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Q8CIS0 (CAR11_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Caspase recruitment domain-containing protein 11
Gene names
Name:Card11
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1159 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10 By similarity. Ref.5

Subunit structure

Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. CARD11 and BCL10 bind to each other by CARD-CARD interaction. Interacts (via PDZ domain) with DPP4 (via cytoplasmic tail) By similarity.

Subcellular location

Cytoplasm By similarity. Membrane raft By similarity. Note: Colocalized with DPP4 in membrane rafts By similarity.

Post-translational modification

Phosphorylation at Ser-564, Ser-649 and Ser-657 by PRKCB and PRKCQ leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.

Sequence similarities

Contains 1 CARD domain.

Contains 1 guanylate kinase-like domain.

Contains 1 PDZ (DHR) domain.

Caution

Supposed to contain a SH3 domain which is not detected by PROSITE, Pfam or SMART.

Sequence caution

The sequence BAD21384.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT cell costimulation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of B cell proliferation

Inferred from mutant phenotype PubMed 12867038. Source: MGI

positive regulation of I-kappaB kinase/NF-kappaB signaling

Inferred from mutant phenotype PubMed 12867038. Source: MGI

positive regulation of NF-kappaB transcription factor activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of T cell activation

Inferred from mutant phenotype PubMed 12867038. Source: MGI

positive regulation of T cell proliferation

Inferred from mutant phenotype PubMed 12867038. Source: MGI

positive regulation of cytokine production

Inferred from electronic annotation. Source: Ensembl

positive regulation of interleukin-2 biosynthetic process

Inferred from mutant phenotype PubMed 12867038. Source: MGI

regulation of B cell differentiation

Inferred from mutant phenotype PubMed 12867038. Source: MGI

regulation of T cell differentiation

Inferred from mutant phenotype PubMed 12867038. Source: MGI

regulation of apoptotic process

Inferred from electronic annotation. Source: InterPro

regulation of immune response

Inferred from mutant phenotype PubMed 12867038. Source: MGI

signal transduction

Inferred from direct assay Ref.1. Source: MGI

thymic T cell selection

Inferred from mutant phenotype PubMed 12867038. Source: MGI

   Cellular_componentT cell receptor complex

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane raft

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from direct assay PubMed 12867038. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8CIS0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8CIS0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     524-528: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11591159Caspase recruitment domain-containing protein 11
PRO_0000320102

Regions

Domain18 – 11093CARD
Domain672 – 76089PDZ
Domain978 – 1145168Guanylate kinase-like
Coiled coil176 – 449274 Potential
Compositional bias623 – 6275Poly-Ser

Amino acid modifications

Modified residue4661Phosphoserine By similarity
Modified residue5641Phosphoserine; by PKC/PRKCB and PKC/PRKCQ Ref.5
Modified residue5981Phosphoserine By similarity
Modified residue6491Phosphoserine; by PKC/PRKCB and PKC/PRKCQ Ref.5
Modified residue6571Phosphoserine; by PKC/PRKCB and PKC/PRKCQ Ref.5
Modified residue9301Phosphoserine By similarity

Natural variations

Alternative sequence524 – 5285Missing in isoform 2.
VSP_031595

Experimental info

Sequence conflict2581E → Q in AAN10150. Ref.1
Sequence conflict8601R → L in BAC29910. Ref.4

Secondary structure

................ 1159
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 26, 2008. Version 2.
Checksum: 2ED53905B99DD94E

FASTA1,159134,040
        10         20         30         40         50         60 
MPGGGPAMDD YMETLKDEEE ALWDNVECNR HMLSRYINPA KLTPYLRQCK VIDEQDEDEV 

        70         80         90        100        110        120 
LNAPMLPSKI NRAGRLLDIL HTKGQRGYVV FLESLEFYYP ELYKLVTGKE PTRRFSTIVV 

       130        140        150        160        170        180 
EEGHEGLTHF LMNEVIKLQQ QVKAKDLQRC ELLAKSRQLE DEKKQLSLIR VELLTFQERY 

       190        200        210        220        230        240 
YKMKEERDSY NDELVKVKDD NYNLAMRYAQ LSEEKNMAVM RSRDLQLEID QLKHRLNKME 

       250        260        270        280        290        300 
EECKLERNQS LKLKNDIENR PRKEQVLELE RENEMLKTKI QELQSIIQAG KRSLPDSDKA 

       310        320        330        340        350        360 
ILDILEHDRK EALEDRQELV NKIYNLQEEV RQAEELRDKY LEEKEDLELK CSTLGKDCEM 

       370        380        390        400        410        420 
YKHRMNTVML QLEEVERERD QAFHSRDEAQ TQYSQCLIEK DKYRKQIREL EEKNDEMRIE 

       430        440        450        460        470        480 
MVRREACIVN LESKLRRLSK DNGSLDQSLP RHLPATIISQ NLGDTSPRTN GQEADDSSTS 

       490        500        510        520        530        540 
EESPEDSKYF LPYHPPRRRM NLKGIQLQRA KSPISMKQAS EFQALMRTVK GHEEDFTDGS 

       550        560        570        580        590        600 
PSSSRSLPVT SSFSKMQPHR SRSSIMSITA EPPGNDSIVR RCKEDAPHRS TVEEDNDSCG 

       610        620        630        640        650        660 
FDALDLDDEN HERYSFGPPS IHSSSSSHQS EGLDAYDLEQ VNLMLRKFSL ERPFRPSVTS 

       670        680        690        700        710        720 
GGHVRGTGPL VQHTTLNGDG LITQLTLLGG NARGSFIHSV KPGSLAERAG LREGHQLLLL 

       730        740        750        760        770        780 
EGCIRGERQS VPLDACTKEE ARWTIQRCSG LITLHYKVNH EGYRKLLKEM EDGLITSGDS 

       790        800        810        820        830        840 
FYIRLNLNIS SQLDACSMSL KCDDVVHVLD TMYQDRHEWL CARVDPFTDQ DLDTGTIPSY 

       850        860        870        880        890        900 
SRAQQLLLVK LQRLVHRGNR EEADSAHHTL RSLRNTLQPE EMLSTSDPRV SPRLSRASFF 

       910        920        930        940        950        960 
FGQLLQFVSR SENKYKRMNS NERVRIISGS PLGSLSRSSL DATKLLTEKH EELDPENELS 

       970        980        990       1000       1010       1020 
RNLTLIPYSL VRAFHCERRR PVLFTPTMLA KTLVQKLLNS GGAMEFTICK SDIVTRDEFL 

      1030       1040       1050       1060       1070       1080 
RKQKTETIIY SREKNPNTFE CIVPANIEAV AAKNKHCLLE AGIGCVRDLI KCKVYPIVLL 

      1090       1100       1110       1120       1130       1140 
IRVSEKNIKR FRKLLPRPET EEEFLRVCRL KEKELEALPC LYATVEAEMW SSVEELLRVL 

      1150 
KDKIVEEQRK TIWVDEDQL 

« Hide

Isoform 2 [UniParc].

Checksum: CE5FA71361B2EB19
Show »

FASTA1,154133,468

References

« Hide 'large scale' references
[1]"CARD11 mediates factor-specific activation of NF-kappaB by the T cell receptor complex."
Pomerantz J.L., Denny E.M., Baltimore D.
EMBO J. 21:5184-5194(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Thymus.
[2]"Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.
DNA Res. 11:127-135(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: 129.
Tissue: Mammary tumor.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 611-1159.
Strain: C57BL/6J.
Tissue: Thymus.
[5]"Phosphorylation of the CARMA1 linker controls NF-kappaB activation."
Sommer K., Guo B., Pomerantz J.L., Bandaranayake A.D., Moreno-Garcia M.E., Ovechkina Y.L., Rawlings D.J.
Immunity 23:561-574(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION AT SER-564; SER-649 AND SER-657.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY135367 mRNA. Translation: AAN10150.1.
AK131134 mRNA. Translation: BAD21384.1. Different initiation.
BC096592 mRNA. Translation: AAH96592.1.
AK037968 mRNA. Translation: BAC29910.1.
RefSeqNP_780571.2. NM_175362.2.
UniGeneMm.46187.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4I16X-ray1.75A21-110[»]
ProteinModelPortalQ8CIS0.
SMRQ8CIS0. Positions 21-109, 401-445, 685-850.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid224389. 2 interactions.
DIPDIP-49692N.
IntActQ8CIS0. 4 interactions.
MINTMINT-1764942.
STRING10090.ENSMUSP00000082941.

Proteomic databases

PaxDbQ8CIS0.
PRIDEQ8CIS0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000085786; ENSMUSP00000082941; ENSMUSG00000036526. [Q8CIS0-2]
GeneID108723.
KEGGmmu:108723.
UCSCuc009aik.2. mouse. [Q8CIS0-2]
uc012egd.1. mouse. [Q8CIS0-1]

Organism-specific databases

CTD84433.
MGIMGI:1916978. Card11.

Phylogenomic databases

eggNOGNOG47732.
GeneTreeENSGT00530000063108.
HOGENOMHOG000111299.
HOVERGENHBG099790.
InParanoidQ8CIS0.
KOK07367.
OMAEALPCLY.
OrthoDBEOG747PH5.
PhylomeDBQ8CIS0.
TreeFamTF351139.

Enzyme and pathway databases

ReactomeREACT_98458. Immune System.

Gene expression databases

BgeeQ8CIS0.
CleanExMM_CARD11.
GenevestigatorQ8CIS0.

Family and domain databases

Gene3D1.10.533.10. 1 hit.
2.30.42.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
[Graphical view]
PfamPF00619. CARD. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF47986. SSF47986. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS50209. CARD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCARD11. mouse.
NextBio361261.
PROQ8CIS0.
SOURCESearch...

Entry information

Entry nameCAR11_MOUSE
AccessionPrimary (citable) accession number: Q8CIS0
Secondary accession number(s): Q4VA14, Q6KAS3, Q8BYV0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: April 16, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot