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Q8CIS0

- CAR11_MOUSE

UniProt

Q8CIS0 - CAR11_MOUSE

Protein

Caspase recruitment domain-containing protein 11

Gene

Card11

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10 By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. positive regulation of B cell proliferation Source: MGI
    2. positive regulation of cytokine production Source: Ensembl
    3. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
    4. positive regulation of interleukin-2 biosynthetic process Source: MGI
    5. positive regulation of NF-kappaB transcription factor activity Source: Ensembl
    6. positive regulation of T cell activation Source: MGI
    7. positive regulation of T cell proliferation Source: MGI
    8. regulation of apoptotic process Source: InterPro
    9. regulation of B cell differentiation Source: MGI
    10. regulation of immune response Source: MGI
    11. regulation of T cell differentiation Source: MGI
    12. signal transduction Source: MGI
    13. T cell costimulation Source: UniProtKB
    14. thymic T cell selection Source: MGI

    Enzyme and pathway databases

    ReactomeiREACT_199121. Activation of NF-kappaB in B cells.
    REACT_205561. FCERI mediated NF-kB activation.
    REACT_225145. Downstream TCR signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Caspase recruitment domain-containing protein 11
    Gene namesi
    Name:Card11
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1916978. Card11.

    Subcellular locationi

    Cytoplasm By similarity. Membrane raft By similarity
    Note: Colocalized with DPP4 in membrane rafts.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. immunological synapse Source: MGI
    3. membrane raft Source: UniProtKB-SubCell
    4. plasma membrane Source: MGI
    5. T cell receptor complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11591159Caspase recruitment domain-containing protein 11PRO_0000320102Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei466 – 4661PhosphoserineBy similarity
    Modified residuei564 – 5641Phosphoserine; by PKC/PRKCB and PKC/PRKCQ1 Publication
    Modified residuei598 – 5981PhosphoserineBy similarity
    Modified residuei649 – 6491Phosphoserine; by PKC/PRKCB and PKC/PRKCQ1 Publication
    Modified residuei657 – 6571Phosphoserine; by PKC/PRKCB and PKC/PRKCQ1 Publication
    Modified residuei930 – 9301PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation at Ser-564, Ser-649 and Ser-657 by PRKCB and PRKCQ leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8CIS0.
    PRIDEiQ8CIS0.

    Expressioni

    Gene expression databases

    BgeeiQ8CIS0.
    CleanExiMM_CARD11.
    GenevestigatoriQ8CIS0.

    Interactioni

    Subunit structurei

    Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. CARD11 and BCL10 bind to each other by CARD-CARD interaction. Interacts (via PDZ domain) with DPP4 (via cytoplasmic tail) By similarity.By similarity

    Protein-protein interaction databases

    BioGridi224389. 2 interactions.
    DIPiDIP-49692N.
    IntActiQ8CIS0. 4 interactions.
    MINTiMINT-1764942.
    STRINGi10090.ENSMUSP00000082941.

    Structurei

    Secondary structure

    1
    1159
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi24 – 274
    Helixi30 – 367
    Helixi39 – 4810
    Helixi54 – 618
    Turni70 – 734
    Helixi74 – 807
    Helixi84 – 9815
    Helixi100 – 1078

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4I16X-ray1.75A18-110[»]
    ProteinModelPortaliQ8CIS0.
    SMRiQ8CIS0. Positions 21-109, 685-850.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 11093CARDPROSITE-ProRule annotationAdd
    BLAST
    Domaini672 – 76089PDZAdd
    BLAST
    Domaini978 – 1145168Guanylate kinase-likeAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili176 – 449274Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi623 – 6275Poly-Ser

    Sequence similaritiesi

    Contains 1 CARD domain.PROSITE-ProRule annotation
    Contains 1 guanylate kinase-like domain.Curated
    Contains 1 PDZ (DHR) domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG47732.
    GeneTreeiENSGT00530000063108.
    HOGENOMiHOG000111299.
    HOVERGENiHBG099790.
    InParanoidiQ8CIS0.
    KOiK07367.
    OMAiEALPCLY.
    OrthoDBiEOG747PH5.
    PhylomeDBiQ8CIS0.
    TreeFamiTF351139.

    Family and domain databases

    Gene3Di1.10.533.10. 1 hit.
    2.30.42.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR001315. CARD.
    IPR011029. DEATH-like_dom.
    IPR027417. P-loop_NTPase.
    IPR001478. PDZ.
    [Graphical view]
    PfamiPF00619. CARD. 1 hit.
    [Graphical view]
    SMARTiSM00228. PDZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF47986. SSF47986. 1 hit.
    SSF50156. SSF50156. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50209. CARD. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8CIS0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPGGGPAMDD YMETLKDEEE ALWDNVECNR HMLSRYINPA KLTPYLRQCK     50
    VIDEQDEDEV LNAPMLPSKI NRAGRLLDIL HTKGQRGYVV FLESLEFYYP 100
    ELYKLVTGKE PTRRFSTIVV EEGHEGLTHF LMNEVIKLQQ QVKAKDLQRC 150
    ELLAKSRQLE DEKKQLSLIR VELLTFQERY YKMKEERDSY NDELVKVKDD 200
    NYNLAMRYAQ LSEEKNMAVM RSRDLQLEID QLKHRLNKME EECKLERNQS 250
    LKLKNDIENR PRKEQVLELE RENEMLKTKI QELQSIIQAG KRSLPDSDKA 300
    ILDILEHDRK EALEDRQELV NKIYNLQEEV RQAEELRDKY LEEKEDLELK 350
    CSTLGKDCEM YKHRMNTVML QLEEVERERD QAFHSRDEAQ TQYSQCLIEK 400
    DKYRKQIREL EEKNDEMRIE MVRREACIVN LESKLRRLSK DNGSLDQSLP 450
    RHLPATIISQ NLGDTSPRTN GQEADDSSTS EESPEDSKYF LPYHPPRRRM 500
    NLKGIQLQRA KSPISMKQAS EFQALMRTVK GHEEDFTDGS PSSSRSLPVT 550
    SSFSKMQPHR SRSSIMSITA EPPGNDSIVR RCKEDAPHRS TVEEDNDSCG 600
    FDALDLDDEN HERYSFGPPS IHSSSSSHQS EGLDAYDLEQ VNLMLRKFSL 650
    ERPFRPSVTS GGHVRGTGPL VQHTTLNGDG LITQLTLLGG NARGSFIHSV 700
    KPGSLAERAG LREGHQLLLL EGCIRGERQS VPLDACTKEE ARWTIQRCSG 750
    LITLHYKVNH EGYRKLLKEM EDGLITSGDS FYIRLNLNIS SQLDACSMSL 800
    KCDDVVHVLD TMYQDRHEWL CARVDPFTDQ DLDTGTIPSY SRAQQLLLVK 850
    LQRLVHRGNR EEADSAHHTL RSLRNTLQPE EMLSTSDPRV SPRLSRASFF 900
    FGQLLQFVSR SENKYKRMNS NERVRIISGS PLGSLSRSSL DATKLLTEKH 950
    EELDPENELS RNLTLIPYSL VRAFHCERRR PVLFTPTMLA KTLVQKLLNS 1000
    GGAMEFTICK SDIVTRDEFL RKQKTETIIY SREKNPNTFE CIVPANIEAV 1050
    AAKNKHCLLE AGIGCVRDLI KCKVYPIVLL IRVSEKNIKR FRKLLPRPET 1100
    EEEFLRVCRL KEKELEALPC LYATVEAEMW SSVEELLRVL KDKIVEEQRK 1150
    TIWVDEDQL 1159
    Length:1,159
    Mass (Da):134,040
    Last modified:February 26, 2008 - v2
    Checksum:i2ED53905B99DD94E
    GO
    Isoform 2 (identifier: Q8CIS0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         524-528: Missing.

    Show »
    Length:1,154
    Mass (Da):133,468
    Checksum:iCE5FA71361B2EB19
    GO

    Sequence cautioni

    The sequence BAD21384.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti258 – 2581E → Q in AAN10150. (PubMed:12356734)Curated
    Sequence conflicti860 – 8601R → L in BAC29910. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei524 – 5285Missing in isoform 2. 2 PublicationsVSP_031595

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY135367 mRNA. Translation: AAN10150.1.
    AK131134 mRNA. Translation: BAD21384.1. Different initiation.
    BC096592 mRNA. Translation: AAH96592.1.
    AK037968 mRNA. Translation: BAC29910.1.
    CCDSiCCDS51686.1. [Q8CIS0-2]
    RefSeqiNP_780571.2. NM_175362.2. [Q8CIS0-2]
    UniGeneiMm.46187.

    Genome annotation databases

    EnsembliENSMUST00000085786; ENSMUSP00000082941; ENSMUSG00000036526. [Q8CIS0-2]
    GeneIDi108723.
    KEGGimmu:108723.
    UCSCiuc009aik.2. mouse. [Q8CIS0-2]
    uc012egd.1. mouse. [Q8CIS0-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY135367 mRNA. Translation: AAN10150.1 .
    AK131134 mRNA. Translation: BAD21384.1 . Different initiation.
    BC096592 mRNA. Translation: AAH96592.1 .
    AK037968 mRNA. Translation: BAC29910.1 .
    CCDSi CCDS51686.1. [Q8CIS0-2 ]
    RefSeqi NP_780571.2. NM_175362.2. [Q8CIS0-2 ]
    UniGenei Mm.46187.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4I16 X-ray 1.75 A 18-110 [» ]
    ProteinModelPortali Q8CIS0.
    SMRi Q8CIS0. Positions 21-109, 685-850.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 224389. 2 interactions.
    DIPi DIP-49692N.
    IntActi Q8CIS0. 4 interactions.
    MINTi MINT-1764942.
    STRINGi 10090.ENSMUSP00000082941.

    Proteomic databases

    PaxDbi Q8CIS0.
    PRIDEi Q8CIS0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000085786 ; ENSMUSP00000082941 ; ENSMUSG00000036526 . [Q8CIS0-2 ]
    GeneIDi 108723.
    KEGGi mmu:108723.
    UCSCi uc009aik.2. mouse. [Q8CIS0-2 ]
    uc012egd.1. mouse. [Q8CIS0-1 ]

    Organism-specific databases

    CTDi 84433.
    MGIi MGI:1916978. Card11.

    Phylogenomic databases

    eggNOGi NOG47732.
    GeneTreei ENSGT00530000063108.
    HOGENOMi HOG000111299.
    HOVERGENi HBG099790.
    InParanoidi Q8CIS0.
    KOi K07367.
    OMAi EALPCLY.
    OrthoDBi EOG747PH5.
    PhylomeDBi Q8CIS0.
    TreeFami TF351139.

    Enzyme and pathway databases

    Reactomei REACT_199121. Activation of NF-kappaB in B cells.
    REACT_205561. FCERI mediated NF-kB activation.
    REACT_225145. Downstream TCR signaling.

    Miscellaneous databases

    ChiTaRSi CARD11. mouse.
    NextBioi 361261.
    PROi Q8CIS0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8CIS0.
    CleanExi MM_CARD11.
    Genevestigatori Q8CIS0.

    Family and domain databases

    Gene3Di 1.10.533.10. 1 hit.
    2.30.42.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR001315. CARD.
    IPR011029. DEATH-like_dom.
    IPR027417. P-loop_NTPase.
    IPR001478. PDZ.
    [Graphical view ]
    Pfami PF00619. CARD. 1 hit.
    [Graphical view ]
    SMARTi SM00228. PDZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47986. SSF47986. 1 hit.
    SSF50156. SSF50156. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50209. CARD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CARD11 mediates factor-specific activation of NF-kappaB by the T cell receptor complex."
      Pomerantz J.L., Denny E.M., Baltimore D.
      EMBO J. 21:5184-5194(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Thymus.
    2. "Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.
      DNA Res. 11:127-135(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: 129.
      Tissue: Mammary tumor.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 611-1159.
      Strain: C57BL/6J.
      Tissue: Thymus.
    5. Cited for: FUNCTION, PHOSPHORYLATION AT SER-564; SER-649 AND SER-657.

    Entry informationi

    Entry nameiCAR11_MOUSE
    AccessioniPrimary (citable) accession number: Q8CIS0
    Secondary accession number(s): Q4VA14, Q6KAS3, Q8BYV0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Supposed to contain a SH3 domain which is not detected by PROSITE, Pfam or SMART.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3