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Protein

Caspase recruitment domain-containing protein 11

Gene

Card11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10 (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • B cell differentiation Source: MGI
  • B cell proliferation Source: MGI
  • Fc-epsilon receptor signaling pathway Source: Reactome
  • homeostasis of number of cells Source: MGI
  • immunoglobulin production Source: MGI
  • interleukin-2 secretion Source: MGI
  • lymphocyte activation Source: MGI
  • positive regulation of B cell proliferation Source: MGI
  • positive regulation of cytokine production Source: MGI
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  • positive regulation of interleukin-2 biosynthetic process Source: MGI
  • positive regulation of NF-kappaB transcription factor activity Source: MGI
  • positive regulation of T cell activation Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • regulation of apoptotic process Source: InterPro
  • regulation of B cell differentiation Source: MGI
  • regulation of immune response Source: MGI
  • regulation of T cell differentiation Source: MGI
  • signal transduction Source: MGI
  • T cell activation Source: MGI
  • T cell costimulation Source: UniProtKB
  • thymic T cell selection Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase recruitment domain-containing protein 11
Gene namesi
Name:Card11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1916978. Card11.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • immunological synapse Source: MGI
  • membrane raft Source: MGI
  • plasma membrane Source: MGI
  • T cell receptor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003201021 – 1159Caspase recruitment domain-containing protein 11Add BLAST1159

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei448PhosphoserineBy similarity1
Modified residuei466PhosphoserineCombined sources1
Modified residuei512PhosphoserineCombined sources1
Modified residuei540PhosphoserineBy similarity1
Modified residuei564Phosphoserine; by PKC/PRKCB and PKC/PRKCQ1 Publication1
Modified residuei598PhosphoserineBy similarity1
Modified residuei649Phosphoserine; by PKC/PRKCB and PKC/PRKCQ1 Publication1
Modified residuei657Phosphoserine; by PKC/PRKCB and PKC/PRKCQ1 Publication1
Modified residuei891PhosphoserineCombined sources1
Modified residuei930PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-564, Ser-649 and Ser-657 by PRKCB and PRKCQ leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CIS0.
PeptideAtlasiQ8CIS0.
PRIDEiQ8CIS0.

PTM databases

iPTMnetiQ8CIS0.
PhosphoSitePlusiQ8CIS0.
SwissPalmiQ8CIS0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036526.
CleanExiMM_CARD11.
GenevisibleiQ8CIS0. MM.

Interactioni

Subunit structurei

Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. CARD11 and BCL10 bind to each other by CARD-CARD interaction. Interacts (via PDZ domain) with DPP4 (via cytoplasmic tail) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi224389. 1 interactor.
DIPiDIP-49692N.
IntActiQ8CIS0. 4 interactors.
MINTiMINT-1764942.
STRINGi10090.ENSMUSP00000082941.

Structurei

Secondary structure

11159
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 27Combined sources4
Helixi30 – 36Combined sources7
Helixi39 – 48Combined sources10
Helixi54 – 61Combined sources8
Turni70 – 73Combined sources4
Helixi74 – 80Combined sources7
Helixi84 – 98Combined sources15
Helixi100 – 107Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I16X-ray1.75A18-110[»]
ProteinModelPortaliQ8CIS0.
SMRiQ8CIS0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 110CARDPROSITE-ProRule annotationAdd BLAST93
Domaini672 – 760PDZAdd BLAST89
Domaini978 – 1145Guanylate kinase-likeAdd BLAST168

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili176 – 449Sequence analysisAdd BLAST274

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi623 – 627Poly-Ser5

Sequence similaritiesi

Contains 1 CARD domain.PROSITE-ProRule annotation
Contains 1 guanylate kinase-like domain.Curated
Contains 1 PDZ (DHR) domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410ISRJ. Eukaryota.
ENOG41126VR. LUCA.
GeneTreeiENSGT00530000063108.
HOGENOMiHOG000111299.
HOVERGENiHBG099790.
InParanoidiQ8CIS0.
KOiK07367.
OMAiHRMNTVM.
OrthoDBiEOG091G0T58.
PhylomeDBiQ8CIS0.
TreeFamiTF351139.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
2.30.42.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001315. CARD.
IPR033538. CARD11.
IPR011029. DEATH-like_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR14559:SF4. PTHR14559:SF4. 2 hits.
PfamiPF00619. CARD. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CIS0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGGGPAMDD YMETLKDEEE ALWDNVECNR HMLSRYINPA KLTPYLRQCK
60 70 80 90 100
VIDEQDEDEV LNAPMLPSKI NRAGRLLDIL HTKGQRGYVV FLESLEFYYP
110 120 130 140 150
ELYKLVTGKE PTRRFSTIVV EEGHEGLTHF LMNEVIKLQQ QVKAKDLQRC
160 170 180 190 200
ELLAKSRQLE DEKKQLSLIR VELLTFQERY YKMKEERDSY NDELVKVKDD
210 220 230 240 250
NYNLAMRYAQ LSEEKNMAVM RSRDLQLEID QLKHRLNKME EECKLERNQS
260 270 280 290 300
LKLKNDIENR PRKEQVLELE RENEMLKTKI QELQSIIQAG KRSLPDSDKA
310 320 330 340 350
ILDILEHDRK EALEDRQELV NKIYNLQEEV RQAEELRDKY LEEKEDLELK
360 370 380 390 400
CSTLGKDCEM YKHRMNTVML QLEEVERERD QAFHSRDEAQ TQYSQCLIEK
410 420 430 440 450
DKYRKQIREL EEKNDEMRIE MVRREACIVN LESKLRRLSK DNGSLDQSLP
460 470 480 490 500
RHLPATIISQ NLGDTSPRTN GQEADDSSTS EESPEDSKYF LPYHPPRRRM
510 520 530 540 550
NLKGIQLQRA KSPISMKQAS EFQALMRTVK GHEEDFTDGS PSSSRSLPVT
560 570 580 590 600
SSFSKMQPHR SRSSIMSITA EPPGNDSIVR RCKEDAPHRS TVEEDNDSCG
610 620 630 640 650
FDALDLDDEN HERYSFGPPS IHSSSSSHQS EGLDAYDLEQ VNLMLRKFSL
660 670 680 690 700
ERPFRPSVTS GGHVRGTGPL VQHTTLNGDG LITQLTLLGG NARGSFIHSV
710 720 730 740 750
KPGSLAERAG LREGHQLLLL EGCIRGERQS VPLDACTKEE ARWTIQRCSG
760 770 780 790 800
LITLHYKVNH EGYRKLLKEM EDGLITSGDS FYIRLNLNIS SQLDACSMSL
810 820 830 840 850
KCDDVVHVLD TMYQDRHEWL CARVDPFTDQ DLDTGTIPSY SRAQQLLLVK
860 870 880 890 900
LQRLVHRGNR EEADSAHHTL RSLRNTLQPE EMLSTSDPRV SPRLSRASFF
910 920 930 940 950
FGQLLQFVSR SENKYKRMNS NERVRIISGS PLGSLSRSSL DATKLLTEKH
960 970 980 990 1000
EELDPENELS RNLTLIPYSL VRAFHCERRR PVLFTPTMLA KTLVQKLLNS
1010 1020 1030 1040 1050
GGAMEFTICK SDIVTRDEFL RKQKTETIIY SREKNPNTFE CIVPANIEAV
1060 1070 1080 1090 1100
AAKNKHCLLE AGIGCVRDLI KCKVYPIVLL IRVSEKNIKR FRKLLPRPET
1110 1120 1130 1140 1150
EEEFLRVCRL KEKELEALPC LYATVEAEMW SSVEELLRVL KDKIVEEQRK

TIWVDEDQL
Length:1,159
Mass (Da):134,040
Last modified:February 26, 2008 - v2
Checksum:i2ED53905B99DD94E
GO
Isoform 2 (identifier: Q8CIS0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     524-528: Missing.

Show »
Length:1,154
Mass (Da):133,468
Checksum:iCE5FA71361B2EB19
GO

Sequence cautioni

The sequence BAD21384 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti258E → Q in AAN10150 (PubMed:12356734).Curated1
Sequence conflicti860R → L in BAC29910 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_031595524 – 528Missing in isoform 2. 2 Publications5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135367 mRNA. Translation: AAN10150.1.
AK131134 mRNA. Translation: BAD21384.1. Different initiation.
BC096592 mRNA. Translation: AAH96592.1.
AK037968 mRNA. Translation: BAC29910.1.
CCDSiCCDS51686.1. [Q8CIS0-2]
RefSeqiNP_780571.2. NM_175362.2. [Q8CIS0-2]
UniGeneiMm.46187.

Genome annotation databases

EnsembliENSMUST00000085786; ENSMUSP00000082941; ENSMUSG00000036526. [Q8CIS0-2]
GeneIDi108723.
KEGGimmu:108723.
UCSCiuc009aik.2. mouse. [Q8CIS0-2]
uc012egd.1. mouse. [Q8CIS0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135367 mRNA. Translation: AAN10150.1.
AK131134 mRNA. Translation: BAD21384.1. Different initiation.
BC096592 mRNA. Translation: AAH96592.1.
AK037968 mRNA. Translation: BAC29910.1.
CCDSiCCDS51686.1. [Q8CIS0-2]
RefSeqiNP_780571.2. NM_175362.2. [Q8CIS0-2]
UniGeneiMm.46187.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I16X-ray1.75A18-110[»]
ProteinModelPortaliQ8CIS0.
SMRiQ8CIS0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi224389. 1 interactor.
DIPiDIP-49692N.
IntActiQ8CIS0. 4 interactors.
MINTiMINT-1764942.
STRINGi10090.ENSMUSP00000082941.

PTM databases

iPTMnetiQ8CIS0.
PhosphoSitePlusiQ8CIS0.
SwissPalmiQ8CIS0.

Proteomic databases

PaxDbiQ8CIS0.
PeptideAtlasiQ8CIS0.
PRIDEiQ8CIS0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085786; ENSMUSP00000082941; ENSMUSG00000036526. [Q8CIS0-2]
GeneIDi108723.
KEGGimmu:108723.
UCSCiuc009aik.2. mouse. [Q8CIS0-2]
uc012egd.1. mouse. [Q8CIS0-1]

Organism-specific databases

CTDi84433.
MGIiMGI:1916978. Card11.

Phylogenomic databases

eggNOGiENOG410ISRJ. Eukaryota.
ENOG41126VR. LUCA.
GeneTreeiENSGT00530000063108.
HOGENOMiHOG000111299.
HOVERGENiHBG099790.
InParanoidiQ8CIS0.
KOiK07367.
OMAiHRMNTVM.
OrthoDBiEOG091G0T58.
PhylomeDBiQ8CIS0.
TreeFamiTF351139.

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.

Miscellaneous databases

ChiTaRSiCard11. mouse.
PROiQ8CIS0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036526.
CleanExiMM_CARD11.
GenevisibleiQ8CIS0. MM.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
2.30.42.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001315. CARD.
IPR033538. CARD11.
IPR011029. DEATH-like_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR14559:SF4. PTHR14559:SF4. 2 hits.
PfamiPF00619. CARD. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAR11_MOUSE
AccessioniPrimary (citable) accession number: Q8CIS0
Secondary accession number(s): Q4VA14, Q6KAS3, Q8BYV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Supposed to contain a SH3 domain which is not detected by PROSITE, Pfam or SMART.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.