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Protein

Dedicator of cytokinesis protein 3

Gene

Dock3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. Its interaction with presenilin proteins as well as its ability to stimulate Tau/MAPT phosphorylation suggest that it may be involved in Alzheimer disease. Ectopic expression in nerve cells decreases the secretion of beta-amyloid APBA1 protein and lowers the rate of cell-substratum adhesion, suggesting that it may affect the function of some small GTPase involved in the regulation of actin cytoskeleton or cell adhesion receptors.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Dedicator of cytokinesis protein 3
Alternative name(s):
Modifier of cell adhesion
Presenilin-binding protein
Short name:
PBP
Gene namesi
Name:Dock3
Synonyms:Moca
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2429763. Dock3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20272027Dedicator of cytokinesis protein 3PRO_0000189989Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1655 – 16551PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8CIQ7.
PaxDbiQ8CIQ7.
PRIDEiQ8CIQ7.

PTM databases

iPTMnetiQ8CIQ7.
PhosphoSiteiQ8CIQ7.

Expressioni

Tissue specificityi

Expressed in brain. Not expressed in heart, liver, kidney, spleen and lung. In brain, it is highly expressed in the cerebral cortex and hippocampus, while it is absent in other tissues, except in spinal cord. In the cerebral cortex, it is found within the intermediate (III and IV) and deep (V and VI) layers, whereas it is weakly expressed in superficial layer I. It is also abundant in the piriform cortex. Within the hippocampus, it is expressed in the pyramidal neurons of the CA1, CA2, and CA3 regions and the dentate gyrus.1 Publication

Gene expression databases

CleanExiMM_DOCK3.

Interactioni

Subunit structurei

Interacts with presenilin proteins PSEN1 and PSEN2. Interacts with CRK.2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047652.

Structurei

3D structure databases

ProteinModelPortaliQ8CIQ7.
SMRiQ8CIQ7. Positions 2-164, 421-600, 1209-1627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 6762SH3PROSITE-ProRule annotationAdd
BLAST
Domaini421 – 598178DHR-1PROSITE-ProRule annotationAdd
BLAST
Domaini1225 – 1632408DHR-2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1967 – 19737SH3-bindingSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1728 – 176538Ser-richAdd
BLAST

Domaini

The DHR-2 domain may mediate some GEF activity.By similarity

Sequence similaritiesi

Belongs to the DOCK family.PROSITE-ProRule annotation
Contains 1 DHR-1 domain.PROSITE-ProRule annotation
Contains 1 DHR-2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain, SH3-binding

Phylogenomic databases

eggNOGiKOG1998. Eukaryota.
ENOG410XQH7. LUCA.
HOGENOMiHOG000006631.
HOVERGENiHBG051389.
InParanoidiQ8CIQ7.
PhylomeDBiQ8CIQ7.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR026800. DOCK3.
IPR010703. DOCK_C.
IPR032376. DOCK_N.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 1 hit.
PTHR23317:SF66. PTHR23317:SF66. 1 hit.
PfamiPF06920. DHR-2. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF16172. DOCK_N. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF50044. SSF50044. 1 hit.
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CIQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWTPTEEEKY GVVICSFRGS VPQGLVLEIG ETVQILEKCE GWYRGVSTKK
60 70 80 90 100
PNVKGLFPAN YIHLKKAIVS NRGQYETVVP LEDSIVTEVT TTLQEWASLW
110 120 130 140 150
KQLYVKHKVD LFYKLRHVMN ELIDLRRQLL SGHLTQDQVR EVKRHITVRL
160 170 180 190 200
DWGNEHLGLD LVPRKDFEVV DSDQISVSDL YKMHLSSRQS VQQSTSQVDT
210 220 230 240 250
MRPRHGETCR MPVPYHFFFS LKSFTYNTIG EDSDVFFSLY DMREGKQISE
260 270 280 290 300
RFLVRLNKNG GPRNPEKIER MCALFTDLSS KDMKRDLYIV AHVIRIGRML
310 320 330 340 350
LNDSKKGPAH LHYRRPYGCA VLSILDVLQS LTELKEEKDF VLKVYTCNNE
360 370 380 390 400
SEWTQIHENI IRKSSTKYSA PSASHGLIIS LQLFRGDMEQ IRRENPMIFN
410 420 430 440 450
RGLAITRKLG FPDVIMPGDI RNDLYLTLEK GDFERGGKSV QKNIEVTMYV
460 470 480 490 500
LYADGEILKD CISLGSGEPN RSSYHSFVLY HSNSPRWGEI IKLPIPIDRF
510 520 530 540 550
RGSHLRFEFR HCSTKDKGEK KLFGFAFSPL MRDDGTTLSD DIHELYVYKC
560 570 580 590 600
DENSTFNNHA LYLGLPCCKE DYNGCPNIPS SLIFQRSKES FFISTQLSST
610 620 630 640 650
KLTQNVDLLA LLKWKAFPDR IMDILGRLRH VSGEEIVKFL QDILDTLFVI
660 670 680 690 700
LDDNTEKYGL LVFQSLVFII NLLRDIKYFH FRPVMDTYIQ KHFAGALAYK
710 720 730 740 750
ELIRCLKWYM DCSAELIRQD HIQEAMRALE YLFKFIVQSR ILYSRATCGM
760 770 780 790 800
EEEQFRSSIQ ELFQSIRFVL SLDSRNSETL LFTQAALLNS FPTIFDELLQ
810 820 830 840 850
MFTVQEVAEF VRGTLGSMPS TVHIGQSMDV VKLQSIARTV DSRLFSFSES
860 870 880 890 900
RRILLPVVLH HIHLHLRQQK ELLICSGILG SIFSIVKTSS LEADVMEEVE
910 920 930 940 950
MMVESLLDVL LQTLLTIMSK SHAQEAVRGH CPVTAEITGE YVSCLLSLLR
960 970 980 990 1000
QMCDTHFQHL LDNFQSKDEL KEFLLKIFCV FRNLMKMSVF PRDWMVMRLL
1010 1020 1030 1040 1050
TSNIIVTTVQ YLSSALHKNF TETDFDFKVW NSYFSLAVLF INQPSLQLEI
1060 1070 1080 1090 1100
ITSAKRKKIL DKYGDMRVMM AYELFSMWQN LGEHKIHFIP GMIGPFLGVT
1110 1120 1130 1140 1150
LVPQPEVRNI MIPIFHDMMD WEQRKNGNFK QVEAELIDKL DSMVSEGKGD
1160 1170 1180 1190 1200
ESYRELFGLL TQLFGPYPSL LEKVEQETWR ETGISFVTSV TRLMERLLDY
1210 1220 1230 1240 1250
RDCMKGEETE NKKVGCTVNL MNFYKSEINK EEMYIRYIHK LCDMHLQAEN
1260 1270 1280 1290 1300
YTEAAFTLLL YCELLQWEDR PLREFLHYPS QTEWQRKEGL CRKIIHYFNK
1310 1320 1330 1340 1350
GKSWEFGIPL CRELACQYES LYDYQSLSWI RKMEASYYDN IIEQQRLEPE
1360 1370 1380 1390 1400
FFRVGFYGRK FPFFLRNKEY VCRGHDYERL EAFQQRMLSE FPQAVAMQHP
1410 1420 1430 1440 1450
NHPDDAILQC DAQYLQIYAV TPIPDYVDVL QMDRVPDRVK SFYRVNNVRK
1460 1470 1480 1490 1500
FRYDRPFHKG PKDKDNEFKS LWIERTTLTL THSLPGISRW FEVERRELVE
1510 1520 1530 1540 1550
VSPLENAIQV VENKNQELRA LISQYQHKQV HGNINLLSMC LNGVIDAAVN
1560 1570 1580 1590 1600
GGIARYQEAF FDKDYITKHP GDAEKISQLK ELMQEQVHVL GVGLAVHEKF
1610 1620 1630 1640 1650
VHPEMRPLHK KLIDQFQMMR ASLYHEFPGL DKLSPACSGT STPRGNVLAS
1660 1670 1680 1690 1700
HSPMSPENIK MTHRHSPMNL MGTGRHSSSS LSSHASSEAG NMMMMGDNSM
1710 1720 1730 1740 1750
GEAPEDLYHH MQLAYHNPRY QGSVTNVSVL SSSQASPSSS SLSSTHSAPS
1760 1770 1780 1790 1800
QMITSAPSST RGSPSLPDKY RHAREMMLLL PTHRDRPSSA MYPAAILENG
1810 1820 1830 1840 1850
QPPNFQRALF QQVVGACKPC SDPNLSMAEK GHYSLHFDAF HHPLGDTPPA
1860 1870 1880 1890 1900
LPARTLRKSP LHPIPASPTS PQSGLDGSNS TLSGSASSGV SSLSESNFGH
1910 1920 1930 1940 1950
SSEAPPRTDT MDSMPSQAWN GDEGLEPPYL PVHYSLSESA VLDAIKSQPC
1960 1970 1980 1990 2000
RSHSAPGCVL PQDPMDPPAL PPKPYHPRLP ALEHDEGMLL REEAERPRGL
2010 2020
HRKASLPPGS VKEEQARLAW EHGRGEQ
Length:2,027
Mass (Da):232,910
Last modified:March 1, 2003 - v1
Checksum:i006DCE0B4583A1CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY145302 mRNA. Translation: AAN12300.1.
UniGeneiMm.150259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY145302 mRNA. Translation: AAN12300.1.
UniGeneiMm.150259.

3D structure databases

ProteinModelPortaliQ8CIQ7.
SMRiQ8CIQ7. Positions 2-164, 421-600, 1209-1627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047652.

PTM databases

iPTMnetiQ8CIQ7.
PhosphoSiteiQ8CIQ7.

Proteomic databases

MaxQBiQ8CIQ7.
PaxDbiQ8CIQ7.
PRIDEiQ8CIQ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:2429763. Dock3.

Phylogenomic databases

eggNOGiKOG1998. Eukaryota.
ENOG410XQH7. LUCA.
HOGENOMiHOG000006631.
HOVERGENiHBG051389.
InParanoidiQ8CIQ7.
PhylomeDBiQ8CIQ7.

Miscellaneous databases

PROiQ8CIQ7.
SOURCEiSearch...

Gene expression databases

CleanExiMM_DOCK3.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR026800. DOCK3.
IPR010703. DOCK_C.
IPR032376. DOCK_N.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 1 hit.
PTHR23317:SF66. PTHR23317:SF66. 1 hit.
PfamiPF06920. DHR-2. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF16172. DOCK_N. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
SSF50044. SSF50044. 1 hit.
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of novel presenilin binding protein."
    Kashiwa A., Yoshida H., Lee S., Paladino T., Liu Y., Chen Q., Dargusch R., Schubert D., Kimura H.
    J. Neurochem. 75:109-116(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH PSEN1 AND CRK.
    Tissue: Brain.
  2. "A novel mechanism for the regulation of amyloid precursor protein metabolism."
    Chen Q., Kimura H., Schubert D.
    J. Cell Biol. 158:79-89(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PSEN2.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Testis.

Entry informationi

Entry nameiDOCK3_MOUSE
AccessioniPrimary (citable) accession number: Q8CIQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.