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Q8CIR4 (TRPM6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transient receptor potential cation channel subfamily M member 6

EC=2.7.11.1
Alternative name(s):
Channel kinase 2
Melastatin-related TRP cation channel 6
Gene names
Name:Trpm6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2028 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Essential ion channel and kinase. Crucial for magnesium homeostasis. Has an important role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Forms heterodimers with TRPM7. Interacts (via kinase domain) with GNB2L1/RACK1 By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.

In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM6 sub-subfamily. [View classification]

Contains 1 alpha-type protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 20282028Transient receptor potential cation channel subfamily M member 6
PRO_0000215330

Regions

Topological domain1 – 747747Cytoplasmic Potential
Transmembrane748 – 76821Helical; Potential
Topological domain769 – 84779Extracellular Potential
Transmembrane848 – 86821Helical; Potential
Topological domain869 – 91042Cytoplasmic Potential
Transmembrane911 – 93121Helical; Potential
Topological domain932 – 94514Extracellular Potential
Transmembrane946 – 96621Helical; Potential
Topological domain967 – 97812Cytoplasmic Potential
Transmembrane979 – 99921Helical; Potential
Topological domain1000 – 101819Extracellular Potential
Intramembrane1019 – 103921Pore-forming; Potential
Topological domain1040 – 105314Extracellular Potential
Transmembrane1054 – 107421Helical; Potential
Topological domain1075 – 2028954Cytoplasmic Potential
Domain1756 – 1986231Alpha-type protein kinase
Nucleotide binding1956 – 19627ATP By similarity

Sites

Active site19291Proton acceptor By similarity
Metal binding19151Zinc By similarity
Metal binding19721Zinc By similarity
Metal binding19741Zinc By similarity
Metal binding19781Zinc By similarity
Binding site17861ATP By similarity
Binding site18101ATP By similarity
Binding site18821ATP By similarity
Binding site19311ATP By similarity
Binding site19391ATP By similarity

Amino acid modifications

Modified residue18571Phosphothreonine; by autocatalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CIR4 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 3060C1D70DC9A20E

FASTA2,028232,888
        10         20         30         40         50         60 
MQVKKSWIEG VFYKRECNKF IPSSKDPHRC TPGCQICHNL VRCYCGRLIE EHHGLDRAWN 

        70         80         90        100        110        120 
LSVTEGHGDE QWSVEKHTVK SPTDTFGTIN FQDGEHIHHS KYIRTSWDTK SDHLLHLMLK 

       130        140        150        160        170        180 
EWNMELPKLV ISVHGGLQNF KISSKLKETF SQGLVKAAET TGAWIITEGI NSGVSKHVGD 

       190        200        210        220        230        240 
ALKAHSSKSL RKIWTVGIPP WGVIENQREL VGKDVVCMYQ TLGNPLSKLT TLNCMHSHFI 

       250        260        270        280        290        300 
LCDDGTVGMY GNEEKLRRNL EKHLSMQKIH TCSRQGVPVV GLVMEGGPNV ILWVWETVKN 

       310        320        330        340        350        360 
KEPVVVCEGT GRAADLLAFT YKHLEDGGIL RPQVKEELFC LIQNMFNFSL RQSKHLFQIL 

       370        380        390        400        410        420 
MECMVHKDSI TIFDADSEEH QDLDLAILTA LLKGTSLSIS EQLNLAMAWD RMDIAKKHIL 

       430        440        450        460        470        480 
TYGQHWKPGA LEQAMLDALV MDRVDFVKLL IENGVNLHRF LTIPRLEELY NTKQGPTNKF 

       490        500        510        520        530        540 
LRHLVQDVKQ HTLLSSYRIT LIDIGLVIEY LIGGAYRSSY TRKSFRILYN NLYRKHKSVS 

       550        560        570        580        590        600 
SFAQGLSQHS LHQRHSLRNR KESSESTLHS QFFRTAQPYK SKEKPEDSQK SKKKSKERQS 

       610        620        630        640        650        660 
LSEEPEAAGF IYPYNDLLVW AVLMKRQNMA MFFWQHGEEA TVKAVIASIL YRAMAREAKE 

       670        680        690        700        710        720 
SNMVDDTSEE LKNYSEQFGQ LALDVLEKAF KQNEPMAMKL LTYELKNWSN STCLKLAVSG 

       730        740        750        760        770        780 
GLRPFVSHSC TQMLLTDMWM GRLKMRKNSW LKIIISILLP PMILTLEFKS KAEMSHVPQS 

       790        800        810        820        830        840 
QDFQFTWNYS DQGLSNTKES ACVKDYDLER GPDEKPDEPL HLDLRNVPQS LPWTRRVYEF 

       850        860        870        880        890        900 
YSAPFVKFWF YTMAYLAFLM LFTYTVLVEM QPQPSVHEWL VIIYIFTNAI EKVREICISE 

       910        920        930        940        950        960 
PSKFKQKVKM WLSEYWNLME TVAIGLFAVG FGLRWGHPPL QTAGRLIYCI DIIFWFSRLM 

       970        980        990       1000       1010       1020 
DFFAVNQHAG PYVTMIAKMA ANMFYIVIIM AIVLLSFGVA RKAILSPKEP PSWRLARDIV 

      1030       1040       1050       1060       1070       1080 
FEPYWMMYGE VYASDIDVCS NETSCPPGSF LTPFLQAVYL FVQYIIMVNL LIACFNNIYL 

      1090       1100       1110       1120       1130       1140 
DIKSISNKLW KYNRYRYIMT YHQKPWLPPP FILLNHLCLL LRGLCCRPAP QDQEEGDGGL 

      1150       1160       1170       1180       1190       1200 
KLYLTKDDLK KLHDFEEQCV EKYFHEKTEG LNCSFEEQIR MTSERVSEMF FQLKEMNEKV 

      1210       1220       1230       1240       1250       1260 
SFIKDSLLSL DSQVGHLQDL SAITVDTLKV LSAVDTLQED EILLANRKHS TCRKRPHSWT 

      1270       1280       1290       1300       1310       1320 
NVICAKVLSD MESCGKKKLQ YYSMPPSLLR SLARSQLPPS VQRGALVEVT HSKREASHVR 

      1330       1340       1350       1360       1370       1380 
EEQEEREMEQ RTTASGISHV RQAHSKYGQF LLVPSSGKQV PLSLETPPHL FRSSEEAGID 

      1390       1400       1410       1420       1430       1440 
GLVLEHIHQS DLTTHLPQQT PAASHQALVA EHKDQHEAVT QMSDKPAKAE QDLLAFSGTP 

      1450       1460       1470       1480       1490       1500 
APMTVTSLPS RAISMQDEGG YVNWAFSEND ETGVFSFKKK WKTCLASTCN SDSNPGGDYF 

      1510       1520       1530       1540       1550       1560 
LHTGGRSGLD NSRRLAQSCE CPAGPWTQAR RSFWINPLCR DKALIKSHSF RFHKEEKLRK 

      1570       1580       1590       1600       1610       1620 
TWKNNSHSKS LETRSTWLKA KLLTKTRSLS KKKRKTQGLQ VPVITVNACY QSDQLNAEPG 

      1630       1640       1650       1660       1670       1680 
ETNTTEEFSK KWLSVSNFSQ IGLEPYIYQK MKMKEIKRHT TQASDHLRQP QENRDKTPTW 

      1690       1700       1710       1720       1730       1740 
NSGSTSLSRS FLTRSPNEVH KISTSLKSPQ EPHHHYSAIE RNNLMRLSQT IPFTPIQLFT 

      1750       1760       1770       1780       1790       1800 
GEEVTIYKLE ESSPLTLDKS MSSWSQHGRA AMIQVLSQEE MDGGLRKAMR VISTWSEDDV 

      1810       1820       1830       1840       1850       1860 
LKPGQVFIVK SFLPEVVQTW YKIFQESTVL HLCLREIQQQ RAAQKLIYTF NQVKPQTIPY 

      1870       1880       1890       1900       1910       1920 
TPRFLEVSLV YCHSANQWLT IEKYMTGEFR KYNNNNGDEI APTNTLEELM LAFSHWTYEY 

      1930       1940       1950       1960       1970       1980 
TRGELLVLDL QGVGENLTDP SVIKPEDKQS RGMVFGPANL GEDAIRSFIA KHRCNSCCGK 

      1990       2000       2010       2020 
LRLPDLKRND YSLSRTHCNL GFGQTIEPTE ELPERDKNRS SLEDHTRL 

« Hide

References

[1]Ryazanova L.V., Ryazanov A.G.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY135644 mRNA. Translation: AAN15217.1.
CCDSCCDS29691.1.
RefSeqNP_700466.1. NM_153417.1.
UniGeneMm.215171.

3D structure databases

ProteinModelPortalQ8CIR4.
SMRQ8CIR4. Positions 1180-1229, 1713-1992.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000037443.

PTM databases

PhosphoSiteQ8CIR4.

Proteomic databases

PRIDEQ8CIR4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000040489; ENSMUSP00000037443; ENSMUSG00000024727.
GeneID225997.
KEGGmmu:225997.
UCSCuc008gya.1. mouse.

Organism-specific databases

CTD140803.
MGIMGI:2675603. Trpm6.

Phylogenomic databases

eggNOGNOG253824.
GeneTreeENSGT00650000093139.
HOGENOMHOG000230920.
HOVERGENHBG055663.
InParanoidQ8CIR4.
KOK04981.
OMALMECMVH.
OrthoDBEOG7NPFSC.
PhylomeDBQ8CIR4.
TreeFamTF314204.

Gene expression databases

BgeeQ8CIR4.
GenevestigatorQ8CIR4.

Family and domain databases

InterProIPR005821. Ion_trans_dom.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
[Graphical view]
PfamPF02816. Alpha_kinase. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTSM00811. Alpha_kinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS51158. ALPHA_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio377907.
PROQ8CIR4.
SOURCESearch...

Entry information

Entry nameTRPM6_MOUSE
AccessionPrimary (citable) accession number: Q8CIR4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot