SH3PXD2B; the interaction is direct (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037423.

Structurei

3D structure databases

ProteinModelPortaliQ8CJ00.
SMRiQ8CJ00. Positions 3-203, 373-423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati7 – 3832TPR 1Add
BLAST
Repeati39 – 7133TPR 2Add
BLAST
Repeati73 – 10533TPR 3Add
BLAST
Repeati122 – 15534TPR 4Add
BLAST
Domaini285 – 36581PB1PROSITE-ProRule annotationAdd
BLAST
Domaini369 – 42860SH3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 223223Mediates interaction with RAC1By similarityAdd
BLAST

Domaini

The SH3 domain mediates interaction with NOXO1 and NCF1 and has autoregulatory function.By similarity
The TPR repeats mediate interaction with RAC1.By similarity

Sequence similaritiesi

Belongs to the NCF2/NOXA1 family.Curated
Contains 1 PB1 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 4 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain, TPR repeat

Phylogenomic databases

eggNOGiKOG4225. Eukaryota.
ENOG41110AD. LUCA.
GeneTreeiENSGT00530000063843.
HOGENOMiHOG000237312.
HOVERGENiHBG098043.
InParanoidiQ8CJ00.
OMAiFQLERFQ.
OrthoDBiEOG7S7SF5.
PhylomeDBiQ8CJ00.
TreeFamiTF329087.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001452. SH3_domain.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00326. SH3. 1 hit.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS51745. PB1. 1 hit.
PS50002. SH3. 1 hit.
PS50005. TPR. 2 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CJ00-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSLGDQIRD WHRGVLAVAR EDWDSALCFF SDVREPLARM YFNRGCVHLM
60 70 80 90 100
AGDPEAALRA FDQAVTKDTC MAVGFLQRGV ANFQLQRFQE AVSDFQLALA
110 120 130 140 150
QLRDNAVIDY TQLGLNFKLQ AWEVLYNMAS AQCQAGLWTK AANTLVEAIS
160 170 180 190 200
KWPEGAQDIL DIAMDKVQKQ VPLQLQQVPK GEVFQPPRRY LKHLEPMDFL
210 220 230 240 250
GKAKVVASVI PDDHNAQPQQ RSQAEHAGHQ PSSSMCKRVL STTGGHTSPG
260 270 280 290 300
LYDSLLASRR PGPGPSEVSS GSEGAATKDP ESLVTVTVQC HFTVPLKVPR
310 320 330 340 350
GTGLSSFQTL LAQALLHQTQ TGQLSYKAPG EERSWIPIST EESLQSIWRN
360 370 380 390 400
VPVGPGGLQL QCQGVWGRPV LYQVVAQYNY RAQRPEDLDF HQGDTVDVLC
410 420 430 440
EVDEAWLEGH RDGCVGIFPK CFVVPAGAYV EAMLVLGPQP GDQN
Length:444
Mass (Da):48,880
Last modified:March 1, 2003 - v1
Checksum:iFD46EB298CD2E48F
GO
Isoform 2 (identifier: Q8CJ00-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     237-244: Missing.

Note: No experimental confirmation available.
Show »
Length:436
Mass (Da):48,037
Checksum:iDDF9D862F24C7F0B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti235 – 2351M → T in AAP13481 (PubMed:12657628).Curated
Sequence conflicti235 – 2351M → T in AAH47532 (PubMed:15489334).Curated
Sequence conflicti259 – 2591R → G in AAP13481 (PubMed:12657628).Curated
Sequence conflicti259 – 2591R → G in AAH47532 (PubMed:15489334).Curated
Sequence conflicti390 – 3901F → S in BAC39832 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei237 – 2448Missing in isoform 2. 1 PublicationVSP_030337

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF539798 mRNA. Translation: AAN75143.1.
AY255770 mRNA. Translation: AAP13481.1.
AK087263 mRNA. Translation: BAC39832.1.
AL732585 Genomic DNA. Translation: CAM17999.1.
AL732585 Genomic DNA. Translation: CAM18000.1.
BC047532 mRNA. Translation: AAH47532.1.
CCDSiCCDS15749.1. [Q8CJ00-1]
CCDS50524.1. [Q8CJ00-2]
RefSeqiNP_001157098.1. NM_001163626.1. [Q8CJ00-2]
NP_757341.3. NM_172204.4. [Q8CJ00-1]
UniGeneiMm.218774.

Genome annotation databases

EnsembliENSMUST00000044018; ENSMUSP00000037423; ENSMUSG00000036805. [Q8CJ00-1]
ENSMUST00000114373; ENSMUSP00000110014; ENSMUSG00000036805. [Q8CJ00-2]
GeneIDi241275.
KEGGimmu:241275.
UCSCiuc008iqh.2. mouse. [Q8CJ00-1]
uc008iqi.2. mouse. [Q8CJ00-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF539798 mRNA. Translation: AAN75143.1.
AY255770 mRNA. Translation: AAP13481.1.
AK087263 mRNA. Translation: BAC39832.1.
AL732585 Genomic DNA. Translation: CAM17999.1.
AL732585 Genomic DNA. Translation: CAM18000.1.
BC047532 mRNA. Translation: AAH47532.1.
CCDSiCCDS15749.1. [Q8CJ00-1]
CCDS50524.1. [Q8CJ00-2]
RefSeqiNP_001157098.1. NM_001163626.1. [Q8CJ00-2]
NP_757341.3. NM_172204.4. [Q8CJ00-1]
UniGeneiMm.218774.

3D structure databases

ProteinModelPortaliQ8CJ00.
SMRiQ8CJ00. Positions 3-203, 373-423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037423.

PTM databases

iPTMnetiQ8CJ00.
PhosphoSiteiQ8CJ00.

Proteomic databases

PaxDbiQ8CJ00.
PRIDEiQ8CJ00.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044018; ENSMUSP00000037423; ENSMUSG00000036805. [Q8CJ00-1]
ENSMUST00000114373; ENSMUSP00000110014; ENSMUSG00000036805. [Q8CJ00-2]
GeneIDi241275.
KEGGimmu:241275.
UCSCiuc008iqh.2. mouse. [Q8CJ00-1]
uc008iqi.2. mouse. [Q8CJ00-2]

Organism-specific databases

CTDi10811.
MGIiMGI:2449980. Noxa1.

Phylogenomic databases

eggNOGiKOG4225. Eukaryota.
ENOG41110AD. LUCA.
GeneTreeiENSGT00530000063843.
HOGENOMiHOG000237312.
HOVERGENiHBG098043.
InParanoidiQ8CJ00.
OMAiFQLERFQ.
OrthoDBiEOG7S7SF5.
PhylomeDBiQ8CJ00.
TreeFamiTF329087.

Enzyme and pathway databases

ReactomeiR-MMU-5668599. RHO GTPases Activate NADPH Oxidases.

Miscellaneous databases

NextBioi384957.
PROiQ8CJ00.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CJ00.
CleanExiMM_NOXA1.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001452. SH3_domain.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00326. SH3. 1 hit.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS51745. PB1. 1 hit.
PS50002. SH3. 1 hit.
PS50005. TPR. 2 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two novel proteins activate superoxide generation by the NADPH oxidase NOX1."
    Banfi B., Clark R.A., Steger K., Krause K.-H.
    J. Biol. Chem. 278:3510-3513(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION AS NOX1 ACTIVATOR, TISSUE SPECIFICITY.
    Strain: BALB/cJ.
    Tissue: Colon.
  2. "Proteins homologous to p47phox and p67phox support superoxide production by NAD(P)H oxidase 1 in colon epithelial cells."
    Geiszt M., Lekstrom K., Witta J., Leto T.L.
    J. Biol. Chem. 278:20006-20012(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: FVB/N.
    Tissue: Colon.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Lung.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Colon.
  6. Cited for: FUNCTION.
  7. "Alternative mRNA splice forms of NOXO1: differential tissue expression and regulation of Nox1 and Nox3."
    Cheng G., Lambeth J.D.
    Gene 356:118-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Noxa1 is a central component of the smooth muscle NADPH oxidase in mice."
    Ambasta R.K., Schreiber J.G., Janiszewski M., Busse R., Brandes R.P.
    Free Radic. Biol. Med. 41:193-201(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NOX1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNOXA1_MOUSE
AccessioniPrimary (citable) accession number: Q8CJ00
Secondary accession number(s): A2AJA1, Q80SY2, Q8BU68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2003
Last modified: May 11, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.