Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transient receptor potential cation channel subfamily M member 6

Gene

Trpm6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential ion channel and kinase. Crucial for magnesium homeostasis. Has an important role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1786ATPBy similarity1
Binding sitei1810ATPBy similarity1
Binding sitei1882ATPBy similarity1
Metal bindingi1915ZincBy similarity1
Active sitei1929Proton acceptorBy similarity1
Binding sitei1931ATPBy similarity1
Binding sitei1939ATPBy similarity1
Metal bindingi1972ZincBy similarity1
Metal bindingi1974ZincBy similarity1
Metal bindingi1978ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1956 – 1962ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

  • metal ion transport Source: MGI
  • protein tetramerization Source: InterPro
  • response to toxic substance Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3295583. TRP channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily M member 6 (EC:2.7.11.1)
Alternative name(s):
Channel kinase 2
Melastatin-related TRP cation channel 6
Gene namesi
Name:Trpm6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2675603. Trpm6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 747CytoplasmicSequence analysisAdd BLAST747
Transmembranei748 – 768HelicalSequence analysisAdd BLAST21
Topological domaini769 – 847ExtracellularSequence analysisAdd BLAST79
Transmembranei848 – 868HelicalSequence analysisAdd BLAST21
Topological domaini869 – 910CytoplasmicSequence analysisAdd BLAST42
Transmembranei911 – 931HelicalSequence analysisAdd BLAST21
Topological domaini932 – 945ExtracellularSequence analysisAdd BLAST14
Transmembranei946 – 966HelicalSequence analysisAdd BLAST21
Topological domaini967 – 978CytoplasmicSequence analysisAdd BLAST12
Transmembranei979 – 999HelicalSequence analysisAdd BLAST21
Topological domaini1000 – 1018ExtracellularSequence analysisAdd BLAST19
Intramembranei1019 – 1039Pore-formingSequence analysisAdd BLAST21
Topological domaini1040 – 1053ExtracellularSequence analysisAdd BLAST14
Transmembranei1054 – 1074HelicalSequence analysisAdd BLAST21
Topological domaini1075 – 2028CytoplasmicSequence analysisAdd BLAST954

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • brush border membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153301 – 2028Transient receptor potential cation channel subfamily M member 6Add BLAST2028

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1857Phosphothreonine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CIR4.
PRIDEiQ8CIR4.

PTM databases

iPTMnetiQ8CIR4.
PhosphoSitePlusiQ8CIR4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024727.
GenevisibleiQ8CIR4. MM.

Interactioni

Subunit structurei

Forms heterodimers with TRPM7. Interacts (via kinase domain) with RACK1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037443.

Structurei

3D structure databases

ProteinModelPortaliQ8CIR4.
SMRiQ8CIR4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1756 – 1986Alpha-type protein kinasePROSITE-ProRule annotationAdd BLAST231

Sequence similaritiesi

In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.Curated
Contains 1 alpha-type protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3614. Eukaryota.
ENOG410XR5B. LUCA.
GeneTreeiENSGT00760000119127.
HOGENOMiHOG000230920.
HOVERGENiHBG055663.
InParanoidiQ8CIR4.
KOiK04981.
OMAiLMECMVH.
OrthoDBiEOG091G0DHL.
PhylomeDBiQ8CIR4.
TreeFamiTF314204.

Family and domain databases

InterProiIPR005821. Ion_trans_dom.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
IPR029597. TRPM6.
IPR032415. TRPM_tetra.
[Graphical view]
PANTHERiPTHR13800:SF15. PTHR13800:SF15. 2 hits.
PfamiPF02816. Alpha_kinase. 1 hit.
PF00520. Ion_trans. 1 hit.
PF16519. TRPM_tetra. 1 hit.
[Graphical view]
SMARTiSM00811. Alpha_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51158. ALPHA_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CIR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVKKSWIEG VFYKRECNKF IPSSKDPHRC TPGCQICHNL VRCYCGRLIE
60 70 80 90 100
EHHGLDRAWN LSVTEGHGDE QWSVEKHTVK SPTDTFGTIN FQDGEHIHHS
110 120 130 140 150
KYIRTSWDTK SDHLLHLMLK EWNMELPKLV ISVHGGLQNF KISSKLKETF
160 170 180 190 200
SQGLVKAAET TGAWIITEGI NSGVSKHVGD ALKAHSSKSL RKIWTVGIPP
210 220 230 240 250
WGVIENQREL VGKDVVCMYQ TLGNPLSKLT TLNCMHSHFI LCDDGTVGMY
260 270 280 290 300
GNEEKLRRNL EKHLSMQKIH TCSRQGVPVV GLVMEGGPNV ILWVWETVKN
310 320 330 340 350
KEPVVVCEGT GRAADLLAFT YKHLEDGGIL RPQVKEELFC LIQNMFNFSL
360 370 380 390 400
RQSKHLFQIL MECMVHKDSI TIFDADSEEH QDLDLAILTA LLKGTSLSIS
410 420 430 440 450
EQLNLAMAWD RMDIAKKHIL TYGQHWKPGA LEQAMLDALV MDRVDFVKLL
460 470 480 490 500
IENGVNLHRF LTIPRLEELY NTKQGPTNKF LRHLVQDVKQ HTLLSSYRIT
510 520 530 540 550
LIDIGLVIEY LIGGAYRSSY TRKSFRILYN NLYRKHKSVS SFAQGLSQHS
560 570 580 590 600
LHQRHSLRNR KESSESTLHS QFFRTAQPYK SKEKPEDSQK SKKKSKERQS
610 620 630 640 650
LSEEPEAAGF IYPYNDLLVW AVLMKRQNMA MFFWQHGEEA TVKAVIASIL
660 670 680 690 700
YRAMAREAKE SNMVDDTSEE LKNYSEQFGQ LALDVLEKAF KQNEPMAMKL
710 720 730 740 750
LTYELKNWSN STCLKLAVSG GLRPFVSHSC TQMLLTDMWM GRLKMRKNSW
760 770 780 790 800
LKIIISILLP PMILTLEFKS KAEMSHVPQS QDFQFTWNYS DQGLSNTKES
810 820 830 840 850
ACVKDYDLER GPDEKPDEPL HLDLRNVPQS LPWTRRVYEF YSAPFVKFWF
860 870 880 890 900
YTMAYLAFLM LFTYTVLVEM QPQPSVHEWL VIIYIFTNAI EKVREICISE
910 920 930 940 950
PSKFKQKVKM WLSEYWNLME TVAIGLFAVG FGLRWGHPPL QTAGRLIYCI
960 970 980 990 1000
DIIFWFSRLM DFFAVNQHAG PYVTMIAKMA ANMFYIVIIM AIVLLSFGVA
1010 1020 1030 1040 1050
RKAILSPKEP PSWRLARDIV FEPYWMMYGE VYASDIDVCS NETSCPPGSF
1060 1070 1080 1090 1100
LTPFLQAVYL FVQYIIMVNL LIACFNNIYL DIKSISNKLW KYNRYRYIMT
1110 1120 1130 1140 1150
YHQKPWLPPP FILLNHLCLL LRGLCCRPAP QDQEEGDGGL KLYLTKDDLK
1160 1170 1180 1190 1200
KLHDFEEQCV EKYFHEKTEG LNCSFEEQIR MTSERVSEMF FQLKEMNEKV
1210 1220 1230 1240 1250
SFIKDSLLSL DSQVGHLQDL SAITVDTLKV LSAVDTLQED EILLANRKHS
1260 1270 1280 1290 1300
TCRKRPHSWT NVICAKVLSD MESCGKKKLQ YYSMPPSLLR SLARSQLPPS
1310 1320 1330 1340 1350
VQRGALVEVT HSKREASHVR EEQEEREMEQ RTTASGISHV RQAHSKYGQF
1360 1370 1380 1390 1400
LLVPSSGKQV PLSLETPPHL FRSSEEAGID GLVLEHIHQS DLTTHLPQQT
1410 1420 1430 1440 1450
PAASHQALVA EHKDQHEAVT QMSDKPAKAE QDLLAFSGTP APMTVTSLPS
1460 1470 1480 1490 1500
RAISMQDEGG YVNWAFSEND ETGVFSFKKK WKTCLASTCN SDSNPGGDYF
1510 1520 1530 1540 1550
LHTGGRSGLD NSRRLAQSCE CPAGPWTQAR RSFWINPLCR DKALIKSHSF
1560 1570 1580 1590 1600
RFHKEEKLRK TWKNNSHSKS LETRSTWLKA KLLTKTRSLS KKKRKTQGLQ
1610 1620 1630 1640 1650
VPVITVNACY QSDQLNAEPG ETNTTEEFSK KWLSVSNFSQ IGLEPYIYQK
1660 1670 1680 1690 1700
MKMKEIKRHT TQASDHLRQP QENRDKTPTW NSGSTSLSRS FLTRSPNEVH
1710 1720 1730 1740 1750
KISTSLKSPQ EPHHHYSAIE RNNLMRLSQT IPFTPIQLFT GEEVTIYKLE
1760 1770 1780 1790 1800
ESSPLTLDKS MSSWSQHGRA AMIQVLSQEE MDGGLRKAMR VISTWSEDDV
1810 1820 1830 1840 1850
LKPGQVFIVK SFLPEVVQTW YKIFQESTVL HLCLREIQQQ RAAQKLIYTF
1860 1870 1880 1890 1900
NQVKPQTIPY TPRFLEVSLV YCHSANQWLT IEKYMTGEFR KYNNNNGDEI
1910 1920 1930 1940 1950
APTNTLEELM LAFSHWTYEY TRGELLVLDL QGVGENLTDP SVIKPEDKQS
1960 1970 1980 1990 2000
RGMVFGPANL GEDAIRSFIA KHRCNSCCGK LRLPDLKRND YSLSRTHCNL
2010 2020
GFGQTIEPTE ELPERDKNRS SLEDHTRL
Length:2,028
Mass (Da):232,888
Last modified:March 1, 2003 - v1
Checksum:i3060C1D70DC9A20E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135644 mRNA. Translation: AAN15217.1.
CCDSiCCDS29691.1.
RefSeqiNP_700466.1. NM_153417.1.
UniGeneiMm.215171.

Genome annotation databases

EnsembliENSMUST00000040489; ENSMUSP00000037443; ENSMUSG00000024727.
GeneIDi225997.
KEGGimmu:225997.
UCSCiuc008gya.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135644 mRNA. Translation: AAN15217.1.
CCDSiCCDS29691.1.
RefSeqiNP_700466.1. NM_153417.1.
UniGeneiMm.215171.

3D structure databases

ProteinModelPortaliQ8CIR4.
SMRiQ8CIR4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037443.

PTM databases

iPTMnetiQ8CIR4.
PhosphoSitePlusiQ8CIR4.

Proteomic databases

PaxDbiQ8CIR4.
PRIDEiQ8CIR4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040489; ENSMUSP00000037443; ENSMUSG00000024727.
GeneIDi225997.
KEGGimmu:225997.
UCSCiuc008gya.1. mouse.

Organism-specific databases

CTDi140803.
MGIiMGI:2675603. Trpm6.

Phylogenomic databases

eggNOGiKOG3614. Eukaryota.
ENOG410XR5B. LUCA.
GeneTreeiENSGT00760000119127.
HOGENOMiHOG000230920.
HOVERGENiHBG055663.
InParanoidiQ8CIR4.
KOiK04981.
OMAiLMECMVH.
OrthoDBiEOG091G0DHL.
PhylomeDBiQ8CIR4.
TreeFamiTF314204.

Enzyme and pathway databases

ReactomeiR-MMU-3295583. TRP channels.

Miscellaneous databases

PROiQ8CIR4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024727.
GenevisibleiQ8CIR4. MM.

Family and domain databases

InterProiIPR005821. Ion_trans_dom.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
IPR029597. TRPM6.
IPR032415. TRPM_tetra.
[Graphical view]
PANTHERiPTHR13800:SF15. PTHR13800:SF15. 2 hits.
PfamiPF02816. Alpha_kinase. 1 hit.
PF00520. Ion_trans. 1 hit.
PF16519. TRPM_tetra. 1 hit.
[Graphical view]
SMARTiSM00811. Alpha_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51158. ALPHA_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPM6_MOUSE
AccessioniPrimary (citable) accession number: Q8CIR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.