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Protein

NADPH oxidase 1

Gene

Nox1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H+ ions as part of its electron transport mechanism.1 Publication

Caution

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

The oxidase activity is potentiated by NOXA1 and NOXO1.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi129Iron (heme axial ligand)Curated1
Metal bindingi143Iron (heme axial ligand)Curated1
Metal bindingi236Iron (heme axial ligand)Curated1
Metal bindingi248Iron (heme axial ligand)Curated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi365 – 371FADSequence analysis7

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: MGI
  • cell migration Source: MGI
  • cellular response to hyperoxia Source: MGI
  • cellular stress response to acidic pH Source: MGI
  • extracellular matrix organization Source: MGI
  • hydrogen peroxide metabolic process Source: MGI
  • intracellular pH elevation Source: MGI
  • oxidation-reduction process Source: MGI
  • oxygen metabolic process Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of integrin biosynthetic process Source: MGI
  • positive regulation of JNK cascade Source: MGI
  • positive regulation of MAPK cascade Source: MGI
  • positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of vascular endothelial growth factor production Source: Ensembl
  • regulation of systemic arterial blood pressure by renin-angiotensin Source: MGI
  • superoxide anion generation Source: MGI
  • superoxide metabolic process Source: MGI

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.6.3.1 3474
ReactomeiR-MMU-5668599 RHO GTPases Activate NADPH Oxidases

Protein family/group databases

PeroxiBasei5963 MmNOx01

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH oxidase 1 (EC:1.-.-.-)
Short name:
NOX-1
Gene namesi
Name:Nox1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2450016 Nox1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36CytoplasmicSequence analysisAdd BLAST36
Transmembranei37 – 59Helical; Name=1Sequence analysisAdd BLAST23
Topological domaini60 – 72ExtracellularSequence analysisAdd BLAST13
Transmembranei73 – 97Helical; Name=2Sequence analysisAdd BLAST25
Topological domaini98 – 130CytoplasmicSequence analysisAdd BLAST33
Transmembranei131 – 151Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini152 – 195ExtracellularSequence analysisAdd BLAST44
Transmembranei196 – 216Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini217 – 234CytoplasmicSequence analysisAdd BLAST18
Transmembranei235 – 255Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini256 – 423ExtracellularSequence analysisAdd BLAST168
Transmembranei424 – 444Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini445 – 591CytoplasmicSequence analysisAdd BLAST147

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003229821 – 591NADPH oxidase 1Add BLAST591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi189N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi269N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8CIZ9
PRIDEiQ8CIZ9

PTM databases

iPTMnetiQ8CIZ9
PhosphoSitePlusiQ8CIZ9

Expressioni

Tissue specificityi

Isoform 2 expressed in colon and vascular smooth muscle cells (VSMC).1 Publication

Inductioni

Isoform 1 (c-type) induced in VSMC by angiotensin II and injury to the artery.1 Publication

Gene expression databases

BgeeiENSMUSG00000031257
CleanExiMM_NOX1
ExpressionAtlasiQ8CIZ9 baseline and differential

Interactioni

Subunit structurei

NOX1, NOXA1, NOXO1, RAC1 and CYBA forms a functional multimeric complex supporting ROS production. Interacts with NOXA1 and NOXO1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033610

Structurei

3D structure databases

ProteinModelPortaliQ8CIZ9
SMRiQ8CIZ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 316Ferric oxidoreductaseAdd BLAST235
Domaini317 – 418FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST102

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni424 – 563Interaction with NOXO1By similarityAdd BLAST140

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039 Eukaryota
ENOG410XNZY LUCA
GeneTreeiENSGT00550000074350
HOGENOMiHOG000216669
HOVERGENiHBG003760
InParanoidiQ8CIZ9
KOiK08008
OMAiIHIRAAG
OrthoDBiEOG091G09RV
PhylomeDBiQ8CIZ9
TreeFamiTF105354

Family and domain databases

InterProiView protein in InterPro
IPR000778 Cyt_b245_heavy_chain
IPR013112 FAD-bd_8
IPR017927 Fd_Rdtase_FAD-bd
IPR013130 Fe3_Rdtase_TM_dom
IPR013121 Fe_red_NAD-bd_6
IPR029650 NOX1
IPR017938 Riboflavin_synthase-like_b-brl
PANTHERiPTHR11972:SF71 PTHR11972:SF71, 1 hit
PfamiView protein in Pfam
PF08022 FAD_binding_8, 1 hit
PF01794 Ferric_reduct, 1 hit
PF08030 NAD_binding_6, 1 hit
PRINTSiPR00466 GP91PHOX
SUPFAMiSSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CIZ9-1) [UniParc]FASTAAdd to basket
Also known as: f-type, c-type

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGELRGSRG PLQRIQIAPR EAPNLHLTMG NWLVNHWLSV LFLVSWLGLN
60 70 80 90 100
IFLFVYAFLN YEKSDKYYYT REILGTALAL ARASALCLNF NSMMILIPVC
110 120 130 140 150
RNLLSFLRGT CSFCNRTLRK PLDHNLTFHK LVAYMICIFT VIHIIAHLFN
160 170 180 190 200
FERYRRSQQA MDGSLASVLS SLSHPEKEDS WLNPIQSPNM TVMYAAFTSI
210 220 230 240 250
AGLTGVIATV ALVLMVTSAM EFIRRNYFEL FWYTHHLFIV YIICLGIHGL
260 270 280 290 300
GGIVRGQTEE SLGESHPHNC SHSFHEWDDH KGSCRHPHFA GHPPESWKWI
310 320 330 340 350
LAPIAFYIFE RILRFYRSQQ KVVITKVVMH PSNVLELQMR KRGFSMEVGQ
360 370 380 390 400
YIFVNCPSIS FLEWHPFTLT SAPEEEFFSV HIRAAGDWTR NLIRTFEQQH
410 420 430 440 450
SPMPRIEVDG PFGTVSEDVF QYEVAVLVGA GIGVTPFASI LKSIWYKFQR
460 470 480 490 500
ADNKLKTQKI YFYWICRETG AFAWFNNLLN SLEQEMEELG KMDFLNYRLF
510 520 530 540 550
LTGWDSNIAG HAALNFDRAT DILTGLKQKT SFGRPMWDNE FSRIATAHPK
560 570 580 590
SAVGVFLCGP RTLAKSLRKR CQRYSSLDPR KVQFYFNKET F
Note: Product of f-type and c-type mRNA, which differ only in 5'-UTR.
Length:591
Mass (Da):68,193
Last modified:January 19, 2010 - v2
Checksum:iB2D4AE54186A066B
GO
Isoform 2 (identifier: Q8CIZ9-2) [UniParc]FASTAAdd to basket
Also known as: a-type

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.

Show »
Length:563
Mass (Da):65,128
Checksum:i985620FDFBB75F65
GO
Isoform 3 (identifier: Q8CIZ9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.
     460-508: Missing.

Note: Gene prediction based on human ortholog.
Show »
Length:514
Mass (Da):59,143
Checksum:i21F89266C87A1911
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0385741 – 28Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST28
Alternative sequenceiVSP_038575460 – 508Missing in isoform 3. CuratedAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF539799 mRNA Translation: AAN75144.1
AK136432 mRNA Translation: BAE22974.1
AL671915 Genomic DNA Translation: CAM16733.1
AL671915 Genomic DNA Translation: CAM16734.1
AB206383 mRNA Translation: BAF03561.1
AB206384 mRNA Translation: BAF03562.1
AB206385 mRNA Translation: BAF03563.1
AY174116 mRNA Translation: AAO20852.1
CCDSiCCDS30390.1 [Q8CIZ9-2]
RefSeqiNP_757340.1, NM_172203.2 [Q8CIZ9-2]
XP_006528578.1, XM_006528515.3 [Q8CIZ9-1]
UniGeneiMm.233865
Mm.455133
Mm.67938

Genome annotation databases

EnsembliENSMUST00000033610; ENSMUSP00000033610; ENSMUSG00000031257 [Q8CIZ9-2]
ENSMUST00000113275; ENSMUSP00000108900; ENSMUSG00000031257 [Q8CIZ9-3]
GeneIDi237038
KEGGimmu:237038
UCSCiuc009ufl.2 mouse [Q8CIZ9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNOX1_MOUSE
AccessioniPrimary (citable) accession number: Q8CIZ9
Secondary accession number(s): A2AEK5
, Q0KKX3, Q0KKX5, Q811U2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: January 19, 2010
Last modified: April 25, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome