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Q8CII9

- CRLF2_MOUSE

UniProt

Q8CII9 - CRLF2_MOUSE

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Protein

Cytokine receptor-like factor 2

Gene

Crlf2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2. Implicated in the development of the hematopoietic system.1 Publication

GO - Biological processi

  1. immunoglobulin secretion involved in immune response Source: MGI
  2. inflammatory response Source: MGI
  3. T-helper 2 cell cytokine production Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Cytokine receptor-like factor 2
Alternative name(s):
Cytokine receptor-like molecule 2
Short name:
CRLM-2
Thymic stromal lymphopoietin protein receptor
Short name:
TSLP receptor
Type I cytokine receptor delta 1
Gene namesi
Name:Crlf2
Synonyms:Crlm2, Tpte2, Tslpr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1889506. Crlf2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 359340Cytokine receptor-like factor 2PRO_0000011042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi53 – 531N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi68 ↔ 82By similarity
Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8CII9.
PRIDEiQ8CII9.

PTM databases

PhosphoSiteiQ8CII9.

Expressioni

Tissue specificityi

High level of expression in liver, lung and testis. Also expressed in heart, brain, spleen, thymus and bone marrow. Highly expressed in progenitors and myeloid cells. Isoform 2 is expressed in primary hemotopoietic cells.

Inductioni

Up-regulated in the myeloid 32D cell line by granulocyte colony-stimulating factor (G-CSF).

Gene expression databases

BgeeiQ8CII9.
CleanExiMM_CRLF2.
GenevestigatoriQ8CII9.

Interactioni

Subunit structurei

Heterodimer of CRLF2 and IL7R.

Protein-protein interaction databases

BioGridi208364. 1 interaction.
DIPiDIP-59471N.

Structurei

Secondary structure

1
359
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 345
Turni35 – 373
Beta strandi38 – 425
Beta strandi55 – 606
Beta strandi69 – 735
Beta strandi79 – 857
Beta strandi88 – 903
Beta strandi92 – 976
Beta strandi103 – 1097
Helixi111 – 1144
Beta strandi124 – 1274
Beta strandi133 – 1364
Beta strandi141 – 1433
Beta strandi145 – 1539
Beta strandi162 – 1676
Beta strandi170 – 1734
Beta strandi180 – 19011
Helixi192 – 1954
Beta strandi208 – 2147
Helixi216 – 2183

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4NN5X-ray1.90C20-222[»]
4NN6X-ray2.54C20-222[»]
4NN7X-ray3.78C20-222[»]
ProteinModelPortaliQ8CII9.
SMRiQ8CII9. Positions 28-206.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 232213ExtracellularSequence AnalysisAdd
BLAST
Topological domaini254 – 359106CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei233 – 25321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini119 – 21395Fibronectin type-IIIPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi201 – 2055WSXWS motif
Motifi262 – 2709Box 1 motif

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG74051.
GeneTreeiENSGT00510000049974.
HOGENOMiHOG000185192.
HOVERGENiHBG051120.
InParanoidiQ8CII9.
KOiK05078.
OrthoDBiEOG71K63B.
PhylomeDBiQ8CII9.
TreeFamiTF342693.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q8CII9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAWALAVILL PRLLAAAAAA AAVTSRGDVT VVCHDLETVE VTWGSGPDHH
60 70 80 90 100
GANLSLEFRY GTGALQPCPR YFLSGAGVTS GCILPAARAG LLELALRDGG
110 120 130 140 150
GAMVFKARQR ASAWLKPRPP WNVTLLWTPD GDVTVSWPAH SYLGLDYEVQ
160 170 180 190 200
HRESNDDEDA WQTTSGPCCD LTVGGLDPAR CYDFRVRASP RAAHYGLEAQ
210 220 230 240 250
PSEWTAVTRL SGAASAASCT ASPAPSPALA PPLLPLGCGL AALLTLSLLL
260 270 280 290 300
AALRLRRVKD ALLPCVPDPS GSFPGLFEKH HGNFQAWIAD AQATAPPART
310 320 330 340 350
EEEDDLIHPK AKRVEPEDGT SLCTVPRPPS FEPRGPGGGA MVSVGGATFM

VGDSGYMTL
Length:359
Mass (Da):37,762
Last modified:November 7, 2003 - v2
Checksum:iF9C521C54B4AC9DD
GO
Isoform 2 (identifier: Q8CII9-2) [UniParc]FASTAAdd to Basket

Also known as: Soluble CRLM-2

The sequence of this isoform differs from the canonical sequence as follows:
     217-217: A → G
     218-359: Missing.

Show »
Length:217
Mass (Da):23,086
Checksum:iD4382C3273C1B8CA
GO
Isoform 3 (identifier: Q8CII9-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-217: A → AGDPCAAHLPPL

Note: No experimental confirmation available.

Show »
Length:370
Mass (Da):38,834
Checksum:i1063D4172A8BE320
GO
Isoform 4 (identifier: Q8CII9-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-233: Missing.
     234-234: L → M

Note: No experimental confirmation available.

Show »
Length:126
Mass (Da):13,236
Checksum:iBD387A6F9B4CEDF7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151A → T in BAA92159. (PubMed:10733486)Curated
Sequence conflicti51 – 511G → S in AAF82189. (PubMed:10881176)Curated
Sequence conflicti51 – 511G → S in AAH23788. (PubMed:15489334)Curated
Sequence conflicti87 – 871A → G in AAF81676. (PubMed:10974032)Curated
Sequence conflicti179 – 1791A → V in BAA92684. (PubMed:10872831)Curated
Sequence conflicti179 – 1791A → V in AAF81676. (PubMed:10974032)Curated
Sequence conflicti309 – 3091P → T in BAA92684. (PubMed:10872831)Curated
Sequence conflicti309 – 3091P → T in AAF81676. (PubMed:10974032)Curated
Sequence conflicti309 – 3091P → T in BAB26827. (PubMed:16141072)Curated
Sequence conflicti355 – 3551G → R in BAB26827. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 233233Missing in isoform 4. 1 PublicationVSP_018990Add
BLAST
Alternative sequencei217 – 2171A → G in isoform 2. 1 PublicationVSP_008788
Alternative sequencei217 – 2171A → AGDPCAAHLPPL in isoform 3. 1 PublicationVSP_008790
Alternative sequencei218 – 359142Missing in isoform 2. 1 PublicationVSP_008789Add
BLAST
Alternative sequencei234 – 2341L → M in isoform 4. 1 PublicationVSP_018991

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB039945 mRNA. Translation: BAA92684.1.
AB031333 mRNA. Translation: BAA92159.1.
AF232936 mRNA. Translation: AAF81676.1.
AF201963 mRNA. Translation: AAF82189.1.
AK010291 mRNA. Translation: BAB26827.2.
BC023788 mRNA. Translation: AAH23788.1.
CCDSiCCDS19521.1. [Q8CII9-1]
PIRiJC7280.
RefSeqiNP_001158207.1. NM_001164735.1.
NP_057924.3. NM_016715.4.
UniGeneiMm.35771.

Genome annotation databases

EnsembliENSMUST00000044579; ENSMUSP00000036326; ENSMUSG00000033467.
GeneIDi57914.
KEGGimmu:57914.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB039945 mRNA. Translation: BAA92684.1 .
AB031333 mRNA. Translation: BAA92159.1 .
AF232936 mRNA. Translation: AAF81676.1 .
AF201963 mRNA. Translation: AAF82189.1 .
AK010291 mRNA. Translation: BAB26827.2 .
BC023788 mRNA. Translation: AAH23788.1 .
CCDSi CCDS19521.1. [Q8CII9-1 ]
PIRi JC7280.
RefSeqi NP_001158207.1. NM_001164735.1.
NP_057924.3. NM_016715.4.
UniGenei Mm.35771.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4NN5 X-ray 1.90 C 20-222 [» ]
4NN6 X-ray 2.54 C 20-222 [» ]
4NN7 X-ray 3.78 C 20-222 [» ]
ProteinModelPortali Q8CII9.
SMRi Q8CII9. Positions 28-206.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 208364. 1 interaction.
DIPi DIP-59471N.

PTM databases

PhosphoSitei Q8CII9.

Proteomic databases

PaxDbi Q8CII9.
PRIDEi Q8CII9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000044579 ; ENSMUSP00000036326 ; ENSMUSG00000033467 .
GeneIDi 57914.
KEGGi mmu:57914.

Organism-specific databases

CTDi 64109.
MGIi MGI:1889506. Crlf2.

Phylogenomic databases

eggNOGi NOG74051.
GeneTreei ENSGT00510000049974.
HOGENOMi HOG000185192.
HOVERGENi HBG051120.
InParanoidi Q8CII9.
KOi K05078.
OrthoDBi EOG71K63B.
PhylomeDBi Q8CII9.
TreeFami TF342693.

Miscellaneous databases

NextBioi 314093.
PROi Q8CII9.
SOURCEi Search...

Gene expression databases

Bgeei Q8CII9.
CleanExi MM_CRLF2.
Genevestigatori Q8CII9.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
InterProi IPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
[Graphical view ]
Pfami PF00041. fn3. 1 hit.
[Graphical view ]
SMARTi SM00060. FN3. 1 hit.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 2 hits.
PROSITEi PS50853. FN3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of CRLM-2, a novel type I cytokine receptor preferentially expressed in hematopoietic cells."
    Hiroyama T., Iwama A., Morita Y., Nakamura Y., Shibuya A., Nakauchi H.
    Biochem. Biophys. Res. Commun. 272:224-229(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Embryo.
  2. "Molecular cloning of a novel type I cytokine receptor similar to the common gamma chain."
    Fujio K., Nosaka T., Kojima T., Kawashima T., Yahata T., Copeland N.G., Gilbert D.J., Jenkins N.A., Yamamoto K., Nishimura T., Kitamura T.
    Blood 95:2204-2210(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING.
    Tissue: Lymphocyte.
  3. "Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor. Formation Of a functional heteromeric complex requires interleukin 7 receptor."
    Park L.S., Martin U., Garka K., Gliniak B., Di Santo J.P., Muller W., Largaespada D.A., Copeland N.G., Jenkins N.A., Farr A.G., Ziegler S.F., Morrissey P.J., Paxton R., Sims J.E.
    J. Exp. Med. 192:659-670(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION AS A RECEPTOR FOR TSLP.
    Strain: C57BL/6.
    Tissue: Lymphocyte.
  4. "Cloning of a receptor subunit required for signaling by thymic stromal lymphopoietin."
    Pandey A., Ozaki K., Baumann H., Levin S.D., Puel A., Farr A.G., Ziegler S.F., Leonard W.J., Lodish H.F.
    Nat. Immunol. 1:59-64(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    Strain: C57BL/6J.
    Tissue: Embryonic stem cell.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary gland.

Entry informationi

Entry nameiCRLF2_MOUSE
AccessioniPrimary (citable) accession number: Q8CII9
Secondary accession number(s): Q9CRJ6
, Q9JIE7, Q9JIQ7, Q9JJH8, Q9JMD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 7, 2003
Last modified: October 29, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3