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Protein

CUGBP Elav-like family member 3

Gene

Celf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

RNA-binding protein involved in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • positive regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: MGI
  • RNA splicing Source: MGI
  • spermatogenesis Source: MGI
  • sperm motility Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CUGBP Elav-like family member 3
Short name:
CELF-3
Alternative name(s):
Bruno-like protein 1
CUG-BP- and ETR-3-like factor 3
ELAV-type RNA-binding protein 1
Short name:
ETR-1
RNA-binding protein BRUNOL-1
Trinucleotide repeat-containing gene 4 protein
Gene namesi
Name:Celf3
Synonyms:Tnrc4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1926034. Celf3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465CUGBP Elav-like family member 3PRO_0000295199Add
BLAST

Proteomic databases

MaxQBiQ8CIN6.
PaxDbiQ8CIN6.
PRIDEiQ8CIN6.

PTM databases

iPTMnetiQ8CIN6.
PhosphoSiteiQ8CIN6.

Expressioni

Gene expression databases

BgeeiQ8CIN6.
CleanExiMM_TNRC4.
ExpressionAtlasiQ8CIN6. baseline and differential.
GenevisibleiQ8CIN6. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8CIN6. 1 interaction.
MINTiMINT-4116444.
STRINGi10090.ENSMUSP00000029784.

Structurei

3D structure databases

ProteinModelPortaliQ8CIN6.
SMRiQ8CIN6. Positions 1-179, 376-456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 8882RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini95 – 17480RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini380 – 45879RRM 3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi351 – 37323Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the CELF/BRUNOL family.Curated
Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0146. Eukaryota.
ENOG410XNTW. LUCA.
GeneTreeiENSGT00560000076837.
HOGENOMiHOG000004754.
HOVERGENiHBG107646.
InParanoidiQ8CIN6.
KOiK13207.
OMAiVWRGEVF.
OrthoDBiEOG7DVDBR.
PhylomeDBiQ8CIN6.
TreeFamiTF314924.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CIN6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKEPDAIKLF VGQIPRHLEE KDLKPIFEQF GRIFELTVIK DKYTGLHKGC
60 70 80 90 100
AFLTYCARDS ALKAQSALHE QKTLPGMNRP IQVKPADSES RGDRKLFVGM
110 120 130 140 150
LGKQQTDEDV RKMFEPFGTI DECTVLRGPD GTSKGCAFVK FQTHAEAQAA
160 170 180 190 200
INTLHSSRTL PGASSSLVVK FADTEKERGL RRMQQVATQL GMFSPIALQF
210 220 230 240 250
GAYSAYTQAL MQQQAALVAA HSAYLSPMAT MAAVQMQHMA AISANGLIAT
260 270 280 290 300
PITPSSGTST PPAIAATPVS AIPAALGVNG YSPVPTQPTG QPAPDALYPN
310 320 330 340 350
GVHPYPAQSP AAPVDPLQQA YAGMQHYTAA YPAAYSLVAP AFPQPPALVA
360 370 380 390 400
QQPPPPPQQQ QQQQQQQQQQ QQQREGPDGC NIFIYHLPQE FTDSEILQMF
410 420 430 440 450
VPFGHVISAK VFVDRATNQS KCFGFVSFDN PASAQAAIQA MNGFQIGMKR
460
LKVQLKRPKD ANRPY
Length:465
Mass (Da):50,520
Last modified:March 1, 2003 - v1
Checksum:i542DF7AC60CCA705
GO
Isoform 2 (identifier: Q8CIN6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     92-92: G → GE
     306-306: P → PDEALSAERSAGGVPIMSQAHSWLVMLSA

Show »
Length:494
Mass (Da):53,545
Checksum:i4B6E858580D4F28E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei92 – 921G → GE in isoform 2. 1 PublicationVSP_026826
Alternative sequencei306 – 3061P → PDEALSAERSAGGVPIMSQA HSWLVMLSA in isoform 2. 1 PublicationVSP_026827

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY165052 mRNA. Translation: AAN73885.1.
BC057553 mRNA. Translation: AAH57553.1.
CCDSiCCDS38536.1. [Q8CIN6-1]
CCDS79972.1. [Q8CIN6-2]
RefSeqiNP_001276542.1. NM_001289613.1. [Q8CIN6-2]
NP_001276545.1. NM_001289616.1.
NP_001276549.1. NM_001289620.1.
NP_766022.1. NM_172434.3. [Q8CIN6-1]
XP_006502386.1. XM_006502323.2. [Q8CIN6-2]
UniGeneiMm.44292.

Genome annotation databases

EnsembliENSMUST00000029784; ENSMUSP00000029784; ENSMUSG00000028137. [Q8CIN6-1]
ENSMUST00000200342; ENSMUSP00000143344; ENSMUSG00000028137. [Q8CIN6-2]
GeneIDi78784.
KEGGimmu:78784.
UCSCiuc008qgl.2. mouse. [Q8CIN6-1]
uc008qgm.2. mouse. [Q8CIN6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY165052 mRNA. Translation: AAN73885.1.
BC057553 mRNA. Translation: AAH57553.1.
CCDSiCCDS38536.1. [Q8CIN6-1]
CCDS79972.1. [Q8CIN6-2]
RefSeqiNP_001276542.1. NM_001289613.1. [Q8CIN6-2]
NP_001276545.1. NM_001289616.1.
NP_001276549.1. NM_001289620.1.
NP_766022.1. NM_172434.3. [Q8CIN6-1]
XP_006502386.1. XM_006502323.2. [Q8CIN6-2]
UniGeneiMm.44292.

3D structure databases

ProteinModelPortaliQ8CIN6.
SMRiQ8CIN6. Positions 1-179, 376-456.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CIN6. 1 interaction.
MINTiMINT-4116444.
STRINGi10090.ENSMUSP00000029784.

PTM databases

iPTMnetiQ8CIN6.
PhosphoSiteiQ8CIN6.

Proteomic databases

MaxQBiQ8CIN6.
PaxDbiQ8CIN6.
PRIDEiQ8CIN6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029784; ENSMUSP00000029784; ENSMUSG00000028137. [Q8CIN6-1]
ENSMUST00000200342; ENSMUSP00000143344; ENSMUSG00000028137. [Q8CIN6-2]
GeneIDi78784.
KEGGimmu:78784.
UCSCiuc008qgl.2. mouse. [Q8CIN6-1]
uc008qgm.2. mouse. [Q8CIN6-2]

Organism-specific databases

CTDi11189.
MGIiMGI:1926034. Celf3.

Phylogenomic databases

eggNOGiKOG0146. Eukaryota.
ENOG410XNTW. LUCA.
GeneTreeiENSGT00560000076837.
HOGENOMiHOG000004754.
HOVERGENiHBG107646.
InParanoidiQ8CIN6.
KOiK13207.
OMAiVWRGEVF.
OrthoDBiEOG7DVDBR.
PhylomeDBiQ8CIN6.
TreeFamiTF314924.

Miscellaneous databases

NextBioi349492.
PROiQ8CIN6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CIN6.
CleanExiMM_TNRC4.
ExpressionAtlasiQ8CIN6. baseline and differential.
GenevisibleiQ8CIN6. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The RNA binding protein CELF3 associates to the DMPK mRNA and is a negative regulator of mRNA stability involved in neuronal differentiation."
    d'Apolito M., Savino M., Grifa A., Quattrone A.
    Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Fetal brain.

Entry informationi

Entry nameiCELF3_MOUSE
AccessioniPrimary (citable) accession number: Q8CIN6
Secondary accession number(s): Q6PFH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 1, 2003
Last modified: January 20, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.