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Q8CIM5 (GPR84_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor 84
Gene names
Name:Gpr84
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed predominantly in hematopoietic tissues. Expressed mainly in the bone marrow with transcripts also detected in spleen, the lymph node, liver and the lung. Ref.1 Ref.3

Induction

By lipopolysaccharide in the monocyte/macrophage cell lines. Ref.3

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396G-protein coupled receptor 84
PRO_0000069590

Regions

Topological domain1 – 2121Extracellular Potential
Transmembrane22 – 4221Helical; Name=1; Potential
Topological domain43 – 5715Cytoplasmic Potential
Transmembrane58 – 7821Helical; Name=2; Potential
Topological domain79 – 9416Extracellular Potential
Transmembrane95 – 11521Helical; Name=3; Potential
Topological domain116 – 13520Cytoplasmic Potential
Transmembrane136 – 15621Helical; Name=4; Potential
Topological domain157 – 18024Extracellular Potential
Transmembrane181 – 20121Helical; Name=5; Potential
Topological domain202 – 320119Cytoplasmic Potential
Transmembrane321 – 34121Helical; Name=6; Potential
Topological domain342 – 35211Extracellular Potential
Transmembrane353 – 37321Helical; Name=7; Potential
Topological domain374 – 39623Cytoplasmic Potential

Amino acid modifications

Modified residue2211Phosphoserine Ref.4
Modified residue2241Phosphoserine Ref.4
Glycosylation31N-linked (GlcNAc...) Potential
Glycosylation81N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict241V → I in AAK01859. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8CIM5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 80F0601CBB4B1B03

FASTA39643,717
        10         20         30         40         50         60 
MWNSSDANFS CYHESVLGYR YFAVIWGVAV AVTGTVGNVL TLLALAIRPK LRTRFNLLIA 

        70         80         90        100        110        120 
NLTLADLLYC TLLQPFSVDT YLHLHWRTGA VFCRIFGLLL FTSNSVSILT LCLIALGRYL 

       130        140        150        160        170        180 
LIAHPKLFPQ VFSAKGIVLA LVGSWVVGVT SFAPLWNVFV LVPVVCTCSF DRMRGRPYTT 

       190        200        210        220        230        240 
ILMGIYFVLG LSSVGVFYCL IHRQVKRAAR ALDQYGLHQA SIRSHQVAGT QEAMPGHFQE 

       250        260        270        280        290        300 
LDSGVASRGP SEGISSEPVS AATTQTLEGD SSEAGGQGIR KAAQQIAERS LPEVHRKPRE 

       310        320        330        340        350        360 
TAGARRATDA PSEFGKVTRM CFAVFLCFAL SYIPFLLLNI LDARGRAPRV VHMVAANLTW 

       370        380        390 
LNSCINPVLY AAMNRQFRHA YGSILKRGPQ SFRRFH 

« Hide

References

« Hide 'large scale' references
[1]"An expressed sequence tag (EST) data mining strategy succeeding in the discovery of new G-protein coupled receptors."
Wittenberger T., Schaller H.C., Hellebrand S.
J. Mol. Biol. 307:799-813(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: C57BL/6.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary tumor.
[3]"Medium-chain fatty acids as ligands for orphan G protein-coupled receptor GPR84."
Wang J., Wu X., Simonavicius N., Tian H., Ling L.
J. Biol. Chem. 281:34457-34464(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-221 AND SER-224, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF272948 mRNA. Translation: AAK01859.1.
BC023249 mRNA. Translation: AAH23249.1.
RefSeqNP_109645.1. NM_030720.1.
UniGeneMm.160369.

3D structure databases

ProteinModelPortalQ8CIM5.
SMRQ8CIM5. Positions 30-388.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000078753.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ8CIM5.

Proteomic databases

PRIDEQ8CIM5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000079824; ENSMUSP00000078753; ENSMUSG00000063234.
GeneID80910.
KEGGmmu:80910.
UCSCuc007xxy.1. mouse.

Organism-specific databases

CTD53831.
MGIMGI:1934129. Gpr84.

Phylogenomic databases

eggNOGNOG267354.
GeneTreeENSGT00650000093442.
HOGENOMHOG000077772.
HOVERGENHBG051820.
InParanoidQ8CIM5.
KOK08421.
OrthoDBEOG7M6D7X.
PhylomeDBQ8CIM5.
TreeFamTF333474.

Gene expression databases

BgeeQ8CIM5.
CleanExMM_GPR84.
GenevestigatorQ8CIM5.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PROSITEPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio350278.
PROQ8CIM5.
SOURCESearch...

Entry information

Entry nameGPR84_MOUSE
AccessionPrimary (citable) accession number: Q8CIM5
Secondary accession number(s): Q99MX9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries