Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8CII9 (CRLF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytokine receptor-like factor 2
Alternative name(s):
Cytokine receptor-like molecule 2
Short name=CRLM-2
Thymic stromal lymphopoietin protein receptor
Short name=TSLP receptor
Type I cytokine receptor delta 1
Gene names
Name:Crlf2
Synonyms:Crlm2, Tpte2, Tslpr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2. Implicated in the development of the hematopoietic system. Ref.3

Subunit structure

Heterodimer of CRLF2 and IL7R.

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Isoform 3: Cell membrane; Single-pass type I membrane protein.

Isoform 2: Secreted.

Tissue specificity

High level of expression in liver, lung and testis. Also expressed in heart, brain, spleen, thymus and bone marrow. Highly expressed in progenitors and myeloid cells. Isoform 2 is expressed in primary hemotopoietic cells.

Induction

Up-regulated in the myeloid 32D cell line by granulocyte colony-stimulating factor (G-CSF).

Domain

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 5 subfamily.

Contains 1 fibronectin type-III domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q8CII9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8CII9-2)

Also known as: Soluble CRLM-2;

The sequence of this isoform differs from the canonical sequence as follows:
     217-217: A → G
     218-359: Missing.
Isoform 3 (identifier: Q8CII9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     217-217: A → AGDPCAAHLPPL
Note: No experimental confirmation available.
Isoform 4 (identifier: Q8CII9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-233: Missing.
     234-234: L → M
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 359340Cytokine receptor-like factor 2
PRO_0000011042

Regions

Topological domain20 – 232213Extracellular Potential
Transmembrane233 – 25321Helical; Potential
Topological domain254 – 359106Cytoplasmic Potential
Domain119 – 21395Fibronectin type-III
Motif201 – 2055WSXWS motif
Motif262 – 2709Box 1 motif

Amino acid modifications

Glycosylation531N-linked (GlcNAc...) Potential
Glycosylation1221N-linked (GlcNAc...) Potential
Disulfide bond68 ↔ 82 By similarity

Natural variations

Alternative sequence1 – 233233Missing in isoform 4.
VSP_018990
Alternative sequence2171A → G in isoform 2.
VSP_008788
Alternative sequence2171A → AGDPCAAHLPPL in isoform 3.
VSP_008790
Alternative sequence218 – 359142Missing in isoform 2.
VSP_008789
Alternative sequence2341L → M in isoform 4.
VSP_018991

Experimental info

Sequence conflict151A → T in BAA92159. Ref.2
Sequence conflict511G → S in AAF82189. Ref.4
Sequence conflict511G → S in AAH23788. Ref.6
Sequence conflict871A → G in AAF81676. Ref.3
Sequence conflict1791A → V in BAA92684. Ref.1
Sequence conflict1791A → V in AAF81676. Ref.3
Sequence conflict3091P → T in BAA92684. Ref.1
Sequence conflict3091P → T in AAF81676. Ref.3
Sequence conflict3091P → T in BAB26827. Ref.5
Sequence conflict3551G → R in BAB26827. Ref.5

Secondary structure

....................................... 359
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 7, 2003. Version 2.
Checksum: F9C521C54B4AC9DD

FASTA35937,762
        10         20         30         40         50         60 
MAWALAVILL PRLLAAAAAA AAVTSRGDVT VVCHDLETVE VTWGSGPDHH GANLSLEFRY 

        70         80         90        100        110        120 
GTGALQPCPR YFLSGAGVTS GCILPAARAG LLELALRDGG GAMVFKARQR ASAWLKPRPP 

       130        140        150        160        170        180 
WNVTLLWTPD GDVTVSWPAH SYLGLDYEVQ HRESNDDEDA WQTTSGPCCD LTVGGLDPAR 

       190        200        210        220        230        240 
CYDFRVRASP RAAHYGLEAQ PSEWTAVTRL SGAASAASCT ASPAPSPALA PPLLPLGCGL 

       250        260        270        280        290        300 
AALLTLSLLL AALRLRRVKD ALLPCVPDPS GSFPGLFEKH HGNFQAWIAD AQATAPPART 

       310        320        330        340        350 
EEEDDLIHPK AKRVEPEDGT SLCTVPRPPS FEPRGPGGGA MVSVGGATFM VGDSGYMTL 

« Hide

Isoform 2 (Soluble CRLM-2) [UniParc].

Checksum: D4382C3273C1B8CA
Show »

FASTA21723,086
Isoform 3 [UniParc].

Checksum: 1063D4172A8BE320
Show »

FASTA37038,834
Isoform 4 [UniParc].

Checksum: BD387A6F9B4CEDF7
Show »

FASTA12613,236

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of CRLM-2, a novel type I cytokine receptor preferentially expressed in hematopoietic cells."
Hiroyama T., Iwama A., Morita Y., Nakamura Y., Shibuya A., Nakauchi H.
Biochem. Biophys. Res. Commun. 272:224-229(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Embryo.
[2]"Molecular cloning of a novel type I cytokine receptor similar to the common gamma chain."
Fujio K., Nosaka T., Kojima T., Kawashima T., Yahata T., Copeland N.G., Gilbert D.J., Jenkins N.A., Yamamoto K., Nishimura T., Kitamura T.
Blood 95:2204-2210(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING.
Tissue: Lymphocyte.
[3]"Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor. Formation Of a functional heteromeric complex requires interleukin 7 receptor."
Park L.S., Martin U., Garka K., Gliniak B., Di Santo J.P., Muller W., Largaespada D.A., Copeland N.G., Jenkins N.A., Farr A.G., Ziegler S.F., Morrissey P.J., Paxton R., Sims J.E.
J. Exp. Med. 192:659-670(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION AS A RECEPTOR FOR TSLP.
Strain: C57BL/6.
Tissue: Lymphocyte.
[4]"Cloning of a receptor subunit required for signaling by thymic stromal lymphopoietin."
Pandey A., Ozaki K., Baumann H., Levin S.D., Puel A., Farr A.G., Ziegler S.F., Leonard W.J., Lodish H.F.
Nat. Immunol. 1:59-64(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Strain: C57BL/6J.
Tissue: Embryonic stem cell.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB039945 mRNA. Translation: BAA92684.1.
AB031333 mRNA. Translation: BAA92159.1.
AF232936 mRNA. Translation: AAF81676.1.
AF201963 mRNA. Translation: AAF82189.1.
AK010291 mRNA. Translation: BAB26827.2.
BC023788 mRNA. Translation: AAH23788.1.
CCDSCCDS19521.1. [Q8CII9-1]
PIRJC7280.
RefSeqNP_001158207.1. NM_001164735.1.
NP_057924.3. NM_016715.4.
UniGeneMm.35771.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4NN5X-ray1.90C20-222[»]
4NN6X-ray2.54C20-222[»]
4NN7X-ray3.78C20-222[»]
ProteinModelPortalQ8CII9.
SMRQ8CII9. Positions 28-206.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid208364. 1 interaction.
DIPDIP-59471N.

PTM databases

PhosphoSiteQ8CII9.

Proteomic databases

PaxDbQ8CII9.
PRIDEQ8CII9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000044579; ENSMUSP00000036326; ENSMUSG00000033467.
GeneID57914.
KEGGmmu:57914.

Organism-specific databases

CTD64109.
MGIMGI:1889506. Crlf2.

Phylogenomic databases

eggNOGNOG74051.
GeneTreeENSGT00510000049974.
HOGENOMHOG000185192.
HOVERGENHBG051120.
KOK05078.
OrthoDBEOG71K63B.
PhylomeDBQ8CII9.
TreeFamTF342693.

Gene expression databases

BgeeQ8CII9.
CleanExMM_CRLF2.
GenevestigatorQ8CII9.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
[Graphical view]
PfamPF00041. fn3. 1 hit.
[Graphical view]
SMARTSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMSSF49265. SSF49265. 2 hits.
PROSITEPS50853. FN3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio314093.
PROQ8CII9.
SOURCESearch...

Entry information

Entry nameCRLF2_MOUSE
AccessionPrimary (citable) accession number: Q8CII9
Secondary accession number(s): Q9CRJ6 expand/collapse secondary AC list , Q9JIE7, Q9JIQ7, Q9JJH8, Q9JMD5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 7, 2003
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot