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Protein

Transcription factor Ovo-like 2

Gene

Ovol2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Zinc-finger transcription repressor factor. Plays a critical role to maintain the identity of epithelial lineages by suppressing epithelial-to mesenchymal transition mainly through the up-regulation of ZEB1 expression. Positively regulates neuronal differentiation. Suppresses cell cycling and terminal differentiation of keratinocytes by directly repressing MYC and NOTCH1.5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri118 – 14023C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri146 – 16823C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri174 – 19724C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri213 – 23624C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding Source: MGI
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  • angiogenesis Source: MGI
  • dorsal/ventral pattern formation Source: MGI
  • embryonic digestive tract morphogenesis Source: MGI
  • endocardium formation Source: MGI
  • epidermal cell differentiation Source: UniProtKB
  • epidermis development Source: MGI
  • heart development Source: MGI
  • heart looping Source: MGI
  • heart trabecula formation Source: MGI
  • labyrinthine layer blood vessel development Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of epithelial to mesenchymal transition Source: UniProtKB
  • negative regulation of keratinocyte differentiation Source: UniProtKB
  • negative regulation of Notch signaling pathway Source: UniProtKB
  • negative regulation of stem cell proliferation Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription by competitive promoter binding Source: MGI
  • neural crest cell migration Source: MGI
  • neural fold formation Source: MGI
  • positive regulation of keratinocyte differentiation Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • regulation of cell cycle Source: UniProtKB
  • regulation of keratinocyte proliferation Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Ovo-like 2
Short name:
mOvo2
Alternative name(s):
Zinc finger OVO2
Zinc finger protein 339
Zinc finger protein mOVO
Gene namesi
Name:Ovol2
Synonyms:Ovo2, Zfp339, Znf339
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1338039. Ovol2.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant embryos are small and die at E9.5-E10.5 with an open neural tube, impaired extra-embryonic and embryonic vascularization, abnormal cardiogenesis and placental defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Transcription factor Ovo-like 2PRO_0000047014Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei268 – 2681PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CIV7.
PRIDEiQ8CIV7.

PTM databases

PhosphoSiteiQ8CIV7.

Expressioni

Tissue specificityi

Expressed highly in testis, specifically in spermatocytes. Expressed also in skin and at lower levels in the ovary.3 Publications

Developmental stagei

Expressed during early-mid embryogenesis, particularly in the inner cell mass at E3.5, in epiblast at E6.5, and at later stages in ectodermally derived tissues such as the rostral surface ectoderm. Up-regulated during prepupertal testis development.2 Publications

Inductioni

Down-regulated during embryonic stem cell neural differentiation and up-regulated by BMP4.1 Publication

Gene expression databases

BgeeiQ8CIV7.
CleanExiMM_OVOL2.
GenevisibleiQ8CIV7. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044026.

Structurei

3D structure databases

ProteinModelPortaliQ8CIV7.
SMRiQ8CIV7. Positions 65-219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri118 – 14023C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri146 – 16823C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri174 – 19724C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri213 – 23624C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG3576. Eukaryota.
ENOG4111H28. LUCA.
GeneTreeiENSGT00740000115492.
HOGENOMiHOG000231694.
HOVERGENiHBG053384.
InParanoidiQ8CIV7.
KOiK09216.
OMAiSTHQENT.
OrthoDBiEOG786H2K.
TreeFamiTF337552.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR027775. C2H2_Znf_fam.
IPR027755. TF_Ovo-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR10032. PTHR10032. 1 hit.
PTHR10032:SF193. PTHR10032:SF193. 1 hit.
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CIV7-1) [UniParc]FASTAAdd to basket

Also known as: MOVO-A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPKVFLVKRR SPGVSVRSWD ELPDDKRADT YIPVSLGCLL RDPPEDCRSD
60 70 80 90 100
GGSSSGCSSS AGEPGGAESS SSPRAPEPET PELHDAQGTD GHLAAMQRPV
110 120 130 140 150
ARSKIKFTTG TCDNSVIHNC DLCGKSFRLQ RMLNRHLKCH NQVKRHLCTF
160 170 180 190 200
CGKGFNDTFD LKRHVRTHTG IRPYKCEVCN KAFTQRCSLE SHLKKIHGVQ
210 220 230 240 250
QQYAYKQRRD KLYVCEDCGY TGPTQEDLYL HVNSDHPGST FLKKTSKKLA
260 270
ALMQNKLTSP LQENSTLSEE EEKK
Length:274
Mass (Da):30,685
Last modified:June 28, 2011 - v2
Checksum:i8BE48CE57A9F32E4
GO
Isoform 2 (identifier: Q8CIV7-2) [UniParc]FASTAAdd to basket

Also known as: MOVO-C

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.
     34-34: V → M

Show »
Length:241
Mass (Da):26,875
Checksum:i50459C1B22AD2C7C
GO
Isoform 3 (identifier: Q8CIV7-3) [UniParc]FASTAAdd to basket

Also known as: MOVO-B

The sequence of this isoform differs from the canonical sequence as follows:
     1-95: Missing.

Note: Major form in the testis.
Show »
Length:179
Mass (Da):20,781
Checksum:i4C2734C2F9A8F0BE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 671A → S in AAM11991 (PubMed:12213202).Curated
Sequence conflicti67 – 671A → S in AAM11992 (PubMed:12213202).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9595Missing in isoform 3. 3 PublicationsVSP_013576Add
BLAST
Alternative sequencei1 – 3333Missing in isoform 2. 2 PublicationsVSP_013577Add
BLAST
Alternative sequencei34 – 341V → M in isoform 2. 2 PublicationsVSP_013578

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY090537 mRNA. Translation: AAM11991.1.
AY090538 mRNA. Translation: AAM11992.1.
AB101294 mRNA. Translation: BAD29939.1.
AB101295 mRNA. Translation: BAD29940.1.
AB101296 mRNA. Translation: BAD29941.1.
AB101299 Genomic DNA. Translation: BAD29942.1.
AB101299 Genomic DNA. Translation: BAD29943.1.
AB101299 Genomic DNA. Translation: BAD29944.1.
AK007360 mRNA. Translation: BAB24985.1.
AL808123 Genomic DNA. Translation: CAM26789.1.
CH466519 Genomic DNA. Translation: EDL28455.1.
BC031771 mRNA. Translation: AAH31771.1.
BC057210 mRNA. Translation: AAH57210.1.
BC094445 mRNA. Translation: AAH94445.1.
CCDSiCCDS16817.1. [Q8CIV7-1]
CCDS16818.1. [Q8CIV7-2]
RefSeqiNP_081200.2. NM_026924.3. [Q8CIV7-1]
NP_694455.2. NM_152947.2. [Q8CIV7-2]
UniGeneiMm.252750.

Genome annotation databases

EnsembliENSMUST00000037423; ENSMUSP00000044026; ENSMUSG00000037279. [Q8CIV7-1]
ENSMUST00000103171; ENSMUSP00000099460; ENSMUSG00000037279. [Q8CIV7-2]
GeneIDi107586.
KEGGimmu:107586.
UCSCiuc008mqv.1. mouse. [Q8CIV7-2]
uc008mqw.1. mouse. [Q8CIV7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY090537 mRNA. Translation: AAM11991.1.
AY090538 mRNA. Translation: AAM11992.1.
AB101294 mRNA. Translation: BAD29939.1.
AB101295 mRNA. Translation: BAD29940.1.
AB101296 mRNA. Translation: BAD29941.1.
AB101299 Genomic DNA. Translation: BAD29942.1.
AB101299 Genomic DNA. Translation: BAD29943.1.
AB101299 Genomic DNA. Translation: BAD29944.1.
AK007360 mRNA. Translation: BAB24985.1.
AL808123 Genomic DNA. Translation: CAM26789.1.
CH466519 Genomic DNA. Translation: EDL28455.1.
BC031771 mRNA. Translation: AAH31771.1.
BC057210 mRNA. Translation: AAH57210.1.
BC094445 mRNA. Translation: AAH94445.1.
CCDSiCCDS16817.1. [Q8CIV7-1]
CCDS16818.1. [Q8CIV7-2]
RefSeqiNP_081200.2. NM_026924.3. [Q8CIV7-1]
NP_694455.2. NM_152947.2. [Q8CIV7-2]
UniGeneiMm.252750.

3D structure databases

ProteinModelPortaliQ8CIV7.
SMRiQ8CIV7. Positions 65-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044026.

PTM databases

PhosphoSiteiQ8CIV7.

Proteomic databases

PaxDbiQ8CIV7.
PRIDEiQ8CIV7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037423; ENSMUSP00000044026; ENSMUSG00000037279. [Q8CIV7-1]
ENSMUST00000103171; ENSMUSP00000099460; ENSMUSG00000037279. [Q8CIV7-2]
GeneIDi107586.
KEGGimmu:107586.
UCSCiuc008mqv.1. mouse. [Q8CIV7-2]
uc008mqw.1. mouse. [Q8CIV7-1]

Organism-specific databases

CTDi58495.
MGIiMGI:1338039. Ovol2.

Phylogenomic databases

eggNOGiKOG3576. Eukaryota.
ENOG4111H28. LUCA.
GeneTreeiENSGT00740000115492.
HOGENOMiHOG000231694.
HOVERGENiHBG053384.
InParanoidiQ8CIV7.
KOiK09216.
OMAiSTHQENT.
OrthoDBiEOG786H2K.
TreeFamiTF337552.

Miscellaneous databases

PROiQ8CIV7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CIV7.
CleanExiMM_OVOL2.
GenevisibleiQ8CIV7. MM.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR027775. C2H2_Znf_fam.
IPR027755. TF_Ovo-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR10032. PTHR10032. 1 hit.
PTHR10032:SF193. PTHR10032:SF193. 1 hit.
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of murine novel zinc finger proteins highly homologous to Drosophila ovo gene product in testis."
    Masu Y., Ikeda S., Okuda-Ashitaka E., Sato E., Ito S.
    FEBS Lett. 421:224-228(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  2. "Ovol2, a mammalian homolog of Drosophila ovo: gene structure, chromosomal mapping, and aberrant expression in blind-sterile mice."
    Li B., Dai Q., Li L., Nair M., Mackay D.R., Dai X.
    Genomics 80:319-325(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Strain: CD-1.
  3. "Characterization of the isoforms of MOVO zinc finger protein, a mouse homologue of Drosophila Ovo, as a transcription factors."
    Unezaki S., Nishizawa M., Okuda-Ashitaka E., Masu Y., Mukai M., Kobayashi S., Sawamoto K., Okano H., Ito S.
    Gene 336:47-58(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: ICR.
    Tissue: Liver and Testis.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Pancreas.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N and NMRI.
    Tissue: Colon and Mammary tumor.
  8. "The mouse Ovol2 gene is required for cranial neural tube development."
    Mackay D.R., Hu M., Li B., Rheaume C., Dai X.
    Dev. Biol. 291:38-52(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, FUNCTION.
  9. "The zinc finger transcription factor Ovol2 acts downstream of the bone morphogenetic protein pathway to regulate the cell fate decision between neuroectoderm and mesendoderm."
    Zhang T., Zhu Q., Xie Z., Chen Y., Qiao Y., Li L., Jing N.
    J. Biol. Chem. 288:6166-6177(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, FUNCTION.
  10. "Transcriptional mechanisms link epithelial plasticity to adhesion and differentiation of epidermal progenitor cells."
    Lee B., Villarreal-Ponce A., Fallahi M., Ovadia J., Sun P., Yu Q.C., Ito S., Sinha S., Nie Q., Dai X.
    Dev. Cell 29:47-58(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Mammary morphogenesis and regeneration require the inhibition of EMT at terminal end buds by Ovol2 transcriptional repressor."
    Watanabe K., Villarreal-Ponce A., Sun P., Salmans M.L., Fallahi M., Andersen B., Dai X.
    Dev. Cell 29:59-74(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiOVOL2_MOUSE
AccessioniPrimary (citable) accession number: Q8CIV7
Secondary accession number(s): Q505Q7
, Q6F661, Q8CIV6, Q8K0D6, Q9D949
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 28, 2011
Last modified: July 6, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.