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Protein

Cytokine receptor-like factor 2

Gene

Crlf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2. Implicated in the development of the hematopoietic system.1 Publication

GO - Biological processi

  • immunoglobulin secretion involved in immune response Source: MGI
  • inflammatory response Source: MGI
  • T-helper 2 cell cytokine production Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Cytokine receptor-like factor 2
Alternative name(s):
Cytokine receptor-like molecule 2
Short name:
CRLM-2
Thymic stromal lymphopoietin protein receptor
Short name:
TSLP receptor
Type I cytokine receptor delta 1
Gene namesi
Name:Crlf2
Synonyms:Crlm2, Tpte2, Tslpr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1889506. Crlf2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 232ExtracellularSequence analysisAdd BLAST213
Transmembranei233 – 253HelicalSequence analysisAdd BLAST21
Topological domaini254 – 359CytoplasmicSequence analysisAdd BLAST106

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001104220 – 359Cytokine receptor-like factor 2Add BLAST340

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi53N-linked (GlcNAc...)1 Publication1
Disulfide bondi68 ↔ 821 Publication
Glycosylationi122N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi168 ↔ 1691 Publication
Disulfide bondi181 ↔ 2191 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ8CII9.
PaxDbiQ8CII9.
PRIDEiQ8CII9.

PTM databases

iPTMnetiQ8CII9.
PhosphoSitePlusiQ8CII9.

Expressioni

Tissue specificityi

High level of expression in liver, lung and testis. Also expressed in heart, brain, spleen, thymus and bone marrow. Highly expressed in progenitors and myeloid cells. Isoform 2 is expressed in primary hemotopoietic cells.

Inductioni

Up-regulated in the myeloid 32D cell line by granulocyte colony-stimulating factor (G-CSF).

Gene expression databases

BgeeiENSMUSG00000033467.
CleanExiMM_CRLF2.

Interactioni

Subunit structurei

The TSLP receptor is a heterodimer of CRLF2 and IL7R. Binding of TSLP to CRLF2/TSLPR is a mechanistic prerequisite for recruitment of IL7R to the high-affinity ternary complex.1 Publication

Protein-protein interaction databases

BioGridi208364. 1 interactor.
DIPiDIP-59471N.
STRINGi10090.ENSMUSP00000036326.

Structurei

Secondary structure

1359
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 34Combined sources5
Turni35 – 37Combined sources3
Beta strandi38 – 42Combined sources5
Beta strandi55 – 60Combined sources6
Beta strandi69 – 73Combined sources5
Beta strandi79 – 85Combined sources7
Beta strandi88 – 90Combined sources3
Beta strandi92 – 97Combined sources6
Beta strandi103 – 109Combined sources7
Helixi111 – 114Combined sources4
Beta strandi124 – 127Combined sources4
Beta strandi133 – 136Combined sources4
Beta strandi141 – 143Combined sources3
Beta strandi145 – 153Combined sources9
Beta strandi162 – 167Combined sources6
Beta strandi170 – 173Combined sources4
Beta strandi180 – 190Combined sources11
Helixi192 – 195Combined sources4
Beta strandi208 – 214Combined sources7
Helixi216 – 218Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NN5X-ray1.90C20-222[»]
4NN6X-ray2.54C20-222[»]
4NN7X-ray3.78C20-222[»]
ProteinModelPortaliQ8CII9.
SMRiQ8CII9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini119 – 213Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST95

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 205WSXWS motif5
Motifi262 – 270Box 1 motif9

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IKHC. Eukaryota.
ENOG41116BS. LUCA.
HOGENOMiHOG000185192.
HOVERGENiHBG051120.
InParanoidiQ8CII9.
KOiK05078.
PhylomeDBiQ8CII9.
TreeFamiTF342693.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q8CII9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAWALAVILL PRLLAAAAAA AAVTSRGDVT VVCHDLETVE VTWGSGPDHH
60 70 80 90 100
GANLSLEFRY GTGALQPCPR YFLSGAGVTS GCILPAARAG LLELALRDGG
110 120 130 140 150
GAMVFKARQR ASAWLKPRPP WNVTLLWTPD GDVTVSWPAH SYLGLDYEVQ
160 170 180 190 200
HRESNDDEDA WQTTSGPCCD LTVGGLDPAR CYDFRVRASP RAAHYGLEAQ
210 220 230 240 250
PSEWTAVTRL SGAASAASCT ASPAPSPALA PPLLPLGCGL AALLTLSLLL
260 270 280 290 300
AALRLRRVKD ALLPCVPDPS GSFPGLFEKH HGNFQAWIAD AQATAPPART
310 320 330 340 350
EEEDDLIHPK AKRVEPEDGT SLCTVPRPPS FEPRGPGGGA MVSVGGATFM

VGDSGYMTL
Length:359
Mass (Da):37,762
Last modified:November 7, 2003 - v2
Checksum:iF9C521C54B4AC9DD
GO
Isoform 2 (identifier: Q8CII9-2) [UniParc]FASTAAdd to basket
Also known as: Soluble CRLM-2

The sequence of this isoform differs from the canonical sequence as follows:
     217-217: A → G
     218-359: Missing.

Show »
Length:217
Mass (Da):23,086
Checksum:iD4382C3273C1B8CA
GO
Isoform 3 (identifier: Q8CII9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-217: A → AGDPCAAHLPPL

Note: No experimental confirmation available.
Show »
Length:370
Mass (Da):38,834
Checksum:i1063D4172A8BE320
GO
Isoform 4 (identifier: Q8CII9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-233: Missing.
     234-234: L → M

Note: No experimental confirmation available.
Show »
Length:126
Mass (Da):13,236
Checksum:iBD387A6F9B4CEDF7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15A → T in BAA92159 (PubMed:10733486).Curated1
Sequence conflicti51G → S in AAF82189 (PubMed:10881176).Curated1
Sequence conflicti51G → S in AAH23788 (PubMed:15489334).Curated1
Sequence conflicti87A → G in AAF81676 (PubMed:10974032).Curated1
Sequence conflicti179A → V in BAA92684 (PubMed:10872831).Curated1
Sequence conflicti179A → V in AAF81676 (PubMed:10974032).Curated1
Sequence conflicti309P → T in BAA92684 (PubMed:10872831).Curated1
Sequence conflicti309P → T in AAF81676 (PubMed:10974032).Curated1
Sequence conflicti309P → T in BAB26827 (PubMed:16141072).Curated1
Sequence conflicti355G → R in BAB26827 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189901 – 233Missing in isoform 4. 1 PublicationAdd BLAST233
Alternative sequenceiVSP_008788217A → G in isoform 2. 1 Publication1
Alternative sequenceiVSP_008790217A → AGDPCAAHLPPL in isoform 3. 1 Publication1
Alternative sequenceiVSP_008789218 – 359Missing in isoform 2. 1 PublicationAdd BLAST142
Alternative sequenceiVSP_018991234L → M in isoform 4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039945 mRNA. Translation: BAA92684.1.
AB031333 mRNA. Translation: BAA92159.1.
AF232936 mRNA. Translation: AAF81676.1.
AF201963 mRNA. Translation: AAF82189.1.
AK010291 mRNA. Translation: BAB26827.2.
BC023788 mRNA. Translation: AAH23788.1.
CCDSiCCDS19521.1. [Q8CII9-1]
PIRiJC7280.
RefSeqiNP_001158207.1. NM_001164735.1.
NP_057924.3. NM_016715.4.
UniGeneiMm.35771.

Genome annotation databases

GeneIDi57914.
KEGGimmu:57914.
UCSCiuc008ypg.2. mouse. [Q8CII9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039945 mRNA. Translation: BAA92684.1.
AB031333 mRNA. Translation: BAA92159.1.
AF232936 mRNA. Translation: AAF81676.1.
AF201963 mRNA. Translation: AAF82189.1.
AK010291 mRNA. Translation: BAB26827.2.
BC023788 mRNA. Translation: AAH23788.1.
CCDSiCCDS19521.1. [Q8CII9-1]
PIRiJC7280.
RefSeqiNP_001158207.1. NM_001164735.1.
NP_057924.3. NM_016715.4.
UniGeneiMm.35771.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NN5X-ray1.90C20-222[»]
4NN6X-ray2.54C20-222[»]
4NN7X-ray3.78C20-222[»]
ProteinModelPortaliQ8CII9.
SMRiQ8CII9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208364. 1 interactor.
DIPiDIP-59471N.
STRINGi10090.ENSMUSP00000036326.

PTM databases

iPTMnetiQ8CII9.
PhosphoSitePlusiQ8CII9.

Proteomic databases

EPDiQ8CII9.
PaxDbiQ8CII9.
PRIDEiQ8CII9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi57914.
KEGGimmu:57914.
UCSCiuc008ypg.2. mouse. [Q8CII9-1]

Organism-specific databases

CTDi64109.
MGIiMGI:1889506. Crlf2.

Phylogenomic databases

eggNOGiENOG410IKHC. Eukaryota.
ENOG41116BS. LUCA.
HOGENOMiHOG000185192.
HOVERGENiHBG051120.
InParanoidiQ8CII9.
KOiK05078.
PhylomeDBiQ8CII9.
TreeFamiTF342693.

Miscellaneous databases

PROiQ8CII9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033467.
CleanExiMM_CRLF2.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRLF2_MOUSE
AccessioniPrimary (citable) accession number: Q8CII9
Secondary accession number(s): Q9CRJ6
, Q9JIE7, Q9JIQ7, Q9JJH8, Q9JMD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 7, 2003
Last modified: November 30, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.