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Protein

Transcription factor Ovo-like 2

Gene

Ovol2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Zinc-finger transcription repressor factor (PubMed:15225875, PubMed:23319585). Plays a critical role in maintaining the identity of epithelial lineages by suppressing epithelial-to mesenchymal transition (EMT) mainly through the repression of ZEB1, an EMT inducer (PubMed:24735878, PubMed:24735879). Positively regulates neuronal differentiation (PubMed:16423343, PubMed:23319585). Suppresses cell cycling and terminal differentiation of keratinocytes by directly repressing MYC and NOTCH1 (By similarity). Important for the correct development of primordial germ cells in embryos (PubMed:28059165).By similarity6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri118 – 140C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri146 – 168C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri174 – 197C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri213 – 236C2H2-type 4PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding Source: MGI
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  • angiogenesis Source: MGI
  • dorsal/ventral pattern formation Source: MGI
  • embryonic digestive tract morphogenesis Source: MGI
  • endocardium formation Source: MGI
  • epidermal cell differentiation Source: UniProtKB
  • epidermis development Source: MGI
  • heart development Source: MGI
  • heart looping Source: MGI
  • heart trabecula formation Source: MGI
  • labyrinthine layer blood vessel development Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of epithelial to mesenchymal transition Source: UniProtKB
  • negative regulation of keratinocyte differentiation Source: UniProtKB
  • negative regulation of Notch signaling pathway Source: UniProtKB
  • negative regulation of stem cell proliferation Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription by competitive promoter binding Source: MGI
  • neural crest cell migration Source: MGI
  • neural fold formation Source: MGI
  • positive regulation of keratinocyte differentiation Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • regulation of cell cycle Source: UniProtKB
  • regulation of keratinocyte proliferation Source: BHF-UCL

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Ovo-like 2
Short name:
mOvo2
Alternative name(s):
Zinc finger OVO2
Zinc finger protein 339
Zinc finger protein mOVO
Gene namesi
Name:Ovol2
Synonyms:Ovo2, Zfp339, Znf339
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1338039. Ovol2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant embryos are small and die at E9.5-E10.5 with an open neural tube, impaired extra-embryonic and embryonic vascularization, abnormal cardiogenesis and placental defects (PubMed:16423343). Reduced number of primordial germ cells in E8.0 embryos (PubMed:28059165).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000470141 – 274Transcription factor Ovo-like 2Add BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei268PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CIV7.
PRIDEiQ8CIV7.

PTM databases

PhosphoSitePlusiQ8CIV7.

Expressioni

Tissue specificityi

Expressed highly in testis, specifically in spermatocytes. Expressed also in skin and at lower levels in the ovary.3 Publications

Developmental stagei

Expressed during early-mid embryogenesis, particularly in the inner cell mass at E3.5, in epiblast at E6.5, and at later stages in ectodermally derived tissues such as the rostral surface ectoderm (PubMed:16423343). Expressed in embryonic stem cells, epiblasts of E6.4 embryos and primordial germ cells (PGCs) (PubMed:28059165). High expression levels in PGCs of E8.5 embryos decrease over embryogenesis (PubMed:28059165). Up-regulated during prepupertal testis development (PubMed:15225875).3 Publications

Inductioni

Down-regulated during embryonic stem cell neural differentiation and up-regulated by BMP4.1 Publication

Gene expression databases

BgeeiENSMUSG00000037279.
CleanExiMM_OVOL2.
GenevisibleiQ8CIV7. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044026.

Structurei

3D structure databases

ProteinModelPortaliQ8CIV7.
SMRiQ8CIV7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri118 – 140C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri146 – 168C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri174 – 197C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri213 – 236C2H2-type 4PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG3576. Eukaryota.
ENOG4111H28. LUCA.
GeneTreeiENSGT00860000133786.
HOGENOMiHOG000231694.
HOVERGENiHBG053384.
InParanoidiQ8CIV7.
KOiK09216.
OMAiHPGSTFL.
OrthoDBiEOG091G0LB1.
TreeFamiTF337552.

Family and domain databases

InterProiView protein in InterPro
IPR027756. Ovo-like.
IPR036236. Znf_C2H2_sf.
IPR013087. Znf_C2H2_type.
PANTHERiPTHR10032. PTHR10032. 1 hit.
SMARTiView protein in SMART
SM00355. ZnF_C2H2. 4 hits.
SUPFAMiSSF57667. SSF57667. 2 hits.
PROSITEiView protein in PROSITE
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CIV7-1) [UniParc]FASTAAdd to basket
Also known as: MOVO-A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPKVFLVKRR SPGVSVRSWD ELPDDKRADT YIPVSLGCLL RDPPEDCRSD
60 70 80 90 100
GGSSSGCSSS AGEPGGAESS SSPRAPEPET PELHDAQGTD GHLAAMQRPV
110 120 130 140 150
ARSKIKFTTG TCDNSVIHNC DLCGKSFRLQ RMLNRHLKCH NQVKRHLCTF
160 170 180 190 200
CGKGFNDTFD LKRHVRTHTG IRPYKCEVCN KAFTQRCSLE SHLKKIHGVQ
210 220 230 240 250
QQYAYKQRRD KLYVCEDCGY TGPTQEDLYL HVNSDHPGST FLKKTSKKLA
260 270
ALMQNKLTSP LQENSTLSEE EEKK
Length:274
Mass (Da):30,685
Last modified:June 28, 2011 - v2
Checksum:i8BE48CE57A9F32E4
GO
Isoform 2 (identifier: Q8CIV7-2) [UniParc]FASTAAdd to basket
Also known as: MOVO-C

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.
     34-34: V → M

Show »
Length:241
Mass (Da):26,875
Checksum:i50459C1B22AD2C7C
GO
Isoform 3 (identifier: Q8CIV7-3) [UniParc]FASTAAdd to basket
Also known as: MOVO-B

The sequence of this isoform differs from the canonical sequence as follows:
     1-95: Missing.

Note: Major form in the testis.
Show »
Length:179
Mass (Da):20,781
Checksum:i4C2734C2F9A8F0BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67A → S in AAM11991 (PubMed:12213202).Curated1
Sequence conflicti67A → S in AAM11992 (PubMed:12213202).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0135761 – 95Missing in isoform 3. 3 PublicationsAdd BLAST95
Alternative sequenceiVSP_0135771 – 33Missing in isoform 2. 2 PublicationsAdd BLAST33
Alternative sequenceiVSP_01357834V → M in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY090537 mRNA. Translation: AAM11991.1.
AY090538 mRNA. Translation: AAM11992.1.
AB101294 mRNA. Translation: BAD29939.1.
AB101295 mRNA. Translation: BAD29940.1.
AB101296 mRNA. Translation: BAD29941.1.
AB101299 Genomic DNA. Translation: BAD29942.1.
AB101299 Genomic DNA. Translation: BAD29943.1.
AB101299 Genomic DNA. Translation: BAD29944.1.
AK007360 mRNA. Translation: BAB24985.1.
AL808123 Genomic DNA. Translation: CAM26789.1.
CH466519 Genomic DNA. Translation: EDL28455.1.
BC031771 mRNA. Translation: AAH31771.1.
BC057210 mRNA. Translation: AAH57210.1.
BC094445 mRNA. Translation: AAH94445.1.
CCDSiCCDS16817.1. [Q8CIV7-1]
CCDS16818.1. [Q8CIV7-2]
RefSeqiNP_081200.2. NM_026924.3. [Q8CIV7-1]
NP_694455.2. NM_152947.2. [Q8CIV7-2]
UniGeneiMm.252750.

Genome annotation databases

EnsembliENSMUST00000037423; ENSMUSP00000044026; ENSMUSG00000037279. [Q8CIV7-1]
ENSMUST00000103171; ENSMUSP00000099460; ENSMUSG00000037279. [Q8CIV7-2]
GeneIDi107586.
KEGGimmu:107586.
UCSCiuc008mqv.1. mouse. [Q8CIV7-2]
uc008mqw.1. mouse. [Q8CIV7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiOVOL2_MOUSE
AccessioniPrimary (citable) accession number: Q8CIV7
Secondary accession number(s): Q505Q7
, Q6F661, Q8CIV6, Q8K0D6, Q9D949
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 28, 2011
Last modified: October 25, 2017
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families