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Protein

Nesprin-4

Gene

Syne4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning (By similarity). Behaves as a kinesin cargo, providing a functional binding site for kinesin-1 at the nuclear envelope. Hence may contribute to the establishment of secretory epithelial morphology, by promoting kinesin-dependent apical migration of the centrosome and Golgi apparatus and basal localization of the nucleus.By similarity1 Publication

GO - Biological processi

  • establishment of epithelial cell apical/basal polarity Source: UniProtKB
  • mitotic spindle organization Source: GO_Central
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Nesprin-4
Alternative name(s):
Nuclear envelope spectrin repeat protein 4
Gene namesi
Name:Syne4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2141950. Syne4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 339339CytoplasmicPROSITE-ProRule annotationAdd
BLAST
Transmembranei340 – 36021Helical; Anchor for type IV membrane proteinPROSITE-ProRule annotationAdd
BLAST
Topological domaini361 – 38828Perinuclear spacePROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • integral component of nuclear outer membrane Source: UniProtKB
  • LINC complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate and look overtly normal. In the cochlea, outer hair cells form, but appear to degenerate as hearing matures. Inner hair cells remain intact. Hearing loss is already detected at P15 and progresses at all frequencies by P60.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Nesprin-4PRO_0000306265Add
BLAST

Proteomic databases

PaxDbiQ8CII8.
PRIDEiQ8CII8.

PTM databases

iPTMnetiQ8CII8.
PhosphoSiteiQ8CII8.

Expressioni

Tissue specificityi

Expressed in secretory epithelial cells, such as those found in exocrine pancreas, bulbourethral gland, mammary gland and salivary gland (at protein level). Also expressed in the cochlea, where it is restricted primarily to the 3 rows of outer hair cells and 1 row of inner hair cells (at protein level). Not detected in other cells of the cochlea, including Deiter's cells and pillar cells, nor in liver and kidney (at protein level).2 Publications

Developmental stagei

In the cochlea, low expression at P0 and P15, then rises significantly by P30 and remains steady. In the HC11 cell line model, up-regulated during differentation of mammary cells into milk-secreting cells (at protein level).1 Publication

Gene expression databases

BgeeiQ8CII8.
CleanExiMM_AI428936.
ExpressionAtlasiQ8CII8. baseline and differential.
GenevisibleiQ8CII8. MM.

Interactioni

Subunit structurei

Core component of the LINC complex which is composed of inner nuclear membrane SUN domain-containing proteins coupled to outer nuclear membrane KASH domain-containing nesprins. SUN domain-containing proteins interact with A-type lamins of the nuclear lamina, while at the other end of the complex, nesprins interact with unique cytoskeletal components (By similarity). Interacts with kinesins KIF5B and KLC1.By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-48702N.
STRINGi10090.ENSMUSP00000055874.

Structurei

3D structure databases

ProteinModelPortaliQ8CII8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini331 – 38858KASHPROSITE-ProRule annotationAdd
BLAST

Domaini

The KASH domain, which contains a transmembrane domain, mediates the nuclear envelope targeting and is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.1 Publication

Sequence similaritiesi

Belongs to the nesprin family.Curated
Contains 1 KASH domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIPP. Eukaryota.
ENOG4111B09. LUCA.
GeneTreeiENSGT00510000049061.
HOGENOMiHOG000111992.
HOVERGENiHBG107763.
InParanoidiQ8CII8.
OMAiSHRKHLA.
OrthoDBiEOG7NW69N.
PhylomeDBiQ8CII8.

Family and domain databases

InterProiIPR012315. KASH.
IPR030268. SYNE4.
[Graphical view]
PANTHERiPTHR21640:SF1. PTHR21640:SF1. 1 hit.
PfamiPF10541. KASH. 1 hit.
[Graphical view]
SMARTiSM01249. KASH. 1 hit.
[Graphical view]
PROSITEiPS51049. KASH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CII8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVPPLGRE FPPEPVNCPL AAPRELDVVG GTICPAPEEE TSRPEQVQAS
60 70 80 90 100
LGLPEHCMGE LKSTESATSP SRLPLASSHE HQDGGKPCEH SDSGLEVLEA
110 120 130 140 150
EQDSLHLCLL RLNFRLQDLE RGLGSWTLAH NRIVQMQALQ AELRGAAERV
160 170 180 190 200
DALLAFGEGL AERSEPRAWA SLEQVLRALG THRDTIFQRL WQLQAQLISY
210 220 230 240 250
SLVLEKANLL DQDLEVEGDS DGPAAGGVWG PWAPSTFPTP AELEWDPAGD
260 270 280 290 300
VGGLGPSGQK ISRIPGAPCE LCGYRGPQSS GQGLEDLLSL GLGHRKHLAA
310 320 330 340 350
HHRRRLRKPQ DRKRQVSPSL PDAMLEVDRG VPAPASKRPL TLFFLLLFLL
360 370 380
LVGATLLLPL SGVSCCSHAR LARTPYLVLS YVNGLPPI
Length:388
Mass (Da):42,024
Last modified:March 1, 2003 - v1
Checksum:iD407808F62CB5498
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561H → Q in AAH04761 (PubMed:15489334).Curated
Sequence conflicti56 – 561H → Q in AAH56649 (PubMed:15489334).Curated
Sequence conflicti239 – 2391T → I in AAH56649 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC004761 mRNA. Translation: AAH04761.2.
BC023803 mRNA. Translation: AAH23803.1.
BC056649 mRNA. Translation: AAH56649.1.
CCDSiCCDS21086.1.
RefSeqiNP_705805.1. NM_153577.2.
UniGeneiMm.227325.

Genome annotation databases

EnsembliENSMUST00000054594; ENSMUSP00000055874; ENSMUSG00000019737.
GeneIDi233066.
KEGGimmu:233066.
UCSCiuc009gec.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC004761 mRNA. Translation: AAH04761.2.
BC023803 mRNA. Translation: AAH23803.1.
BC056649 mRNA. Translation: AAH56649.1.
CCDSiCCDS21086.1.
RefSeqiNP_705805.1. NM_153577.2.
UniGeneiMm.227325.

3D structure databases

ProteinModelPortaliQ8CII8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48702N.
STRINGi10090.ENSMUSP00000055874.

PTM databases

iPTMnetiQ8CII8.
PhosphoSiteiQ8CII8.

Proteomic databases

PaxDbiQ8CII8.
PRIDEiQ8CII8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054594; ENSMUSP00000055874; ENSMUSG00000019737.
GeneIDi233066.
KEGGimmu:233066.
UCSCiuc009gec.1. mouse.

Organism-specific databases

CTDi163183.
MGIiMGI:2141950. Syne4.

Phylogenomic databases

eggNOGiENOG410IIPP. Eukaryota.
ENOG4111B09. LUCA.
GeneTreeiENSGT00510000049061.
HOGENOMiHOG000111992.
HOVERGENiHBG107763.
InParanoidiQ8CII8.
OMAiSHRKHLA.
OrthoDBiEOG7NW69N.
PhylomeDBiQ8CII8.

Miscellaneous databases

PROiQ8CII8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CII8.
CleanExiMM_AI428936.
ExpressionAtlasiQ8CII8. baseline and differential.
GenevisibleiQ8CII8. MM.

Family and domain databases

InterProiIPR012315. KASH.
IPR030268. SYNE4.
[Graphical view]
PANTHERiPTHR21640:SF1. PTHR21640:SF1. 1 hit.
PfamiPF10541. KASH. 1 hit.
[Graphical view]
SMARTiSM01249. KASH. 1 hit.
[Graphical view]
PROSITEiPS51049. KASH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.
  2. "Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization."
    Roux K.J., Crisp M.L., Liu Q., Kim D., Kozlov S., Stewart C.L., Burke B.
    Proc. Natl. Acad. Sci. U.S.A. 106:2194-2199(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KIF5B AND KLC1, SUBCELLULAR LOCATION, TOPOLOGY, DOMAIN, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  3. Cited for: DISRUPTION PHENOTYPE, POTENTIAL INTERACTION WITH SUN1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSYNE4_MOUSE
AccessioniPrimary (citable) accession number: Q8CII8
Secondary accession number(s): Q6PH99, Q99J42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.