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Q8CII9

- CRLF2_MOUSE

UniProt

Q8CII9 - CRLF2_MOUSE

Protein

Cytokine receptor-like factor 2

Gene

Crlf2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (07 Nov 2003)
      Previous versions | rss
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    Functioni

    Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2. Implicated in the development of the hematopoietic system.1 Publication

    GO - Biological processi

    1. immunoglobulin secretion involved in immune response Source: MGI
    2. inflammatory response Source: MGI
    3. T-helper 2 cell cytokine production Source: MGI

    Keywords - Molecular functioni

    Receptor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytokine receptor-like factor 2
    Alternative name(s):
    Cytokine receptor-like molecule 2
    Short name:
    CRLM-2
    Thymic stromal lymphopoietin protein receptor
    Short name:
    TSLP receptor
    Type I cytokine receptor delta 1
    Gene namesi
    Name:Crlf2
    Synonyms:Crlm2, Tpte2, Tslpr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1889506. Crlf2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 359340Cytokine receptor-like factor 2PRO_0000011042Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi53 – 531N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi68 ↔ 82By similarity
    Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ8CII9.
    PRIDEiQ8CII9.

    PTM databases

    PhosphoSiteiQ8CII9.

    Expressioni

    Tissue specificityi

    High level of expression in liver, lung and testis. Also expressed in heart, brain, spleen, thymus and bone marrow. Highly expressed in progenitors and myeloid cells. Isoform 2 is expressed in primary hemotopoietic cells.

    Inductioni

    Up-regulated in the myeloid 32D cell line by granulocyte colony-stimulating factor (G-CSF).

    Gene expression databases

    BgeeiQ8CII9.
    CleanExiMM_CRLF2.
    GenevestigatoriQ8CII9.

    Interactioni

    Subunit structurei

    Heterodimer of CRLF2 and IL7R.

    Protein-protein interaction databases

    BioGridi208364. 1 interaction.
    DIPiDIP-59471N.

    Structurei

    Secondary structure

    1
    359
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi30 – 345
    Turni35 – 373
    Beta strandi38 – 425
    Beta strandi55 – 606
    Beta strandi69 – 735
    Beta strandi79 – 857
    Beta strandi88 – 903
    Beta strandi92 – 976
    Beta strandi103 – 1097
    Helixi111 – 1144
    Beta strandi124 – 1274
    Beta strandi133 – 1364
    Beta strandi141 – 1433
    Beta strandi145 – 1539
    Beta strandi162 – 1676
    Beta strandi170 – 1734
    Beta strandi180 – 19011
    Helixi192 – 1954
    Beta strandi208 – 2147
    Helixi216 – 2183

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4NN5X-ray1.90C20-222[»]
    4NN6X-ray2.54C20-222[»]
    4NN7X-ray3.78C20-222[»]
    ProteinModelPortaliQ8CII9.
    SMRiQ8CII9. Positions 28-206.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 232213ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini254 – 359106CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei233 – 25321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini119 – 21395Fibronectin type-IIIPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi201 – 2055WSXWS motif
    Motifi262 – 2709Box 1 motif

    Domaini

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

    Sequence similaritiesi

    Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG74051.
    GeneTreeiENSGT00510000049974.
    HOGENOMiHOG000185192.
    HOVERGENiHBG051120.
    KOiK05078.
    OrthoDBiEOG71K63B.
    PhylomeDBiQ8CII9.
    TreeFamiTF342693.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR003961. Fibronectin_type3.
    IPR013783. Ig-like_fold.
    [Graphical view]
    PfamiPF00041. fn3. 1 hit.
    [Graphical view]
    SMARTiSM00060. FN3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 2 hits.
    PROSITEiPS50853. FN3. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: Q8CII9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAWALAVILL PRLLAAAAAA AAVTSRGDVT VVCHDLETVE VTWGSGPDHH    50
    GANLSLEFRY GTGALQPCPR YFLSGAGVTS GCILPAARAG LLELALRDGG 100
    GAMVFKARQR ASAWLKPRPP WNVTLLWTPD GDVTVSWPAH SYLGLDYEVQ 150
    HRESNDDEDA WQTTSGPCCD LTVGGLDPAR CYDFRVRASP RAAHYGLEAQ 200
    PSEWTAVTRL SGAASAASCT ASPAPSPALA PPLLPLGCGL AALLTLSLLL 250
    AALRLRRVKD ALLPCVPDPS GSFPGLFEKH HGNFQAWIAD AQATAPPART 300
    EEEDDLIHPK AKRVEPEDGT SLCTVPRPPS FEPRGPGGGA MVSVGGATFM 350
    VGDSGYMTL 359
    Length:359
    Mass (Da):37,762
    Last modified:November 7, 2003 - v2
    Checksum:iF9C521C54B4AC9DD
    GO
    Isoform 2 (identifier: Q8CII9-2) [UniParc]FASTAAdd to Basket

    Also known as: Soluble CRLM-2

    The sequence of this isoform differs from the canonical sequence as follows:
         217-217: A → G
         218-359: Missing.

    Show »
    Length:217
    Mass (Da):23,086
    Checksum:iD4382C3273C1B8CA
    GO
    Isoform 3 (identifier: Q8CII9-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         217-217: A → AGDPCAAHLPPL

    Note: No experimental confirmation available.

    Show »
    Length:370
    Mass (Da):38,834
    Checksum:i1063D4172A8BE320
    GO
    Isoform 4 (identifier: Q8CII9-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-233: Missing.
         234-234: L → M

    Note: No experimental confirmation available.

    Show »
    Length:126
    Mass (Da):13,236
    Checksum:iBD387A6F9B4CEDF7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti15 – 151A → T in BAA92159. (PubMed:10733486)Curated
    Sequence conflicti51 – 511G → S in AAF82189. (PubMed:10881176)Curated
    Sequence conflicti51 – 511G → S in AAH23788. (PubMed:15489334)Curated
    Sequence conflicti87 – 871A → G in AAF81676. (PubMed:10974032)Curated
    Sequence conflicti179 – 1791A → V in BAA92684. (PubMed:10872831)Curated
    Sequence conflicti179 – 1791A → V in AAF81676. (PubMed:10974032)Curated
    Sequence conflicti309 – 3091P → T in BAA92684. (PubMed:10872831)Curated
    Sequence conflicti309 – 3091P → T in AAF81676. (PubMed:10974032)Curated
    Sequence conflicti309 – 3091P → T in BAB26827. (PubMed:16141072)Curated
    Sequence conflicti355 – 3551G → R in BAB26827. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 233233Missing in isoform 4. 1 PublicationVSP_018990Add
    BLAST
    Alternative sequencei217 – 2171A → G in isoform 2. 1 PublicationVSP_008788
    Alternative sequencei217 – 2171A → AGDPCAAHLPPL in isoform 3. 1 PublicationVSP_008790
    Alternative sequencei218 – 359142Missing in isoform 2. 1 PublicationVSP_008789Add
    BLAST
    Alternative sequencei234 – 2341L → M in isoform 4. 1 PublicationVSP_018991

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB039945 mRNA. Translation: BAA92684.1.
    AB031333 mRNA. Translation: BAA92159.1.
    AF232936 mRNA. Translation: AAF81676.1.
    AF201963 mRNA. Translation: AAF82189.1.
    AK010291 mRNA. Translation: BAB26827.2.
    BC023788 mRNA. Translation: AAH23788.1.
    CCDSiCCDS19521.1. [Q8CII9-1]
    PIRiJC7280.
    RefSeqiNP_001158207.1. NM_001164735.1.
    NP_057924.3. NM_016715.4.
    UniGeneiMm.35771.

    Genome annotation databases

    EnsembliENSMUST00000044579; ENSMUSP00000036326; ENSMUSG00000033467.
    GeneIDi57914.
    KEGGimmu:57914.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB039945 mRNA. Translation: BAA92684.1 .
    AB031333 mRNA. Translation: BAA92159.1 .
    AF232936 mRNA. Translation: AAF81676.1 .
    AF201963 mRNA. Translation: AAF82189.1 .
    AK010291 mRNA. Translation: BAB26827.2 .
    BC023788 mRNA. Translation: AAH23788.1 .
    CCDSi CCDS19521.1. [Q8CII9-1 ]
    PIRi JC7280.
    RefSeqi NP_001158207.1. NM_001164735.1.
    NP_057924.3. NM_016715.4.
    UniGenei Mm.35771.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4NN5 X-ray 1.90 C 20-222 [» ]
    4NN6 X-ray 2.54 C 20-222 [» ]
    4NN7 X-ray 3.78 C 20-222 [» ]
    ProteinModelPortali Q8CII9.
    SMRi Q8CII9. Positions 28-206.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208364. 1 interaction.
    DIPi DIP-59471N.

    PTM databases

    PhosphoSitei Q8CII9.

    Proteomic databases

    PaxDbi Q8CII9.
    PRIDEi Q8CII9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000044579 ; ENSMUSP00000036326 ; ENSMUSG00000033467 .
    GeneIDi 57914.
    KEGGi mmu:57914.

    Organism-specific databases

    CTDi 64109.
    MGIi MGI:1889506. Crlf2.

    Phylogenomic databases

    eggNOGi NOG74051.
    GeneTreei ENSGT00510000049974.
    HOGENOMi HOG000185192.
    HOVERGENi HBG051120.
    KOi K05078.
    OrthoDBi EOG71K63B.
    PhylomeDBi Q8CII9.
    TreeFami TF342693.

    Miscellaneous databases

    NextBioi 314093.
    PROi Q8CII9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8CII9.
    CleanExi MM_CRLF2.
    Genevestigatori Q8CII9.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR003961. Fibronectin_type3.
    IPR013783. Ig-like_fold.
    [Graphical view ]
    Pfami PF00041. fn3. 1 hit.
    [Graphical view ]
    SMARTi SM00060. FN3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 2 hits.
    PROSITEi PS50853. FN3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of CRLM-2, a novel type I cytokine receptor preferentially expressed in hematopoietic cells."
      Hiroyama T., Iwama A., Morita Y., Nakamura Y., Shibuya A., Nakauchi H.
      Biochem. Biophys. Res. Commun. 272:224-229(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Tissue: Embryo.
    2. "Molecular cloning of a novel type I cytokine receptor similar to the common gamma chain."
      Fujio K., Nosaka T., Kojima T., Kawashima T., Yahata T., Copeland N.G., Gilbert D.J., Jenkins N.A., Yamamoto K., Nishimura T., Kitamura T.
      Blood 95:2204-2210(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING.
      Tissue: Lymphocyte.
    3. "Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor. Formation Of a functional heteromeric complex requires interleukin 7 receptor."
      Park L.S., Martin U., Garka K., Gliniak B., Di Santo J.P., Muller W., Largaespada D.A., Copeland N.G., Jenkins N.A., Farr A.G., Ziegler S.F., Morrissey P.J., Paxton R., Sims J.E.
      J. Exp. Med. 192:659-670(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION AS A RECEPTOR FOR TSLP.
      Strain: C57BL/6.
      Tissue: Lymphocyte.
    4. "Cloning of a receptor subunit required for signaling by thymic stromal lymphopoietin."
      Pandey A., Ozaki K., Baumann H., Levin S.D., Puel A., Farr A.G., Ziegler S.F., Leonard W.J., Lodish H.F.
      Nat. Immunol. 1:59-64(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    5. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
      Strain: C57BL/6J.
      Tissue: Embryonic stem cell.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiCRLF2_MOUSE
    AccessioniPrimary (citable) accession number: Q8CII9
    Secondary accession number(s): Q9CRJ6
    , Q9JIE7, Q9JIQ7, Q9JJH8, Q9JMD5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 7, 2003
    Last sequence update: November 7, 2003
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3