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Protein

E3 ubiquitin-protein ligase RFWD3

Gene

Rfwd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Plays a role in RPA-mediated DNA damage signaling and repair (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri288 – 33245RING-type; degeneratePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RFWD3 (EC:6.3.2.-)
Alternative name(s):
RING finger and WD repeat domain-containing protein 3
RING finger protein 201
Gene namesi
Name:Rfwd3
Synonyms:Rnf201
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2384584. Rfwd3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 774774E3 ubiquitin-protein ligase RFWD3PRO_0000278235Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei59 – 591Phosphoserine; by ATM and ATRBy similarity
Modified residuei75 – 751Phosphoserine; by ATM and ATRBy similarity

Post-translational modificationi

Phosphorylated at Ser-59 and Ser-75 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53 (By similarity). Part of the phosphorylation depends upon RPA2 presence (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8CIK8.
MaxQBiQ8CIK8.
PaxDbiQ8CIK8.
PeptideAtlasiQ8CIK8.
PRIDEiQ8CIK8.

PTM databases

iPTMnetiQ8CIK8.
PhosphoSiteiQ8CIK8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033596.
CleanExiMM_RFWD3.
GenevisibleiQ8CIK8. MM.

Interactioni

Subunit structurei

Interacts with MDM2 and p53/TP53. Binds to the RPA complex via direct interaction with RPA2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043780.

Structurei

3D structure databases

ProteinModelPortaliQ8CIK8.
SMRiQ8CIK8. Positions 283-343, 479-621.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati493 – 53543WD 1Add
BLAST
Repeati536 – 56833WD 2Add
BLAST
Repeati583 – 62846WD 3Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili358 – 40346Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 3 WD repeats.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri288 – 33245RING-type; degeneratePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, WD repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1645. Eukaryota.
ENOG410XPPE. LUCA.
GeneTreeiENSGT00390000008931.
HOGENOMiHOG000154104.
HOVERGENiHBG093895.
InParanoidiQ8CIK8.
KOiK15691.
OMAiILCEFQA.
OrthoDBiEOG091G05D0.
PhylomeDBiQ8CIK8.
TreeFamiTF323359.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CIK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEVAVGQAS DLVPSEMDHE VIYSHLQGPL EGTIEPATPT EVVSNGAPLQ
60 70 80 90 100
PAPAELANSQ GGAHVQPAPA EVVSSQDGLP TLQPLPPASI DLTEEVQPSE
110 120 130 140 150
ENMEVVNPGT SEEPSQGSGA NPTAGAARSV SMNNFISGLQ RLHNMLELLR
160 170 180 190 200
PPPADHSVGP IRTRRRMAPI LRARAGESQR QDNGRYVPHT PLYAYFQVSR
210 220 230 240 250
SQPYPLPAAH DSETRNPPSG TDSDSDGSAE DEEVVVQAEE PEANIPEQGV
260 270 280 290 300
IATDQEATSV TGDDAVPKES PQKPNMLSAM EDEEGETCTI CLEQWTNAGD
310 320 330 340 350
HRISALRCGH LFGFRCISKW LKGQTRKCPQ CNKKAKHSDI VVIYARSLRA
360 370 380 390 400
LDTSEQERMK SDLLNEQMLR KQAELESAQC RLQLQVLIDK CTKLNSRVQD
410 420 430 440 450
LEKFVVRHQN SAAQQCSRSK ALCGKCLSSS QSPGKYSSEK TFTISQTGKC
460 470 480 490 500
RILAYCDGLS CLVASQPSPQ ASFLPGFGVK MLSTANMKSS QYIPMHNKQI
510 520 530 540 550
RGLSFSSQSK GLLLSASLDC TIKLTSLETN TVVQTYNTGR PVWSCCWCLD
560 570 580 590 600
ENNYVYAGLA SGSILIYDLR NTNTYIQELV PQKARCPLVS LSYLPKAAVA
610 620 630 640 650
AFPYGGVLAG TLENASFWEL KSDFSHKPHV LSLEPGGFVD FQTESSTRHC
660 670 680 690 700
LVTYRPDKSH NTVRTVLLEM SYKLDDAGEP VCSCLPVQTF LGGPTCKLLT
710 720 730 740 750
KSAIFQSPEN NGNVLVCTGD EASQSTLLWN AGSGLLLQNL KAGEPVLDIC
760 770
PFETNQNSYL ATLTEKTVHM YKWE
Length:774
Mass (Da):84,312
Last modified:March 1, 2003 - v1
Checksum:iC17DC43CC8422E14
GO

Sequence cautioni

The sequence AAH27246 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631A → T in AAH18533 (PubMed:15489334).Curated
Sequence conflicti275 – 2751N → S in AAH18533 (PubMed:15489334).Curated
Sequence conflicti413 – 4131A → V in AAH18533 (PubMed:15489334).Curated
Sequence conflicti459 – 4591L → M in BAE23381 (PubMed:16141072).Curated
Sequence conflicti724 – 7241Q → H in AAH18533 (PubMed:15489334).Curated
Sequence conflicti733 – 7331S → N in AAH18533 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK137499 mRNA. Translation: BAE23381.1.
AK137698 mRNA. Translation: BAE23467.1.
BC018533 mRNA. Translation: AAH18533.2.
BC023694 mRNA. Translation: AAH23694.1.
BC026603 mRNA. Translation: AAH26603.1.
BC027246 mRNA. Translation: AAH27246.1. Different initiation.
BC096602 mRNA. Translation: AAH96602.1.
CCDSiCCDS22672.1.
RefSeqiNP_666330.2. NM_146218.4.
UniGeneiMm.334816.

Genome annotation databases

EnsembliENSMUST00000038739; ENSMUSP00000043780; ENSMUSG00000033596.
GeneIDi234736.
KEGGimmu:234736.
UCSCiuc009nmb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK137499 mRNA. Translation: BAE23381.1.
AK137698 mRNA. Translation: BAE23467.1.
BC018533 mRNA. Translation: AAH18533.2.
BC023694 mRNA. Translation: AAH23694.1.
BC026603 mRNA. Translation: AAH26603.1.
BC027246 mRNA. Translation: AAH27246.1. Different initiation.
BC096602 mRNA. Translation: AAH96602.1.
CCDSiCCDS22672.1.
RefSeqiNP_666330.2. NM_146218.4.
UniGeneiMm.334816.

3D structure databases

ProteinModelPortaliQ8CIK8.
SMRiQ8CIK8. Positions 283-343, 479-621.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043780.

PTM databases

iPTMnetiQ8CIK8.
PhosphoSiteiQ8CIK8.

Proteomic databases

EPDiQ8CIK8.
MaxQBiQ8CIK8.
PaxDbiQ8CIK8.
PeptideAtlasiQ8CIK8.
PRIDEiQ8CIK8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038739; ENSMUSP00000043780; ENSMUSG00000033596.
GeneIDi234736.
KEGGimmu:234736.
UCSCiuc009nmb.1. mouse.

Organism-specific databases

CTDi55159.
MGIiMGI:2384584. Rfwd3.

Phylogenomic databases

eggNOGiKOG1645. Eukaryota.
ENOG410XPPE. LUCA.
GeneTreeiENSGT00390000008931.
HOGENOMiHOG000154104.
HOVERGENiHBG093895.
InParanoidiQ8CIK8.
KOiK15691.
OMAiILCEFQA.
OrthoDBiEOG091G05D0.
PhylomeDBiQ8CIK8.
TreeFamiTF323359.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ8CIK8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033596.
CleanExiMM_RFWD3.
GenevisibleiQ8CIK8. MM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFWD3_MOUSE
AccessioniPrimary (citable) accession number: Q8CIK8
Secondary accession number(s): Q3UV90
, Q6PJA8, Q8R2T9, Q8R351
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.